Vislumbrando uma possibilidade para o envelhecimento ativo: as atividades universitárias para idosos

Objetivo: Desvelar as condições que permitem à Universidade Aberta à Terceira Idade configurar-se como possibilidade para o envelhecimento ativo. Método: Estudo qualitativo ancorado no referencial teórico do Interacionismo Simbólico e metodológico da Teoria Fundamentada nos Dados. Participaram, no período de abril a outubro de 2020, por meio de entrevistas individuais, 14 idosos, dois coordenadores e seis docentes vinculados às atividades universitárias para idosos. A análise dos dados foi realizada por três etapas interdependentes de codificações - aberta, axial e integração - com apoio do software Atlas.ti. Resultados: As condições que permitem ao programa configurar-se como oportunidade para o envelhecimento ativo relacionam-se aos significados atribuídos sobre envelhecer bem; à determinação social da participação; às motivações na busca pelo programa; às funções executadas pelo programa e; às políticas públicas e institucionais da universidade a qual se vincula. Considerações Finais: As atividades universitárias para idosos são uma possibilidade para o envelhecimento ativo, mas barreiras socialmente construídas dificultam o acesso às mesmas. Palavras-chave: Envelhecimento saudável. Universidades. Aposentadoria

HIV Infection among Illegal Migrants, Italy, 2004–2007

To determine HIV prevalence and place of exposure for illegal migrants in Italy, we tested 3,003 illegal adult migrants for HIV; 29 (0.97%) were HIV positive. Antibody avidity index results (indicators of time of infection) were available for 27 of those persons and showed that 6 (22.2%) presumably acquired their infection after migration

The response to postnatal stress: Amino acids transporters and pkc activity

During postnatal development, the central nervous system (CNS) is highly sensitive to effects of drugs, stressors and environment. In this study, we evaluated the effect of acute cold stress on g-aminobutyric acid (GABA) and L-serine (L-Ser) transporters in vitro, using the uptake of [3H]GABA and [3H]L-Ser by synaptosomes-enriched fractions isolated from rat cerebral cortex during postnatal development. We found that the maximal velocity (Vmax) L-Ser uptake after stress session was increased in almost all ages studied, except at postnatal-days (PD) 5, whereas the values of Km (uptake affinity) decreased in all ages from 5 to 21 PD compared with the control group. The highest values of Vmax and Km were obtained at PD7. At the same time, in GABA uptake, the Km decreased although Vmax increased in all ages considered after cold stress during development. Finally, we investigated the mechanism by which cells regulate the substrate affinity of L-serine and GABA transporters. We demonstrated a significantly increase in PKC activity to PD5 from PD21. Pretreatment with PKC inhibitor: staurosporine (SP) led to a restoration of control uptake in several postnatal-days suggesting a relationship between amino acids uptake and PKC activation. These findings suggest that a single exposure to postnatal cold stress at different periods after birth modifies both GABA and L-Ser transporters and the related increase in PKC activity could be intracellular events that participate in neuronal plasticity by early-life stress, which could be relevant to function of transporters in the adult rat brain.Fil: Odeon, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Salatino, Adrián Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Rodríguez, Carla Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Scolari, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Acosta, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentin

Apprendre à développer sa posture professionnelle

«Méthode, démarche et outils»: ce module clé a été mis en place par la Haute Ecole de Santé Vaud (HESAV) pour les finalistes de Bachelor en soins infirmiers. La formation privilégie l’approche de la classe inversée, qui favorise la réflexion alimentée par l’expérience personnelle ainsi que l’appropriation des contenus

Yeasts and Listeria monocytogenes Inactivation After Ultra High Pressure Treatments of Smoothies at Various Temperatures

Common microbial contaminants of fruit are yeasts and moulds; however, the thermoduric and psychrotrophic traits of Listeria monocytogenes make its presence in smoothies a microbiological risk also at refrigeration temperatures. Non-thermal pasteurization methods as High Hydrostatic Pressure (HHP) could fulfil the need of retaining smoothies freshness and healthy properties. In the present study, combined treatments of HHP (100 to 300 MPa) and temperature (-5\ub0C to 50\ub0C) were performed to inactivate mixed strains of Pichia ssp. and L. monocytogenes added to commercial smoothies (108 CFU ml-1). An inactivation model was developed according to a CCD. Counts of survivors by selective (SM), as well as recovery (RM) media, permitted to estimate the occurrence of Viable but Not Culturable (VNC) cells. The response surfaces showed a synergistic effect of T and HHP, giving the highest cell lethality at 300MPa either at 50\ub0C or -5\ub0C (>8 and 5.0 Log CFU ml-1 for Yeasts, 6.0 and 4.5 Log ml-1 for L. monocytogenes respectively); interestingly, the model showed a reduction of the treatment effectiveness (to < 1 Log CFU ml-1) as the T approaches the optimum growth temperature of the target species, regardless the HHP. Also, SM and RM counts indicated that VNC cells reached their maximum level at these temperatures for all the HHP employed

Vislumbrando uma possibilidade para o envelhecimento ativo: as atividades universitárias para idosos

Objetivo: Desvelar as condições que permitem à Universidade Aberta à Terceira Idade configurar-se como possibilidade para o envelhecimento ativo. Método: Estudo qualitativo ancorado no referencial teórico do Interacionismo Simbólico e metodológico da Teoria Fundamentada nos Dados. Participaram, no período de abril a outubro de 2020, por meio de entrevistas individuais, 14 idosos, dois coordenadores e seis docentes vinculados às atividades universitárias para idosos. A análise dos dados foi realizada por três etapas interdependentes de codificações - aberta, axial e integração - com apoio do software Atlas.ti. Resultados: As condições que permitem ao programa configurar-se como oportunidade para o envelhecimento ativo relacionam-se aos significados atribuídos sobre envelhecer bem; à determinação social da participação; às motivações na busca pelo programa; às funções executadas pelo programa e; às políticas públicas e institucionais da universidade a qual se vincula. Considerações Finais: As atividades universitárias para idosos são uma possibilidade para o envelhecimento ativo, mas barreiras socialmente construídas dificultam o acesso às mesmas. Palavras-chave: Envelhecimento saudável. Universidades. Aposentadoria

Mefloquine versus Quinine plus Sulphalene-Pyrimethamine (Metakelfin) for Treatment of Uncomplicated Imported Falciparum Malaria Acquired in Africa

We conducted a multicenter, randomized, open-label trial to compare mefloquine with a 3-day quinine plus sulphalene-pyrimethamine (SP) regimen for the treatment of imported uncomplicated malaria acquired in Africa. The end points of the study were efficacy, tolerability, and length of hospital stay. From July 1999 to February 2003, 187 patients were enrolled in five centers in Italy, of whom 93 were randomized to receive mefloquine (the M group) and 94 were randomized to receive quinine plus SP (the QSP group). Immigrants and visiting relatives and friends represented 90% of the cases and were mainly from western African countries. A slightly increased proportion of cases in the QSP group had abnormal alanine aminotransferase levels at the baseline. The early cure rate was similar in the two groups: 98.9% (confidence interval [CI] = 97 to 100%) in the M group and 96.8% (CI = 93 to 100%) in the QSP group. The extended follow-up was completed by 135 subjects (72.2%), and no case of recrudescence was detected. There were no differences in the parasite clearance time, but patients in the M group had shorter mean fever clearance time (35.9 h versus 44.4 h for the QSP group; P = 0.05) and a shorter mean hospital stay (3.9 days versus 4.6 days for the QSP group; P = 0.007). The overall proportions of reported side effects were similar in the two groups, but patients in the M group had a significantly higher rate of central nervous system disturbances (29.0% versus 9.6% for the QSP group; P < 0.001)

The coexistence center for elderly people and its importance in the support to the family and the Health Care Network

ABSTRACT Objective: To know the perception of relatives of elderly people about the coexistence center and its importance in supporting the Health Care Network. Methodology: A qualitative study carried out with 14 relatives of elderly people participating in a coexistence center. Data collection took place in January 2016 through individual interviews, which were recorded, transcribed and submitted to content analysis. The results were discussed in the light of the theoretical referential of health promotion. Results: The elderly's participation in the coexistence center was an alternative to support care and institutionalization, provided time for self-care and to maintain or engage in the formal labor market and positively influenced the family relationships. Conclusion: The coexistence center was set up as a health promotion institution, being jointly responsible for the care of the elderly with the Health Care Network and the families

Efecto de Mycoplasma y del agente de eliminación de micoplasmas en la actividad de las hidrolasas en fibroblastos de pacientes con enfermedades lisosomales

Este estudio fue disenado para evaluar el efecto de la contaminacion por micoplasmas sobre la actividad de hidrolasas acidas y la accion del agente de eliminacion de micoplasmas (MRA) en cultivos de fibroblastos humanos de pacientes con enfermedades lisosomales. Se midio la actividad de la b-galactosidasa, arilsulfatasa B (ASB), hexosaminidasa A y a-glucosidasa en estos cultivos. La actividad de estas enzimas en los fibroblastos contaminados por micoplasmas se midio antes y despues de la adicion de MRA. Los resultados se compararon con los obtenidos en cultivos libres de contaminacion. Solo la enzima ASB demostro alteracion significativa en la actividad, tanto en presencia de micoplasmas como con la adicion de MRA. Las enzimas restantes no sufrieron alteraciones significativas en presencia de micoplasmas, ni tras la adicion de MRA. La actividad medida para la enzima ASB aumento significativamente en presencia de micoplasmas y MRA, lo que podria conducir a un diagnostico dudoso. Por lo tanto, sugerimos evitar la contaminacion con micoplasmas mediante el uso de tecnicas asepticas y la utilizacion de MRA en los cultivos de fibroblastos que no se puedan descartar.This study was designed to evaluate the effect of mycoplasma contamination on acid hydrolase activity and the action of the mycoplasma removal agent (MRA), in cultures of human fibroblasts from individuals with lysosomal diseases. For this purpose, we measured the activity of the b-galactosidase, arylsulphatase B (ASB), hexosaminidase A and a-glucosidase enzymes. The activity of the above mentioned enzymes in fibroblasts contaminated by mycoplasma was measured before and after the addition of the MRA. The results were then compared to the enzymatic activity in contamination-free cultures. Only the ASB enzyme showed significant alteration in activity both in the presence of mycoplasma and MRA. The remaining enzymes did not suffer significant interference by the presence of the two agents. Of the four enzymes tested, three did not suffer significant alterations by the presence of the mycoplasma nor from the MRA. However, the activity measured in the ASB enzyme increased significantly in the presence of mycoplasma and MRA and could lead to a doubtful diagnosis. Therefore, we suggest that contamination should be prevented by using aseptic techniques as well as the MRA in those fibroblast cultures that cannot be discarded