Draft genome sequence of Lactobacillus helveticus ATCC 12046

Lactobacillus helveticus is a lactic acid bacterium used traditionally in the dairy industry, especially in the manufacture of cheeses. We present here the 2,141,841-bp draft genome sequence of L. helveticus strain ATCC 12046, a potential starter strain for improving cheese production.Fil: Ruzal, Sandra Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Palomino, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fina Martin, Joaquina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Fernández Do Porto, Darío Augusto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Formación e Investigación en Enseñanza de las Ciencias; Argentin

Dexamethasone-Loaded Lipomers: Development, Characterization, and Skin Biodistribution Studies

Follicular targeting has gained more attention in recent decades, due to the possibility of obtaining a depot effect in topical administration and its potential as a tool to treat hair follicle-related diseases. Lipid core ethyl cellulose lipomers were developed and optimized, following which characterization of their physicochemical properties was carried out. Dexamethasone was encapsulated in the lipomers (size, 115 nm; polydispersity, 0.24; zeta-potential (Z-potential), +30 mV) and their in vitro release profiles against dexamethasone in solution were investigated by vertical diffusion Franz cells. The skin biodistribution of the fluorescent-loaded lipomers was observed using confocal microscopy, demonstrating the accumulation of both lipomers and fluorochromes in the hair follicles of pig skin. To confirm this fact, immunofluorescence of the dexamethasone-loaded lipomers was carried out in pig hair follicles. The anti-inflammatory (via TNFα) efficacy of the dexamethasone-loaded lipomers was demonstrated in vitro in an HEK001 human keratinocytes cell culture and the in vitro cytotoxicity of the nanoformulation was investigated

The physicochemical, biopharmaceutical, and in vitro efficacy properties of freeze-dried dexamethasone-loaded lipomers.

Dexamethasone-loaded polymer hybrid nanoparticles were developed as a potential tool to treat alopecia areata due to their follicular targeting ability. Freeze drying (FD) is a common technique used to improve nanoparticle stability; however, there are few studies focused on its effect on ethyl cellulose lipid-core nanoparticles. Nanoparticles were lyophilized with different cryoprotectants. Sucrose was selected because it allowed for a good resuspension and provided acceptable physicochemical parameters (374.33 nm, +34.7 mV, polydispersion 0.229%, and 98.87% encapsulation efficiency). The nanoparticles obtained were loaded into a pleasant xanthan gum hydrogel, and the rheological, release, and skin permeation profiles of different formulations were studied. The FD formulation significantly modified the particle size, and the drug release and permeation properties were also altered. In addition, analyses of the cytotoxicity and anti-inflammatory efficacy of FD and non-FD particles on human keratinocytes indicated no differences

ESTILOS DE CRIANZA PARENTAL Y ACTITUDES MATERNAS EN MADRES DEL NIVEL INICIAL, FERREÑAFE – 2017

Este reciente estudio tuvo como propósito conocer si existe relación entre los estilos de crianza de la madre con las actitudes maternas. La muestra estuvo constituida por 85 madres de familia de una I.E. Inicial de la provincia de Ferreñafe. La investigación fue conducida en el marco del enfoque cuantitativo y diseño transversal correlacional. Se utilizó la Escala de Actitudes Maternas de Robert Roth y, el Cuestionario de Afecto y el Cuestionario de Normas y Exigencias de Bersabè. Los resultados del objetivo general demuestran que las actitudes maternas si se relacionan con los estilos de crianza de los modelos teóricos utilizados, sin embargo, en los aspectos de las dimensiones se han encontrado relaciones qué se han discutido en la presente investigación sus implicancias teóricas. La actitud materna que más sobresale es la de Aceptación, y el estilo de crianza que predomina es el Rígido.Tesi

Latanoprost-loaded nanotransfersomes designed for scalp administration enhance keratinocytes proliferation.

Latanoprost (LAT) has been shown to have a hypertrichotic effect, which makes it a promising candidate for alopecia treatments. For the first time, LAT has been encapsulated in nanotransfersomes in order to increase its efficacy. Ex vivo skin biodistribution was studied by confocal laser microscopy both in human scalp and pig skin. Results showed that nanotransfersomes increase the penetration of two different fluorochromes, with similar patterns in both species, compared with fluorochrome solutions containing no nanotransfersomes. Nanotransfersomes were stable under accelerated conditions (40 °C/75% RH) and long-term conditions (25 °C/60% RH) for up to 1 year, with no differences in vesicle size and polydispersity when LAT was loaded. Nanotransfersomes increased the LAT cell proliferation effect in HaCaT cell via MAPK signaling pathway. Collectively, our results demonstrate LAT-nanotransfersomes formulation could be a promising therapy for hair growth disorders

From the sea to the table: the environmental impact assessment of fishing, processing, and end-of-life of albacore in Cantabria

Cantabria, a small coastal region of Northern Spain, is one of the biggest producers of gourmet tuna cans in Europe. The fish capture in the Cantabrian Sea and the subsequent transformation in a local processing plant give distinction to this product, which is widely marketed in cans of 105 g of net weight. This work evaluates for the first time the environmental profile of the whole supply chain of this product, from fishing, processing, and waste valorization to inter-stage transport and packaging management in the end-of-life. To this end, the life cycle assessment methodology was applied considering primary data from the stakeholders involved in the supply chain and analyzing the seven most studied categories in this sector. Results revealed that fishing and processing accounted for the majority of the environmental impacts, while valorization and end-of-life treatments only avoid less than 10% of the burdens. The most important findings are focused on the high dependence on fuel use, identified as a hotspot in most stages although low compared to other fisheries, and on the intensive use of resources, especially sunflower oil, which contributes more than 70% of the impact on the global warming potential of the processing. This current framework forces the enhancement of the efficiency of a sector that attempts to engage the challenge of societal sustainability, by identifying the critical points and guiding policy makers on the path to sustainable development.This work was supported by the EAPA_576/2018NEPTUNUS project. The authors would like to acknowledge the financial support of Interreg Atlantic Area. The authors are grateful for the funding of the Spanish Ministry of Science and Innovation through the KAIROS-BIOCIR project (PID2019-104925RB) (AEO/FEDER, UE). Ana Fernández thanks the Ministry of Economy and Competitiveness of Spanish Government for their financial support via the research fellowship RE2020-094029. We also want to thank the fishermen and those responsible for the processing plant for the provision of the data

The Treatment With the SGLT2 Inhibitor Empagliflozin Modifies the Hepatic Metabolome of Male Zucker Diabetic Fatty Rats Towards a Protective Profile

[Abstract] The EMPA-REG OUTCOME (Empagliflozin, Cardiovascular Outcome Event Trial in patients with Type 2 Diabetes Mellitus (T2DM)) trial evidenced the potential of sodium-glucose cotransporter 2 (SGLT2) inhibitors for the treatment of patients with diabetes and cardiovascular disease. Recent evidences have shown the benefits of the SGLT2 inhibitor empagliflozin on improving liver steatosis and fibrosis in patients with T2DM. Metabolomic studies have been shown to be very useful to improve the understanding of liver pathophysiology during the development and progression of metabolic hepatic diseases, and because the effects of empagliflozin and of other SGLT2 inhibitors on the complete metabolic profile of the liver has never been analysed before, we decided to study the impact on the liver of male Zucker diabetic fatty (ZDF) rats of a treatment for 6 weeks with empagliflozin using an untargeted metabolomics approach, with the purpose to help to clarify the benefits of the use of empagliflozin at hepatic level. We found that empagliflozin is able to change the hepatic lipidome towards a protective profile, through an increase of monounsaturated and polyunsaturated glycerides, phosphatidylcholines, phosphatidylethanolamines, lysophosphatidylinositols and lysophosphatidylcholines. Empagliflozin also induces a decrease in the levels of the markers of inflammation IL-6, chemerin and chemerin receptor in the liver. Our results provide new evidences regarding the molecular pathways through which empagliflozin could exert hepatoprotector beneficial effects in T2DM.This work was supported by Boehringer Ingelheim Pharma GmbH and Co., by the National Institute of Health “Fondo de Investigaciones Sanitarias del Instituto de Salud Carlos III” Madrid, Spain (PI15/00681, PI17/00409, PI18/00821, PI20/00902, RETICS Programme RD16/0012/0014 and CIBER de Enfermedades Cardiovasculares (CIBERCV)); European Regional Development Fund (FEDER) and European Union framework MSCA-RISE-H2020 Programme (Project number 734899). AH-A was funded by predoctoral research grants from Xunta de Galicia and FPU Program of the Spanish Ministry of Science, Innovation and Universities (Spain); MF-S was funded by the predoctoral research grants “Programa Científico do Centro de Investigación en Medicina Molecular e Enfermidades Crónicas (CiMUS) (Spain) and Xunta de Galicia; and AV-L was funded by the predoctoral research grant from the PFIS Program of the Spanish Ministry of Science and Instituto de Salud Carlos III (Spain

Quercetin ameliorates dysregulation of lipid metabolism genes via the PI3K/AKT pathway in a diet-induced mouse model of non-alcoholic fatty liver disease

P. 879-893Flavonoids and related compounds seem to have favorable effects on nonalcoholic fatty liver disease (NAFLD) progression, although the exact mechanisms implicated are poorly understood. In this study, we aimed to investigate the effect of the flanovol quercetin on gene expression deregulation involved in the development of NAFLD, as well as the possible implication of phosphatidylinositol 3-kinase (PI3K)/AKT pathway modulatio

La Imagen y la Narrativa como Herramientas para el Abordaje Psicosocial en Escenarios de Violencia. Departamento de Cundinamarca.

Durante décadas, Colombia ha padecido las inclemencias del conflicto armado, el accionar delictivo de grupos al margen de la ley, el narcotráfico, el inconformismo social y político, la confrontación de poderes, posiciones ideológicas y vulneración de derechos, trayendo consigo las víctimas del conflicto armado, y naturalizando acciones terroríficas como el desplazamiento, la desaparición forzada, las masacres, las amenazas, el reclutamiento, el hostigamiento militar, la estigmatización, entre otros. Algunos de éstos fenómenos, analizados en éste documento, desde la realidad que relatan las narrativas, de individuos y colectivos, víctimas del conflicto armado. Primeramente, se analiza el relato de Camilo, un joven que permite evidenciar las repercusiones de la violencia en un joven afrocolombiano. Se determinan emergentes psicosociales, voces subjetivas y posicionamiento resiliente, a partir de la narrativa de Camilo. De igual manera, se formulan 9 preguntas, que permiten indagar sobre el caso estudiado, ampliando conocimientos, posturas y realidades subjetivas. Posteriormente, se continúa con el análisis del caso de Peñas Coloradas, comunidad víctima del conflicto armado, del hostigamiento militar, del desplazamiento y el destierro indefinido de su territorio, condiciones que conllevan otras situaciones difíciles como la pobreza, el hacinamiento, las agresiones y disfunciones psicosociales. A partir de éste caso, se hace un análisis y se plantean estrategias de abordaje psicosocial, con el fin de apoyar a las víctimas, acompañar su proceso de reconstrucción social y mitigar el impacto que ha ocasionado el experimentar esta situación de violencia. Para finalizar, se exhibe el informe que da cuenta de la experiencia de aplicación de la foto voz, como herramienta de interpretación de escenarios permeados por diversas formas de violencia fundamentadas en proyecciones de memorias individuales y colectivas, que identifican las huellas en la memoria y la capacidad para reconstruir la dignidad y trasformar voces invisibilizadas.For decades, Colombia has suffered the harshness of the armed conflict, the criminal actions of groups outside the law, drug trafficking, social and political nonconformism, the confrontation of powers, ideological positions and violation of rights, bringing with them the victims of the armed conflict, and naturalizing terrifying actions such as displacement, forced disappearance, massacres, threats, recruitment, military harassment, stigmatization, among others. Some of these phenomena, analyzed in this document, from the reality that relate the narratives of individuals and groups, victims of the armed conflict. First, we analyze the story of Camilo, a young man who can show the repercussions of violence on a young Afro-Colombian. Emerging psychosocial, subjective voices and resilient positioning are determined, based on Camilo’s narrative. In the same way, 9 questions are asked, which allow to inquire about the case studied, expanding knowledge, positions and subjective realities. Subsequently, we continue with the analysis of the case of Peñas Coloradas, a community victim of armed conflict, military harassment, displacement and indefinite banishment from its territory, conditions that lead to other difficult situations such as poverty, Overcrowding, abuse and psychosocial dysfunction. Based on this case, an analysis is made and strategies are proposed for a psychosocial approach, in order to support the victims, accompany their process of social reconstruction and mitigate the impact caused by experiencing this situation of violence. To conclude, we present the report that gives an account of the experience of applying photo voice, as a tool for interpreting scenarios permeated by various forms of violence based on projections of individual and collective memories, identifying traces in memory and the ability to rebuild dignity and transform invisible voices