O papel do EEG Ambulatório de 24 Horas na investigação de doentes com suspeita ou diagnóstico de epilepsia : casuística da Unidade Local de Saúde de Matosinhos

O electroencefalograma (EEG) é uma ferramenta de valor diagnóstico comprovado na investigação de pacientes com eventos clínicos suspeitos de crises epilépticas. Atendendo à neurofisiologia do sinal bioeléctrico e à curta duração dos registos EEG de rotina, a sensibilidade é baixa para eventos pouco frequentes como é o caso em muitas epilepsias, mesmo após o seu acréscimo através da repetição de exames, privação de sono e proximidade temporal ao evento ictal, falhando a detecção de anomalias electroencefalográficas relevantes para o diagnóstico numa proporção importante de doentes. Neste contexto, os avanços técnicos dos últimos anos, incluindo o EEG Ambulatório 24 Horas (EEGa), vieram melhorar significativamente a capacidade para registos prolongados a custos controlados, o que resultou em ganhos importantes na utilidade clínica

Federação académica do desporto universitário

O presente relatório expõe o trabalho desenvolvido durante o período de estágio curricular no âmbito do Mestrado em Gestão do Desporto, realizado na Federação Académica do Desporto Universitário (FADU). O objetivo deste relatório consiste na caracterização do trabalho desenvolvido por um Gestor do Desporto, durante três meses, no Departamento Desportivo de uma das Federações Multidesportivas mais ativas da Europa. Para além da caracterização da Federação, pretende-se ainda transmitir o conceito e a importância do Desporto no Ensino Superior em Portugal. Demonstrar como se estrutura e organiza os Campeonatos Nacionais Universitários, entre os quais o principal evento nacional organizado pela FADU, as Fases Finais dos Campeonatos Nacionais Universitários em modalidades coletivas. Serão apresentados os objetivos e tarefas desenvolvidas ao longo do estágio, desde os trabalhos de pesquisa aos contributos para o futuro da Federação, às funções desempenhadas antes e durante as provas e as diferentes perspetivas, enquanto atleta e Staff, no decorrer das Fases Finais dos Campeonatos Nacionais Universitários Aveiro 2018.This report presents the work developed during a curricular internship of the Master's degree in Management of Sport, held in the Academic Federation of University Sports. The objective of this report is to characterize the work carried out by a Sports Manager for three months in the Sports Department of one of the most active Multi-Sport Federations in Europe. In addition to the characterization of the Federation, it is also intended to convey the concept and importance of Sports in Higher Education in Portugal. It shows how the National Academic Championships are structured and organized, including the main national event organized by FADU, the Final Phases of the National Academic Championships in collective sports. It will present the objectives and tasks developed throughout the internship, from the research to the contributions to the future of the Federation, to the functions performed before and during the tests and the different perspectives, as athlete and Staff, in the course of the Final Phases of the Championships National University Aveiro 2018

Clinical usefulness of the electroencephalogram in acute stroke: a preliminary study

Introduction: Stroke is the main cause of disability worldwide, being the first cause of death in Portugal. In the first hours of the event, the cranioencephalic CT scan (CT Scan) does not show the lesion in about 74% of cases, making validation of alternative diagnostic approaches of utmost importance. The electroencephalogram (EEG) may provide useful information for the diagnosis and prognosis of stroke. Objective: To study the potential usefulness of the EEG for the early diagnosis of acute stroke in patients with initial negative CT Scan, and for the evaluation of the functional status and risk of epilepsy. Methods: Retrospective analysis of patients with ischemic stroke who underwent EEG and acute phase CT scan between January 2014 and February 2018. Patient characteristics and stroke were classified according to the Oxfordshire Community Stroke Project (OCSP) criteria. The patients were functionally evaluated at 12 months post-stroke by the modified Rankin Scale (mRS) and the existence of post-stroke epilepsy was determined by telephone interview on February 2018. Results: Thirty patients (25 females and 5 males, mean age 70.5 years) were included. According to the OCSP were identified: 40% TACS, 37% PACS, 10% LACS and 13% POCS. 50% with acute vascular injury visible on the initial CT Scan performed with 7 hours of evolution in median. All patients underwent EEG with a median of 3 days of evolution, and slow focal activity was observed in all patients, and focal paroxysmal activity (PA) was seen in 17% of the participants. 17 patients (58% of patients) developed post-stroke epilepsy (PSE) with 4 of these having PA evidence in the initial EEG (24%). One of the patients with PA in the initial EEG did not develop epilepsy during a 4 years follow-up period. In patients without PA, the average of mRs at follow-up was 3.8 and the mortality was 24%, whereas in patients with PA, the mean of the mRs was 5.0 and the mortality was 40%. Conclusion: In this study, unlike CT Scan, the acute-phase EEG presented with abnormal features in all patients with acute stroke, therefore the EEG may potentially provide significant diagnostic information, estimates of risk for developing future epilepsy and also overall risk stratification. Further studies are needed to validate this hypothesis.info:eu-repo/semantics/publishedVersio

A Retrospective Study in Lisbon

Publisher Copyright: © 2024 by the authors.The resurgence of Mpox, predominantly among men who have sex with men (MSM), has prompted an analysis of its clinical manifestations and epidemiological patterns, particularly in individuals living with human immunodeficiency virus (HIV). This retrospective study aims to delineate and compare the clinical presentations and epidemiological characteristics of Mpox among HIV-positive and HIV-negative individuals. A total of 58 confirmed Mpox cases from a dermatology department in Lisbon were analyzed, focusing on mucocutaneous manifestations, systemic symptoms, and laboratory parameters. Our findings indicate no significant difference in disease severity and presentation between HIV-positive and HIV-negative groups, suggesting that HIV status may not be a determinant of Mpox severity, but rather an indicator of increased sexual risk behaviors, a recognized risk factor for Mpox transmission.publishersversionpublishe

Work cell for assembling small components in PCB

Flexibility and speed in the development of new industrial machines are essential factors for the success of capital goods industries. When assembling a printed circuit board (PCB), since all the components are surface mounted devices (SMD), the whole process is automatic. However, in many PCBs, it is necessary to place components that are not SMDs, called pin through hole components (PTH), having to be inserted manually, which leads to delays in the production line. This work proposes and validates a prototype work cell based on a collaborative robot and vision systems whose objective is to insert these components in a completely autonomous or semi-autonomous way. Different tests were made to validate this work cell, showing the correct implementation and the possibility of replacing the human worker on this PCB assembly task.European Regional Development Fund (ERDF) through the Operational Competitiveness and Internationalization Programme (COMPETE 2020) of the Portugal 2020 Program [Project No. 45070, " FlexASComp”; Funding Reference: POCI-01-0247FEDER-045070]

Impact of 24H Ambulatory EEG in the clinical approach to patients with suspected Epilepsy

Introdução: O eletroencefalograma constitui a técnica gold-standard para avaliar a atividade cortical epileptógenea. No entanto, a sua sensibilidade revela-se baixa nas modalidades de registo de curta duração, sendo que, neste contexto, a utilização do EEG Ambulatório de 24 horas (EEGa) veio permitir uma maior capacidade diagnóstica, enquanto registo prolongado, a custos controlados. Objetivo: Analisar o impacto do EEGa na avaliação de doentes com suspeita de epilepsia, através da sua sensibilidade e especificidade para esse diagnóstico clínico. Secundariamente, a) avaliar a sensibilidade em função da distância temporal à última crise, b) averiguar se existe relação entre a presença de atividade paroxística e lesão, c) avaliar o follow-up dos doentes e d) determinar possíveis fatores preditivos. Material e Métodos: Estudo observacional retrospetivo de uma amostra contínua dos pacientes com suspeita de epilepsia que realizaram EEGa entre maio de 2011 e maio de 2018 no Laboratório de Neurofisiologia da Unidade Local de Saúde de Matosinhos. Resultados: Amostra de 83 indivíduos, com idade média de 44.5 anos (79 adultos e 4 pediátricos). A sensibilidade foi de 97% e especificidade de 73% para o diagnóstico de epilepsia, com uma taxa de falsos positivos e falsos negativos de 5% e 7%, respetivamente. Conclusões: O EEGa deve ser um estudo neurofisiológico a considerar mais frequentemente na prática clínica, almejando um diagnóstico mais precoce de epilepsia ou contrariando este, designadamente nos casos em que os exames de primeira linha sejam normais e a convicção clínica permaneça. A brevidade obtida para o diagnóstico evita as expectáveis e demais consequências.info:eu-repo/semantics/publishedVersio

First records of Chthonerpeton arii Cascon and Lima-Verde, 1994 (Amphibia: Gymnophiona: Typhlonectidae) out of the type locality

Chthonerpeton arii was described from a large series of specimens from Limoeiro do Norte municipality, state of Ceará, Brazil. Here we provide the first records of the species out of the type locality, in the state of Bahia, and in its border with the state of Pernambuco, Brazil. We also provide color photographs of a preserved specimen

Male fertility in Arabidopsis requires active DNA demethylation of genes that control pollen tube function.

Active DNA demethylation is required for sexual reproduction in plants but the molecular determinants underlying this epigenetic control are not known. Here, we show in Arabidopsis thaliana that the DNA glycosylases DEMETER (DME) and REPRESSOR OF SILENCING 1 (ROS1) act semi-redundantly in the vegetative cell of pollen to demethylate DNA and ensure proper pollen tube progression. Moreover, we identify six pollen-specific genes with increased DNA methylation as well as reduced expression in dme and dme;ros1. We further show that for four of these genes, reinstalling their expression individually in mutant pollen is sufficient to improve male fertility. Our findings demonstrate an essential role of active DNA demethylation in regulating genes involved in pollen function

Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal

Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.info:eu-repo/semantics/publishedVersio