13 research outputs found
High throughput development of a non protein A monoclonal antibody purification process using mini-columns and bio-layer interferometry
No full textHigh throughput screening of mixed-mode sorbents and optimisation using pre-packed lab-scale columns for the purification of the recombinant allergen rBet v 1a
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Radial scale-down of packed bed chromatography in a thin cylindrical tube for preparative media
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Trend analysis of performance parameters of pre-packed columns for protein chromatography over a time span of ten years
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Evaluation of mixed-mode chromatographic resins for separating IgG from serum albumin containing feedstock
No full textMixed Mode Chromatography, Complex Development for Large Opportunities
No full textMixed mode chromatography resins with salt tolerance, large design space and orthogonal selectivity requires a slightly more complex development than traditional resins. It is important to screen several ligands and several binding and elution conditions. This allows taking full advantage of these resins. High-Throughput Screening (HTS) for Process Development should be done with the help of Design of Experiment (DoE). It could be performed in filter plates or Robocolumns, and assisted by liquid handling automated workstation. Modeling of the results allows the choice of optimal parameters that can then be validated and scaled up. All this leads to a better knowledge and robustness of the purification step
