Chikungunya Occurrence among a Religious Missions Trip to Haiti in the Summer of 2014 and Implications for Community Health

As global travel becomes more common, the risk of spreading infectious diseases is increasing. Vectors too are spreading and becoming invasive species in environmental niches where they had previously been absent. The potential continues to grow for people to bring back diseases and infect local naive insect populations, which over time will increase the likelihood of the disease transmission occurring locally. This article evaluates the impact of a mission’s trip to Haiti and the return to a region of the United States that just recently became invaded by Aedes aegypti. The attack rate among the missionaries was approximately 20% (n=2), but this brings into question precautions that missionaries and other travelers will want to take upon return from tropical regions with endemic vector borne disease. Providers as well as travel clinics should educate patients regarding their viremic period and the potential to bring back diseases with them. While it would take hundreds of people to be viremic and to be bitten by the naive vector to establish local transmission, the preventative action is minor and would require travelers from endemic areas to continue to apply insect repellant for 7 days after return from an endemic region

Creating an Organization to Support SFA’s Women Employees

The purpose of this session is two-fold. First, we wish to introduce the SFA OWLE (Organization for Women’s Leadership and Equity), the newly created professional women’s organization, to interested members of our campus community. Second, we wish to share experiences that we believe are relevant to others on our campus, in our community, and at other institutions, who might seek to create organizations through which to advocate for the unique needs of their marginalized or underrepresented group. To that end, this session will include a panel of female employees at SFA who have worked for more than a year to plan for, create, and implement a professional women’s organization for our campus. The panel will begin the session by sharing the reasons why a professional women’s organization is needed on our campus (as well as many similar institutions nationwide) and will describe the steps taken to create the organization. The committees constituted in the organization’s by-laws reflect the challenges faced by women in academia. We will share challenges we faced as we sought to create an organization that would provide support to women of differing types of employment (e.g., both staff and faculty), from different academic disciplines and areas of the university, in different stages of their personal and professional lives, and from diverse positionalities (i.e., race, ethnicity, gender, sexual orientation, parenthood status, etc.). We will also identify some of the successes our organization has achieved in its early stages

LC-MS proteomics analysis of the iInsulin/IGF-1-deficient Caenorhabditis elegans daf-2(e1370) mutant reveals extensive restructuring of intermediary metabolism

The insulin/IGF-1 receptor is a major known determinant of dauer formation, stress resistance, longevity, and metabolism in Caenorhabditis elegans. In the past, whole-genome transcript profiling was used extensively to study differential gene expression in response to reduced insulin/IGF-1 signaling, including the expression levels of metabolism-associated genes. Taking advantage of the recent developments in quantitative liquid chromatography mass spectrometry (LC-MS)-based proteomics, we profiled the proteomic changes that occur in response to activation of the DAF-16 transcription factor in the germline-less glp-4(bn2);daf-2(e1370) receptor mutant. Strikingly, the daf-2 profile suggests extensive reorganization of intermediary metabolism, characterized by the upregulation of many core intermediary metabolic pathways. These include glycolysis/gluconeogenesis, glycogenesis, pentose phosphate cycle, citric acid cycle, glyoxylate shunt, fatty acid beta-oxidation, one-carbon metabolism, propionate and tyrosine catabolism, and complexes I, II, III, and V of the electron transport chain. Interestingly, we found simultaneous activation of reciprocally regulated metabolic pathways, which is indicative of spatiotemporal coordination of energy metabolism and/or extensive post-translational regulation of these enzymes. This restructuring of daf-2 metabolism is reminiscent to that of hypometabolic dauers, allowing the efficient and economical utilization of internal nutrient reserves and possibly also shunting metabolites through alternative energy-generating pathways to sustain longevity

Global Proteomic Analysis of Two Tick-Borne Emerging Zoonotic Agents: Anaplasma Phagocytophilum and Ehrlichia Chaffeensis

Anaplasma phagocytophilum and Ehrlichia chaffeensis are obligatory intracellular α-proteobacteria that infect human leukocytes and cause potentially fatal emerging zoonoses. In the present study, we determined global protein expression profiles of these bacteria cultured in the human promyelocytic leukemia cell line, HL-60. Mass spectrometric (MS) analyses identified a total of 1,212 A. phagocytophilum and 1,021 E. chaffeensis proteins, representing 89.3 and 92.3% of the predicted bacterial proteomes, respectively. Nearly all bacterial proteins (≥99%) with known functions were expressed, whereas only approximately 80% of “hypothetical” proteins were detected in infected human cells. Quantitative MS/MS analyses indicated that highly expressed proteins in both bacteria included chaperones, enzymes involved in biosynthesis and metabolism, and outer membrane proteins, such as A. phagocytophilum P44 and E. chaffeensis P28/OMP-1. Among 113 A. phagocytophilum p44 paralogous genes, 110 of them were expressed and 88 of them were encoded by pseudogenes. In addition, bacterial infection of HL-60 cells up-regulated the expression of human proteins involved mostly in cytoskeleton components, vesicular trafficking, cell signaling, and energy metabolism, but down-regulated some pattern recognition receptors involved in innate immunity. Our proteomics data represent a comprehensive analysis of A. phagocytophilum and E. chaffeensis proteomes, and provide a quantitative view of human host protein expression profiles regulated by bacterial infection. The availability of these proteomic data will provide new insights into biology and pathogenesis of these obligatory intracellular pathogens

Quarantine Mothering and Working at Home: How Institutions of Higher Education Supported (or Failed to Support) Academic Mothers

This mixed methods study explores whether and how explicit policies, implicit practices, and internal communication from university administrators about aca-demic mothers’ work lives and expectations were impacted by the 2020 COVID-19 quarantine protocols. As this was a large study focussing on university policies addressing the presence of children on campus and the ways in which their enforcement or nonenforcement affected the personal and professional lives of faculty, we used purposive sampling (Palys) and snowball sampling (Patton) to distribute a survey in academic social media groups and to professional organization listservs (Palys). Among other things, the survey asked participants to report how well they thought their university was handling the COVID-19 pandemic and invited them to participate in an in-depth interview. As a result of the survey responses, we subsequently interviewed nineteen academic mothers from a range of academic disciplines, ages, and types of institutions, until we reached theoretical saturation (Strauss and Corbin). The semi-structured interview protocol included questions about the impact of COVID-19-related policies, practices, and messaging regarding children on participants’ job satisfaction, mental and physical health, as well as work-life balance. We used open and axial coding (Strauss and Corbin) and the constant comparative method (Glaser and Strauss) to analyze the data. We then triangulated the data by comparing interview and survey findings, engaging multiple researchers in the analysis, and conducting peer debriefings (Denzin and Lincoln; Lincoln and Guba). Findings highlight institutional policies and practices that serve or fail to serve faculty in terms of supporting their professional advancement in teaching, research, and service

Favorite Teacher or Worst Nightmare? Teachers who Crossed the Line

What kind of teacher sexually abuses her students? The current study, guided by critical theory, examines common characteristics of female high school teachers who sexually abuse their students. Extant research on teacher student relationships have found commonalities in the demographics of the offenders, their experience of adverse childhood experiences, common age of the victims, re-offense rates, and public perception of these types of crimes. The current study furthers previous research, by investigating their marital status at the time of the offense (married or not married), whether or not the offender has children, and school type (public or private). This study collected n=18 cases of female high school teachers in the state of Florida who are sexual offenders, and whose victims are their students. We have collected qualitative secondary data from news articles and investigative reports published by two websites: The Canadian Children’s Rights Council and The South Florida Sun Sentinel. Specific characteristics on each of the offenders were coded and inputted on Excel and data was analyzed using the phenomenological methodology. These characteristics include our three main constructs, in addition to offender as well as victim age. Results of the study will be discussed to help illuminate further common characteristics among these offenders, especially focusing on their relationship status, parental status, and school type in which they were employed at the time of the offense. Finally, implications on future research will be discussed

Using Pen-Side Measurable Blood Parameters to Predict or Identify Dystocic Lambing Events

Dystocia is the greatest contributor to neonatal lamb mortality in Australia and poses significant welfare and economic concerns worldwide. In this study, we set out to investigate whether pen-side analysis technology could be employed to detect blood parameters predictive of dystocic labour events in sheep. In a pilot trial, we collected and analysed blood samples in pen-side assays for glucose, lactate, pH, pCO2, pO2, base excess, HCO3, TCO2, sO2, lactate, sodium, potassium, chloride, calcium, urea nitrogen, creatinine, haematocrit, haemoglobin and anion gap. From the pilot data, we identified creatinine, TCO2, chloride and calcium as potentially useful markers. To develop a time course and to establish variability of the selected blood parameters, a time series of samples was collected from 12 ewes, from mid-gestation to 48 h after birth. For the main trial, blood samples were collected at mid- and late gestation for glucose determination and for the full set of blood parameters at three time points before, at and after birth. Possible predictors of lambing difficulty were chloride, haematocrit and haemoglobin, sampled one week before birth; creatinine, sampled at birth; and blood pH and base excess after birth. In conclusion, we found that pen-side analysis of blood markers showed promise in identifying dystocic lambing events

Water quality impacts and river system recovery following the 2014 Mount Polley mine tailings dam spill, British Columbia, Canada

The Mount Polley mine tailings embankment breach on August 4th 2014, in British Columbia, Canada, is the second largest mine waste spill on record. The mine operator responded swiftly by removing significant quantities of tailings from the primary receiving watercourse, stabilizing the river corridor and beginning construction of a new river channel. This presented a unique opportunity to study spatial patterns of element cycling in a partially-restored and alkaline river system. Overall, water quality impacts are considered low with Cu, and to a lesser extent V, being the only elements of concern. However, the spatial pattern of stream Cu loading suggested chemical (dominant at low flow) and physical (dominant at high flow) mobilization processes operating in different parts of the watershed. Chemical mobilization was hypothesized to be due to Cu sulfide (chalcopyrite) oxidation in riparian tailings and reductive dissolution of Cu-bearing Fe oxides in tailings and streambed sediments whereas physical mobilization was due to erosion and suspension of Cu-rich stream sediments further downstream. Although elevated aqueous Cu was evident in Hazeltine Creek, this is considered a relatively minor perturbation to a watershed with naturally elevated stream Cu concentrations. The alkaline nature of the tailings and the receiving watercourse ensures most aqueous Cu is rapidly complexed with dissolved organic matter or precipitates as secondary mineral phases. Our data highlights how swift removal of spilled tailings and river corridor stabilization can limit chemical impacts in affected watersheds but also how chemical mobilization (of Cu) can still occur when the spilled tailings and the receiving environment are alkaline. We present a conceptual model of Cu cycling in the Hazeltine Creek watershed.publishersversionPeer reviewe

Origin and Fate of Vanadium in the Hazeltine Creek Catchment following the 2014 Mount Polley Mine Tailings Spill in British Columbia, Canada

This is the final version. Available on open access from American Chemical Society via the DOI in this recordResults are presented from the analysis of aqueous and solid-phase V speciation within samples collected from the Hazeltine Creek catchment affected by the August 2014 Mount Polley mine tailings dam failure, Canada. Electron microprobe and XANES analysis found that V is present as V3+ substituted into magnetite, and V3+ and V4+ substituted into titanite, both of which occur in the spilled Mount Polley tailings. Secondary Fe oxyhydroxides forming in inflow waters and on creek beds have V K-edge XANES spectra exhibiting E½ positions and pre-edge features consistent with the presence of V5+ species, suggesting sorption of this species on these secondary phases. PHREEQC modelling suggests that the stream waters mostly contain V5+, and the inflow and pore waters contain a mixture of V3+ and V5+. These data, and stream, inflow and pore water chemical data, suggest that dissolution of V(III)-bearing magnetite, V(III,IV)-bearing titanite, V(V)-bearing Fe(-Al-Si-Mn) oxhydroxides, V-bearing Al(OH)3 and/or -clay minerals may have occurred. In the circumneutral pH environment of Hazeltine Creek elevated V concentrations are likely naturally attenuated by formation of V(V)-bearing secondary Fe oxyhydroxide, Al(OH)3 or clay mineral colloids, suggesting that the V is not bioavailable. A conceptual model is presented describing the origin and fate of V in Hazeltine Creek that is applicable to other river systems.Natural Environment Research Council (NERC

Determining the Contribution of Epidermal Cell Shape to Petal Wettability Using Isogenic Antirrhinum Lines

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited