Pragas e dano em milho adubado com remineralizador de solo

Objetivou-se avaliar a incidência de pragas em milho adubado com remineralizador de solo oriundo de rocha silicatada em comparação à adubação química com NPK. O experimento foi conduzido em área experimental no Instituto Federal do Norte de Minas Gerais, Campus Januária, Minas Gerais, com o cultivo de milho de ciclo médio, em delineamento em blocos casualizados, com seis tratamentos, quatro repetições, quatro linhas de plantio/parcela. Os tratamentos consistiram no fornecimento de 0, 2, 4, 8, 16 t ha-1 de remineralizador (pó de rocha NaturalPlus® de Ipirá, Bahia) e NPK em linha de plantio. Vinte plantas da área útil de cada parcela foram monitoradas semanalmente para incidência de pragas durante os estágios fenológicos V1 a V12. No estágio reprodutivo R3 avaliou-se o dano causado pela lagarta do cartucho em folhas por meio de escala de dano visual. Durante o levantamento observou-se a ocorrência de Spodoptera frugiperda, Dalbulus maidis e Diabrotica speciosa em menor quantidade nos tratamentos com remineralizador de solo, independente da dose. O dano causado pela lagarta do cartucho na redução da área foliar foi diretamente proporcional ao número de lagartas. O remineralizador de rocha silicatada reduziu a incidência de insetos sugadores e o dano causado por lagarta do cartucho na cultura do milho

Neurostimulation Combined With Cognitive Intervention in Alzheimer’s Disease (NeuroAD): Study Protocol of Double-Blind, Randomized, Factorial Clinical Trial

Despite advances in the treatment of Alzheimer’s disease (AD), there is currently no prospect of a cure, and evidence shows that multifactorial interventions can benefit patients. A promising therapeutic alternative is the use of transcranial direct current stimulation (tDCS) simultaneously with cognitive intervention. The combination of these non-pharmacological techniques is apparently a safe and accessible approach. This study protocol aims to compare the efficacy of tDCS and cognitive intervention in a double-blind, randomized and factorial clinical trial. One hundred participants diagnosed with mild-stage AD will be randomized to receive both tDCS and cognitive intervention, tDCS, cognitive intervention, or placebo. The treatment will last 8 weeks, with a 12-month follow-up. The primary outcome will be the improvement of global cognitive functions, evaluated by the AD Assessment Scale, cognitive subscale (ADAS-Cog). The secondary outcomes will include measures of functional, affective, and behavioral components, as well as a neurophysiological marker (Brain-derived neurotrophic factor, BDNF). This study will enable us to assess, both in the short and long term, whether tDCS is more effective than the placebo and to examine the effects of combined therapy (tDCS and cognitive intervention) and isolated treatments (tDCS vs. cognitive intervention) on patients with AD.Clinical Trial Registration: www.ClinicalTrials.gov, identifier NCT02772185—May 5, 2016

Sandfly fauna (Diptera: Psychodidae) in an urban area, Central-West of Brazil

ABSTRACT Biological and ecological relations among vectors and their pathogens are important to understand the epidemiology of vector-borne diseases. Camapuã is an endemic area for visceral and tegumentary leishmaniasis. The aim of this study was to characterize the sandfly fauna present in Camapuã , MS, Brazil. Sand flies were collected every fortnight from May 2014 to April 2015 using automatic light traps in the domicile and peridomicile of twelve neighborhoods and forest. The collected specimens were identified based on morphology according to the valid identification keys. In total, 2005 sandflies of five genera and nine species were collected. Nyssomyia whitmani and Lutzomyia cruzi were the most abundant species. Males were more abundant, with a male-to-female ratio of 2.14. The highest diversity was observed in peripheral neighborhood, with abundant plant cover. The peridomicile presented greater abundance of sandflies, with the predominance of Ny. whitmani . No significant correlation between the absolute frequencies of the most abundant species and the precipitation variable was observed; however, there was a predominance of Lu. cruzi in the rainy season. We observed a high frequency of sandflies in urban area, especially vector species. The presence of Nyssomyia whitmani and Lutzomyia cruzi indicate the necessity for health surveillance in the municipality. Additional method of collection such as sticky trap is also recommended for appropriate faunestic study

The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability

Chromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. Its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) ≈500 ORFs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) wide-spread utilization of quorum sensing for control of inducible systems, all of which underpin the versatility and adaptability of the organism. The genome also contains extensive but incomplete arrays of ORFs coding for proteins associated with mammalian pathogenicity, possibly involved in the occasional but often fatal cases of human C. violaceum infection. There is, in addition, a series of previously unknown but important enzymes and secondary metabolites including paraquat-inducible proteins, drug and heavy-metal-resistance proteins, multiple chitinases, and proteins for the detoxification of xenobiotics that may have biotechnological applications

Catálogo Taxonômico da Fauna do Brasil: setting the baseline knowledge on the animal diversity in Brazil

The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Pragas e dano em milho adubado com remineralizador de solo

The objective of this study was to evaluate the incidence of pests in maize fertilized with soil remineralizer from silicate rock compared to chemical fertilization with NPK. The experiment was conducted in an experimental area at the Federal Institute of Northern Minas Gerais, Campus Januária, Minas Gerais, with the cultivation of medium - cycle corwith corn grain medium-cycle in a randomized block design, with six treatments, four replications, four planting / plot lines . The treatments consisted in the supply of 0, 2, 4, 8, 16 t ha-1 remineralizer (NaturalPlus® rock powder from Ipirá, Bahia) and NPK in planting line. Twenty plants of the area of each plot were monitored weekly for pest incidence during the phenological stages V1 to V12. In the reproductive stage R3 the damage caused by the caterpillar in leaves was evaluated by means of scale of visual damage. During the survey, the occurrence of Spodoptera frugiperda, Dalbulus maidis and Diabrotica speciosa was observed in a smaller quantity in the treatments with soil remineralizer, regardless of the dose. The damage caused by the caterpillar on leaf area reduction was directly proportional to the number of caterpillars. The silicate rock remineralizer reduced the incidence of sucking insects and damage caused by caterpillars in corn.Objetivou-se avaliar a incidência de pragas em milho adubado com remineralizador de solo oriundo de rocha silicatada em comparação à adubação química com NPK. O experimento foi conduzido em área experimental no Instituto Federal do Norte de Minas Gerais, Campus Januária, Minas Gerais, com o cultivo de milho de ciclo médio, em delineamento em blocos casualizados, com seis tratamentos, quatro repetições, quatro linhas de plantio/parcela. Os tratamentos consistiram no fornecimento de 0, 2, 4, 8, 16 t ha-1 de remineralizador (pó de rocha NaturalPlus® de Ipirá, Bahia) e NPK em linha de plantio. Vinte plantas da área útil de cada parcela foram monitoradas semanalmente para incidência de pragas durante os estágios fenológicos V1 a V12. No estágio reprodutivo R3 avaliou-se o dano causado pela lagarta do cartucho em folhas por meio de escala de dano visual. Durante o levantamento observou-se a ocorrência de Spodoptera frugiperda, Dalbulus maidis e Diabrotica speciosa em menor quantidade nos tratamentos com remineralizador de solo, independente da dose. O dano causado pela lagarta do cartucho na redução da área foliar foi diretamente proporcional ao número de lagartas. O remineralizador de rocha silicatada reduziu a incidência de insetos sugadores e o dano causado por lagarta do cartucho na cultura do milho

Effects of eating disorders on oral fungal diversity

Background. The eating disorders anorexia and bulimia nervosa can cause several systemic and oral alterations related to poor nutrition and induced vomiting; however, the oral microflora of these patients is poorly studied. Objective. The aim of this study was to evaluate fungal microflora in the oral cavity of these patients by culture-dependent and culture-independent methods. Study Design. Oral rinse samples were cultured to assess the prevalence of Candida species, and the isolates were identified by API system. Microorganism counts were compared by the Mann-Whitney test (5%). Ribotyping, a type of molecular analysis, was performed by sequencing the D1/D2 regions of 28S rRNA. Results. Our results demonstrated that the eating disorder group showed higher oral Candida spp. prevalence with culture-dependent methods and higher species diversity with culture-independent methods. Conclusions. Eating disorders can lead to an increased oral Candida carriage. Culture-independent identification found greater fungal diversity than culture-dependent methods. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;113:512-517)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [06/60851-0, 07/50350-7]Fundacao de Amparo a Pesquisa do Estado de Sao Paul

Urban pollution greatly enhances formation of natural aerosols over the Amazon rainforest.

One of the least understood aspects in atmospheric chemistry is how urban emissions influence the formation of natural organic aerosols, which affect Earth's energy budget. The Amazon rainforest, during its wet season, is one of the few remaining places on Earth where atmospheric chemistry transitions between preindustrial and urban-influenced conditions. Here, we integrate insights from several laboratory measurements and simulate the formation of secondary organic aerosols (SOA) in the Amazon using a high-resolution chemical transport model. Simulations show that emissions of nitrogen-oxides from Manaus, a city of ~2 million people, greatly enhance production of biogenic SOA by 60-200% on average with peak enhancements of 400%, through the increased oxidation of gas-phase organic carbon emitted by the forests. Simulated enhancements agree with aircraft measurements, and are much larger than those reported over other locations. The implication is that increasing anthropogenic emissions in the future might substantially enhance biogenic SOA in pristine locations like the Amazon

Bullying: desafíos para a convivencia ética no combate ao assedio

Este artigo registra parte dos resultados de uma atividade de extensão universitária realizada junto a trabalhadores na educação e saúde do município de Humaitá, sul do Estado do Amazonas, onde foram realizadas atividades de reflexão-ação acerca da convivência ética e democrática. Está organizado em duas partes: a primeira destaca aspectos conceituais e filosóficos acerca do tema; a segunda parte apresenta os resultados da intervenção em contextos da educação e da saúde