Truly Accomplished: An Exploratory Study of Motivation and Social Influence

The purpose of this study was to examine the motivation of individuals to use Truly Accomplished (TA) as a fitness intervention, with or without a social support component. All participants utilized the TA Software as a fitness intervention and received weekly feedback on their progress. Thirty participants were randomly assigned to either participate in the regular TA process (n=16) or a modified TA process (n=14) with the addition of a social support component in the form of a social media group. Participants in the modified condition posted weekly to a Facebook group page about successes and obstacles they faced and gave feedback to their peers. It was hypothesized that participants, regardless of condition, would show fitness gains in the 6-week study. Also, with the added social support component to TA, participants were expected to have higher levels of self-determination in relation to the three psychological needs (i.e. autonomy, relatedness, and competence) and higher levels of perceived social support. A between-subjects deign was used to measure overall effectiveness, changes in fitness performance (plank, push-ups, wall-sit, step-test) and body composition (BMI, percent body fat), perceived social support, levels of psychological needs satisfaction as it relates to the three basic needs (autonomy, competence, and relatedness), and satisfaction with TA. No significant differences were found between conditions for satisfaction with TA, levels of perceived social support, or levels of psychological needs satisfaction post-intervention. However, a significant increase was found regardless of condition in terms of autonomy and guidance. A significant increase was shown in both the plank and modified push-up post-intervention measure, regardless of condition. Finally, the condition with the added social support had a significantly higher overall effectiveness gain than the condition with no added social support. The practical and theoretical implications of the results are discussed

Cytotoxic G-rich oligodeoxynucleotides: putative protein targets and required sequence motif

It has recently been shown that certain oligodeoxynucleotides (ODNs) designed as catalytic DNA molecules (DNAzymes) exhibit potent cytotoxicity independent of RNA-cleavage activity in a number of cell lines. These cytotoxic ODNs all featured a 5′ G-rich sequence and induced cell death by a TLR9-independent mechanism. In this study, we examined the sequence and length dependence of ODNs for cytotoxicity. A G-rich sequence at the 5′ terminus of the molecule was necessary for cytotoxicity and the potency of ODNs with active 5′ sequences was length dependent. Cytotoxicity appeared to be generally independent of 3′ sequence composition, although 3′ sequences totally lacking G-nucleotides were mostly inactive. Nucleolin, elongation factor 1-alpha (eEF1A) and vimentin were identified as binding to a cytotoxic ODN (Dz13) using protein pull-down assays and LC-MS/MS. Although these proteins have previously been described to bind G-rich ODNs, the binding of eEF1A correlated with cytotoxicity, whereas binding of nucleolin and vimentin did not. Quiescent non-proliferating cells were resistant to cytotoxicity, indicating cytotoxicity may be cell cycle dependent. Although the exact mechanism of cytotoxicity remains unknown, marked potency of the longer (⩾25 nt) ODNs in particular, indicates the potential of these molecules for treatment of diseases associated with abnormal cell proliferation

Early development of infants with neurofibromatosis type 1: a case series

Background Prospective studies of infants at familial risk for autism spectrum disorder (ASD) have yielded insights into the earliest signs of the disorder but represent heterogeneous samples of unclear aetiology. Complementing this approach by studying cohorts of infants with monogenic syndromes associated with high rates of ASD offers the opportunity to elucidate the factors that lead to ASD. Methods We present the first report from a prospective study of ten 10-month-old infants with neurofibromatosis type 1 (NF1), a monogenic disorder with high prevalence of ASD or ASD symptomatology. We compared data from infants with NF1 to a large cohort of infants at familial risk for ASD, separated by outcome at age 3 of ASD (n = 34), atypical development (n = 44), or typical development (n = 89), and low-risk controls (n = 75). Domains assessed at 10 months by parent report and examiner observation include cognitive and adaptive function, sensory processing, social engagement, and temperament. Results Infants with NF1 showed striking impairments in motor functioning relative to low-risk infants; this pattern was seen in infants with later ASD from the familial cohort (HR-ASD). Both infants with NF1 and the HR-ASD group showed communication delays relative to low-risk infants. Conclusions Ten-month-old infants with NF1 show a range of developmental difficulties that were particularly striking in motor and communication domains. As with HR-ASD infants, social skills at this age were not notably impaired. This is some of the first information on early neurodevelopment in NF1. Strong inferences are limited by the sample size, but the findings suggest implications for early comparative developmental science and highlight motor functioning as an important domain to inform the development of relevant animal models. The findings have clinical implications in indicating an important focus for early surveillance and remediation in this early diagnosed genetic disorder

Effects of a high-dose 24-h infusion of tranexamic acid on death and thromboembolic events in patients with acute gastrointestinal bleeding (HALT-IT): an international randomised, double-blind, placebo-controlled trial

Background: Tranexamic acid reduces surgical bleeding and reduces death due to bleeding in patients with trauma. Meta-analyses of small trials show that tranexamic acid might decrease deaths from gastrointestinal bleeding. We aimed to assess the effects of tranexamic acid in patients with gastrointestinal bleeding. Methods: We did an international, multicentre, randomised, placebo-controlled trial in 164 hospitals in 15 countries. Patients were enrolled if the responsible clinician was uncertain whether to use tranexamic acid, were aged above the minimum age considered an adult in their country (either aged 16 years and older or aged 18 years and older), and had significant (defined as at risk of bleeding to death) upper or lower gastrointestinal bleeding. Patients were randomly assigned by selection of a numbered treatment pack from a box containing eight packs that were identical apart from the pack number. Patients received either a loading dose of 1 g tranexamic acid, which was added to 100 mL infusion bag of 0·9% sodium chloride and infused by slow intravenous injection over 10 min, followed by a maintenance dose of 3 g tranexamic acid added to 1 L of any isotonic intravenous solution and infused at 125 mg/h for 24 h, or placebo (sodium chloride 0·9%). Patients, caregivers, and those assessing outcomes were masked to allocation. The primary outcome was death due to bleeding within 5 days of randomisation; analysis excluded patients who received neither dose of the allocated treatment and those for whom outcome data on death were unavailable. This trial was registered with Current Controlled Trials, ISRCTN11225767, and ClinicalTrials.gov, NCT01658124. Findings: Between July 4, 2013, and June 21, 2019, we randomly allocated 12 009 patients to receive tranexamic acid (5994, 49·9%) or matching placebo (6015, 50·1%), of whom 11 952 (99·5%) received the first dose of the allocated treatment. Death due to bleeding within 5 days of randomisation occurred in 222 (4%) of 5956 patients in the tranexamic acid group and in 226 (4%) of 5981 patients in the placebo group (risk ratio [RR] 0·99, 95% CI 0·82–1·18). Arterial thromboembolic events (myocardial infarction or stroke) were similar in the tranexamic acid group and placebo group (42 [0·7%] of 5952 vs 46 [0·8%] of 5977; 0·92; 0·60 to 1·39). Venous thromboembolic events (deep vein thrombosis or pulmonary embolism) were higher in tranexamic acid group than in the placebo group (48 [0·8%] of 5952 vs 26 [0·4%] of 5977; RR 1·85; 95% CI 1·15 to 2·98). Interpretation: We found that tranexamic acid did not reduce death from gastrointestinal bleeding. On the basis of our results, tranexamic acid should not be used for the treatment of gastrointestinal bleeding outside the context of a randomised trial

A loneliness that can be rocked : maternal loss, language, and unconventional mothering in contemporary African American literature.

Thesis (Ph. D.)--University of Rochester. Department of English, 2016.Motherhood and loss are inseparable in the African American literary canon. This connection extends as far back as Frederick Douglass and Harriet Jacobs' early slave narratives, where both cite the deaths of their mothers as a moment of personal and social disorientation. 20th and 21st century African American fiction responds to these prevailing representations of maternal loss, articulating the pain and loneliness that surviving children feel after the death of their biological mothers and the way in which other mothers--typically female relatives or friends--step in and assume child-rearing responsibilities. However, in the novels of Jamaica Kincaid, Jesmyn Ward, Toni Morrison, and Ayana Mathis, the death of the biological mother results in the emergence of unconventional mother figures. My dissertation sees these alternative narratives of mothering in contemporary African American literature as a means to articulate agency. Specifically, these narratives articulate a shift from motherhood, the institution, to mothering, a practice or experience. While this move from the institution of motherhood to the practice of mothering is applicable and vital to understanding the relationship between mothers and children in all communities, African American literature most effectively illustrates this shift because it so prominently features maternal loss or absence, which resulted from slavery and its aftermath. In Kincaid's The Autobiography of My Mother, Ward's Salvage the Bones, Morrison's Home, and Mathis' The Twelve Tribes of Hattie, motherhood is premised around storytelling and cultural transmission (important facets of African American maternity), rather than biological reproduction. As such, the novels' nontraditional mothers challenge the systemic inequalities reflected in the prevailing, dominant discourse that surrounds black maternity, as these mothers reject the social and political hierarchies and ideology that have supported the institutionalized degradation of black motherhood. Moreover, the novels, themselves, perform a type of unconventional mothering — akin to the mothering through cultural transmission depicted within their pages--as they use storytelling as a means for passing on African American history, culture, and values. These unconventional mothers are not meant to replace the biological mother or be hierarchized; instead, they illustrate the necessity of the mother--whether biological or unconventional--in African American literature and communities

Genomic Structure of the Salmonella enterica Serovar Typhimurium DT 64 Bacteriophage ST64T: Evidence for Modular Genetic Architecture

The complete sequence of the double-stranded DNA genome of a serotype-converting temperate bacteriophage, ST64T, was determined. The 40,679-bp genomic sequence of ST64T has an overall GC content of 47.5% and was reminiscent of a number of lambdoid phages, in particular, P22. Inferred proteins of ST64T which exhibited a high degree of sequence similarity to P22 proteins (>90%) included the functional serotype conversion cassette, integrase, excisionase, Abc1, Abc2, early antitermination (gp24), NinD, NinH, NinZ, packaging (gp3 and gp2), head (with the exception of gp26, gp7, gp20, and gp16), and tail proteins. The putative immunity genes were highly related to those of Salmonella enterica serotype Typhimurium phage L, whereas the lysis genes were almost identical to those of S. enterica serovar Typhimurium PS3

Cytotoxic G-rich oligodeoxynucleotides: putative

protein targets and required sequence moti

Comparison of Body Composition Measurements in Lean Female Athletes

International Journal of Exercise Science 11(4): 417-424, 2018. This study compared body composition measurements in lean female athletes. The primary objective was to compare the accuracy of percent body fat (%BF) determined by bioelectrical impedance analysis (BIA), air-displacement plethysmography (ADP), and underwater weighing (UWW) in female Division I cheerleaders (n = 10 bases, 6 back-spots, and 12 flyers) from two universities. The secondary objective was to compare health risk predicted by %BF to body mass index (BMI) categorizations. UWW was considered the gold standard for assessing %BF. Pearson correlation coefficients were used to determine associations between methods. Repeated measures analysis of variance was used to identify differences in %BF by method. BIA, ADP, and UWW were highly correlated (r ≥ .828, p \u3c .001 for all). However, %BF by BIA (20.0 ± 5.2%) and ADP (19.3 ± 6.0%) was higher than %BF by UWW (15.9 ± 4.1%, p \u3c .001). Health risk was predicted less often when classified based on very lean (risky low) %BF levels by BIA and ADP than UWW (7.1%, 3.6%, and 21.4%, respectively). This finding suggests that, similar to female track-and-field athletes who also exhibit lean muscular physiques, %BF is overestimated by BIA and ADP in female cheerleaders and health risk associated with low %BF is underestimated when compared to UWW. In contrast, BMI was not associated with %BF by any method and no participants were classified as underweight by this measure. Thus, BMI should not be used to predict health risk in lean female athletes, such as collegiate cheerleaders