Lit de la Seine

Le Groupement de recherches archéologiques subaquatiques a poursuivi ses prospections en vue d’identifier de nouveaux sites sur le cours de la Haute-Seine. À Étiolles (91), une prospection menée en amont des lies-aux-Paveurs, a révélé une digue empierrée submergée qui barre partiellement le petit bras de la Seine (xixe s.). À la pointe de l’île, une ligne de quelques pieux d’une dizaine de centimètres de diamètre, a été localisée. Ils sont enfoncés dans une couche de falaize et le bois qui le..

Histoire de l’habitat

Augustin Berque, directeur d’études avec Philippe Bonnin et Cynthia Ghorra-Gobin, directeur de recherche au CNRS Cf. le compte rendu à la rubrique « Géographie »

Anthropologie de l’habiter

Augustin Berque, directeur d’étudesPhilippe Bonnin, directeur d’études au CNRSAlessia de Biase, maître-assistant à l’ENSAPB Poétique de l’habiter Les premières séances du séminaire (ensuite interrompu pour raisons de santé) ont été consacrées au thème de la cosmicité chinoise traditionnelle. Le séminaire collectif s’est cependant poursuivi jusqu’à la fin de l’année en l’absence du directeur d’études, avec notamment deux conférences de Tsuchiya sur le thème ci-dessus mentionné Publications La ..

Méthode d'optimisation temporelle des algorithmes de vision

- Compte tenu de la puissance de calcul disponible sur les robots mobiles, la recherche d'une optimisation temporelle devient un élément fondamental. Dans ce papier, une méthode est proposée, illustrée par deux applications réalisées. Enfin, nous présentons des résultats numériques, issus de « benchmarks » quantifiant les améliorations réalisées

Whole genome mapping of 5' RNA ends in bacteria by tagged sequencing : A comprehensive view in Enterococcus faecalis

Enterococcus faecalis is the third cause of nosocomial infections. To obtain the first comprehensive view of transcriptional organizations in this bacterium, we used a modified RNA-seq approach enabling to discriminate primary from processed 5'RNA ends. We also validated our approach by confirming known features in Escherichia coli. We mapped 559 transcription start sites and 352 processing sites in E. faecalis. A blind motif search retrieved canonical features of SigA- and SigN-dependent promoters preceding TSSs mapped. We discovered 95 novel putative regulatory RNAs, small- and antisense RNAs, and 72 transcriptional antisense organisations. Presented data constitute a significant insight into bacterial RNA landscapes and a step towards the inference of regulatory processes at transcriptional and post-transcriptional levels in a comprehensive manner

Transcriptional Landscape of a blaKPC-2 Plasmid and Response to Imipenem Exposure in Escherichia coli TOP10

The diffusion of KPC-2 carbapenemase is closely related to the spread of Klebsiella pneumoniae of the clonal-group 258 and linked to IncFIIK plasmids. Little is known about the biology of multi-drug resistant plasmids and the reasons of their successful dissemination. Using E. coli TOP10 strain harboring a multi-replicon IncFIIK-IncFIB blaKPC−2-gene carrying plasmid pBIC1a from K. pneumoniae ST-258 clinical isolate BIC-1, we aimed to identify basal gene expression and the effects of imipenem exposure using whole transcriptome approach by RNA sequencing (RNA-Seq). Independently of the antibiotic pressure, most of the plasmid-backbone genes were expressed at low levels. The most expressed pBIC1a genes were involved in antibiotic resistance (blaKPC−2, blaTEM and aph(3′)-I), in plasmid replication and conjugation, or associated to mobile elements. After antibiotic exposure, 34% of E. coli (pBIC1a) genome was differentially expressed. Induction of oxidative stress response was evidenced, with numerous upregulated genes of the SoxRS/OxyR oxydative stress regulons, the Fur regulon (for iron uptake machinery), and IscR regulon (for iron sulfur cluster synthesis). Nine genes carried by pBIC1a were up-regulated, including the murein DD-endopeptidase mepM and the copper resistance operon. Despite the presence of a carbapenemase, we observed a major impact on E. coli (pBIC1a) whole transcriptome after imipenem exposure, but no effect on the level of transcription of antimicrobial resistance genes. We describe adaptive responses of E. coli to imipenem-induced stress, and identified plasmid-encoded genes that could be involved in resistance to stressful environments

Anthropologie de l’habiter

Augustin Berque, directeur d’étudesPhilippe Bonnin, directeur de recherche au CNRSAlessia de Biase, maître-assistant à l’ENSAPB La poétique de l’habiter Cf. le compte rendu du séminaire « Questions de mésologie », à la rubrique Géographie Augustin Berque, directeur d’étudesPhilippe Bonnin, directeur de recherche au CNRSMarc Bourdier, professeur à l’ENSAPLVNicolas Fiévé, directeur d’études à l’EPHEMurielle Hladik, maître-assistante à l’ENSA de Saint-ÉtienneCorinne Tiry, architecte DPLG, ingéni..

Deletion of the myeloid endothelin-B receptor confers long-term protection from angiotensin II-mediated renal, retinal & vascular injury

International audienceThe endothelin system may be an important player in hypertensive end-organ injury as endothelin-1 increases blood pressure and is pro-inflammatory. The immune system is emerging as an important regulator of blood pressure and we have shown that the early hypertensive response to angiotensin-II infusion was amplified in mice deficient of myeloid endothelin-B (ETB) receptors (LysM-CreEdnrblox/lox). Hypothesizing that these mice would display enhanced organ injury, we gave angiotensin-II to LysM-CreEdnrblox/lox and littermate controls (Ednrblox/lox) for six weeks. Unexpectedly, LysM-CreEdnrblox/lox mice were significantly protected from organ injury, with less proteinuria, glomerulosclerosis and inflammation of the kidney compared to controls. In the eye, LysM-CreEdnrblox/lox mice had fewer retinal hemorrhages, less microglial activation and less vessel rarefaction. Cardiac remodeling and dysfunction were similar in both groups at week six but LysM-CreEdnrblox/lox mice had better endothelial function. Although blood pressure was initially higher in LysM-CreEdnrblox/lox mice, this was not sustained. A natriuretic switch at about two weeks, due to enhanced ETB signaling in the kidney, induced a hypertensive reversal. By week six, blood pressure was lower in LysM-CreEdnrblox/lox mice than in controls. At six weeks, macrophages from LysM-CreEdnrblox/lox mice were more anti-inflammatory and had greater phagocytic ability compared to the macrophages of Ednrblox/lox mice. Thus, myeloid cell ETB receptor signaling drives this injury both through amplifying hypertension and by inflammatory polarization of macrophages