237 research outputs found
Description of Goussia kuehae n. sp. (Apicomplexa: Eimeriidae) infecting the Asian seabass, Lates calcarifer (Bloch)(Perciformes: Latidae), cultured in Malaysian fish farms
Culturing fishes in marine cages is a rapidly
developing area of marine aquaculture. The Asian
seabass Lates calcarifer (Bloch) is a fast growing good
quality fish that is readily cultured in intensive systems
in the South Asian region and in Malaysia in
particular. Although several papers have been published
to date on viral, bacterial, parasitic and fungal
organisms causing diseases in the Asian seabass, the
occurrence of a coccidian infection in this species has
only recently been recorded. We collected sporulated
and unsporulated oo¨cysts of a new species of Goussia
Labbe´, 1986, from the mucus covering the epithelium
of the intestine of L. calcarifer. This paper provides a
description of Goussia kuehae n. sp. Sporulated
oo¨cysts of this species are ellipsoidal, 37–40 lm in
length and 28–30 lm in width. The ellipsoidal sporocysts
are relatively small, 15.2–17 9 5.7–8 lm, and
located loosely in the oo¨cyst. There are residual bodies
both in the oo¨cysts and the sporocysts. Goussia kuehae
n. sp. differs from all known species of Goussia in the large size of the oo¨cysts and in having two types of
oo¨cyst residuum
Fe–FeO and Fe–Fe<sub>3</sub>C melting relations at Earth's core–mantle boundary conditions: Implications for a volatile-rich or oxygen-rich core
International audienceEutectic melting temperatures in the Fe–FeO and Fe–Fe3C systems have been determined up to 150 GPa. Melting criteria include observation of a diffuse scattering signal by in situ X-Ray diffraction, and textural characterisation of recovered samples. In addition, compositions of eutectic liquids have been established by combining in situ Rietveld analyses with ex situ chemical analyses. Gathering these new results together with previous reports on Fe–S and Fe–Si systems allow us to discuss the specific effect of each light element (Si, S, O, C) on the melting properties of the outer core. Crystallization temperatures of Si-rich core compositional models are too high to be compatible with the absence of extensive mantle melting at the core–mantle boundary (CMB) and significant amounts of volatile elements such as S and/or C (>5 at%, corresponding to >2 wt%), or a large amount of O (>15 at% corresponding to ∼5 wt%) are required to reduce the crystallisation temperature of the core material below that of a peridotitic lower mantle
Structure and density of Fe-C liquid alloys under high pressure
International audienceThe density and structure of liquid Fe-C alloys have been measured up to 58 GPa and 3,200 K by in situ X-ray diffraction using a Paris-Edinburgh press and laser-heated diamond anvil cell. Study of the pressure evolution of the local structure inferred by X-ray diffraction measurements is important to understand the compression mechanism of the liquid. Obtained data show that the degree of compression is greater for the first coordination sphere than the second and third coordination spheres. The extrapolation of the measured density suggests that carbon cannot be the only light element alloyed to iron in the Earth's core, as 8-16 at % C (1.8-3.7 wt % C) would be necessary to explain the density deficit of the outer core relative to pure Fe. This concentration is too high to account for outer core velocity. The presence of other light elements (e.g., O, Si, S, and H) is thus required
Copy Number Variants in Patients with Severe Oligozoospermia and Sertoli-Cell-Only Syndrome
A genetic origin is estimated in 30% of infertile men with the common phenotypes of oligo- or azoospermia, but the pathogenesis of spermatogenic failure remains frequently obscure. To determine the involvement of Copy Number Variants (CNVs) in the origin of male infertility, patients with idiopathic severe oligozoospermia (N = 89), Sertoli-cell-only syndrome (SCOS, N = 37)) and controls with normozoospermia (N = 100) were analysed by array-CGH using the 244A/400K array sets (Agilent Technologies). The mean number of CNVs and the amount of DNA gain/loss were comparable between all groups. Ten recurring CNVs were only found in patients with severe oligozoospermia, three only in SCOS and one CNV in both groups with spermatogenic failure but not in normozoospermic men. Sex-chromosomal, mostly private CNVs were significantly overrepresented in patients with SCOS. CNVs found several times in all groups were analysed in a case-control design and four additional candidate genes and two regions without known genes were associated with SCOS (P<1×10−3). In conclusion, by applying array-CGH to study male infertility for the first time, we provide a number of candidate genes possibly causing or being risk factors for the men's spermatogenic failure. The recurring, patient-specific and private, sex-chromosomal CNVs as well as those associated with SCOS are candidates for further, larger case-control and re-sequencing studies
X Chromosome Inactivation and Differentiation Occur Readily in ES Cells Doubly-Deficient for MacroH2A1 and MacroH2A2
Macrohistones (mH2As) are unusual histone variants found exclusively in vertebrate chromatin. In mice, the H2afy gene encodes two splice variants, mH2A1.1 and mH2A1.2 and a second gene, H2afy2, encodes an additional mH2A2 protein. Both mH2A isoforms have been found enriched on the inactive X chromosome (Xi) in differentiated mammalian female cells, and are incorporated into the chromatin of developmentally-regulated genes. To investigate the functional significance of mH2A isoforms for X chromosome inactivation (XCI), we produced male and female embryonic stem cell (ESC) lines with stably-integrated shRNA constructs that simultaneously target both mH2A1 and mH2A2. Surprisingly, we find that female ESCs deficient for both mH2A1 and mH2A2 readily execute and maintain XCI upon differentiation. Furthermore, male and female mH2A-deficient ESCs proliferate normally under pluripotency culture conditions, and respond to several standard differentiation procedures efficiently. Our results show that XCI can readily proceed with substantially reduced total mH2A content
The impact of ENSO on Southern African rainfall in CMIP5 ocean atmosphere coupled climate models
We study the ability of 24 ocean atmosphere global coupled models from the Coupled Model Intercomparison Project 5 (CMIP5) to reproduce the teleconnections between El Niño Southern Oscillation (ENSO) and Southern African rainfall in austral summer using historical forced simulations, with a focus on the atmospheric dynamic associated with El Niño. Overestimations of summer rainfall occur over Southern Africa in all CMIP5 models. Abnormal westward extensions of ENSO patterns are a common feature of all CMIP5 models, while the warming of the Indian Ocean that happens during El Niño is not correctly reproduced. This could impact the teleconnection between ENSO and Southern African rainfall which is represented with mixed success in CMIP5 models. Large-scale anomalies of suppressed deep-convection over the tropical maritime continent and enhanced convection from the central to eastern Pacific are correctly simulated. However, regional biases occur above Africa and the Indian Ocean, particularly in the position of the deep convection anomalies associated with El Niño, which can lead to the wrong sign in rainfall anomalies in the northwest part of South Africa. From the near-surface to mid-troposphere, CMIP5 models underestimate the observed anomalous pattern of pressure occurring over Southern Africa that leads to dry conditions during El Niño years
Optical and spectroscopic properties of erbium-activated modified silica glass with 1.54 μm high quantum efficiency
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