DNA barcoding of moon jellyfish (Cnidaria, Scyphozoa, Ulmaridae, Aurelia): two cryptic species from the Azores (ne atlantic, macaronesia), and evaluation of the non-íIndigenous species (NIS)

Moon jellies are some of the most popular, widely distributed, and best-studied marine jellyfish. By the end of the past century only two or three Aurelia species were recognized, but with the rise of DNA barcoding studies, around thirty Aurelia species are presently accepted. Most of the species are morphologically indistinguishable and have restricted biogeography. We reveal, with COI, 16S, and ITS1-5.8S sequence data, two (pseudo-)cryptic species of Aurelia, potentially endemic to the Azores ecoregion, herein provisionally classified as A. "cf. pseudosolida" and A. "misteriosa". These species are closely related to the Mediterranean lineages of A. pseudosolida and A. persea, respectively. In the Azores, the shape of the campanula and oral arms readily distinguishes the two species: the former with folded oral arms and globose campanula, and the latter with flattened campanula and thick and long oral arms. Previous reports of A. solida and A. aurita in the Azores should generally correspond to A. "misteriosa" and A. cf. pseudosolida, respectively. The phylogenetic (re-)examination of the available DNA barcodes of Aurelia only evidenced human-mediated dispersal for A. coerulea, A. relicta, and A. aurita. Aurelia solida cannot be yet considered NIS in the Mediterranean. More jellyfish DNA (meta)barcoding should reveal further cryptic diversity, biological invasions, and phylogeographic inferences.Programa Operacional Açores 2020”, within the project “Águas-VivAz”–ref.: ACORES-01-0145- FEDER-000119info:eu-repo/semantics/publishedVersio

Influence of HOTAIR rs920778 and rs12826786 genetic variants on prostate cancer risk and progression-free survival

Aim: Evaluate the impact of the single nucleotide polymorphisms rs920778 and rs12826786 in the long noncoding RNA HOTAIR in the susceptibility and prognosis of prostate cancer (PCa) patients. Patients & methods: HOTAIR single nucleotide polymorphisms were genotyped by restriction fragment length polymorphism in 151 PCa cases and 180 cancer-free controls. Odds ratio, 95% CIs and prognostic significance were calculated. Results: Our data showed no statistically significant associations between HOTAIR polymorphic variants in rs920778 and rs12826786 and PCa susceptibility. However, the CC genotype in rs12826786 was significantly associated with shorter biochemical recurrence-free survival in pT3-stage PCa patients. Conclusion: Our results indicate that HOTAIR rs12826786 CC genotype may be an independent prognostic biomarker in a particular subset of PCa tumors.Fundação para a Ciência e Tecnologia (IF/00601/2012 to BM Costa; SFRH/BD/52287/2013 to AI Oliveira; SFRH/BD/88220/2012 to AX Magalhaes), Fundação Calouste Gulbenkian (BM Costa), Liga Portuguesa Contra o Cancro (BM Costa) and Inter-University Doctoral Programme in Ageing and Chronic Disease (PhDOC; to AI Oliveira and AX Magalhaes). Project co-financed by Programa Operacional Regional do Norte (ON.2-O Novo Norte), Quadro de Referencia Estratégico Nacional (QREN), Fundo Europeu de Desenvolvimento Regional (FEDER) and a grant (74-CI-IPOP) from Research Center of Portuguese Oncology Institute of Porto (C Jeronimo).info:eu-repo/semantics/publishedVersio

Diffusivities of metal acetylacetonates in liquid ethanol and comparison with the transport behavior in supercritical systems

Diffusion coefficients of chromium(III) acetylacetonate, palladium(II) acetylacetonate, nickel(II) acetylacetonate, vanadyl(II) acetylacetonate, and titanium(IV) oxyacetylacetonate in liquid ethanol were measured by chromatographic peak broadening method (CPB) over the range of temperature 303.15–333.15 K at atmospheric pressure. The dependencies of upon temperature and Stokes–Einstein coordinates were examined in detail. Moreover, the experimental data were modeled using nine equations from the literature to test their accuracy and prediction ability. The lower deviations were achieved by the 2-parameter correlation of Dymond-Hildebrand-Batschinski (0.32–2.17 %), the 1-parameter correlation of Tracer Liu-Silva-Macedo (1.58–3.88 %), and the 2-parameter correlations of Magalhães et al. (0.55–2.32 %). Finally, the proposed correlation based on hydrodynamic approach was found to well represent palladium(II) acetylacetonate and chromium(III) acetylacetonate systems in supercritical carbon dioxide and liquid ethanol, with the average absolute relative deviation of 3.15 and 5.02 %, respectively.publishe

Avaliação da função renal em ratos wistar com insuficiência renal

A incidência e a prevalência da doença renal crónica continuam a aumentar em todo o mundo. O modelo de redução de 5/6 de massa renal (5/6 RMR) é um dos procedimentos experimentais mais utilizados para a indução e o estudo das alterações associadas a uma insuficiência renal crónica. A resposta adaptativa do rim à redução cirúrgica do número de nefrónios parece ser suficientemente semelhante às características fisiopatológicas das nefropatias progressivas humanas. Neste estudo foram utilizados seis ratos machos da estirpe Wistar. Os animais foram anestesiados com xilazina (10 mg/kg) e ketamina (75 mg/kg). Em três animais foi realizada a redução de 5/6 de massa renal e nos restantes três a cirurgia foi simulada. Os animais foram divididos em dois grupos experimentais: grupo SO: animais em que a cirurgia foi simulada; e grupo RMR: animais submetidos à RMR. Foi realizada uma recolha de sangue e de urina no estado basal e depois mensalmente, durante 2 meses. Os animais submetidos à RMR apresentaram um aumento do fluxo urinário, um aumento das concentrações plasmáticas de ureia e de creatinina, uma diminuição da depuração de creatinina e um aumento das concentrações plasmáticas de potássio. Portanto, a redução cirúrgica de massa renal foi acompanhada por um declínio da função renal

Avaliação da função renal em ratos wistar com insuficiência renal

A incidência e a prevalência da doença renal crónica continuam a aumentar em todo o mundo. O modelo de redução de 5/6 de massa renal (5/6 RMR) é um dos procedimentos experimentais mais utilizados para a indução e o estudo das alterações associadas a uma insuficiência renal crónica. A resposta adaptativa do rim à redução cirúrgica do número de nefrónios parece ser suficientemente semelhante às características fisiopatológicas das nefropatias progressivas humanas. Neste estudo foram utilizados seis ratos machos da estirpe Wistar. Os animais foram anestesiados com xilazina (10 mg/kg) e ketamina (75 mg/kg). Em três animais foi realizada a redução de 5/6 de massa renal e nos restantes três a cirurgia foi simulada. Os animais foram divididos em dois grupos experimentais: grupo SO: animais em que a cirurgia foi simulada; e grupo RMR: animais submetidos à RMR. Foi realizada uma recolha de sangue e de urina no estado basal e depois mensalmente, durante 2 meses. Os animais submetidos à RMR apresentaram um aumento do fluxo urinário, um aumento das concentrações plasmáticas de ureia e de creatinina, uma diminuição da depuração de creatinina e um aumento das concentrações plasmáticas de potássio. Portanto, a redução cirúrgica de massa renal foi acompanhada por um declínio da função renal

Developing a Card Game for Assessment and Intervention in the Person and the Family in Palliative Care: “Pallium Game”

Abstract: Communication between the multidisciplinary team, the person, and the family in palliative and end-of-life situations implies, in most situations, a high negative emotional burden. Therefore, innovative strategies are needed to reduce it. The goal of this study is to describe the various stages of development and validation of a collaborative card game for people in palliative care and their families. Phase one is an exploratory study, Phase two is a Delphi study, and Phase three is a multiple case study. Participants for phases 2 and 3 were recruited using a convenience sampling method. The results demonstrate in an organized and structured way the different phases required to build a collaborative card game. The use of the game was found to be useful and effective. Four categories emerged from the content analysis of the open-ended responses: usability, evaluation tool, communication and therapeutic relationship, and meaning when using the game. A collaborative game in palliative care helps to create a space for individuals and families to express feelings and experiences, meeting the myriad of physical, psychosocial, and spiritual needs. The “Pallium game” is a useful and impactful approach to discussing sensitive topics in palliative care.info:eu-repo/semantics/publishedVersio

Influência da sacarose no desenvolvimento de cercárias das linhagens BH e SJ de Schistosoma mansoni

The development of cercariae from Schistosoma mansoni strains BH and SJ in Biomphalaria glabrata and Biomphalaria tenagophila treated with saccharose was studied. The molluscs were maintained in dechlorinated tap water containing 0.01% saccharose. After one week of treatment with saccharose, B. glabrata and B. tenagophila were exposed to ten S. mansoni miracidia, from BH and SJ strains respectively. Control snails of both species were maintained in dechlorinated tap water without saccharose and exposed to the same number of miracidia. There was no significant difference between the infection rates of snails treated or not with saccharose. However, the two groups of B. glabrata had significantly greater infection rates than the corresponding B. tenagophila groups. Molluscs treated with saccharose had a lower survival rate, with the greatest mortality occurring immediately before and at the beginning of cercariae release. Treatment with saccharose did not result in the release of more cercariae, but larvae from molluscs so treated showed a greater capacity to penetrate mouse skin, which was attributed to the greater energy supply during larval development in the mollusc.Foi estudado o desenvolvimento de cercárias de Schistosoma mansoni das linhagens BH e SJ em Biomphalaria glabrata e Biomphalaria tenagophila, respectivamente, tratadas com sacarose. Os moluscos foram mantidos em água declorada contendo 0,01% de sacarose. Após uma semana de tratamento com sacarose, B. glabrata e B. tenagophila foram expostas a dez miracídios de S. mansoni das linhagens BH e SJ, respectivamente. Moluscos controles de ambas as espécies mantidos em água declorada sem sacarose foram expostos ao mesmo número de miracídios. Não houve diferença significativa entre as taxas de infecção dos moluscos tratados ou não com sacarose. Entretanto, os dois grupos de B. glabrata tiveram taxas de infecção significativamente maiores que as correspondentes aos grupos de B. tenagophila. Moluscos tratados com sacarose apresentaram menor taxa de sobrevivência, com a maior mortalidade ocorrendo imediatamente depois e no início da liberação de cercárias. O tratamento com sacarose não resultou na maior liberação de cercárias, mas larvas de moluscos tratados com esse açúcar mostraram maior capacidade de penetrar na pele de camundongo, o que foi atribuído ao maior suprimento de energia durante o desenvolvimento larval no molusco.434

Suporte nutricional em diferentes unidades de cuidados intensivos

[resumo][abstract

Microscopic inspection of the adhesive interface of composite onlays after cementation on low loading: an in vitro study

Purpose: This study aimed to assess the layer thickness and microstructure of traditional resin-matrix cements and flowable resin-matrix composites at dentin and enamel to composite onlay interfaces after cementation on low loading magnitude. Materials and Methods: Twenty teeth were prepared and conditioned with an adhesive system for restoration with resin-matrix composite onlays manufactured by CAD-CAM. On cementation, tooth-to-onlay assemblies were distributed into four groups, including two traditional resin-matrix cements (groups M and B), one flowable resin-matrix composite (group G), and one thermally induced flowable composite (group V). After the cementation procedure, assemblies were cross-sectioned for inspection by optical microscopy at different magnification up to ×1000. Results: The layer thickness of resin-matrix cementation showed the highest mean values at around 405 µm for a traditional resin-matrix cement (group B). The thermally induced flowable resin-matrix composites showed the lowest layer thickness values. The resin-matrix layer thickness revealed statistical differences between traditional resin cement (groups M and B) and flowable resin-matrix composites (groups V and G) (p < 0.05). However, the groups of flowable resin-matrix composites did not reveal statistical differences (p < 0.05). The thickness of the adhesive system layer at around 7 µm and 12 µm was lower at the interfaces with flowable resin-matrix composites when compared to the adhesive layer at resin-matrix cements, which ranged from 12 µm up to 40 µm. Conclusions: The flowable resin-matrix composites showed adequate flowing even though the loading on cementation was performed at low magnitude. Nevertheless, significant variation in thickness of the cementation layer was noticed for flowable resin-matrix composites and traditional resin-matrix cements that can occur in chair-side procedures due to the clinical sensitivity and differences in rheological properties of the materials.info:eu-repo/semantics/publishedVersio