209 research outputs found
Linear -positive sets and their polar subspaces
In this paper, we define a Banach SNL space to be a Banach space with a
certain kind of linear map from it into its dual, and we develop the theory of
linear -positive subsets of Banach SNL spaces with Banach SNL dual spaces.
We use this theory to give simplified proofs of some recent results of
Bauschke, Borwein, Wang and Yao, and also of the classical Brezis-Browder
theorem.Comment: 11 pages. Notational changes since version
Quantum Sign Permutation Polytopes
Convex polytopes are convex hulls of point sets in the -dimensional space
\E^n that generalize 2-dimensional convex polygons and 3-dimensional convex
polyhedra. We concentrate on the class of -dimensional polytopes in \E^n
called sign permutation polytopes. We characterize sign permutation polytopes
before relating their construction to constructions over the space of quantum
density matrices. Finally, we consider the problem of state identification and
show how sign permutation polytopes may be useful in addressing issues of
robustness
Excited States of Proton-bound DNA/RNA Base Homo-dimers: Pyrimidines
We are presenting the electronic photo fragment spectra of the protonated
pyrimidine DNA bases homo-dimers. Only the thymine dimer exhibits a well
structured vibrational progression, while protonated monomer shows broad
vibrational bands. This shows that proton bonding can block some non radiative
processes present in the monomer.Comment: We acknowledge the use of the computing facility cluster GMPCS of the
LUMAT federation (FR LUMAT 2764
An additive subfamily of enlargements of a maximally monotone operator
We introduce a subfamily of additive enlargements of a maximally monotone
operator. Our definition is inspired by the early work of Simon Fitzpatrick.
These enlargements constitute a subfamily of the family of enlargements
introduced by Svaiter. When the operator under consideration is the
subdifferential of a convex lower semicontinuous proper function, we prove that
some members of the subfamily are smaller than the classical
-subdifferential enlargement widely used in convex analysis. We also
recover the epsilon-subdifferential within the subfamily. Since they are all
additive, the enlargements in our subfamily can be seen as structurally closer
to the -subdifferential enlargement
Photophysics of Isolated Rose Bengal Anions
Dye molecules based on the xanthene moiety are widely used as fluorescent probes in bioimaging and technological applications due to their large absorption cross-section for visible light and high fluorescence quantum yield. These applications require a clear understanding of the dyeâs inherent photophysics and the effect of a condensed-phase environment. Here, the gas-phase photophysics of the rose bengal doubly deprotonated dianion [RB â 2H]2â, deprotonated monoanion [RB â H]â, and doubly deprotonated radical anion [RB â 2H]âąâ is investigated using photodetachment, photoelectron, and dispersed fluorescence action spectroscopies, and tandem ion mobility spectrometry (IMS) coupled with laser excitation. For [RB â 2H]2â, photodetachment action spectroscopy reveals a clear band in the visible (450â580ânm) with vibronic structure. Electron affinity and repulsive Coulomb barrier (RCB) properties of the dianion are characterized using frequency-resolved photoelectron spectroscopy, revealing a decreased RCB compared with that of fluorescein dianions due to electron delocalization over halogen atoms. Monoanions [RB â H]â and [RB â 2H]âąâ differ in nominal mass by 1âDa but are difficult to study individually using action spectroscopies that isolate target ions using low-resolution mass spectrometry. This work shows that the two monoanions are readily distinguished and probed using the IMS-photo-IMS and photo-IMS-photo-IMS strategies, providing distinct but overlapping photodissociation action spectra in the visible spectral range. Gas-phase fluorescence was not detected from photoexcited [RB â 2H]2â due to rapid electron ejection. However, both [RB â H]â and [RB â 2H]âąâ show a weak fluorescence signal. The [RB â H]â action spectra show a large Stokes shift of âŒ1700âcmâ1, while the [RB â 2H]âąâ action spectra show no appreciable Stokes shift. This difference is explained by considering geometries of the ground and fluorescing states
Action spectroscopy of the isolated red Kaede fluorescent protein chromophore
Incorporation of fluorescent proteins into biochemical systems has revolutionized the field of bioimaging. In a bottom-up approach, understanding the photophysics of fluorescent proteins requires detailed investigations of the light-absorbing chromophore, which can be achieved by studying the chromophore in isolation. This paper reports a photodissociation action spectroscopy study on the deprotonated anion of the red Kaede fluorescent protein chromophore, demonstrating that at least three isomersâassigned to deprotomersâare generated in the gas phase. Deprotomer-selected action spectra are recorded over the S1 â S0 band using an instrument with differential mobility spectrometry coupled with photodissociation spectroscopy. The spectrum for the principal phenoxide deprotomer spans the 480â660 nm range with a maximum response at â610 nm. The imidazolate deprotomer has a blue-shifted action spectrum with a maximum response at â545 nm. The action spectra are consistent with excited state coupled-cluster calculations of excitation wavelengths for the deprotomers. A third gas-phase species with a distinct action spectrum is tentatively assigned to an imidazole tautomer of the principal phenoxide deprotomer. This study highlights the need for isomer-selective methods when studying the photophysics of biochromophores possessing several deprotonation sites
Acetate Kinase Isozymes Confer Robustness in Acetate Metabolism
Acetate kinase (ACK) (EC no: 2.7.2.1) interconverts acetyl-phosphate and acetate to either catabolize or synthesize acetyl-CoA dependent on the metabolic requirement. Among all ACK entries available in UniProt, we found that around 45% are multiple ACKs in some organisms including more than 300 species but surprisingly, little work has been done to clarify whether this has any significance. In an attempt to gain further insight we have studied the two ACKs (AckA1, AckA2) encoded by two neighboring genes conserved in Lactococcus lactis (L. lactis) by analyzing protein sequences, characterizing transcription structure, determining enzyme characteristics and effect on growth physiology. The results show that the two ACKs are most likely individually transcribed. AckA1 has a much higher turnover number and AckA2 has a much higher affinity for acetate in vitro. Consistently, growth experiments of mutant strains reveal that AckA1 has a higher capacity for acetate production which allows faster growth in an environment with high acetate concentration. Meanwhile, AckA2 is important for fast acetate-dependent growth at low concentration of acetate. The results demonstrate that the two ACKs have complementary physiological roles in L. lactis to maintain a robust acetate metabolism for fast growth at different extracellular acetate concentrations. The existence of ACK isozymes may reflect a common evolutionary strategy in bacteria in an environment with varying concentrations of acetate
The regeneration capacity of the flatworm Macrostomum lignanoâon repeated regeneration, rejuvenation, and the minimal size needed for regeneration
The lionâs share of studies on regeneration in Plathelminthes (flatworms) has been so far carried out on a derived taxon of rhabditophorans, the freshwater planarians (Tricladida), and has shown this groupâs outstanding regeneration capabilities in detail. Sharing a likely totipotent stem cell system, many other flatworm taxa are capable of regeneration as well. In this paper, we present the regeneration capacity of Macrostomum lignano, a representative of the Macrostomorpha, the basal-most taxon of rhabditophoran flatworms and one of the most basal extant bilaterian protostomes. Amputated or incised transversally, obliquely, and longitudinally at various cutting levels, M. lignano is able to regenerate the anterior-most body part (the rostrum) and any part posterior of the pharynx, but cannot regenerate a head. Repeated regeneration was observed for 29 successive amputations over a period of almost 12Â months. Besides adults, also first-day hatchlings and older juveniles were shown to regenerate after transversal cutting. The minimum number of cells required for regeneration in adults (with a total of 25,000 cells) is 4,000, including 160 neoblasts. In hatchlings only 1,500 cells, including 50 neoblasts, are needed for regeneration. The life span of untreated M. lignano was determined to be about 10Â months
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