Semi-intensive production of Penaeus vannamei in Guatemala

SUMMARY Because marine shrimp species convenient for aquacultural purposes live mainly in warm-water environments, commercial shrimp farms are located principally along tropical and sub-tropical marine coastlines. Other than the South-East Asiatic countries, the Latin America countries are important shrimp producers that contribute in satisfying the international demand of marine shrimp. Part of the imported shrimp in Italy come from Guatemala, where the shrimp aquaculture (representing around the 88% of the total aquaculture production) is based mainly on the Pacific white shrimp (Penaeus vannamei). Common rearing procedures of one of the biggest marine shrimp farms operating in Guatemala are described. Some productive parameters of Penaeus vannamei brought out by semi-intensive system in earthen ponds are quantified. Productive data result in a uniform distribution of weight classes at different days after stocking, besides the growth trend and feed conversion ratio agree with the growth performance of Penaeus vannamei. Growth data, fitted on the von Bertalanffy model, present the maximum weight gain (0.13 g/day) 28 days after stocking. Shrimp survival after a grow-out period of 80 days in a 2.44-ha pond is around 56% and the gross yield turns out to be more than one ton per hectare. In conclusion, semi-intensive shrimp farming performed in Guatemala allows to have high productivities as well as a good quality product. On the other hand it must be highlighted that this kind of cultivation represents a risk for the environmental equilibrium along the coasts of tropical countries. RIASSUNTO Poiché le specie di gambero marino utilizzate a fini produttivi sono originarie principalmente degli ambienti con acqua a temperatura elevata, le aziende che producono questi gamberi sono situate maggiormente lungo le coste dei Paesi tropicali e sub-tropi- cali. Oltre ai Paesi del Sud-Est Asiatico, i Paesi dell’America Latina sono importanti produttori, che contribuiscono a soddisfare la domanda internazionale di gamberi marini. Parte dei gamberi importati in Italia proviene dal Guatemala, dove la produzione di gamberi, che rappresenta l’88% della produzione nazionale di acquacoltura, è basata principalmente sul gambero bianco del Pacifico (Penaeus vannamei). Vengono descritte le comuni procedure produttive di una delle più grandi aziende di gamberi marini operanti in Guatemala. Vengono inoltre valutati alcuni parametri produttivi del Penaeus vannamei, allevato con un sistema semi-intensivo all’interno dei bacini di questa azienda. I dati di peso risultano distribuiti uniformemente alle diverse epoche dopo la semina, inoltre l’andamento dei pesi e l’indice di conversione alimentare concordano con i parametri di crescita del Penaeus vannamei. I dati di crescita, stimati con il modello proposto da von Bertalanffy, evidenziano il massimo incremento di peso (0,13 g al giorno) al 28° giorno dalla semina. La sopravvivenza dei gamberi dopo un periodo di accrescimento di 80 giorni in un bacino di 2,44 ettari è di circa il 56% e la resa supera il quantitativo di una tonnellata per ettaro. Nel complesso l’allevamento di gamberi con sistema semiintensivo, condotto in Guatemala, sembra fornire un’alta produttività combinata con una buona qualità del prodotto finale. D’altronde deve essere considerato che questo sistema d’allevamento rappresenta un serio rischio per l’equilibrio ambientale lungo le coste dei paesi tropicali

Identification of an antigenic domain near the C terminus of human granulocyte-macrophage colony-stimulating factor and its spatial localization.

The goal of this study was to map an epitope on the human granulocyte-macrophage colony-stimulating factor (hGM-CSF) at its C terminus, a region whose integrity is fundamental in maintaining the normal function of this molecule. Residues including the fourth alpha-helix (D, 103-116) were analyzed for their role in the interaction with antibodies (Abs) raised against the protein. Five peptides homologous to different segments of the C terminus of hGM-CSF were synthesized. Peptide-(102-121) included the same residues of the alpha-helix D and the next five amino acids toward the C terminus; peptide-[E108A]-(102-121) introduced the mutation E108A in order to verify the role of acidic residues; peptide-[C96A](93-110) encompassed the beta-sheet 2 and half of the alpha-helix D; peptide-[C121A]-(110-127) included the second half of the alpha-helix D and the C terminus of hGMCSF; peptide-(13-31)-Gly-Pro-Gly-(103-116) included both the alpha-helices A and D connected by the tripeptide Gly-Pro-Gly, which allows the original antiparallel orientation of the two alpha-helices to be maintained. Both anti-protein and anti-peptide-(102-121) antibodies, capable of neutralizing the stimulatory activity of hGMCSF in the bone marrow colony-forming assays, recognized a specific epitope in the C terminus of hGM-CSF. Molecular modeling estimated the surface accessibility of hGM-CSF and the stability of the synthetic peptides in aqueous solution. Altogether, our results showed that the immunogenic region includes part of the alpha-helix D and the residues 116-120, which are external to this helix and particularly exposed on the protein surface, confirming the feasible participation of this region in antibody binding

Bilateral reconstruction of the mandibular body with symphyseal preservation using a single fibula free flap: operative technique

Mandibular osteonecrosis may occur in 5% of the patients who undergo radiotherapy for the treatment of head and neck malignancies. Resection and microvascular reconstruction is the treatment of choice in complicated osteoradionecrosis, however multifocal presentation may complicate the management of the disease given the poor quality and limited availability of adequate recipient vessels

Pulsed Electro-Magnetic Field (PEMF) Effect on Bone Healing in Animal Models: A Review of Its Efficacy Related to Different Type of Damage

Biophysical energies are a versatile tool to stimulate tissues by generating biopotentials. In particular, pulsed electromagnetic field (PEMF) stimulation has intrigued researchers since the 1970s. To date, many investigations have been carried out in vivo, but a gold standard treatment protocol has not yet been defined. The main obstacles are represented by the complex setting of PEMF characteristics, the variety of animal models (including direct and indirect bone damage) and the lack of a complete understanding of the molecular pathways involved. In the present review the main studies about PEMF stimulation in animal models with bone impairment were reviewed. PEMF signal characteristics were investigated, as well as their effect on molecular pathways and osseous morphological features. We believe that this review might be a useful starting point for a prospective study in a clinical setting. Consistent evidence from the literature suggests a potential beneficial role of PEMF in clinical practice. Nevertheless, the wide variability of selected parameters (frequency, duration, and amplitude) and the heterogeneity of applied protocols make it difficult to draw certain conclusions about PEMF effectiveness in clinical implementation to promote bone healing. Deepening the knowledge regarding the most consistent results reported in literature to date, we believe that this review may be a useful starting point to propose standardized experimental guidelines. This might provide a solid base for further controlled trials, to investigate PEMF efficacy in bone damage conditions during routine clinical practic

Primary Intraosseous Squamous Cell Carcinoma of the Anterior Mandible Arising in an Odontogenic Cyst in 34-Year-Old Male

Abstract: Primary intraosseous squamous cell carcinoma (PIOSCC) is a rare and aggressive malignancy arising exclusively within the jaws, without any initial connection with the oral mucosa. The etiology and the epidemiology are unclear due to the rarity of the disease, and there is no current universally accepted staging or treatment protocol. Clinically, the posterior mandible is the most a ected site, and common symptoms are swelling and pain. The diagnosis is often di cult either because it requires stringent criteria to be satisfied or because of the absence of a pathognomonic histological pattern. Aggressive surgery is the first-choice treatment, often followed by radiotherapy. The lymph nodal status seems to be the most important factor influencing the prognosis, which is usually poor, with a 5-year survival rate ranging from 30% to 40%. In the present article, we report an unusual case of cystogenic PIOSCC interesting the anterior mandible of a young 34-year-old male, which came to our attention after complaint about recurrent infective episodes a ecting a dentigerous cyst (impacted lower canine) discovered ten years before. The age, site, and extension are uncommon. Extensive surgical treatment with fibula free flap reconstruction, adjuvant therapy, and salvage surgery was carried out. The patient was disease-free at a 31-month follow-up

Bone Healing Evaluation Following Different Osteotomic Techniques in Animal Models: A Suitable Method for Clinical Insights

Osteotomy is a common step in oncological, reconstructive, and trauma surgery. Drilling and elevated temperature during osteotomy produce thermal osteonecrosis. Heat and associated mechanical damage during osteotomy can impair bone healing, with consequent failure of fracture fixation or dental implants. Several ex vivo studies on animal bone were recently focused on heating production during osteotomy with conventional drill and piezoelectric devices, particularly in endosseous dental implant sites. The current literature on bone drilling and osteotomic surface analysis is here reviewed and the dynamics of bone healing after osteotomy with traditional and piezoelectric devices are discussed. Moreover, the methodologies involved in the experimental osteotomy and clinical studies are compared, focusing on ex vivo and in vivo findings

Tongue cancer following hematopoietic cell transplantation for Fanconi anemia

Objectives: The aim of this retrospective study was to determine the incidence and the clinical outcome of tongue cancer (TC) in patients affected by Fanconi anemia (FA) who received an allogeneic hematopoietic cell transplantation (HCT). Materials and methods: The patient database from the Bone Marrow Transplant Center of Pescara was reviewed to enroll FA patients. Patients', donors', HCT's, and screening's data were collected as well to look for the incidence and the treatment of TC. Results: Twelve patients affected by FA were identified. Three patients died for transplant-related causes. Five of nine surviving patients were diagnosed with TC at a median of 21.7 years since transplantation and at a median age of 32.10 years. Interestingly, no patient manifested graft-versus-host-disease (GvHD). The 28-year cumulative incidence function of TC was 46.9% (95% CI, 36.9-56.9%). Two patients were treated with chemotherapy alone, two patients were treated with surgery alone, and one with surgery followed by chemotherapy. Overall, 4 patients with TC showed a clinical course characterized by a marked aggressiveness of the tumor disease which led to death due to cancer progression between 2 and 13 months. One patient is surviving 8 months after diagnosis of TC. Conclusions: Our study confirms the high incidence of tumors and in particular tongue tumors in allotransplanted FA patients. A careful screening has to be life-long maintained. Clinical relevance: Considering the rarity of FA and the frailty of FA patients, this study may add important information for the cancer management of these patients

Evidence for surface faulting earthquakes on the Montereale fault system (Abruzzi Apennines, central Italy)

We conducted paleoseismic studies along the Montereale fault system (MFS; central Italy). The MFS shows geomorphological evidence of Late Quaternary activity and falls within the highest seismic hazard zone of central Apennines, between the epicentral areas of two recent earthquake sequences: 2009 L’Aquila and 2016–2017 central Italy. We excavated two trenches along the San Giovanni fault splay of the system, one intercepting the N140° striking bedrock main fault plane and the other cutting two subparallel fault scarps on the colluvial/alluvial deposits on the fault hanging wall. Excavations revealed repeated fault reactivation with surface faulting in prehistorical and historical times. We recognized and dated seven events in the last 26 kyr. The most recent ground-rupturing event (evb1) possibly occurred 650–1,820 AD, consistent with one of the three main shocks that struck the area in 1,703 AD. A previous event (evb2) occurred between 5,330 BC and 730 BC, while older events occurred at 6,590–5,440 BC (evb3), 9,770–6,630 BC (evb4), and 16,860–13,480 BC (evb5). We documented two older displacement events (evb7 and evb6) between 23,780 BC and 16,850 BC. The minimum vertical slip rate at the trench site in the last 28–24 kyr is 0.3–0.4 mm/year. The inferred average recurrence interval for surface-faulting events along the MFS is no longer than ~4 kyr. Based on the surface fault length ranging between 12 and 20 km, earthquakes with ≥M 6.0 are possible for the MFS. The MFS is an independent earthquake source, and its paleoseismic data are fully comparable with those known for faults in central Apennines.Published2758-27766T. Studi di pericolosità sismica e da maremotoJCR Journa

Abnormal B-Cell Maturation and Increased Transitional B Cells in CBL Syndrome

CBL syndrome is a Noonan-like RASopathy with heterogeneous clinical phenotype and predisposition to juvenile myelomonocytic leukemia (JMML). Here we describe two patients with identical germline CBL mutation and clinical and immune-hematological overlapping features with autoimmune lymphoproliferative syndrome (ALPS) and B-cell expansion with NF-κB and T-cell anergy (BENTA) syndrome. Increased immature/transitional B cells can be depicted in CBL syndrome, ALPS, and BENTA. Nonetheless, our patients here described showed peculiar B-cell phenotype due to increased immature/transitional CD34+ B cells. This feature differentiates CBL syndrome from BENTA, pointing toward an abnormal proliferation of B-cell early precursors

The population of merging compact binaries inferred using gravitational waves through GWTC-3

We report on the population properties of 76 compact binary mergers detected with gravitational waves below a false alarm rate of 1 per year through GWTC-3. The catalog contains three classes of binary mergers: BBH, BNS, and NSBH mergers. We infer the BNS merger rate to be between 10 $\rm{Gpc^{-3} yr^{-1}}$ and 1700 $\rm{Gpc^{-3} yr^{-1}}$ and the NSBH merger rate to be between 7.8 $\rm{Gpc^{-3}\, yr^{-1}}$ and 140 $\rm{Gpc^{-3} yr^{-1}}$ , assuming a constant rate density versus comoving volume and taking the union of 90% credible intervals for methods used in this work. Accounting for the BBH merger rate to evolve with redshift, we find the BBH merger rate to be between 17.9 $\rm{Gpc^{-3}\, yr^{-1}}$ and 44 $\rm{Gpc^{-3}\, yr^{-1}}$ at a fiducial redshift (z=0.2). We obtain a broad neutron star mass distribution extending from $1.2^{+0.1}_{-0.2} M_\odot$ to $2.0^{+0.3}_{-0.3} M_\odot$. We can confidently identify a rapid decrease in merger rate versus component mass between neutron star-like masses and black-hole-like masses, but there is no evidence that the merger rate increases again before 10 $M_\odot$. We also find the BBH mass distribution has localized over- and under-densities relative to a power law distribution. While we continue to find the mass distribution of a binary's more massive component strongly decreases as a function of primary mass, we observe no evidence of a strongly suppressed merger rate above $\sim 60 M_\odot$. The rate of BBH mergers is observed to increase with redshift at a rate proportional to $(1+z)^{\kappa}$ with $\kappa = 2.9^{+1.7}_{-1.8}$ for $z\lesssim 1$. Observed black hole spins are small, with half of spin magnitudes below $\chi_i \simeq 0.25$. We observe evidence of negative aligned spins in the population, and an increase in spin magnitude for systems with more unequal mass ratio