Centennial Hills Active Adult Center business plan

The economic indicators for the state of Nevada indicate a bleak financial future. The foreclosure rate of the Las Vegas Valley is five times the national average and the highest rate in the country (Hoak, 2010). Sales tax revenues continue spiraling down and fell by 6.6 percent in December 2009 from December 2008 (Garcia, 2010). State politicians face an estimated budget deficit of $3.5 billion. Local conditions are equally dire. The City of Las Vegas faces a budget shortfall of nearly$69 million for this fiscal year (Choate, 2010). City administrators struggle to find creative solutions to fill financial gaps and must make difficult decisions to reduce, consolidate or eliminate services and programs. The city’s Department of Leisure Services is no exception and administrators are examining ways to make fiscal cuts to the unit’s budget and increase efficiency. The department, which strives to bolster residents’ quality of life, provides services to all Las Vegans and has programs specifically targeting youth, seniors, and low-income families. The department has historically operated under what staff members describe as a social model, making decisions that maximize access without looking at data or efficiency. In light of current fiscal concerns, they hope to make the switch to a business enterprise model of operations that will maximize efficiency and allow for a focus on cost recovery that does not price out Las Vegans from utilizing services. To assist with city administrators’ aspirations to increase cost recovery throughout the department, this paper examines the fiscal and operational challenges at one of six Las Vegas senior centers in an effort to gauge the price elasticity of demand for services. Based on qualitative and quantitative data, the authors crafted seven recommendations to help the Centennial Hills Active Adult Center (CHAAC) create a business plan that allows for the cost recovery of at least 15% of its operational expenses. Those recommendations consist of the following: a change in pricing; increased emphasis on data collection and marketing; more efficient scheduling; an emphasis on seeking alternative funding; the development of a strategic plan for the center to include specific goals, objectives and performance measurements; a review of the center’s relationship to the YMCA; and increased transportation to and from the center. These recommendations will ensure the seniors of Las Vegas have an opportunity for a continuing relationship with the leisure services department. The authors hope this research may assist city officials in the months to come as they deliberate difficult decisions that will impact thousands of Las Vegas seniors and help administrators protect vital services

Principal component analysis and biochemical characterization of protein and starch reveal primary targets for improving sorghum grain

Limited progress has been made on genetic improvement of the digestibility of sorghum grain because of variability among different varieties. In this study, we applied multiple techniques to assess digestibility of grain from 18 sorghum lines to identify major components responsible for variability. We also identified storage proteins and enzymes as potential targets for genetic modification to improve digestibility. Results from principal component analysis revealed that content of amylose and total starch, together with protein digestibility (PD), accounted for 94% of variation in digestibility. Control of amylose content is understood and manageable. Up-regulation of genes associated with starch accumulation is clearly a future target for improving digestibility. To identify proteins that might be targets for future modification, meal from selected lines was digested in vitro with pancreatin in parallel with pepsin and α-amylase. The %PD was influenced by both the nature of the protein matrix and protein body packaging. Owing to its ability to form oligomers, the 20 kDa γ-kafirin was more resistant to digestion than counterparts lacking this ability, making it a target for down-regulation. Greater understanding of interactions among the three traits identified by principal component analysis is needed for both waxy and non-waxy varieties

A Comprehensive Transit Accessibility and Equity Dashboard

The TransitCenter Equity Dashboard tracks how well public transit systems in seven densely populated urban regions in the United States serve their riders and how changes to transit service affect riders over space, time, and cost constraints. The dashboard presents a series of charts and interactive maps that can be used to evaluate variations in transit accessibility and equity. It was created using publicly available data and primarily open-source software. All measures can be accessed by users seeking to conduct their own analyses. Results demonstrate differences in agency responses to COVID-19 as well as baseline transit service levels provided to different demographic groups

RNA-Seq analysis of resistant and susceptible potato varieties during the early stages of potato virus Y infection

Background: Potato virus Y (PVY) is one of the most important plant viruses affecting potato production. The interactions between potato and PVY are complex and the outcome of the interactions depends on the potato genotype, the PVY strain, and the environmental conditions. A potato cultivar can induce resistance to a specific PVY strain, yet be susceptible to another. How a single potato cultivar responds to PVY in both compatible and incompatible interactions is not clear. Results: In this study, we used RNA-sequencing (RNA-Seq) to investigate and compare the transcriptional changes in leaves of potato upon inoculation with PVY. We used two potato varieties: Premier Russet, which is resistant to the PVY strain O (PVYᴼ) but susceptible to the strain NTN (PVYᴺᵀᴺ), and Russet Burbank, which is susceptible to all PVY strains that have been tested. Leaves were inoculated with PVYᴼ or PVYᴺᵀᴺ, and samples were collected 4 and 10 h post inoculation (hpi). A larger number of differentially expressed (DE) genes were found in the compatible reactions compared to the incompatible reaction. For all treatments, the majority of DE genes were down-regulated at 4 hpi and up-regulated at 10 hpi. Gene Ontology enrichment analysis showed enrichment of the biological process GO term “Photosynthesis, light harvesting” specifically in PVYᴼ-inoculated Premier Russet leaves, while the GO term “nucleosome assembly” was largely overrepresented in PVYᴺᵀᴺ-inoculated Premier Russet leaves and PVYᴼ-inoculated Russet Burbank leaves but not in PVYᴼ-inoculated Premier Russet leaves. Fewer genes were DE over 4-fold in the incompatible reaction compared to the compatible reactions. Amongst these, five genes were DE only in PVYᴼ-inoculated Premier Russet leaves, and all five were down-regulated. These genes are predicted to encode for a putative ABC transporter, a MYC2 transcription factor, a VQ-motif containing protein, a non-specific lipid-transfer protein, and a xyloglucan endotransglucosylase-hydroxylase. Conclusions: Our results show that the incompatible and compatible reactions in Premier Russet shared more similarities, in particular during the initial response, than the compatible reactions in the two different hosts. Our results identify potential key processes and genes that determine the fate of the reaction, compatible or incompatible, between PVY and its host

Alternative Splicing in the Obligate Biotrophic Oomycete Pathogen Pseudoperonospora cubensis

Pseudoperonospora cubensis is an obligate pathogen and causative agent of cucurbit downy mildew. To help advance our understanding of the pathogenicity of P. cubensis, we used RNA-Seq to improve the quality of its reference genome sequence. We also characterized the RNA-Seq dataset to inventory transcript isoforms and infer alternative splicing during different stages of its development. Almost half of the original gene annotations were improved and nearly 4,000 previously unannotated genes were identified. We also demonstrated that approximately 24% of the expressed genome and nearly 55% of the intron-containing genes from P. cubensis had evidence for alternative splicing. Our analyses revealed that intron retention is the predominant alternative splicing type in P. cubensis, with alternative 5′- and alternative 3′-splice sites occurring at lower frequencies. Representatives of the newly identified genes and predicted alternatively spliced transcripts were experimentally validated. The results presented herein highlight the utility of RNA-Seq for improving draft genome annotations and, through this approach, we demonstrate that alternative splicing occurs more frequently than previously predicted. In total, the current study provides evidence that alternative splicing plays a key role in transcriptome regulation and proteome diversification in plantpathogenic oomycetes

Re-conceptualising talent management and development within the context of the low paid

Those working in organisations have choices to make not only associated with the goods and services they produce but also their wider social and economic impact. The number of employees in low skilled/low paid jobs and the high proportion of companies adopting business strategies based on low-specification goods and services are a concern for many developed and developing economies. Addressing this problem is not traditionally the concern of Human Resource Development however we argue that through exploring the role that a wider, more balanced approach to Sustainable Talent Management and Development (S-TMD) may play within the context of the low skilled in the UK provides a crucial link to enhancing an organisation’s performance and responsibility to society. At the heart of this approach lies a shift to appreciate the collective endeavour of work practices, an enhanced role for stakeholders and identification of, and participation in skills eco-systems to support sustainable development. The paper identifies the opportunity for S-TMD to move from a predominantly individualist, managerial and unitarist understanding to one grounded in the value of tacit and embedded development processes undertaken to reflect a pluralist, multi-voiced approach to understanding of a skills eco-system

Involvement of right dorsolateral prefrontal cortex in ill-structured design cognition: An fMRI study

In ill-structured tasks, the problem to be solved is poorly specified and there is no unique correct solution. Most evidence on brain mechanisms involved in dealing with such tasks comes from neuropsychology. Here, we developed an ill-structured design task suitable for testing in a functional neuroimaging environment and compared it with a matched well-structured problem-solving task using fMRI. Consistent with prior neuropsychological results, the design task was associated with greater activity in right dorsolateral prefrontal cortex compared with problem solving. This differential activity was specific to the problem studying phase rather than performance. Furthermore, the design and problem-solving tasks differed not only in overall levels of brain activity but also in patterns of functional interactions between brain regions. These results provide new evidence on the role of right dorsolateral prefrontal cortex in ill-structured situations, such as those involved in design cognition. Additionally, these results confirm the suitability of functional neuroimaging for studying such situations