Using morphometrics to reappraise old collections: The study case of the Congo Basin Middle Stone Age bifacial industry

Despite its strategic location within the continent, Central Africa is rarely integrated into the reconstruction of population dynamics during the Middle Stone Age (MSA) of Africa, especially in terms of the emergence, diffusion and behavioural patterns of Homo sapiens. However, hundreds of sites have been discovered in Central Africa during the 20th century and attributed to the Lupemban, one of the main MSA technological complexes of the region. This complex is mainly characterised by typological criteria based on the numerous bifacial pieces found in the Congo Basin and interpreted as an adaption to the rainforest environment. Most of these Lupemban assemblages have not been studied for decades and thus it is particularly difficult to assess their diversity. This paper presents a detailed combined morphometrical approach (linear measurements and indices, Log Shape Ratio, Elliptic Fourier Analysis) to take a fresh and rigorous look at the Lupemban bifacial tools. We discuss the comparison of different morphometrical approaches to deal with “old” collections for which contexts, particularly chronological ones, are partially missing. We present the results of this approach on three assemblages of bifacial pieces gathered in the 1930s and late 1960s. We quantify their variability and discuss not only their homogeneity but also the variation of a Lupemban hallmark, namely the “Lupemban point”

Peptidyl-prolyl cis-trans isomerases (immunophilins) and their roles in parasite biochemistry, host-parasite interaction and antiparasitic drug action.

Immunophilin is the collective name given to the cyclophilin and FK506-binding protein (FKBP) families. As the name suggests, these include the major binding proteins of certain immunosuppressive drugs: cyclophilins for the cyclic peptide cyclosporin A and FKBPs for the macrolactones FK506 and rapamycin. Both families, although dissimilar in sequence, possess peptidyl-prolyl <i>cis-trans</i> isomerase activity in vitro and can play roles in protein folding and transport, RNA splicing and the regulation of multiprotein complexes in cells. In addition to enzymic activity, many immunophilins act as molecular chaperones. This property may be conferred by the isomerase domain and/or by additional domains. Recent years have seen a great increase in the number of known immunophilin genes in parasitic protozoa and helminths and in many cases their products have been characterized biochemically and their temporal and spatial expression patterns have been examined. Some of these genes represent novel types: one example is a <i>Toxoplasma gondii</i> gene encoding a protein with both cyclophilin and FKBP domains. Likely roles in protein folding and oligomerisation, RNA splicing and sexual differentiation have been suggested for parasite immunophilins. In addition, unexpected roles in parasite virulence (Mip FKBP of <i>Trypanosoma cruzi</i>) and host immuno-modulation (e.g. 18-kDa cyclophilin of <i>Toxoplasma gondii</i>) have been established. Furthermore, in view of the potent antiparasitic activities of cyclosporins, macrolactones and nonimmunosuppressive derivatives of these compounds, immunophilins may mediate drug action and/or may themselves represent potential drug targets. Investigation of the mechanisms of action of these agents may lead to the design of potent and selective antimalarial and other antiparasitic drugs. This review discusses the properties of immunophilins in parasites and the 'animal model' <i>Caenorhabditis elegans</i> and relates these to our understanding of the roles of these proteins in cellular biochemistry, host-parasite interaction and the antiparasitic mechanisms of the drugs that bind to them

An update on the mosquito fauna and mosquito-borne diseases distribution in Cameroon

The expansion of mosquito-borne diseases such as dengue, yellow fever, and chikungunya in the past 15 years has ignited the need for active surveillance of common and neglected mosquito-borne infectious diseases. The surveillance should be designed to detect diseases and to provide relevant field-based data for developing and implementing effective control measures to prevent outbreaks before significant public health consequences can occur. Mosquitoes are important vectors of human and animal pathogens, and knowledge on their biodiversity and distribution in the Afrotropical region is needed for the development of evidence-based vector control strategies. Following a comprehensive literature search, an inventory of the diversity and distribution of mosquitoes as well as the different mosquito-borne diseases found in Cameroon was made. A total of 290 publications/reports and the mosquito catalogue website were consulted for the review. To date, about 307 species, four subspecies and one putative new species of Culicidae, comprising 60 species and one putative new species of Anopheles, 67 species and two subspecies of Culex, 77 species and one subspecies of Aedes, 31 species and one subspecies of Eretmapodites, two Mansonia, eight Coquillettidia, and 62 species with unknown medical and veterinary importance (Toxorhynchites, Uranotaenia, Mimomyia, Malaya, Hodgesia, Ficalbia, Orthopodomyia, Aedeomyia, and Culiseta and Lutzia) have been collected in Cameroon. Multiple mosquito species implicated in the transmission of pathogens within Anopheles, Culex, Aedes, Eretmapodites, Mansonia, and Coquillettidia have been reported in Cameroon. Furthermore, the presence of 26 human and zoonotic arboviral diseases, one helminthic disease, and two protozoal diseases has been reported. Information on the bionomics, taxonomy, and distribution of mosquito species will be useful for the development of integrated vector management programmes for the surveillance and elimination of mosquito-borne diseases in Cameroon

The Waddlia Genome: A Window into Chlamydial Biology

Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is a potential agent of miscarriage in humans and abortion in ruminants. Due to the lack of genetic tools to manipulate chlamydia, genomic analysis is proving to be the most incisive tool in stimulating investigations into the biology of these obligate intracellular bacteria. 454/Roche and Solexa/Illumina technologies were thus used to sequence and assemble de novo the full genome of the first representative of the Waddliaceae family, W. chondrophila. The bacteria possesses a 2′116′312bp chromosome and a 15′593 bp low-copy number plasmid that might integrate into the bacterial chromosome. The Waddlia genome displays numerous repeated sequences indicating different genome dynamics from classical chlamydia which almost completely lack repetitive elements. Moreover, W. chondrophila exhibits many virulence factors also present in classical chlamydia, including a functional type III secretion system, but also a large complement of specific factors for resistance to host or environmental stresses. Large families of outer membrane proteins were identified indicating that these highly immunogenic proteins are not Chlamydiaceae specific and might have been present in their last common ancestor. Enhanced metabolic capability for the synthesis of nucleotides, amino acids, lipids and other co-factors suggests that the common ancestor of the modern Chlamydiales may have been less dependent on their eukaryotic host. The fine-detailed analysis of biosynthetic pathways brings us closer to possibly developing a synthetic medium to grow W. chondrophila, a critical step in the development of genetic tools. As a whole, the availability of the W. chondrophila genome opens new possibilities in Chlamydiales research, providing new insights into the evolution of members of the order Chlamydiales and the biology of the Waddliaceae

Formes et fonctions au sein des industries lithiques de la fin du Pléistocène et du début de l'Holocène en Asie du Sud-Est (un nouvel apport à la compréhension des comportements humains)

Les progrès des travaux sur la préhistoire de l Asie du Sud-Est dépendent dans une large mesure de l étude des industries lithiques. Ces dernières sont bien différentes de celles connues en Europe et délicates à caractériser par la typo-technologie. L exemple de la grotte de Song Terus (Indonésie) est parfait pour l étude d un (ou plusieurs) des groupes humains qui se sont dispersés sur les archipels après le dernier maximum glaciaire. L industrie lithique de ce site provenant des niveaux du début de l Holocène, entre 11 000 et 5 000 ans BP, est essentiellement constituée d éclats en silex, souvent corticaux. Ce travail, en menant une étude morphologique, morphométrique et tracéologique montre que l intérêt des Hommes ne se tournait pas vers des pièces de formes spécifiques mais vers la présence de certaines zones actives de morphologies particulières, associées à des zones de préhension permettant une meilleure utilisation. L importance du travail du végétal a aussi été relevée.The research progress about the prehistory of South-East Asia depends largely on the study of the lithic industries. The latter are different from those known in Europe and delicate to characterize by means of the typo-technology which seems simple and monotonous through time. The example of Song Terus (Indonesia) is perfect to study one (or several) human groups which scattered onto the archipelagoes after the Last Glacial Maximum. The lithic industry of this site, coming from layers which correspond to the beginning of the Holocene, from 11 000 to 5 000 years ago, mainly consists of flint flakes, often cortical. This work carries out morphological, morphometrical and usewear analysis studies and show that human interest was not directed towards artifacts of specific forms but towards the presence of certain active areas of particular morphology, associated to prehensile zones permitting a better utilization. The importance of vegetal processing has also been noticed.PARIS-Museum Hist.Naturelle (751052304) / SudocSudocFranceF

Stone Tool Forms and Functions: A Morphometric Analysis of Modern Humans' Stone Tools From Song Terus Cave (Java, Indonesia)

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Sequence Analysis of Grip and Manipulation During Tool Using Tasks: a New Method to Analyze Hand Use Strategies and Examine Human Specificities

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Conditioning of the raw materials on discoid exploitation strategies during the Early Middle Palaeolithic: the example of Payre level D (South-East France)

International audienceIn the last decades, a growing attention was paid towards the exploitation of other lithic raw materials than flint. In this context, the lithic assemblage of Payre level D, dated to the end of the MIS 6, offers the opportunity to make some considerations about the modalities of a discoid technology on various lithic raw materials. Payre is a very well-known Middle Palaeolithic site located on the right edge of the Middle Rhône valley. Three main archaeological levels attest an intense human frequentation of the site between the end of MIS 8 and the beginning of MIS 5. The rich faunal and lithic assemblages, recovered during systematic excavations from 1990 to 2002, have been deeply analysed for a precise understanding of the site formation processes and the modalities of the human occupation of the area. The exploitation of different local and semi-local raw materials made Payre an interesting case to observe how lithic resources with different petrographic characteristics were exploited by the Neanderthal groups. The present work aims to observe how the lithic raw materials have affected the discoid reduction sequences. This knapping method is widely attested in archaeological level D, the last large phase of human occupation at Payre. Among the different raw materials exploited (flint, vein quartz, quartzite, basalt and limestone), the discoid method is just applied on flint and vein quartz. The new technological analysis carried out focuses for the first time on the characteristics of the discoid technology present in level D and on the differences in discoid reduction sequences depending on the raw materials. The results indicate some expedients used to apply discoid reduction strategies on vein quartz and adapt this technology to the features of a coarse-grained stone. The use of such expedients testifies a large capability to manage the raw materials but also of the discoid method itself. Results allow to make considerations on some of the criteria proper to the technological definition of the method

Neanderthal's microlithic tool production and use, the case of Tata (Hungary)

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