Gwyneth Walker’s use of melodic motive and tonal centricity to depict E. E. Cummings’ Poetry in the Cycle “though love be a day.”

I. Opera Role: Thursday, April 11, 2013, 7:30 p.m., Taylor Theatre. Noémie in Cendrillon (Jules Massenet) II. Solo Recital: Sunday, April 21, 2013, 5:30 p.m., Recital Hall. "The Blessed Virgin's Expostulation" (Henry Purcell, realized by Benjamin Britten); "Suleika I" D. 720 , "Suleika II" D. 717 (Franz Schubert); "Nur wer die Sehnuscht kennt," "Heiβ mich nicht redden, heiβ mich schweigen," "So laβt mich scheinen, bis ich werde" from Lieder und Gesänge aus Wilhelm Meister, op. 98a (Robert Schumann); "Solveigs Sang," "Solveigs Vuggesang" from Peer Gynt, op. 23 (Edvard Grieg); Try Me, Good King Last Words of the Wives of Henvry VIII (Libby Larsen) III. Solo Recital: Sunday, April 13, 2014, 5:30 p.m., Recital Hall. "Wir eilen mit schwachen, doch emsigen Schritten" from Jesu, der du meine Seele, BWV 78 (Johann Sebastian Bach); "Wenn des Kreuzes Bitterkeiten" from Was Gott tut, das ist wohlgetan, BWV 99 (Johann Sebastian Bach); "Слезы," "В огороде, возле броду," "Рассвет" from Шесть Дуэтов (Six Duets), op. 46 (Pyotr Ilyich Tchaikovsky); "La pesca" from Soirées musicales (Gioacchino Rossini), "Le gittane" from Péchés de vieillesse (Gioacchino Rossini); Pavane, op. 50 (Gabriel Fauré), El desdichado (Camille Saint-Saëns); Chanson Espagnole (Claude Debussy); "Rede, Mädchen, allzu liebes," "Die grüne Hopfenranke," "Ein kleiner, hübscher Vogel," "Wenn so lind dein Aug emir," "Am Donaustrande, da steht ein Haus," "Nein, es ist nicht auszukommen," "Schlosser auf! Und mache Schlösser," "Ein dunkeler Schacht ist Liebe," "Es bebet das Gesträuche" from Liebeslieder Walzer, op. 52 (Johannes Brahms) IV. Solo Recital: Sunday, October 26, 2014, 5:30 p.m., Recital Hall. The Telephone (Gian-Carlo Menotti) V. D.M.A. Research Project. GWYNETH WALKER'S USE OF MELODIC MOTIVE AND TONAL CENTIRICITY TO DEPICT E. E. CUMMINGS' POETRY IN THE CYCLE "THOUGH LOVE BE A DAY." This document provides an overview of E. E. Cummings' and Gwyneth Walker's biographical information and artistic output before analyzing the harmonic and melodic motives Walker uses in the four settings of E. E. Cummings poetry found in this song cycle

Why not "do simple things in a simple way": Use of the Pap test as the first step in screening genetic stability for human cultured stem cell therapy?

The aim of this study was to analyze adipose tissue-derived mesenchymal stem cells (AT-MSCs) using the Pap test as a first screening step to evaluate genetic stability. Human adipose tissue from six healthy female donors was obtained from elective liposuction procedures. The cells were isolated, cultivated at P2/P3, characterized by flow cytometric analysis, and differentiation induced. The AT-MSCs were stained by Papanicolaou staining and analyzed according to the Bethesda classification, and viability-apoptosis relationships were evaluated. The results of the Pap test for Sample I indicated high-grade alterations consistent with genetic instability; for Samples II-V, atypical cells of undetermined significance; and for Sample VI, normal cells. These results demonstrate the potential of using the Pap test as an initial screening step to evaluate the genetic stability of cultured AT-MSCs and also suggest its use for other adherent cells such as embryonic stem cells or induced pluripotent stem cells

Plasma kinetics of an LDL-like nanoemulsion and lipid transfer to HDL in subjects with glucose intolerance

OBJECTIVE: Glucose intolerance is frequently associated with an altered plasma lipid profile and increased cardiovascular disease risk. Nonetheless, lipid metabolism is scarcely studied in normolipidemic glucose-intolerant patients. The aim of this study was to investigate whether important lipid metabolic parameters, such as the kinetics of LDL free and esterified cholesterol and the transfer of lipids to HDL, are altered in glucose-intolerant patients with normal plasma lipids. METHODS: Fourteen glucose-intolerant patients and 15 control patients were studied; none of the patients had cardiovascular disease manifestations, and they were paired for age, sex, race and co-morbidities. A nanoemulsion resembling a LDL lipid composition (LDE) labeled with 14C-cholesteryl ester and ³H-free cholesterol was intravenously injected, and blood samples were collected over a 24-h period to determine the fractional clearance rate of the labels by compartmental analysis. The transfer of free and esterified cholesterol, triglycerides and phospholipids from the LDE to HDL was measured by the incubation of the LDE with plasma and radioactivity counting of the supernatant after chemical precipitation of non-HDL fractions. RESULTS: The levels of LDL, non-HDL and HDL cholesterol, triglycerides, apo A1 and apo B were equal in both groups. The 14C-esterified cholesterol fractional clearance rate was not different between glucose-intolerant and control patients, but the ³H-free-cholesterol fractional clearance rate was greater in glucose-intolerant patients than in control patients. The lipid transfer to HDL was equal in both groups. CONCLUSION: In these glucose-intolerant patients with normal plasma lipids, a faster removal of LDE free cholesterol was the only lipid metabolic alteration detected in our study. This finding suggests that the dissociation of free cholesterol from lipoprotein particles occurs in normolipidemic glucose intolerance and may participate in atherogenic signaling

Plasma kinetics of an LDL-like nanoemulsion and lipid transfer to HDL in subjects with glucose intolerance

OBJECTIVE: Glucose intolerance is frequently associated with an altered plasma lipid profile and increased cardiovascular disease risk. Nonetheless, lipid metabolism is scarcely studied in normolipidemic glucose-intolerant patients. The aim of this study was to investigate whether important lipid metabolic parameters, such as the kinetics of LDL free and esterified cholesterol and the transfer of lipids to HDL, are altered in glucose-intolerant patients with normal plasma lipids. METHODS: Fourteen glucose-intolerant patients and 15 control patients were studied; none of the patients had cardiovascular disease manifestations, and they were paired for age, sex, race and co-morbidities. A nanoemulsion resembling a LDL lipid composition (LDE) labeled with C-14-cholesteryl ester and H-3-free cholesterol was intravenously injected, and blood samples were collected over a 24-h period to determine the fractional clearance rate of the labels by compartmental analysis. The transfer of free and esterified cholesterol, triglycerides and phospholipids from the LDE to HDL was measured by the incubation of the LDE with plasma and radioactivity counting of the supernatant after chemical precipitation of non-HDL fractions. RESULTS: The levels of LDL, non-HDL and HDL cholesterol, triglycerides, apo A1 and apo B were equal in both groups. The 14 C-esterified cholesterol fractional clearance rate was not different between glucose-intolerant and control patients, but the H-3-free- cholesterol fractional clearance rate was greater in glucose-intolerant patients than in control patients. The lipid transfer to HDL was equal in both groups. CONCLUSION: In these glucose-intolerant patients with normal plasma lipids, a faster removal of LDE free cholesterol was the only lipid metabolic alteration detected in our study. This finding suggests that the dissociation of free cholesterol from lipoprotein particles occurs in normolipidemic glucose intolerance and may participate in atherogenic signaling.This study was supported by Fundação do Amparo à Pesquisa do Estado\ud de São Paulo (FAPESP), São Paulo, Brazil. Dr. Maranhão has a Research\ud Award from Conselho Nacional de Desenvolvimento Científico e\ud Tecnológico (CNPq) Brasília, Brazil. Dr. Bertato had a scholarship from\ud CNPq

Characterization of Acrocarpus fraxinifolius wood submitted to heat treatment

Aiming to provide greater visibility for the wood species Acrocarpus fraxinifolius, the present study sought to analyze the influence of heat treatment on an industrial scale applied to wood species, also popularly known as Indian cedar. The heat treatment was carried out in an autoclave, with temperature and pressure control, and with saturated steam injection, for temperatures 155 ºC, 165 ºC, 175 ºC, and 185 ºC. Physical, chemical, and mechanical tests were carried out for the analyzed wood. The content of holocellulose and total lignin decreased, while the content of extractives showed a substantial increase. The density increased after the heat treatment, however the treated wood showed cracks, and these cracks influenced the significant loss of the values of the mechanical properties of compression, tension, and flexion. The shear showed strength gain for the temperature of 155 ºC, and the wood treated at 165 ºC was equivalent to untreated wood. The woods submitted to temperatures of 175 ºC and 185 ºC presented strength losses. The heat treatment in question contributes to increase the visibility, use and market value of wood

Sward Management Targets in Natural Grasslands of Southern Brazil

The relationship between environment, plant and animal is one of the most important focuses in the ecological and productive context of natural grasslands. The limited knowledge of the complexity of this environment can lead to inappropriate management strategies, determining degradation, biodiversity and productivity losses. This study is based on the concept that the best way to conserve natural grasslands is through adequate management targets. This study aimed to identify sward management targets that maximize productivity in natural grasslands of Southern Brazil

A change in SHATTERPROOF protein lies at the origin of a fruit morphological novelty and a new strategy for seed dispersal in Medicago genus

[EN] Angiosperms are the most diverse and numerous group of plants, and it is generally accepted that this evolutionary success owes in part to the diversity found in fruits, key for protecting the developing seeds and ensuring seed dispersal. Although studies on the molecular basis of morphological innovations are few, they all illustrate the central role played by transcription factors acting as developmental regulators. Here, we show that a small change in the protein sequence of a MADS-box transcription factor correlates with the origin of a highly modified fruit morphology and the change in seed dispersal strategies that occurred in Medicago, a genus belonging to the large legume family. This protein sequence modification alters the functional properties of the protein, affecting the affinities for other protein partners involved in high-order complexes. Our work illustrates that variation in coding regions can generate evolutionary novelties not based on gene duplication/subfunctionalization but by interactions in complex networks, contributing also to the current debate on the relative importance of changes in regulatory or coding regions of master regulators in generating morphological novelties.This work was supported by the Spanish Ministerio de Ciencia e Innovacion (grant no. BIO2009-09920 to C.Fe.), the European Union (grant no. FP7-PEOPLE-PIRSES-2009-247589 to C.Fe. and A.C.d.O.), and a Fellowship for Foreign Young Postdocs from the Spanish Ministerio de Ciencia e Innovacion (to C.Fo.).Fourquin, C.; Del Cerro Fernández, C.; Victoria, FC.; Vialette-Guiraud, A.; De Oliveira, AC.; Ferrandiz Maestre, C. (2013). A change in SHATTERPROOF protein lies at the origin of a fruit morphological novelty and a new strategy for seed dispersal in Medicago genus. Plant Physiology. 162(2):907-917. https://doi.org/10.1104/pp.113.217570S907917162

Optimal control for an irrigation planning problem: characterisation of solution and validation of the numerical results

In a previous study, the authors developed the planning of the water used in the irrigation systems of a given farmland in order to ensure that the field cultivation is in a good state of preservation. This planning was modelled and tackled as an optimal control problem: minimize the water flow (control) so that the extent water amount in the soil (trajectory) fulfils the cultivation water requirements. In this paper, we characterize the solution of our problem guaranteeing the existence of the solution and applying the necessary and sufficient conditions of optimality. We validate the numerical results obtained previously, comparing the analytical and numerical solutions.PEst-OE/MAT/UI0013/2014 and PEST-C/FIS/UI607/2013, European Union FP7 (FP7-PEOPLE-2010-ITN, Grant Agreement no. 264735- SADCO), FCT projects PTDC/EEA-CRO/116014/2009 and PTDC/EEI-AUT/1450/201