Food or friends? What motivates zebrafish (Danio rerio) performing a visual discrimination

This work was supported in part by the University of St Andrews’ QR block grant and the following grants awarded to CHB: NC3Rs G1000053; BBSRC BB/M007863; Leverhulme RPG-2016-143. CHB and VJB are Members of the Royal Society Industry Fellows’ College.As a model organism, zebrafish have much to offer neuroscientific research and they are increasingly being used in behavioral neuroscience, for example to study the genetics of learning and memory. As fish are often considered “less clever” than mammals, it is important to understand how they learn and to establish optimal testing conditions. In this study, we compared the efficacy of food reinforcement and social stimuli in supporting Pavlovian conditioning, Pavlovian-to-instrumental transfer, and acquisition of a two-alternative forced choice visual discrimination. Although equally effective in conditioning and in motivating discrimination learning, fish responded with shorter latencies when they were anticipating food but responded for a greater number of trials when anticipating the social stimulus. After learning, the reward was changed: food-reinforcement was replaced with the social stimulus and vice versa. Performance accuracy did not change, but response latency did: the group previously rewarded with food, but now rewarded with the social stimulus, showed a decrease in response vigor. This is a negative contrast effect, which is well established in rats, but was thought to be absent in fish because they lacked goal representation. Our results show that zebrafish, like rats, do have goal representations. Furthermore, we have shown that whereas food has greater incentive salience than social stimuli, fish become satiated rapidly, but motivation to seek social stimuli is sustained. We conclude that zebrafish are well motivated by a mixed economy of social stimuli and food.PostprintPeer reviewe

The quark mass and μ\mu dependence of the QCD chiral critical point

In order to study the QCD chiral critical point we investigate Binder Cumulants of the chiral condensate. The results were obtained from simulations of 3 and 2+1 flavors of standard staggered fermions and 3 flavors of p4 improved staggered fermions. The quark masses used are close to the physical quark mass. To extract the dependence on quark mass and chemical potential we apply a new reweighting technique based on a Taylor expansion of the action. The reweighting accuracy is ${\cal O}(m)$ for the standard and ${\cal O}(m^2)$, ${\cal O}(\mu^2)$ for the p4 action.Comment: 3 pages, 6 figures, Lattice2002(nonzerot

Saturation from nuclear pion dynamics

We construct an equation-of-state for nuclear matter based on the chiral Lagrangian. The relevant scales are discussed and an effective chiral power expansion scheme, which is constructed to work around the nuclear saturation density, is presented. A realistic equation-of-state is obtained by adjusting one free parameter, when the leading and subleading terms in the expansion are included. The saturation mechanism is due to correlations induced by the one-pion-exchange interaction. Furthermore, we find a substantial deviation from the Fermi-gas estimate of the quark condensate in nuclear matter already at the saturation density.Comment: revised version, with minor corrections, 13 pages, 3 Postscript figure

Fragments of the earliest land plants

The earliest fossil evidence for land plants comes from microscopic dispersed spores. These microfossils are abundant and widely distributed in sediments, and the earliest generally accepted reports are from rocks of mid-Ordovician age (Llanvirn, 475 million years ago). Although distribution, morphology and ultrastructure of the spores indicate that they are derived from terrestrial plants, possibly early relatives of the bryophytes, this interpretation remains controversial as there is little in the way of direct evidence for the parent plants. An additional complicating factor is that there is a significant hiatus between the appearance of the first dispersed spores and fossils of relatively complete land plants (megafossils): spores predate the earliest megafossils (Late Silurian, 425 million year ago) by some 50 million years. Here we report the description of spore-containing plant fragments from Ordovician rocks of Oman. These fossils provide direct evidence for the nature of the spore-producing plants. They confirm that the earliest spores developed in large numbers within sporangia, providing strong evidence that they are the fossilized remains of bona fide land plants. Furthermore, analysis of spore wall ultrastructure supports liverwort affinities

Technical note: successful DNA amplification of DNA from non-destructive buccal swabbing in Vespertilionid and Rhinolophid bats

\ua9 The Author(s) 2024.Acquiring DNA from wild bats (Mammalia: Chiroptera) is typically undertaken utilizing highly invasive (but non-lethal) sampling techniques comprising wing biopsies and occasional blood samples. While non-invasive sampling is possible through the extraction of DNA from faecal samples, it is not always possible to acquire samples from individual bats whilst conducting fieldwork, and as such, this method is primarily applicable to roost occupancy identification. Similarly, wing swabbing is liable to cross-contamination from roost mates. Here we present the first use of oral (buccal) swabbing for successful, species-resolution DNA sequencing of Vespertilionidae and Rhinolophidae in 10 bat species (nine Vespertilionidae and one Rhinolophidae) from the UK

The Chiral Critical Point in 3-Flavour QCD

We determine the second order endpoint of the line of first order phase transitions, which occur in the light quark mass regime of 3-flavour QCD at finite temperature, and analyze universal properties of this chiral critical point. A detailed analysis of Binder cumulants and the joint probability distributions of energy like and ordering-field like observables confirms that the chiral critical point belongs to the universality class of the three dimensional Ising model. From a calculation with improved gauge and staggered fermion actions we estimate that the transition is first order for pseudo-scalar meson masses less than about 200 MeV.Comment: 16 pages, LaTeX2e File, 7 EPS-file

Multiscale Petrov-Galerkin method for high-frequency heterogeneous Helmholtz equations

This paper presents a multiscale Petrov-Galerkin finite element method for time-harmonic acoustic scattering problems with heterogeneous coefficients in the high-frequency regime. We show that the method is pollution free also in the case of heterogeneous media provided that the stability bound of the continuous problem grows at most polynomially with the wave number k. By generalizing classical estimates of Melenk (Ph.D. Thesis 1995) and Hetmaniuk (Commun. Math. Sci. 5, 2007) for homogeneous medium, we show that this assumption of polynomially wave number growth holds true for a particular class of smooth heterogeneous material coefficients. Further, we present numerical examples to verify our stability estimates and implement an example in the wider class of discontinuous coefficients to show computational applicability beyond our limited class of coefficients

MicroRNA expression in lymphocyte development and malignancy

This article is available open access through the publisher’s website. Copyright @ 2008 Macmillan Publishers Limited.No abstract available.The Leukemia Research Fund, the Julian Starmer-Smith Memorial Fund, and the Medical Research Council

Variation in advanced stage at diagnosis of lung and female breast cancer in an English region 2006-2009

This is the final published version. Available from Springer Nature via the DOI in this record.Background: Understanding variation in stage at diagnosis can inform interventions to improve the timeliness of diagnosis for patients with different cancers and characteristics. Methods: We analysed population-based data on 17 836 and 13 286 East of England residents diagnosed with (female) breast and lung cancer during 2006-2009, with stage information on 16 460 (92%) and 10 435 (79%) patients, respectively. Odds ratios (ORs) of advanced stage at diagnosis adjusted for patient and tumour characteristics were derived using logistic regression. Results :We present adjusted ORs of diagnosis in stages III/IV compared with diagnosis in stages I/II. For breast cancer, the frequency of advanced stage at diagnosis increased stepwise among old women (ORs: 1.21, 1.46, 1.68 and 1.78 for women aged 70-74, 75-79, 80-84 and ≥85, respectively, compared with those aged 65-69, P<0.001). In contrast, for lung cancer advanced stage at diagnosis was less frequent in old patients (ORs: 0.82, 0.74, 0.73 and 0.66, P<0.001). Advanced stage at diagnosis was more frequent in more deprived women with breast cancer (OR: 1.23 for most compared with least deprived, P=0.002), and in men with lung cancer (OR: 1.14, P=0.011). The observed patterns were robust to sensitivity analyses approaches for handling missing stage data under different assumptions. Conclusion: Interventions to help improve the timeliness of diagnosis of different cancers should be targeted at specific age groups. © 2012 Cancer Research UK All rights reserved