Estimation of genetic parameters for height using spatial analysis in Tsuga heterophylla full-sibling family trials in British Columbia

Non-spatial and spatial analyses were carried out to study the effects on genetic parameters in ten-year height growth data across two series of 10 large second-generation full-sib progeny trials of western hemlock [Tsuga heterophylla (Raf.) Sarg.] in British Columbia. To account for different and complex patterns of environmental heterogeneity, spatial single trial analyses were conducted using an individual-tree mixed model with a two-dimensional smoothing surface with tensor product of B-spline bases. The spatial single trial analysis, in all cases, showed sizeable lower Deviance Information Criterion values relative to the non-spatial analysis. Also, fitting a surface displayed a consistent reduction in the posterior mean as well as a decrease in the standard deviations of error variance, no appreciable changes in the additive variance, an increase of individual narrow-sense heritability, and accuracy of breeding values. The tensor product of cubic basis functions of B-spline based on a mixed model framework does provide a useful new alternative to model different and complex patterns of spatial variability within sites in forest genetic trials. Individual narrow-sense heritabilities estimates from the spatial single trial analyses were low (average of 0.06), but typical of this species. Estimated dominance relative to additive variances were unstable across sites (from 0.00 to 1.59). The implications of these estimations will be discussed with respect to the western hemlock genetic improvement program in British Columbia.Fil: Cappa, Eduardo Pablo. British Columbia Ministry of Forests and Range; Canadá. University of British Columbia; Canadá. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yanchuk, A. D.. British Columbia Ministry of Forests and Range; CanadáFil: Cartwright, C. V.. British Columbia Ministry of Forests and Range; Canad

Upper body balance control strategy during continuous 3D postural perturbation in young adults

We explored how changes in vision and perturbation frequency impacted upright postural control in healthy adults exposed to continuous multiaxial support-surface perturbation. Ten subjects were asked to maintain equilibrium in standing stance with eyes open (EO) and eyes closed (EC) during sinusoidal 3D rotations at 0.25 (L) and 0.50 Hz (H). We measured upper-body kinematics – head, trunk, and pelvis – and analyzed differences in horizontal displacements and roll, pitch, and yaw sways. The presence of vision significantly decreased upper-body displacements in the horizontal plane, especially at the head level, while in EC the head was the most unstable segment. H trials produced a greater segment stabilization compared to L ones in EO and EC. Analysis of sways showed that in EO participants stabilized their posture by reducing the variability of trunk angles; in H trials a sway decrease for the examined segments was observed in the yaw plane and, for the pelvis only, in the pitch plane. Our results suggest that, during continuous multiaxial perturbations, visual information induced: (i) in L condition, a continuous reconfiguration of multi-body-segments orientation to follow the perturbation; (ii) in H condition, a compensation for the ongoing perturbation. These findings were not confirmed in EC where the same strategy – that is, the use of the pelvis as a reference frame for the body balance was adopted both in L and H

Walking the tightrope: Circular economy breadth and firm economic performance

The circular economy (CE) can bring benefits but also pitfalls to the production processes, affecting a firm's economic performance. Using data from European SMEs, we empirically investigate, from the perspective of self-determination theory, the extent to which the breadth of CE activities, that is, the number of CE activities undertaken by a firm, affects a firm's economic performance. Our study theorizes and shows that there is an inverted U-shaped effect brought about by the number of CE activities on economic performance. This research advances our scientific understanding of the CE and provides managers with suggestions on how to maximize the benefits generated by the CE in terms of economic performance by implementing the right amount of CE activities

870 micron continuum observations of the bubble-shaped nebula Gum 31

We are presenting here a study of the cold dust in the infrared ring nebula Gum 31. We aim at deriving the physical properties of the molecular gas and dust associated with the nebula, and investigating its correlation with the star formation in the region, that was probably triggered by the expansion of the ionization front. We use 870 micron data obtained with LABOCA to map the dust emission. The obtained LABOCA image was compared to archival IR,radio continuum, and optical images. The 870 micron emission follows the 8 micron (Spitzer), 250 micron, and 500 micron (Herschel) emission distributions showing the classical morphology of a spherical shell. We use the 870 micron and 250 micron images to identify 60 dust clumps in the collected layers of molecular gas using the Gaussclumps algorithm. The clumps have effective deconvolved radii between 0.16 pc and 1.35 pc, masses between 70 Mo and 2800 Mo, and volume densities between 1.1x10^3 cm^-3 and 2.04x10^5 cm^-3. The total mass of the clumps is 37600 Mo. The dust temperature of the clumps is in the range from 21 K to 32 K, while inside the HII region reaches ~ 40 K. The clump mass distribution is well-fitted by a power law dN/dlog(M/Mo) proportional to M^(-alpha), with alpha=0.93+/-0.28. The slope differs from those obtained for the stellar IMF in the solar neighborhood, suggesting that the clumps are not direct progenitors of single stars/protostars. The mass-radius relationship for the 41 clumps detected in the 870 microns emission shows that only 37% of them lie in or above the high-mass star formation threshold, most of them having candidate YSOs projected inside. A comparison of the dynamical age of the HII region with the fragmentation time, allowed us to conclude that the collect and collapse mechanism may be important for the star formation at the edge of Gum 31, although other processes may also be acting.Comment: 15 pages, 10 figures. Accepted for publication in A&

Molecular gas and star formation towards the IR dust bubble S24 and its environs

We present a multi-wavelength analysis of the infrared dust bubble S24, and its environs, with the aim of investigating the characteristics of the molecular gas and the interstellar dust linked to them, and analyzing the evolutionary status of the young stellar objects (YSOs) identified there. Using APEX data, we mapped the molecular emission in the CO(2-1), $^{13}$CO(2-1), C$^{18}$O(2-1), and $^{13}$CO(3-2) lines in a region of about 5'x 5' in size around the bubble. The cold dust distribution was analyzed using ATLASGAL and Herschel images. Complementary IR and radio data were also used.The molecular gas linked to the S24 bubble, G341.220-0.213, and G341.217-0.237 has velocities between -48.0 km sec$^{-1}$ and -40.0 km sec$^{-1}$. The gas distribution reveals a shell-like molecular structure of $\sim$0.8 pc in radius bordering the bubble. A cold dust counterpart of the shell is detected in the LABOCA and Herschel images.The presence of extended emission at 24 $\mu$m and radio continuum emission inside the bubble indicates that the bubble is a compact HII region. Part of the molecular gas bordering S24 coincides with the extended infrared dust cloud SDC341.194-0.221. A cold molecular clump is present at the interface between S24 and G341.217-0.237. As regards G341.220-0.213, the presence of an arc-like molecular structure at the northern and eastern sections of this IR source indicates that G341.220-0.213 is interacting with the molecular gas. Several YSO candidates are found to be linked to the IR extended sources, thus confirming their nature as active star-forming regions. The total gas mass in the region and the H$_2$ ambient density amount to 10300 M$_{\odot}$ and 5900 cm$^{-3}$, indicating that G341.220-0.213, G341.217-0.237, and the S24 HII region are evolving in a high density medium. A triggering star formation scenario is also investigated.Comment: 17 pages, 16 figures. Submitted to A&A. Revised according to the referee repor

Bayesian inference for multi-environment spatial individual-tree models with additive and full-sib family genetic effects for large forest genetic trials

Context: The gain in accuracy of breeding values with the use of single trial spatial analysis is well known in forestry. However, spatial analyses methodology for single forest genetic trials must be adapted for use with combined analyses of forest genetic trials across sites. Aims: This paper extends a methodology for spatial analysis of single forest genetic trial to a multi-environment trial (MET) setting. Methods: A two-stage spatial MET approach using an individual-tree model with additive and full-sib family genetic effects was developed. Dispersion parameters were estimated using Bayesian techniques via Gibbs sampling. The procedure is illustrated using height growth data at age 10 from eight large Tsuga heterophylla (Raf.) Sarg. second-generation full-sib progeny trials from two series established across seven sites in British Columbia (Canada) and on one in Washington (USA). Results: The proposed multi-environment spatial mixed model displayed a consistent reduction of the posterior mean and an increase in the precision of error variances than the model with Sets in Replicates or incomplete block alpha designs. Also, the multi-environment spatial model provided an average increase in the posterior means of the narrow- and broad-sense individual-tree heritabilities (h2N and h2B, respectively). No consistent changes were observed in the posterior means of additive genetic correlations (rAjj'). Conclusion: Although computationally demanding, all dispersion parameters were successfully estimated from the proposed multi-environment spatial individual-tree model using Bayesian techniques via Gibbs sampling. The proposed two-stage spatial MET approach produced better results than the commonly used non-spatial MET analysis.Fil: Cappa, Eduardo Pablo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yanchuk, Alvin D.. British Columbia Forest Service; CanadáFil: Cartwright, Charlie V.. British Columbia Forest Service; Canad

Concurrent repeatability and reproducibility analyses of four marker placement protocols for the foot-ankle complex

Multi-segment models of the foot have been proposed in the past years to overcome limitations imposed by oversimplified traditional approaches used to describe foot kinematics, but they have been only partially validated and never compared. This paper presents a unique comparative assessment of the four most widely adopted foot kinematic models and aims to provide a guidance for the clinical interpretation of their results. Sensitivity of the models to differences between treadmill and overground walking was tested in nine young healthy adults using a 1D paired t-test. Repeatability was assessed by investigating the joint kinematics obtained when the same operator placed the markers on thirteen young healthy adults in two occasions. Reproducibility was then assessed using data from three randomly selected participants, asking three operators to repeat the marker placement three times. The analyses were performed on sagittal kinematics using curve similarity and correlation indices (Linear Fit Method) and absolute differences between selected points. Differences between treadmill and overground gait were highlighted by all the investigated models. The two most repeatable and reproducible investigated models had average correlations higher than 0.70, with the lowest values (0.56) obtained for the midfoot. Averaged correlations were always higher than 0.74 for the former and 0.70 for the latter, with the lowest obtained for the midfoot (0.64 and 0.51). For all investigated models, foot kinematics generally showed low repeatability: normative bands must be adopted with caution when used for comparison with patient data

Short communication: Characterization of Staphylococcus aureus from bulk tank milk of dairy cattle in Lombardy (northern Italy).

ABSTRACT Staphylococcus aureus is one of the most important pathogens causing mastitis in cattle, and it is responsible for economic losses in dairy herds worldwide. The PCR amplification of the 16S-23S rRNA intergenic spacer (ribosomal spacer PCR, RS-PCR) allows a rapid classification of the strains in genotypes and genotypic clusters (CL), which are characterized by different epidemiological and clinical properties. Both RS-PCR and multi-locus sequence typing (MLST) were performed on strains isolated from bovine bulk tank milk (BTM) collected from dairy herds located in the Lombardy region (northern Italy), to outline the distribution of Staph. aureus genotypes in this geographical area. Out of 844 examined samples, 398 were positive for Staph. aureus, with a variable count (cfu/mL) Up to 8 colonies from each sample were genotyped. A total of 1,101 Staph. aureus strains were analyzed with RS-PCR, and only a selection of them (n = 86), in relation to their frequency and geographical origin, underwent MLST. This study revealed 8 major genotypic clusters (CLB, CLC, CLR, CLS, CLI, CLF, CLAO, and CLZ), of which Staph. aureus CLB (29.3%) was the most common. Samples of BTM positive for CLB had a Staph. aureus cfu/mL count significantly higher than the non-CLB positive ones. Our MLST analysis showed genotypes already known as bovine-associated in literature, such as clonal complexes CC8, CC97, and CC151. The same selection of 86 strains was also analyzed for the presence of the adlb gene, which was recently proposed as a possible marker of contagiousness. Most Staph. aureus belonging to CLB or CC8 carried the adlb gene (85%), whereas this gene was detected in only 9% of non-CLB strains (CLAA, CLBI, CLBJ, CLS). In conclusion, the present study confirms that Staph. aureus CLB, which is recognized as a contagious genotype, is a particularly relevant agent of intramammary infection in dairy cows in Lombardy, and indirectly supports the idea that adlb can be a possible marker of contagiousness of isolates

A submillimeter study of the IR dust bubble S 21 and its environs

Based on the molecular emission in the 12CO(2-1) and 13CO(2-1) lines, and on the continuum emission in the MIR and FIR towards the S 21 IR dust bubble, we analyze the physical characteristics of the gas and dust linked to the nebula and the presence of young stellar objects (YSOs) in its environs. The line emission reveals a clumpy molecular shell, 1.4 pc in radius, encircling S 21. The total molecular mass in the shell amounts to 2900 M⊙ and the original ambient density, 2.1 ×10 3 cm − 3 , indicating that the bubble is evolving in a high density interstellar medium. The image at 24 µm shows warm dust inside the bubble, while the emission in the range 250 to 870 µm reveals cold dust in its outskirts, coincident with the molecular gas. The detection of radio continuum emission indicates that the bubble is a compact Hii region. A search for YSOs using photometric criteria allowed to identify many candidates projected onto the molecular clumps. We analize if the collect and collapse process has triggered a new generation of stars.Basados en la emisión molecular en las líneas 12CO(2-1) y 13CO(2-1), y en la emisión en el continuo en el mediano y lejano infrarrojo hacia la burbuja S 21, analizamos las características físicas del gas y polvo asociado con S 21 y la presencia de objetos estelares jóvenes (YSOs) en su entorno. La emisión molecular revela una cáscara grumosa de 1.4 pc de radio rodeando a S 21. Su masa molecular es de 2900 M⊙ y la densidad ambiental original en la región, 2.1 ×10 3 cm − 3 , lo que indica que la burbuja evoluciona en un medio de alta densidad. La imagen a 24 µ m muestra polvo tibio dentro de la burbuja, mientras que la emisión en el rango 250 a 870 µm revela que hay polvo frío en la vecindad, coincidente con el gas molecular. La detección de emisión en el continuo de radio indica que S 21 es una región Hii compacta. Una búsqueda de YSOs utilizando criterios fotométricos permitió identificar muchos candidatos coincidentes con los grumos moleculares. Se analiza si el proceso de collect and collapse ha dado origen a una nueva generación de estrellas.Fil: Cappa, Cristina Elisabeth. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Duronea, Nicolas Urbano. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Vasquez, Javier. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Rubio, M.. Universidad de Chile. Facultad de Ciencias Fisicas y Matematicas; ChileFil: Firpo, V.. Universidad de la Serena; ChileFil: López Caraballo, C. H.. Universidad Católica de Chile; ChileFil: Borissova, J.. Universidad de Valparaiso; Chil