Parasitoid complex of fall armyworm, spodoptera frugiperda, in Ghana and Benin

Open Access Journal; Published online: 21 Jan 2020The fall armyworm, Spodoptera frugiperda, a moth originating from the American continent, has recently invaded most African countries, where it is seriously threatening food security as a pest of cereals. The current management methods rely heavily on the use of synthetic insecticides but there is a need for more sustainable control methods, including biological control. Surveys were conducted in two West African countries, Ghana and Benin, to determine the native parasitoid complex and assess parasitism rates of S. frugiperda. Samples of S. frugiperda eggs and larvae were collected in maize fields located in 56 and 90 localities of Ghana and Benin, respectively, from July 2018 to July 2019. Ten species were found parasitizing the pest, including two egg parasitoids, one egg–larval, five larval and two larval–pupal parasitoids. The two most abundant parasitoids in both countries were two Braconidae: the egg‐larval parasitoid Chelonus bifoveolatus and the larval parasitoid Coccygidum luteum. Parasitism rates were determined in three Ghanaian regions and averages varied from 0% to 75% between sites and from 5% to 38% between regions. These data provide an important baseline for the development of various biological control options. The two egg parasitoids, Telenomus remus and Trichogramma sp. can be used in augmentative biological control and investigations should be conducted to assess how cultural practices can enhance the action of the main parasitoids, C. luteum and Ch. bifoveolatus, in the field. Understanding the parasitoid complex of S. frugiperda in Africa is also necessary before any development of classical biological controls involving the introduction of parasitoids from the Americas

Discovery pipelines for marine resources : an ocean of opportunity for biotechnology?

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under Grant agreement No 645884. CABI is an international intergovernmental organisation, and we gratefully acknowledge the core financial support from our member countries (and lead agencies) including the United Kingdom (Department for International Development), China (Chinese Ministry of Agriculture), Australia (Australian Centre for International Agricultural Research), Canada (Agriculture and Agri-Food Canada), Netherlands (Directorate-General for International Cooperation),and Switzerland (Swiss Agency for Development and Cooperation). See https://www.cabi.org/about-cabi/who-we-work-with/key-donors/ for full details.Marine microbial diversity offers enormous potential for discovery of compounds of crucial importance in healthcare, food security and bioindustry. However, access to it has been hampered by the difficulty of accessing and growing the organisms for study. The discovery and exploitation of marine bioproducts for research and commercial development requires state-of-the-art technologies and innovative approaches. Technologies and approaches are advancing rapidly and keeping pace is expensive and time consuming. There is a pressing need for clear guidance that will allow researchers to operate in a way that enables the optimal return on their efforts whilst being fully compliant with the current regulatory framework. One major initiative launched to achieve this, has been the advent of European Research Infrastructures. Research Infrastructures (RI) and associated centres of excellence currently build harmonized multidisciplinary workflows that support academic and private sector users. The European Marine Biological Research Infrastructure Cluster (EMBRIC) has brought together six such RIs in a European project to promote the blue bio-economy. The overarching objective is to develop coherent chains of high-quality services for access to biological, analytical and data resources providing improvements in the throughput and efficiency of workflows for discovery of novel marine products. In order to test the efficiency of this prototype pipeline for discovery, 248 rarely-grown organisms were isolated and analysed, some extracts demonstrated interesting biochemical properties and are currently undergoing further analysis. EMBRIC has established an overarching and operational structure to facilitate the integration of the multidisciplinary value chains of services to access such resources whilst enabling critical mass to focus on problem resolution.Publisher PDFPeer reviewe

Narrative approach to understand people's comprehension of acquaintance rape: The role of Sex Role Stereotyping

One of the most unreported crimes is acquaintance rape. This may be the result of people's understanding of what rape is because of their rape script and their stereotypes of victim characteristics. These judgements may be moderated by sex role stereotyping (SRS). We utilised a narrative approach to understand low and high SRS participants' rape scripts. Young-adult participants described what they believed a typical rape was, followed by describing an acquaintance rape and then what they believed the stereotypical victim of each crime would be. A narrative analysis was conducted on the data. We found that the blitz script is still held by 44% of low SRS and 47% of high SRS people despite 90% of rapes being committed by an acquaintance. While acquaintance rape scripts existed, the emotional imagery and content of these depended on participants level of SRS. Stereotypical victim characteristics also depended on SRS: those with high SRS were more likely to endorse rape myth ideals in describing victims than those with low SRS. These results have implications for educating people about what rape is so that victims might feel more confident in reporting rape

Steinernema unicornum sp. n. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode from Tierra del Fuego, Chile

A new species of entomopathogenic nematode,Steinernema unicornum sp. n., was isolated from a soil sample taken from the archipelago of Tierra del Fuego, southern Chile. The new species is characterised by a prominent, dorsally located, horn-like tooth on the cephalic extremity of the infective juvenile (a feature unique for the family); lateral field pattern of 2, 6, 8, 4 ridges; and morphometrics of the infective juvenile with body length of 732 (654-786) μm, excretory pore located 55 (48-62) μm posterior to the cephalic extremity, tail 60 (50-71) μm long, H% = 37 (24-49), E% = 91 (77-116) and a ratio = 25.4 (21.9-29.4). The first generation male has spicules 73 (60-82) μm long, gubernaculum 48 (44-52) μm long and SW% = 198 (184-217). The first generation female can be recognised by the presence of a series of transverse cuticular folds and ridges located immediately anterior and posterior to the vulva, the lack of a prominent post-anal swelling, and presence of a mucron-like projection on the tail tip. Sequences of the ITS and D2D3 regions of the ribosomal DNA confirm that S. unicornum sp. n. is a valid species

A highly-simplified and inexpensive MALDI-TOF mass spectrometry sample-preparation method with broad applicability to microorganisms, plants, and insects

Matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry prepares proteins intact in the gas phase with predominantly a single positive charge. The times-of-flight of charged proteins along a tube held at high vacuum after acceleration in an electrical field are proportional to the square root of the mass-over-charge ratios for the proteins, thereby allowing a mass spectrum to be generated, which can then be used to characterize or identify a protein-containing sample. Several sample-preparation methods are currently available but not all of these are applicable to some forms of fungal biomass and few of these are well suited to the analysis of plant or insect material. We have therefore developed a simplified method that: lyses cells, selectively solubilizes basic proteins, dissolves matrix to a suitable concentration, generates spectra with good intensity and peak richness, costs no more (and generally less) than current methods, and is not constrained in terms of throughput by the availability of centrifuges. Using this method, and a reagent formulation comprising α-cyano-4-hydroxycinnamic acid matrix close to saturation in 60%–65% (v/v) acetonitrile in water containing 2.5% (v/v) trifluoroacetic acid, we have been able to differentiate between strains for a representative subset of aflatoxin-producing and aflatoxin-non-producing strains of Aspergillus fungi, to differentiate between Indian and Pakistani strains of Himalayan balsam rust, to differentiate between closely-related Crassula spp. and regional biotypes of Crassula helmsii, and to differentiate between rubbervine introduced into Australia and Brazil. We have also analyzed fall armyworm and stem-borer samples stored in 70% (v/v) ethanol and old dried insect specimens.

Fungi in bottled water: a case study of a production plant

A one-year fungal survey of a water bottling plant was conducted in order to evaluate the incidence and fluctuations of the mycobiota. The dominant fungal genera in order of highest numbers isolated were Penicillium, Cladosporium and Trichoderma followed by Aspergillus, Paecilomyces, and others. As expected, the highest number of isolates were collected during the warmer months, particulary May and June. Indeed during these two months there were more fungi present in the water, indicating that during those times of the year when fungal contamination is high, 0,4 μm filters should be changed on a more regular basis. In order to assess whether contamination was single or multi-loci, molecular methods based on the PCR were used for Penicilium brevicompactum. Overall, fungal contamination arose from multiple sources. Some P. brevicompactum strains were very "alike" and were detected during different sampling times, indicating that they were endemic to the plant. There was no evidence to suggest that fungi detected in the source water passed through to other parts of the plant. However, there was evidence that fungal strains isolated from the water filter were detected elsewhere in the factory, confirming the need to change filters more regularly during periods of high fungal contamination. In order to improve quality control a HACCP programme was implemented and Best Practice Guidelines introduced.EC, CRAFT programme; Control of Mycological Contamination in Bottled Water (COMBOW)

Telenomus remus, a Candidate Parasitoid for the Biological Control of Spodoptera frugiperda in Africa, is already Present on the Continent

The fall armyworm, Spodoptera frugiperda, a moth originating from tropical and subtropical America, has recently become a serious pest of cereals in sub-Saharan Africa. Biological control offers an economically and environmentally safer alternative to synthetic insecticides that are being used for the management of this pest. Consequently, various biological control options are being considered, including the introduction of Telenomus remus, the main egg parasitoid of S. frugiperda in the Americas, where it is already used in augmentative biological control programmes. During surveys in South, West, and East Africa, parasitized egg masses of S. frugiperda were collected, and the emerged parasitoids were identified through morphological observations and molecular analyses as T. remus. The presence of T. remus in Africa in at least five countries provides a great opportunity to develop augmentative biological control methods and register the parasitoid against S. frugiperda. Surveys should be carried out throughout Africa to assess the present distribution of T. remus on the continent, and the parasitoid could be re-distributed in the regions where it is absent, following national and international regulations. Classical biological control should focus on the importation of larval parasitoids from the Americas.© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). The attached file is the published pdf