Individual tracking reveals long-distance flight-path control in a nocturnally migrating moth

Each year, trillions of insects make long-range seasonal migrations. These movements are relatively well understood at a population level, but how individual insects achieve them remains elusive. Behavioral responses to conditions en route are little studied, primarily owing to the challenges of tracking individual insects. Using a light aircraft and individual radio tracking, we show that nocturnally migrating death's-head hawkmoths maintain control of their flight trajectories over long distances. The moths did not just fly with favorable tailwinds; during a given night, they also adjusted for head and crosswinds to precisely hold course. This behavior indicates that the moths use a sophisticated internal compass to maintain seasonally beneficial migratory trajectories independent of wind conditions, illuminating how insects traverse long distances to take advantage of seasonal resources

The night-sky at the Calar Alto Observatory

We present a characterization of the main properties of the night-sky at the Calar Alto observatory for the time period between 2004 and 2007. We use optical spectrophotometric data, photometric calibrated images taken in moonless observing periods, together with the observing conditions regularly monitored at the observatory, such as atmospheric extinction and seeing. We derive, for the first time, the typical moonless night-sky optical spectrum for the observatory. The spectrum shows a strong contamination by different pollution lines, in particular from Mercury lines, which contribution to the sky-brightness in the different bands is of the order of ~0.09 mag, ~0.16 mag and ~0.10 mag in B, V and R respectively. The zenith-corrected values of the moonless night-sky surface brightness are 22.39, 22.86, 22.01, 21.36 and 19.25 mag arcsec^-2 in U, B, V, R and I, which indicates that Calar Alto is a particularly dark site for optical observations up to the I-band. The fraction of astronomical useful nights at the observatory is ~70%, with a ~30% of photometric nights. The typical extinction at the observatory is k_V~0.15 mag in the Winter season, with little dispersion. In summer the extinction has a wider range of values, although it does not reach the extreme peaks observed at other sites. The median seeing for the last two years (2005-6) was ~0.90", being smaller in the Summer (~0.87") than in the Winter (~0.96"). We conclude in general that after 26 years of operations Calar Alto is still a good astronomical site, being a natural candidate for future large aperture optical telescopes.Comment: 16 pages, 5 figures, accepted for publishing in the Publications of Astronomical Society of the Pacific (PASP

Velocity Dispersion of Dissolving OB Associations Affected by External Pressure of Formation Environment

This paper presents a possible way to understand dissolution of OB associations (or groups). Assuming rapid escape of parental cloud gas from associations, we show that the shadow of the formation environment for associations can be partially imprinted on the velocity dispersion at their dissolution. This conclusion is not surprising as long as associations are formed in a multiphase interstellar medium, because the external pressure should suppress expansion caused by the internal motion of the parental clouds. Our model predicts a few km s$^{-1}$ as the internal velocity dispersion. Observationally, the internal velocity dispersion is $\sim 1$ km s$^{-1}$ which is smaller than our prediction. This suggests that the dissipation of internal energy happens before the formation of OB associations.Comment: 6 pages. AJ accepte

In Search of Possible Associations between Planetary Nebulae and Open Clusters

We consider the possibility of cluster membership for 13 planetary nebulae that are located in close proximity to open clusters lying in their lines of sight. The short lifetimes and low sample size of intermediate-mass planetary nebulae with respect to nearby open clusters conspire to reduce the probability of observing a true association. Not surprisingly, line of sight coincidences almost certainly exist for 7 of the 13 cases considered. Additional studies are advocated, however, for 6 planetary nebula/open cluster coincidences in which a physical association is not excluded by the available evidence, namely M 1-80/Berkeley 57, NGC 2438/NGC 2437, NGC 2452/NGC 2453, VBRC 2 & NGC 2899/IC 2488, and HeFa 1/NGC 6067. A number of additional potential associations between planetary nebulae and open clusters is tabulated for reference purposes. It is noteworthy that the strongest cases involve planetary nebulae lying in cluster coronae, a feature also found for short-period cluster Cepheids, which are themselves potential progenitors of planetary nebulae.Comment: Accepted for publication in PASP (December 2007

Mitochondrial targeting adaptation of the hominoid-specific glutamate dehydrogenase driven by positive Darwinian selection

Many new gene copies emerged by gene duplication in hominoids, but little is known with respect to their functional evolution. Glutamate dehydrogenase (GLUD) is an enzyme central to the glutamate and energy metabolism of the cell. In addition to the single, GLUD-encoding gene present in all mammals (GLUD1), humans and apes acquired a second GLUD gene (GLUD2) through retroduplication of GLUD1, which codes for an enzyme with unique, potentially brain-adapted properties. Here we show that whereas the GLUD1 parental protein localizes to mitochondria and the cytoplasm, GLUD2 is specifically targeted to mitochondria. Using evolutionary analysis and resurrected ancestral protein variants, we demonstrate that the enhanced mitochondrial targeting specificity of GLUD2 is due to a single positively selected glutamic acid-to-lysine substitution, which was fixed in the N-terminal mitochondrial targeting sequence (MTS) of GLUD2 soon after the duplication event in the hominoid ancestor ~18–25 million years ago. This MTS substitution arose in parallel with two crucial adaptive amino acid changes in the enzyme and likely contributed to the functional adaptation of GLUD2 to the glutamate metabolism of the hominoid brain and other tissues. We suggest that rapid, selectively driven subcellular adaptation, as exemplified by GLUD2, represents a common route underlying the emergence of new gene functions

Polaris the Cepheid returns: 4.5 years of monitoring from ground and space

We present the analysis of 4.5 years of nearly continuous observations of the classical Cepheid Polaris, which comprise the most precise data available for this star. We have made spectroscopic measurements from ground and photometric measurements from the WIRE star tracker and the SMEI instrument on the Coriolis satellite. Measurements of the amplitude of the dominant oscillation (P = 4 days), that go back more than a century, show a decrease from 120 mmag to 30 mmag (V magnitude) around the turn of the millennium. It has been speculated that the reason for the decrease in amplitude is the evolution of Polaris towards the edge of the instability strip. However, our new data reveal an increase in the amplitude by about 30% from 2003-2006. It now appears that the amplitude change is cyclic rather than monotonic, and most likely the result of a pulsation phenomenon. In addition, previous radial velocity campaigns have claimed the detection of long-period variation in Polaris (P > 40 days). Our radial velocity data are more precise than previous datasets, and we find no evidence for additional variation for periods in the range 3-50 days with an upper limit of 100 m/s. However, in the WIRE data we find evidence of variation on time-scales of 2-6 days, which we interpret as being due to granulation.Comment: Accepted by ApJ. 8 pages. Quality of figures 1+3+7 degrade

Phylogenomics Reshuffles the Eukaryotic Supergroups

Background. Resolving the phylogenetic relationships between eukaryotes is an ongoing challenge of evolutionary biology. In recent years, the accumulation of molecular data led to a new evolutionary understanding, in which all eukaryotic diversity has been classified into five or six supergroups. Yet, the composition of these large assemblages and their relationships remain controversial. Methodology/Principle Findings. Here, we report the sequencing of expressed sequence tags (ESTs) for two species belonging to the supergroup Rhizaria and present the analysis of a unique dataset combining 29908 amino acid positions and an extensive taxa sampling made of 49 mainly unicellular species representative of all supergroups. Our results show a very robust relationship between Rhizaria and two main clades of the supergroup chromalveolates: stramenopiles and alveolates. We confirm the existence of consistent affinities between assemblages that were thought to belong to different supergroups of eukaryotes, thus not sharing a close evolutionary history. Conclusions. This well supported phylogeny has important consequences for our understanding of the evolutionary history of eukaryotes. In particular, it questions a single red algal origin of the chlorophyll-c containing plastids among the chromalveolates. We propose the abbreviated name ‘SAR’ (Stramenopiles+Alveolates+Rhizaria) to accommodate this new super assemblage of eukaryotes, which comprises the largest diversity of unicellular eukaryotes

Functional significance may underlie the taxonomic utility of single amino acid substitutions in conserved proteins

We hypothesized that some amino acid substitutions in conserved proteins that are strongly fixed by critical functional roles would show lineage-specific distributions. As an example of an archetypal conserved eukaryotic protein we considered the active site of ß-tubulin. Our analysis identified one amino acid substitution—ß-tubulin F224—which was highly lineage specific. Investigation of ß-tubulin for other phylogenetically restricted amino acids identified several with apparent specificity for well-defined phylogenetic groups. Intriguingly, none showed specificity for “supergroups” other than the unikonts. To understand why, we analysed the ß-tubulin Neighbor-Net and demonstrated a fundamental division between core ß-tubulins (plant-like) and divergent ß-tubulins (animal and fungal). F224 was almost completely restricted to the core ß-tubulins, while divergent ß-tubulins possessed Y224. Thus, our specific example offers insight into the restrictions associated with the co-evolution of ß-tubulin during the radiation of eukaryotes, underlining a fundamental dichotomy between F-type, core ß-tubulins and Y-type, divergent ß-tubulins. More broadly our study provides proof of principle for the taxonomic utility of critical amino acids in the active sites of conserved proteins

The MACHO Project LMC Variable Star Inventory. VI. The Second-overtone Mode of Cepheid Pulsation From First/Second Overtone (FO/SO) Beat Cepheids

MACHO Project photometry of 45 LMC FO/SO beat Cepheids which pulsate in the first and second overtone (FO and SOo, respectively) has been analysed to determine the lightcurve characteristics for the SO mode of Cepheid pulsation. We predict that singly-periodic SO Cepheids will have nearly sinusoidal lightcurves; that we will only be able to discern SO Cepheids from fundamental (F) and (FO) Cepheids for P <= 1.4 days; and that the SO distribution will overlap the short-period edge of the LMC FO Cepheid period-luminosity relation (when both are plotted as a function of photometric period). We also report the discovery of one SO Cepheid candidate, MACHO*05:03:39.6$-$70:04:32, with a photometric period of 0.775961 +/- 0.000019 days and an instrumental amplitude of 0.047 +/- 0.009 mag in V.Comment: 23 pages, 7 Encapsulated PostScript figures. Accepted for publication in the Astrophysical Journa

Cepheid limb darkening, angular diameter corrections, and projection factor from static spherical model stellar atmospheres

Context. One challenge for measuring the Hubble constant using Classical Cepheids is the calibration of the Leavitt Law or period-luminosity relationship. The Baade-Wesselink method for distance determination to Cepheids relies on the ratio of the measured radial velocity and pulsation velocity, the so-called projection factor and the ability to measure the stellar angular diameters. Aims. We use spherically-symmetric model stellar atmospheres to explore the dependence of the p-factor and angular diameter corrections as a function of pulsation period. Methods. Intensity profiles are computed from a grid of plane-parallel and spherically-symmetric model stellar atmospheres using the SAtlas code. Projection factors and angular diameter corrections are determined from these intensity profiles and compared to previous results. Results. Our predicted geometric period-projection factor relation including previously published state-of-the-art hydrodynamical predictions is not with recent observational constraints. We suggest a number of potential resolutions to this discrepancy. The model atmosphere geometry also affects predictions for angular diameter corrections used to interpret interferometric observations, suggesting corrections used in the past underestimated Cepheid angular diameters by 3 - 5%. Conclusions. While spherically-symmetric hydrostatic model atmospheres cannot resolve differences between projection factors from theory and observations, they do help constrain underlying physics that must be included, including chromospheres and mass loss. The models also predict more physically-based limb-darkening corrections for interferometric observations.Comment: 8 pages, 6 figures, 2 tables, accepted for publication in A&