Cyclative cleavage via solid-phase supported stabilized sulfur ylides: synthesis of macrocyclic lactones

A new synthesis of macrolactones bearing a cyclopropyl ring condensed to the macrocycle is reported via a cyclization-release strategy making use of solid-phase supported stabilized sulfur ylides

Real-time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS-540 mutations in Plasmodium falciparum

BACKGROUND: Sulphadoxine-pyrimethamine has been abandoned as first- or second-line treatment by most African malaria endemic countries in favour of artemisinin-based combination treatments, but the drug is still used as intermittent preventive treatment during pregnancy. However, resistance to sulphadoxine-pyrimethamine has been increasing in the past few years and, although the link between molecular markers and treatment failure has not been firmly established, at least for pregnant women, it is important to monitor such markers. METHODS: This paper reports a novel sensitive, semi-quantitative and specific real-time PCR and melting curve analysis (MCA) assay using fluorescence resonance energy transfer (FRET) for the detection of DHPS-540, an important predictor for SP resistance. FRET/MCA was evaluated using 78 clinical samples from malaria patients and compared to PCR-RFLP. RESULTS: Sixty-two samples were in perfect agreement between both assays. One sample showed a small wild type signal with FRET/MCA that indicates a polyclonal infection. Four samples were not able to generate enough material in both assays to distinguish mutant from wild-type infection, six samples gave no signal in PCR-RFLP and five samples gave no amplification in FRET/MCA. CONCLUSION: FRET/MCA is an effective tool for the identification of SNPs in drug studies and epidemiological surveys on resistance markers in general and DHPS-540 mutation in particular

City rankings and the citizens: exposing representational and participatory gaps

Acritical reflection on the purposes, role and performance of city rankings through an holistic communicational approach is at the core of this article. Grounded on a conceptual framework that highlights the contemporary idea of the city—beyond the smart city and more as a co-intelligent, collaborative and co-creative entity, and on the performance outputs of city rankings as territorial and strategic communication tools that actually represent the state of cities, we address the citizens’ presence or contribute—as main city stakeholders—to city rankings. In order to make research tangible with a practical component, an exploratory comparative content analysis of three recognized city rankings: the CBI – City Brands Index 2017, the GCR – 2018 Global Cities Report, and the Global Liveability Index 2018—was carried out. Conclusive notes argue that in order to effectively represent cities, as they are lived, thought and built by their citizens in their everyday, city rankings must rely in more real-time, updated, people’s perception centred data, and embed more citizen participation and insights. Moreover, methodology transparency and accountability should be promoted in order to add trust value to city rankings.(undefined

The ultraviolet flare at the center of the elliptical galaxy NGC 4278

A large fraction of otherwise normal galaxies shows a weak nuclear activity. One of the signatures of the low-luminosity active galactic nuclei (LLAGNs) is the ultraviolet variability which was serendipitously discovered in the center of some low-ionization nuclear emission-line region (LINER) galaxies. There is a pressing need to acquire better statistics about UV flaring and variability in galaxy nuclei, both in terms of the number and monitoring of targets. The Science Data Archive of the Hubble Space Telescope was queried to find all the elliptical galaxies with UV images obtained in different epochs with the Wide Field Planetary Camera 2 (WFPC2) and possibly with nuclear spectra obtained with the Space Telescope Imaging Spectrograph (STIS) in the region of the Halpha emission line. These data were found only for the elliptical radiogalaxy NGC 4278. The UV flux of the nuclear source of NGC 4278 was measured by means of aperture photometry on the WFPC2/F218W images obtained between June 1994 and January 1995. The mass of the central supermassive black hole (SBH) was estimated by measuring the broad components of the emission lines observed in the STIS/G750M spectrum and assuming that the gas is uniformly distributed in a sphere. The nucleus of NGC 4278 hosts a barely resolved but strongly variable UV source. Its UV luminosity increased by a factor of 1.6 in a period of 6 months. The amplitude and scale time of the UV flare in NGC 4278 are remarkably similar to those of the brightest UV nuclear transients which were earlier found in other LLAGNs. The mass of the SBH was found to be in the range between 7x10^7 and 2x10^9 M_sun. This is in agreement with previous findings based on different assumptions about the gas distribution and with the predictions based on the galaxy velocity dispersion.Comment: 5 pages,3 figures, accepted for publication on A&

Temporary techno-social gatherings? A (hacked) discussion about open practices

This paper is rooted in an experimental inquiry of issue-oriented temporary techno-social gatherings or TTGs, which are typically referred to as hackathons, workshops or pop-ups and employ rapid design and development practices to tackle technical challenges while engaging with social issues. Based on a collaboration between three digital practitioners (a producer, a researcher and a designer), qualitative and creative data was gathered across five different kinds of TTG events in London and in Tartu which were held in partnership with large institutions, including Art:Work at Tate Exchange within Tate Modern, the Mozilla Festival at Ravensbourne College and the 2017 Association of Internet Researchers conference hosted in Tartu. By analysing data using an open and discursive approach manifested in both text and visual formats, we reflect on the dynamic and generative characteristics of TTG gatherings while also arriving at our own conclusions as situated researchers and practitioners who are ourselves engaged in increasingly messy webs where new worlds of theory and practice are built

Framing the Real: Lefèbvre and NeoRealist Cinematic Space as Practice

In 1945 Roberto Rossellini's Neo-realist Rome, Open City set in motion an approach to cinema and its representation of real life – and by extension real spaces – that was to have international significance in film theory and practice. However, the re-use of the real spaces of the city, and elsewhere, as film sets in Neo-realist film offered (and offers) more than an influential aesthetic and set of cinematic theories. Through Neo-realism, it can be argued that we gain access to a cinematic relational and multidimensional space that is not made from built sets, but by filming the built environment. On the one hand, this space allows us to "notice" the contradictions around us in our cities and, by extension, the societies that have produced those cities, while on the other, allows us to see the spatial practices operative in the production and maintenance of those contradictions. In setting out a template for understanding the spatial practices of Neo-realism through the work of Henri Lefèbvre, this paper opens its films, and those produced today in its wake, to a spatio-political reading of contemporary relevance. We will suggest that the rupturing of divisions between real spaces and the spaces of film locations, as well the blurring of the difference between real life and performed actions for the camera that underlies much of the central importance of Neo-realism, echoes the arguments of Lefèbvre with regard the social production of space. In doing so, we will suggest that film potentially had, and still has, a vital role to play in a critique of contemporary capitalist spatial practices

Developing a Citizen Social Science approach to understand urban stress and promote wellbeing in urban communities

This paper sets out the future potential and challenges for developing an interdisciplinary, mixed-method Citizen Social Science approach to researching urban emotions. It focuses on urban stress, which is increasingly noted as a global mental health challenge facing both urbanised and rapidly urbanising societies. The paper reviews the existing use of mobile psychophysiological or biosensing within urban environments—as means of ‘capturing’ the urban geographies of emotions. Methodological reflections are included on primary research using biosensing in a study of workplace and commuter stress for university employees in Birmingham (UK) and Salzburg (Austria) for illustrative purposes. In comparing perspectives on the conceptualisation and measurement of urban stress from psychology, neuroscience and urban planning, the difficulties of defining scientific constructs within Citizen Science are discussed to set out the groundwork for fostering interdisciplinary dialogue. The novel methods, geo-located sensor technologies and data-driven approaches to researching urban stress now available to researchers pose a number of ethical, political and conceptual challenges around defining and measuring emotions, stress, human behaviour and urban space. They also raise issues of rigour, participation and social scientific interpretation. Introducing methods informed by more critical Citizen Social Science perspectives can temper overly individualised forms of data collection to establish more effective ways of addressing urban stress and promoting wellbeing in urban communities

Nucleic acid-based fluorescent probes and their analytical potential

It is well known that nucleic acids play an essential role in living organisms because they store and transmit genetic information and use that information to direct the synthesis of proteins. However, less is known about the ability of nucleic acids to bind specific ligands and the application of oligonucleotides as molecular probes or biosensors. Oligonucleotide probes are single-stranded nucleic acid fragments that can be tailored to have high specificity and affinity for different targets including nucleic acids, proteins, small molecules, and ions. One can divide oligonucleotide-based probes into two main categories: hybridization probes that are based on the formation of complementary base-pairs, and aptamer probes that exploit selective recognition of nonnucleic acid analytes and may be compared with immunosensors. Design and construction of hybridization and aptamer probes are similar. Typically, oligonucleotide (DNA, RNA) with predefined base sequence and length is modified by covalent attachment of reporter groups (one or more fluorophores in fluorescence-based probes). The fluorescent labels act as transducers that transform biorecognition (hybridization, ligand binding) into a fluorescence signal. Fluorescent labels have several advantages, for example high sensitivity and multiple transduction approaches (fluorescence quenching or enhancement, fluorescence anisotropy, fluorescence lifetime, fluorescence resonance energy transfer (FRET), and excimer-monomer light switching). These multiple signaling options combined with the design flexibility of the recognition element (DNA, RNA, PNA, LNA) and various labeling strategies contribute to development of numerous selective and sensitive bioassays. This review covers fundamentals of the design and engineering of oligonucleotide probes, describes typical construction approaches, and discusses examples of probes used both in hybridization studies and in aptamer-based assays