Multi-source hierarchical conditional random field model for feature fusion of remote sensing images and LiDAR data

Feature fusion of remote sensing images and LiDAR points cloud data, which have strong complementarity, can effectively play the advantages of multi-class features to provide more reliable information support for the remote sensing applications, such as object classification and recognition. In this paper, we introduce a novel multi-source hierarchical conditional random field (MSHCRF) model to fuse features extracted from remote sensing images and LiDAR data for image classification. Firstly, typical features are selected to obtain the interest regions from multi-source data, then MSHCRF model is constructed to exploit up the features, category compatibility of images and the category consistency of multi-source data based on the regions, and the outputs of the model represents the optimal results of the image classification. Competitive results demonstrate the precision and robustness of the proposed method

Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia

Purpose Congenital diaphragmatic hernia (CDH) is characterized by a developmental defect in the diaphragm, pulmonary hypoplasia and pulmonary hypertension. NPAS3 is a PAS domain transcription factor regulating Drosophila tracheogenesis. NPAS3 null mice develop pulmonary hypoplasia in utero and die after birth due to respiratory failure. We aimed to evaluate NPAS3 expres- sion during normal and abnormal lung development due to CDH. Methods CDH was induced by administering 100 mg/ml nitrofen to time-pregnant dams on embryonic day (E) 9 of gestation. Lungs were isolated on E15, E18 and E21 and NPAS3 localization was determined by immunohisto- chemistry and quantified using Western blotting. Results We found that only E21 hypoplastic CDH lungs have reduced expression of NPAS3 in the terminal sac- cules. Western blotting confirmed the down-regulation of NPAS3 protein in the nitrofen-induced hypoplastic lungs. Conclusions We demonstrate for the first time that ni- trofen-induced hypoplastic CDH lungs have reduced NPAS3 expression in the terminal saccules during the later stages of abnormal lung development. Our findings suggest that NPAS3 is associated with pulmonary hypoplasia in CDH.Supported by the Children’s Hospital Research Institute of Manitoba; RK is the recipient of a Career Enhancement Award from the Canadian Child Health Clinician Scientist Program and a New Investigator Salary Award from the Canadian Institutes of Health Research, Manitoba Lung Association and the Children’s Hospital Research Institute

Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor

We present a light-switchable gene expression system for both inducible and switchable control of gene expression at a single cell level in Escherichia coli using a previously constructed light-sensing system. The lambda cl repressor gene with an LVA degradation tag was expressed under the control of the ompC promoter on the chromosome. The green fluorescent protein (GFP) gene fused to a lambda repressor-repressible promoter was used as a reporter. This light-switchable system allows rapid and reversible induction or repression of expression of the target gene at any desired time. This system also ensures homogenous expression across the entire cell population. We also report the design of a miniaturized photobioreactor to be used in combination with the light-switchable gene expression system. The miniaturized photobioreactor helps to reduce unintended induction of the light receptor due to environmental disturbances and allows precise control over the duration of induction. This system would be a good tool for switchable, homogenous, strong, and highly regulatable expression of target genes over a wide range of induction times. Hence, it could be applied to study gene function, optimize metabolic pathways, and control biological systems both spatially and temporally.open0

Study of e+e−→ppˉe^+e^- \rightarrow p\bar{p} in the vicinity of ψ(3770)\psi(3770)

Using 2917 $\rm{pb}^{-1}$ of data accumulated at 3.773~$\rm{GeV}$, 44.5~$\rm{pb}^{-1}$ of data accumulated at 3.65~$\rm{GeV}$ and data accumulated during a $\psi(3770)$ line-shape scan with the BESIII detector, the reaction $e^+e^-\rightarrow p\bar{p}$ is studied considering a possible interference between resonant and continuum amplitudes. The cross section of $e^+e^-\rightarrow\psi(3770)\rightarrow p\bar{p}$, $\sigma(e^+e^-\rightarrow\psi(3770)\rightarrow p\bar{p})$, is found to have two solutions, determined to be ($0.059\pm0.032\pm0.012$) pb with the phase angle $\phi = (255.8\pm37.9\pm4.8)^\circ$ ($<$0.11 pb at the 90% confidence level), or $\sigma(e^+e^-\rightarrow\psi(3770)\rightarrow p\bar{p}) = (2.57\pm0.12\pm0.12$) pb with $\phi = (266.9\pm6.1\pm0.9)^\circ$ both of which agree with a destructive interference. Using the obtained cross section of $\psi(3770)\rightarrow p\bar{p}$, the cross section of $p\bar{p}\rightarrow \psi(3770)$, which is useful information for the future PANDA experiment, is estimated to be either ($9.8\pm5.7$) nb ($<17.2$ nb at 90% C.L.) or $(425.6\pm42.9)$ nb

Transfer function development for control of cathode airflow transients in fuel cell gas turbine hybrid systems

Direct-fired fuel cell gas turbine hybrid power system responses to open-loop transients were evaluated using a hardware-based simulation of an integrated solid oxide fuel cell gas turbine (SOFC/GT) hybrid system, implemented through the Hybrid Performance (Hyper) facility at the U.S. Department of Energy, National Energy Technology Laboratory (NETL). A disturbance in the cathode inlet air mass flow was performed by manipulating a hot-air bypass valve implemented in the hardware component. Two tests were performed; the fuel cell stack subsystem numerical simulation model was both decoupled and fully coupled with the gas turbine hardware component. The dynamic responses of the entire SOFC/GT hybrid system were studied in this paper. The reduction of cathode airflow resulted in a sharp decrease and partial recovery of the fuel cell thermal effluent in 10 s. In contrast, the turbine rotational speed did not exhibit a similar trend. The transfer functions of several important variables in the fuel cell stack subsystem and gas turbine subsystem were developed to be used in the future control method development. The importance of the cathode airflow regulation was quantified through transfer functions. The management of cathode airflow was also suggested to be a potential strategy to increase the life of fuel cells by reducing the thermal impact of operational transients on the fuel cell subsystem

The CID1 cyclin C-like gene is important for plant infection in Fusarium graminearum

Head blight or scab caused by Fusarium graminearum is a destructive disease of wheat and barley. The pathogen can cause severe yield losses and contaminates infested kernels with harmful mycotoxins. In this study, we characterized the CID1 gene in F. graminearum that is an ortholog of the Fusarium verticilloides FCC1 and yeast UME3 genes. The protein encoded by CID1 has typical structural features of C-type cyclins. Deletion of CID1 resulted in a reduction in conidiation and vegetative growth but an increase in pigmentation. The ¿cid1 mutant was female sterile but could outcross as a male. It was significantly reduced in DON production and virulence on wheat heads and corn stalks. Only about 50% of inoculated spikelets developed scab symptoms and scab disease rarely extended to nearby florets, suggesting that the ¿cid1 mutant was defective in colonizing and spreading in wheat heads. Deletion of CID1 resulted in reduced expression levels of TRI5 and TRI101 but increased PKS12 expression. When expressed in F. verticillioides, the CID1 gene complemented the defects of the ¿fcc1 mutant in conidiation, hyphal growth, and fumonisin production. Our data indicate that the CID1 C-type cyclin gene plays multiple roles in the regulation of vegetative growth, sexual development, conidiation, DON production, and pathogenicity

Drivers of the low-cloud response to poleward jet shifts in the North Pacific in observations and models

The long-standing expectation that poleward shifts of the midlatitude jet under global warming will lead to poleward shifts of clouds and a positive radiative feedback on the climate system has been shown to be misguided by several recent studies. On interannual time scales, free-tropospheric clouds are observed to shift along with the jet, but low clouds increase across a broad expanse of the North Pacific Ocean basin, resulting in negligible changes in total cloud fraction and top-of-atmosphere radiation. Here it is shown that this low-cloud response is consistent across eight independent satellite-derived cloud products. Using multiple linear regression, it is demonstrated that the spatial pattern and magnitude of the low-cloud-coverage response is primarily driven by anomalous surface temperature advection. In the eastern North Pacific, anomalous cold advection by anomalous northerly surface winds enhances sensible and latent heat fluxes from the ocean into the boundary layer, resulting in large increases in low-cloud coverage. Local increases in low-level stability make a smaller contribution to this low-cloud increase. Despite closely capturing the observed response of large-scale meteorology to jet shifts, global climate models largely fail to capture the observed response of clouds and radiation to interannual jet shifts because they systematically underestimate how sensitive low clouds are to surface temperature advection, and to a lesser extent, low-level stability. More realistic model simulations of cloud–radiation–jet interactions require that parameterizations more accurately capture the sensitivity of low clouds to surface temperature advection

Understanding the structure of the first atomic contact in gold

We have studied experimentally jump-to-contact (JC) and jump-out-of-contact (JOC) phenomena in gold electrodes. JC can be observed at first contact when two metals approach each other, while JOC occurs in the last contact before breaking. When the indentation depth between the electrodes is limited to a certain value of conductance, a highly reproducible behaviour in the evolution of the conductance can be obtained for hundreds of cycles of formation and rupture. Molecular dynamics simulations of this process show how the two metallic electrodes are shaped into tips of a well-defined crystallographic structure formed through a mechanical annealing mechanism. We report a detailed analysis of the atomic configurations obtained before contact and rupture of these stable structures and obtained their conductance using first-principles quantum transport calculations. These results help us understand the values of conductance obtained experimentally in the JC and JOC phenomena and improve our understanding of atomic-sized contacts and the evolution of their structural characteristics.This work was supported by the Spanish government through grants FIS2010-21883, CONSOLIDER CSD2007-0010, Generalitat Valenciana through PROMETEO/2012/011, ACOMP/2012/127 and Feder funds from E.U