The E-ELT Multi-Object Spectrograph: latest news from MOSAIC

There are 8000 galaxies, including 1600 at z larger than 1.6, which could be simultaneously observed in an E-ELT field of view of 40 sq. arcmin. A considerable fraction of astrophysical discoveries require large statistical samples, which can only be obtained with multi-object spectrographs (MOS). MOSAIC will provide a vast discovery space, enabled by a multiplex of 200 and spectral resolving powers of R=5000 and 20000. MOSAIC will also offer the unique capability of more than 10 "high-definition" (multi-object adaptive optics, MOAO) integral-field units, optimised to investigate the physics of the sources of reionization. The combination of these modes will make MOSAIC the world-leading MOS facility, contributing to all fields of contemporary astronomy, from extra-solar planets, to the study of the halo of the Milky Way and its satellites, and from resolved stellar populations in nearby galaxies out to observations of the earliest "first-light" structures in the Universe. It will also study the distribution of the dark and ordinary matter at all scales and epochs of the Universe. Recent studies of critical technical issues such as sky-background subtraction and MOAO have demonstrated that such a MOS is feasible with state-of-the-art technology and techniques. Current studies of the MOSAIC team include further trade-offs on the wavelength coverage, a solution for compensating for the non-telecentric new design of the telescope, and tests of the saturation of skylines especially in the near-IR bands. In the 2020s the E-ELT will become the world's largest optical/IR telescope, and we argue that it has to be equipped as soon as possible with a MOS to provide the most efficient, and likely the best way to follow-up on James Webb Space Telescope (JWST) observations.Comment: 10 pages, 3 Figures, in Ground-based and Airborne Instrumentation for Astronomy VI, 2016, Proc. SPI

Differential ontogeny of multiple opioid receptors (μ, δ, and κ)

We investigated the postnatal ontogeny of opioid receptors in rat brain under assay conditions which, when combined with computerized analysis, effectively reflect the developmental profile of high affinity binding to μ, δ, and κ subpopulations. Concentrations of μ sites were assessed with the selective ligand 3H-[D-ala2,mePhe4,gly-ol5]enkephalin (DAGO). The other two sites were analyzed in binding assays with less selective radioligands but in the presence of specific unlabeled ligands which suppress cross-reactivity. We utilized 3H-[D-ala2,D-leu5]enkephalin (DADL) in the presence of 10 nm DAGO to label δ sites and 3H-ethylketocyclazocine (EKC) in the presence of 100 nm DADL + 100 nm [D-ala2,mePhe4,Met(0)ol5]enkepahlin to detect κ receptors. After birth, the density (femtomoles per milligram of wet weight) of μ sites declined for several days and then rose sharply over the next 2 weeks, increasing 2-fold by adulthood. Delta (δ) sites appeared in the second week postnatal and increased more than 8-fold in the next 2 weeks. Levels of κ receptors were relatively low at birth and increased slowly (2-fold, overall). Computerized analyses of binding data revealed that DAGO and DADL were binding to single populations of sites throughout the postnatal period. DAGO and EKC affinities did not fluctuate in this period, whereas DADL affinities were low for the first week and then rose to adult levels. In summary, μ, κ, and δ receptors exhibit differential postnatal developmental profiles. The former two are present at birth, whereas the latter appears in the second week. The postnatal increase for all three sites appear to be preceded by the previously demonstrated emergence of opioid peptides

Evidence for distinct subcellular sites of opiate receptors. Demonstration of opiate receptors in smooth microsomal fractions isolated from rat brain

We have found opiate receptors enriched in two distinct subcellular fractions obtained from rat brain cell-free homogenates. By several criteria, one fraction contains synaptic plasma membranes, while the other represents predominantly smooth microsomes. Using marker enzyme analysis, we determined that the occurrence of opiate receptors in the smooth microsomal fraction cannot be attributed to synaptic plasma membrane cross-contamination. Electron microscopic examination revealed that the two fractions enriched in opiate receptors differed markedly in morphology. In particular, smooth microsomes appeared to lack synaptic junctional complexes, postsynaptic densities, or membranes identifiable as presynaptic. Also, opiate receptors in microsomes were localized on membranes which were distinctly higher (p=1.08-1.14 g/ml) than synaptic plasma membrane receptors (p=1.13-1.18 g/ml) when either agonist or antagonist binding was measured. We also studied the characteristics of binding of opiate agonists to both receptor populations. Although both microsomal and synaptic membrane opiate receptors were sensitive to NaCl when agonist binding was assessed, microsomal receptors were considerably less sensitive toguanine nucleotides than were synaptic membrane receptors. The subsensitivity appeared due to the inability of relatively low concentrations of guanine nucleotides (10 μM) to accelerate the dissociation rate of bound peptide ligand. Finally, microsomal opiate receptors displayed faster rates of dissociation (K-1 = 0.077 min1-) than did synaptic membrane receptors (K-1 = 0.017 min-1). The data are discussed in relation to the concepts that microsomal opiate receptors either represent a distinct population of synaptic membranes or newly synthesized receptors in transit to synaptic membrane sites

Stereospecific opiate-binding sites occur in coated vesicles

We prepared clathrin-coated vesicles from bovine forebrain utilizing sucrose or deuterium oxide-Ficoll density gradient centrifugation followed by permeation chromatography. Homogeneity was monitored by electron microscopy (EM) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). EM revealed that the predominant (up to 98% of the total) organelles were coated vesicles and empty hexagonal baskets. Diameters of the coated vesicles ranged from 37 to 120 nm with a mean of 65.2 ± 2.2. Upon SDS-PAGE of the coated vesicle fraction, the most prominent band appeared at 180,000 daltons. There were also three additional bands at 10,000, 50,000 and 35,000 daltons, giving the overall pattern characteristic of coated vesicles. Both 0.5 nM tritiated naltrexone and etorphine displayed specific binding to coated vesicles. Naltrexone binding in coated vesicles from gradient fractions was increased 2.5-fold over the original 100,000 x g pellet. An additional 4-fold enrichment in specific binding was observed after permeation chromatography which was concomitant with an increase in the volume density of coated vesicles in electron micrographs. Naltrexone binding was stereospecific and etorphine binding was inhibited by 100 mM NaCl (40%). Both naltrexone and etorphine binding were inhibited by 50 μM guanyl-5'-yl imidodiphosphate (40 to 50%). In summary, purified bovine brain-coated vesicles contained high affinity stereospecific opiate alkaloid-binding sites with characteristic opioid binding properties

Chronic Exercise Modifies Age-Related Telomere Dynamics in a Tissue-Specific Fashion

We evaluated the impact of long-term exercise on telomere dynamics in wild-derived short telomere mice (CAST/Ei) over 1 year. We observed significant telomere shortening in liver and cardiac tissues in sedentary 1-year-old mice compared with young (8 weeks) baseline mice that were attenuated in exercised 1-year-old animals. In contrast, skeletal muscle exhibited significant telomere shortening in exercise mice compared with sedentary and young mice. Telomerase enzyme activity was increased in skeletal muscle of exercise compared with sedentary animals but was similar in cardiac and liver tissues. We observed significant age-related decreases in expression of telomere-related genes that were attenuated by exercise in cardiac and skeletal muscle but not liver. Protein content of TRF1 was significantly increased in plantaris muscle with age. In summary, long-term exercise altered telomere dynamics, slowing age-related decreases in telomere length in cardiac and liver tissue but contributing to shortening in exercised skeletal muscle

A methodology for measuring the sustainability of car transport systems

Measuring the sustainability of car fleets, an important task in developing transport policy, can be accomplished with an appropriate set of indicators. We applied the Process Analysis Method of sustainability assessment to generate an indicator set in a systematic and transparent way, that is consistent with a declared definition of a sustainable transport system. Our method identifies stakeholder groups, the full range of impacts across the environmental, economic and human/social domains of sustainability, and those who generate and receive those impacts. Car users are shown by the analysis to have dual roles, both as individual makers of decisions and as beneficiaries/sufferers of the impacts resulting from communal choice. Thus car users, through their experience of service quality, are a potential force for system change. Our method addresses many of the well-known flaws in measuring transport sustainability. The indicator set created is independent of national characteristics and will be useful to transport policy practitioners and sustainable mobility researchers globally. © 2013 Elsevier Ltd

Detection and characterization of β-adrenergic receptors and adenylate cyclase in coated vesicles isolated from bovine brain

[No abstract available

Pairing in two-dimensional boson-fermion mixtures

The possibilities of pairing in two-dimensional boson-fermion mixtures are carefully analyzed. It is shown that the boson-induced attraction between two identical fermions dominates the p-wave pairing at low density. For a given fermion density, the pairing gap becomes maximal at a certain optimal boson concentration. The conditions for observing pairing in current experiments are discussedComment: 10 pages, 5 figs, revtex

Aquatic food security:insights into challenges and solutions from an analysis of interactions between fisheries, aquaculture, food safety, human health, fish and human welfare, economy and environment

Fisheries and aquaculture production, imports, exports and equitability of distribution determine the supply of aquatic food to people. Aquatic food security is achieved when a food supply is sufficient, safe, sustainable, shockproof and sound: sufficient, to meet needs and preferences of people; safe, to provide nutritional benefit while posing minimal health risks; sustainable, to provide food now and for future generations; shock-proof, to provide resilience to shocks in production systems and supply chains; and sound, to meet legal and ethical standards for welfare of animals, people and environment. Here, we present an integrated assessment of these elements of the aquatic food system in the United Kingdom, a system linked to dynamic global networks of producers, processors and markets. Our assessment addresses sufficiency of supply from aquaculture, fisheries and trade; safety of supply given biological, chemical and radiation hazards; social, economic and environmental sustainability of production systems and supply chains; system resilience to social, economic and environmental shocks; welfare of fish, people and environment; and the authenticity of food. Conventionally, these aspects of the food system are not assessed collectively, so information supporting our assessment is widely dispersed. Our assessment reveals trade-offs and challenges in the food system that are easily overlooked in sectoral analyses of fisheries, aquaculture, health, medicine, human and fish welfare, safety and environment. We highlight potential benefits of an integrated, systematic and ongoing process to assess security of the aquatic food system and to predict impacts of social, economic and environmental change on food supply and demand