Axillary bud viability and dry matter production of Poa ligularis in Patagonian grasslands

Poa ligularis Nees is one of the dominant perennial grass species in Patagonian grasslands. This is the first field study which investigated the effects of defoliation frequency on its number of viable axillary buds, and subsequent dry matter production during one growing season. Experiments were arranged in a randomized complete block design. Treatments included one defoliation height (10 cm), from one to five times a year, and undefoliated controls. Bud respiratory activity was examined usingthe tetrazolium test and the vital stain Evan's blue. At the end of the growing season, plants that have been defoliated more than three times showed significatively (p<0.05) lower number of respiratory active buds than unclipped controls. A high defoliation frequency increased bud death in stem bases. Both defoliated and undefoliated plants produced more (p<0.05) axillary buds at the periphery than at their center. Defoliation treatments increased shoot weight by 13.4% on average compared with undefoliated controls, but reduced in by 10% the average number of metabolically active axillary buds. This indicates that this reduction, however, was not high enough to constrain dry matter production.It appears that Poa ligularis could tolerate moderate grazing frequency and intensity without compromising its potential regrowth capacity. Longer-term studies of the effects of defoliation on dry matter production and bud metabolic activity are needed to understand the cumulative effects of grazing of P. ligularis in natural grasslands.Poa ligularis Nees es una de las especies de gramíneas perennes dominantes en los pastizales naturales de la Patagonia. Este es el primer estudio que investigó los efectos de la frequencia de defoliación sobre su número de yemas axilares viables, y subsiguiente producción de materia seca durante una estación de crecimiento. Los estudios se efectuaron utilizando un diseño de bloques completamente al azar. Los tratamientos incluyeron una altura de defoliación (10 cm), de una a cinco veces por año, además de controles no defoliados. La actividad respiratoria de las yemas fue examinada usando el método del tetrazolio y el colorante vital azul de Evans.Hacia el final de la estación de crecimiento, las plantas que habían sido defoliadas más de tres veces mostraron un número significativamente menor (p<0.05) de yemas con actividad respiratoria que los controles no defoliados. Una alta frecuencia de defoliación incrementó la muerte de yemas en las bases de tallos. Todas las plantas- defoliadas o no, produjeron más (p<0.05) yemas axilares en su periferia que en su centro. La defoliación incrementó 13.4% el peso de los tallos en promedio, pero redujo en 10% el número promedio de yemas axilares metabólicamente activas. Esto indica que esta reducción , sin embargo, no fue suficiente como para limitar la producción de materia seca. Parece que P. ligularis toleraría una frecuencia e intensidad moderada de pastoreo sin comprometer su capacidad potencial de rebrote. Se necesitan estudios a más largo plazo de los efectos de la defoliación en la producción de materia seca y actividad metabólica de las yemas para entender los efectos acumulativos del pastoreo de Poa ligularis en los pastizales naturales.Fil: Souto, Cintia Paola. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Becker, G. F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Siffredi, G. L.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Sterberg, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentin

Evolution, Explosion and Nucleosynthesis of Core Collapse Supernovae

We present a new set of presupernova evolutions and explosive yields of massive stars of initial solar composition (Y=0.285, Z=0.02) in the mass range 13-35 Msun. All the models have been computed with the latest version (4.97) of the FRANEC code that now includes a nuclear network extending from neutrons to Mo98. The explosive nucleosynthesis has been computed twice: a first one with an hydro code and a second one following the simpler radiation dominated shock approximation (RDA).Comment: 20 pages, 10 figures, 12 tables. Accepted for publication on Ap

The effects of a revised 7^7Be e−^--capture rate on solar neutrino fluxes

The electron-capture rate on $^7$Be is the main production channel for $^7$Li in several astrophysical environments. Theoretical evaluations have to account for not only the nuclear interaction, but also the processes in the plasma where $^7$Be ions and electrons interact. In the past decades several estimates were presented, pointing out that the theoretical uncertainty in the rate is in general of few percents. In the framework of fundamental solar physics, we consider here a recent evaluation for the $^7$Be+e$^-$ rate, not used up to now in the estimate of neutrino fluxes. We analysed the effects of the new assumptions on Standard Solar Models (SSMs) and compared the results obtained by adopting the revised $^7$Be+e$^-$ rate to those obtained by the one reported in a widely used compilation of reaction rates (ADE11). We found that new SSMs yield a maximum difference in the efficiency of the $^7$Be channel of about -4\% with respect to what is obtained with the previously adopted rate. This fact affects the production of neutrinos from $^8$B, increasing the relative flux up to a maximum of 2.7\%. Negligible variations are found for the physical and chemical properties of the computed solar models. The agreement with the SNO measurements of the neutral current component of the $^8$B neutrino flux is improved.Comment: 7 pages, 3 figures, 4 tables. Accepted for the publication on A&

Efecto del estrés producido por la mezcla de sales en la concentración de aldehído malónico, proteínas y enzimas antioxidantes de Leymus chinensis de tres colores foliares diferentes

The mixed salt stress is common in nature. Salt stressalways affects plant growth. Different plant species have different adaptive capacity to salty soil. Leymus chinensis is an herbaceous plant with different leaf colors. However, little research was conducted to explore the different tolerance mechanisms to salt stress among the three different leaf colour genotypes of Leymus chinensis (grey green, transitional color, yellow green). Pot experiments for Leymus chinensis in three leaf colors were conducted under mixed salt treatments in 2010. Malondialdehyde (MDA) and protein concentrations, and the activity of various antioxidant enzymes [i.e., superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDHAR)] were determined and compared among the three leaf color genotypes of Leymus chinensis. The concentrations of MDA and protein, and the activity of antioxidant enzymes showed an increasing trend with increasing pHs in almost all three leaf colors, and all of them became highest when salt stress and pH values were also highest. Moreover, antioxidant enzymes were the highest in the grey-green leaf color, and the lowest in the yellow green leaf color after exposure to the same pH treatment. The results suggested that all three leaf colors of Leymus chinensis were tolerant to salt stress, and the salt-tolerance declined according to the order of grey green > transitional color > yellow green of Leymus chinensis. This study can give us a better understanding of the intra-species adaptation to mixed salt soils.El estrés causado por mezcla de sales en el suelo es común en la naturaleza. El estrés salino siempre afecta el crecimiento de las plantas. Plantas de especies diferentes difieren en su capacidad de adaptación al estrés por sales en el suelo. Leymus chinensis es una planta herbácea con diferentes colores foliares. Sin embargo, se han conducido pocos estudios tendientes a determinar los diferentes mecanismos de tolerancia al estrés salino entre los tres genotipos de color foliar diferente de L. chinensis (grisáceo verdoso, color intermedio, amarillo verdoso). En 2010, se condujeron experimentos en macetas usando genotipos de L. chinensis de tres colores diferentes de hoja expuestos o no a tratamientos conteniendo una mezcla de sales. Las concentraciones de aldehído malónico (MDA) y proteínas, y la actividad de varias enzimas antioxidantes [es decir, la superóxido dismutasa (SOD), catalasa (CAT), ascórbico peroxidasa (APX), glutatión reductasa (GR), dehidroascórbico reductasa (DHAR) y monodehidroascórbico reductasa (MDHAR)] se determinaron y compararon entre los tres genotipos de color foliar diferente de L. chinensis. Las concentraciones de MDA y proteínas, y la actividad de enzimas antioxidantes mostraron una tendencia a incrementarse a mayores pHs en casi todos los colores foliares, y las tendencias en los tres colores foliares alcanzaron su punto máximo cuando el estrés salino y los valores de pH fueron máximos. Más aún, las concentraciones de las enzimas antioxidantes fueron las más altas en el color grisáceo verdoso, intermedias en el color intermedio, y las más bajas en el color amarillo verdoso después de la exposición al mismo tratamiento de pH. Los resultados sugirieron que los genotipos de los tres colores foliares de L. chinensis fueros tolerantes al estrés salino, y la tolerancia a la sal declinó de acuerdo al orden grisáceo verdoso > color intermedio > amarillo verdoso de L. chinensis. Este estudio puede proveer un mejor entendimiento de la adaptación intraespecífica de L. chinensis a suelos salinos.Fil: Zhou, C.. Chinese Academy of Science; China. Liaoning University; ChinaFil: Busso, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Yang, Y. G.. Liaoning University; ChinaFil: Zhang, Z.. Shenyang University; ChinaFil: Wang, Z. W.. Chinese Academy of Science; ChinaFil: Yang, Y. F.. Northeast Normal University; ChinaFil: Han, X. G.. Chinese Academy of Science; Chin

Sodium Thiosulfate Prevents Chondrocyte Mineralization and Reduces the Severity of Murine Osteoarthritis.

Calcium-containing crystals participate in the pathogenesis of OA. Sodium thiosulfate (STS) has been shown to be an effective treatment in calcification disorders such as calciphylaxis and vascular calcification. This study investigated the effects and mechanisms of action of STS in a murine model of OA and in chondrocyte calcification. Hydroxyapatite (HA) crystals-stimulated murine chondrocytes and macrophages were treated with STS. Mineralization and cellular production of IL-6, MCP-1 and reactive oxygen species (ROS) were assayed. STS's effects on genes involved in calcification, inflammation and cartilage matrix degradation were studied by RT-PCR. STS was administered in the menisectomy model of murine OA, and the effect on periarticular calcific deposits and cartilage degeneration was investigated by micro-CT-scan and histology. In vitro, STS prevented in a dose-dependent manner calcium crystal deposition in chondrocytes and inhibited Annexin V gene expression. In addition, there was a reduction in crystal-induced IL-6 and MCP-1 production. STS also had an antioxidant effect, diminished HA-induced ROS generation and abrogated HA-induced catabolic responses in chondrocytes. In vivo, administration of STS reduced the histological severity of OA, by limiting the size of new periarticular calcific deposits and reducing the severity of cartilage damage. STS reduces the severity of periarticular calcification and cartilage damage in an animal model of OA via its effects on chondrocyte mineralization and its attenuation of crystal-induced inflammation as well as catabolic enzymes and ROS generation. Our study suggests that STS may be a disease-modifying drug in crystal-associated OA

Abundance gradients in the Milky Way for alpha elements, Iron peak elements, Barium, Lanthanum and Europium

We model the abundance gradients in the disk of the Milky Way for several chemical elements (O, Mg, Si, S, Ca, Sc, Ti, Co, V, Fe, Ni, Zn, Cu, Mn, Cr, Ba, La and Eu), and compare our results with the most recent and homogeneous observational data. We adopt a chemical evolution model able to well reproduce the main properties of the solar vicinity. We compute, for the first time, the abundance gradients for all the above mentioned elements in the galactocentric distance range 4 - 22 kpc. The comparison with the observed data on Cepheids in the galactocentric distance range 5-17 kpc gives a very good agreement for many of the studied elements. In addition, we fit very well the data for the evolution of Lanthanum in the solar vicinity for which we present results here for the first time. We explore, also for the first time, the behaviour of the abundance gradients at large galactocentric distances by comparing our results with data relative to distant open clusters and red giants and select the best chemical evolution model model on the basis of that. We find a very good fit to the observed abundance gradients, as traced by Cepheids, for most of the elements, thus confirming the validity of the inside-out scenario for the formation of the Milky Way disk as well as the adopted nucleosynthesis prescriptions.Comment: 11 pages, 9 figures, accepted for publication in A&

The early evolution of Globular Clusters: the case of NGC 2808

Enhancement and spread of helium among globular cluster stars have been recently suggested as a way to explain the horizontal branch blue tails, in those clusters which show a primordial spread in the abundances of CNO and other elements involved in advanced CNO burning (D'Antona et al. 2002). In this paper we examine the implications of the hypothesis that, in many globular clusters, stars were born in two separate events: an initial burst (first generation), which gives origin to probably all high and intermediate mass stars and to a fraction of the cluster stars observed today, and a second, prolonged star formation phase (second generation) in which stars form directly from the ejecta of the intermediate mass stars of the first generation. In particular, we consider in detail the morphology of the horizontal branch in NGC 2808 and argue that it unveils the early cluster evolution, from the birth of the first star generation to the end of the second phase of star formation. This framework provides a feasible interpretation for the still unexplained dichotomy of NGC 2808 horizontal branch, attributing the lack of stars in the RR Lyr region to the gap in the helium content between the red clump, whose stars are considered to belong to the first stellar generation and have primordial helium, and the blue side of the horizontal branch, whose minimum helium content reflects the helium abundance in the smallest mass (~4Msun)contributing to the second stellar generation. This scenario provides constraints on the required Initial Mass Function, in a way that a great deal of remnant neutron stars and stellar mass black holes might have been produced.Comment: 23 pages, 7 figures, in press on The Astrophysical Journa