Cross infection control measures and the treatment of patients at risk of Creutzfeldt Jakob disease in UK general dental practice

AIMS: To determine the suitability of key infection control measures currently employed in UK dental practice for delivery of dental care to patients at risk of prion diseases. MATERIALS AND METHODS: Subjects: Five hundred dental surgeons currently registered with the General Dental Council of the UK. Data collection: Structured postal questionnaire. Analysis: Frequencies, cross-tabulations and chi-squared analysis. RESULTS: The valid response rate to the questionnaire was 69%. 33% of practices had no policy on general disinfection and sterilisation procedures. Only 10 of the 327 responding practices (3%) possessed a vacuum autoclave. 49% of dentists reported using the BDA medical history form but less than 25% asked the specific questions recommended by the BDA to identify patients at risk of iatrogenic or familial CJD. However, 63% of practitioners would refer such patients, if identified, to a secondary care facility. Of the 107 practitioners who were prepared to provide dental treatment, 75 (70%) would do so using routine infection control procedures. CONCLUSIONS: Most of the dental practices surveyed were not actively seeking to identify patients at risk of prion diseases. In many cases, recommended procedures for providing safe dental care for such patients were not in place

Endometrial stromal cells of women with recurrent miscarriage fail to discriminate between high- and low-quality human embryos

Background The aetiology of recurrent miscarriage (RM) remains largely unexplained. Women with RM have a shorter time to pregnancy interval than normally fertile women, which may be due to more frequent implantation of non-viable embryos. We hypothesized that human endometrial stromal cells (H-EnSCs) of women with RM discriminate less effectively between high-and low-quality human embryos and migrate more readily towards trophoblast spheroids than H-EnSCs of normally fertile women. Methodology/Principal Findings Monolayers of decidualized H-EnSCs were generated from endometrial biopsies of 6 women with RM and 6 fertile controls. Cell-free migration zones were created and the effect of the presence of a high-quality (day 5 blastocyst, n = 13), a low-quality (day 5 blastocyst with three pronuclei or underdeveloped embryo, n = 12) or AC-1M88 trophoblast cell line spheroid on H-ESC migratory activity was analyzed after 18 hours. In the absence of a spheroid or embryo, migration of H-EnSCs from fertile or RM women was similar. In the presence of a low-quality embryo in the zone, the migration of H-EnSCs of control women was inhibited compared to the basal migration in the absence of an embryo (P<0.05) and compared to the migration in the presence of high-quality embryo (p<0.01). Interestingly, the migratory response H-EnSCs of women with RM did not differ between high- and low-quality embryos. Furthermore, in the presence of a spheroid their migration was enhanced compared to the H-EnSCs of controls (p<0.001). Conclusions H-EnSCs of fertile women discriminate between high- and low-quality embryos whereas H-EnSCs of women with RM fail to do so. H-EnSCs of RM women have a higher migratory response to trophoblast spheroids. Future studies will focus on the mechanisms by which low-quality embryos inhibit the migration of H-EnSCs and how this is deregulated in women with RM

Sharing brain mapping statistical results with the neuroimaging data model

Only a tiny fraction of the data and metadata produced by an fMRI study is finally conveyed to the community. This lack of transparency not only hinders the reproducibility of neuroimaging results but also impairs future meta-analyses. In this work we introduce NIDM-Results, a format specification providing a machine-readable description of neuroimaging statistical results along with key image data summarising the experiment. NIDM-Results provides a unified representation of mass univariate analyses including a level of detail consistent with available best practices. This standardized representation allows authors to relay methods and results in a platform-independent regularized format that is not tied to a particular neuroimaging software package. Tools are available to export NIDM-Result graphs and associated files from the widely used SPM and FSL software packages, and the NeuroVault repository can import NIDM-Results archives. The specification is publically available at: http://nidm.nidash.org/specs/nidm-results.html

Age-related changes in global motion coherence: conflicting haemodynamic and perceptual responses

Our aim was to use both behavioural and neuroimaging data to identify indicators of perceptual decline in motion processing. We employed a global motion coherence task and functional Near Infrared Spectroscopy (fNIRS). Healthy adults (n = 72, 18-85) were recruited into the following groups: young (n = 28, mean age = 28), middle-aged (n = 22, mean age = 50), and older adults (n = 23, mean age = 70). Participants were assessed on their motion coherence thresholds at 3 different speeds using a psychophysical design. As expected, we report age group differences in motion processing as demonstrated by higher motion coherence thresholds in older adults. Crucially, we add correlational data showing that global motion perception declines linearly as a function of age. The associated fNIRS recordings provide a clear physiological correlate of global motion perception. The crux of this study lies in the robust linear correlation between age and haemodynamic response for both measures of oxygenation. We hypothesise that there is an increase in neural recruitment, necessitating an increase in metabolic need and blood flow, which presents as a higher oxygenated haemoglobin response. We report age-related changes in motion perception with poorer behavioural performance (high motion coherence thresholds) associated with an increased haemodynamic response

The first report of RPSA polymorphisms, also called 37/67 kDa LRP/LR gene, in sporadic Creutzfeldt-Jakob disease (CJD)

<p>Abstract</p> <p>Background</p> <p>Although polymorphisms of <it>PRNP</it>, the gene encoding prion protein, are known as a determinant affecting prion disease susceptibility, other genes also influence prion incubation time. This finding offers the opportunity to identify other genetic or environmental factor (s) modulating susceptibility to prion disease. Ribosomal protein SA (<it>RPSA</it>), also called 37 kDa laminin receptor precursor (LRP)/67 kDa laminin receptor (LR), acts as a receptor for laminin, viruses and prion proteins. The binding/internalization of prion protein is dependent for LRP/LR.</p> <p>Methods</p> <p>To identify other susceptibility genes involved in prion disease, we performed genetic analysis of <it>RPSA</it>. For this case-control study, we included 180 sporadic Creutzfeldt-Jakob disease (CJD) patients and 189 healthy Koreans. We investigated genotype and allele frequencies of polymorphism on <it>RPSA </it>by direct sequencing or restriction fragment length polymorphism (RFLP) analysis.</p> <p>Results</p> <p>We observed four single nucleotide polymorphisms (SNPs), including -8T>C (rs1803893) in the 5'-untranslated region (UTR) of exon 2, 134-32C>T (rs3772138) in the intron, 519G>A (rs2269350) in the intron and 793+58C>T (rs2723) in the intron on the <it>RPSA</it>. The 519G>A (at codon 173) is located in the direct PrP binding site. The genotypes and allele frequencies of the <it>RPSA </it>polymorphisms showed no significant differences between the controls and sporadic CJD patients.</p> <p>Conclusion</p> <p>These results suggest that these <it>RPSA </it>polymorphisms have no direct influence on the susceptibility to sporadic CJD. This was the first genetic association study of the polymorphisms of <it>RPSA </it>gene with sporadic CJD.</p

Autoimmune diseases and pregnancy: analysis of a series of cases

BACKGROUND: An autoimmune disease is characterized by tissue damage, caused by self-reactivity of different effector mechanisms of the immune system, namely antibodies and T cells. All autoimmune diseases, to some extent, have implications for fertility and obstetrics. Currently, due to available treatments and specialised care for pregnant women with autoimmune disease, the prognosis for both mother and child has improved significantly. However these pregnancies are always high risk. The purpose of this study is to analyse the fertility/pregnancy process of women with systemic and organ-specific autoimmune diseases and assess pathological and treatment implications. METHODS: The authors performed an analysis of the clinical records and relevant obstetric history of five patients representing five distinct autoimmune pathological scenarios, selected from Autoimmune Disease Consultation at the Hospital of Braga, and reviewed the literature. RESULTS: The five clinical cases are the following: Case 1-28 years old with systemic lupus erythematosus, and clinical remission of the disease, under medication with hydroxychloroquine, prednisolone and acetylsalicylic acid, with incomplete miscarriage at 7 weeks of gestation without signs of thrombosis. Case 2-44 years old with history of two late miscarriages, a single preterm delivery (33 weeks) and multiple thrombotic events over the years, was diagnosed with antiphospholipid syndrome after acute myocardial infarction. Case 3-31 years old with polymyositis, treated with azathioprine for 3 years with complete remission of the disease, took the informed decision to get pregnant after medical consultation and full weaning from azathioprine, and gave birth to a healthy term new-born. Case 4-38 years old pregnant woman developed Behcet's syndrome during the final 15 weeks of gestation and with disease exacerbation after delivery. Case 5-36 years old with autoimmune thyroiditis diagnosed during her first pregnancy, with difficult control over the thyroid function over the years and first trimester miscarriage, suffered a second miscarriage despite clinical stability and antibody regression. CONCLUSIONS: As described in literature, the authors found a strong association between autoimmune disease and obstetric complications, especially with systemic lupus erythematosus, antiphospholipid syndrome and autoimmune thyroiditis

A Review of Economic Consequences and Costs of Male Violence Against Women

[EN] This article focuses on male violence against women. As it takes place in what is often considered to be 'the private sphere' of the home, violence is difficult to prove, to measure, to prevent and easy to ignore. A multi-country study (WHO, 2005, WHO multi-country study on women's health and domestic violence against women: Summary report of initial results on prevalence, health outcomes and women's responses, Geneva, Switzerland: World Health Organization) shows that there are wide variations between countries resulting in 15 per cent to 71 per cent of women aged between 15 and 49 years saying that they have been victims of physical or sexual violence in intimate relationships. This article reviews and summarises literature that analyse types of economic costs that result from domestic violence and abuse perpetrated against women.The theoretical reflections and findings are from a research project funded by the European Commission through the Leonardo da Vinci Programme named 'Giving Hope to Victims of Abuse through Vocational Guidance', promoted and coordinated by the University Miguel Hernandez of Elche. The content of this paper does not necessarily reflect the position of the European Union or the National Agency, nor does it involve any responsibility on their part (Agreement number: 2011/3500-516610-LLp-1-2011-1-ES-LEONARDO-LMP).López-Sánchez, MJ.; Belso-Martínez, JA.; Hervás Oliver, JL. (2019). A Review of Economic Consequences and Costs of Male Violence Against Women. Indian Journal of Gender Studies. 26(3):424-434. https://doi.org/10.1177/0971521519861194S424434263Babcock, J. C., Waltz, J., Jacobson, N. S., & Gottman, J. M. (1993). Power and violence: The relation between communication patterns, power discrepancies, and domestic violence. Journal of Consulting and Clinical Psychology, 61(1), 40-50. doi:10.1037/0022-006x.61.1.40Bloch, F., & Rao, V. (2002). Terror as a Bargaining Instrument: A Case Study of Dowry Violence in Rural India. American Economic Review, 92(4), 1029-1043. doi:10.1257/00028280260344588Comijs, H. C., Pot, A. M., Smit, J. H., Bouter, L. M., & Jonker, C. (1998). Elder Abuse in the Community: Prevalence and Consequences. Journal of the American Geriatrics Society, 46(7), 885-888. doi:10.1111/j.1532-5415.1998.tb02724.xFord-Gilboe, M., Wuest, J., & Merritt-Gray, M. (2005). Strengthening Capacity to Limit Intrusion: Theorizing Family Health Promotion in the Aftermath of Woman Abuse. Qualitative Health Research, 15(4), 477-501. doi:10.1177/1049732305274590Garbarino, J., & Crouter, A. (1978). Defining the Community Context for Parent-Child Relations: The Correlates of Child Maltreatment. Child Development, 49(3), 604. doi:10.2307/1128227Grana, S. J. (2001). Journal of Family Violence, 16(4), 421-435. doi:10.1023/a:1012229011161HEISE, L. L. (1998). Violence Against Women. Violence Against Women, 4(3), 262-290. doi:10.1177/1077801298004003002Kim, J., & Gray, K. A. (2008). Leave or Stay? Journal of Interpersonal Violence, 23(10), 1465-1482. doi:10.1177/0886260508314307Krug, E. G., Mercy, J. A., Dahlberg, L. L., & Zwi, A. B. (2002). The world report on violence and health. The Lancet, 360(9339), 1083-1088. doi:10.1016/s0140-6736(02)11133-0LAMBERT, L. C., & FIRESTONE, J. M. (2000). Economic Context and Multiple Abuse Techniques. Violence Against Women, 6(1), 49-67. doi:10.1177/1077801200006001004Max, W., Rice, D. P., Finkelstein, E., Bardwell, R. A., & Leadbetter, S. (2004). The Economic Toll of Intimate Partner Violence Against Women in the United States. Violence and Victims, 19(3), 259-272. doi:10.1891/vivi.19.3.259.65767(2003). Costs of Intimate Partner Violence Against Women in the United States. PsycEXTRA Dataset. doi:10.1037/e721242007-001Reeves, C., & O’Leary-Kelly, A. M. (2007). The Effects and Costs of Intimate Partner Violence for Work Organizations. Journal of Interpersonal Violence, 22(3), 327-344. doi:10.1177/0886260506295382Roldós, M. I., & Corso, P. (2013). The Economic Burden of Intimate Partner Violence in Ecuador: Setting the Agenda for Future Research and Violence Prevention Policies. Western Journal of Emergency Medicine, 14(4), 347-353. doi:10.5811/westjem.2013.2.15697Schiamberg, L. B., & Gans, D. (1999). An Ecological Framework for Contextual Risk Factors in Elder Abuse by Adult Children. Journal of Elder Abuse & Neglect, 11(1), 79-103. doi:10.1300/j084v11n01_05(1993). World Development Report 1993. doi:10.1596/0-1952-0890-0TOLMAN, R. M., & ROSEN, D. (2001). Domestic Violence in the Lives of Women Receiving Welfare. Violence Against Women, 7(2), 141-158. doi:10.1177/1077801201007002003Wuest, J., Ford-Gilboe, M., Merritt-Gray, M., & Berman, H. (2003). Intrusion: The Central Problem for Family Health Promotion among Children and Single Mothers after Leaving an Abusive Partner. Qualitative Health Research, 13(5), 597-622. doi:10.1177/1049732303013005002Yodanis, C. L., Godenzi, A., & Stanko, E. A. (2000). The Benefits of Studying Costs: A Review and Agenda for Studies on the Economic Costs of Violence Against Women. Policy Studies, 21(3), 263-276. doi:10.1080/0144287002001953

Optical imaging and spectroscopy for the study of the human brain: status report

This report is the second part of a comprehensive two-part series aimed at reviewing an extensive and diverse toolkit of novel methods to explore brain health and function. While the first report focused on neurophotonic tools mostly applicable to animal studies, here, we highlight optical spectroscopy and imaging methods relevant to noninvasive human brain studies. We outline current state-of-the-art technologies and software advances, explore the most recent impact of these technologies on neuroscience and clinical applications, identify the areas where innovation is needed, and provide an outlook for the future directions