Relaciones entre estilos de apego y Trastornos de la Conducta Alimentaria

Treball Final de Grau en Psicologia. Codi: PS1048. Curs acadèmic: 2016/2017Objective: It is well known, since recent research, the link between attachment styles in childhood and the development of psychopathology in the adult. In this study we expect to describe and analyze the differences in the different facets of attachment, measured by the CaMir-R questionnaire, comparing a sample of patients with a diagnosis of Eating Disorder (ED) with another sample of healthy subjects equivalent in socio-demographic variables. Material and method: We compared the scores obtained in the reduced version of the CaMir (CaMir-R) to assess the attachment in a sample of 46 subjects divided in two groups: 23 patients who come from a specialized unit in ED and 23 subjects equivalent in age, gender and level of studies from a sample of the general population. The Eating Attitudes Test (EAT) was also used to assess the type and severity of symptoms in the group of patients with ED. Results: There were significant differences between groups in the “Family concern” dimension (t = 8.89, p = .00), “Parent interference” dimension (t=6.28; p=.00), “Self-reliance and resentment against parents” dimension (t = 4.49, p = .00) and “Infant trauma” dimension (t= 2.47, p = .02). There were no significant correlations between attachment level and type of symptoms in the group of patients with ED. Conclusion: Despite some limitations, the results obtained are consistent with those of other investigations carried out and support the idea that some dimensions of insecure attachment are related to the development of an eating disorder. In addition, family concerns and parental interference proved to be clearly different between the two groups.Objetivo: Es bien sabido, desde la investigación reciente, la relación entre los estilos de apego en la infancia y el desarrollo de psicopatología en el adulto. En este trabajo pretendemos describir y analizar las diferencias en las distintas facetas del apego, medidas por el cuestionario CaMir-R, comparando una muestra de pacientes con diagnóstico de Trastorno de la Conducta Alimentaria (TCA) con otra muestra de sujetos sanos equiparados en variables socio-demográficas. Material y método: Se compararon las puntuaciones obtenidas en la versión reducida del CaMir (CaMir-R) para evaluar el apego en una muestra de 46 sujetos divididos en dos grupos: 23 pacientes que acudían a una Unidad especializada en TCA y 23 sujetos equiparados en edad, género y nivel de estudios procedentes de una muestra de la población general. También se aplicó el Eating Attitudes Test (EAT) para evaluar el tipo y gravedad de los síntomas en el grupo de pacientes con TCA. Resultados: Se observaron diferencias significativas entre ambos grupos en las dimensiones “Preocupación familiar” (t=8.89; p=.00), “Interferencia de los padres” (t=6.28; p=.00), “Autosuficiencia y rencor contra los padres” (t=4.49; p=.00) y “Traumatismo infantil” (t=2.47; p=.02). No se encontraron correlaciones significativas entre las puntuaciones de apego y la gravedad o tipo de síntomas en el grupo de pacientes con TCA. Conclusión: A pesar de algunas limitaciones, los resultados obtenidos concuerdan con los de otras investigaciones realizadas y soportan la idea de que algunas dimensiones características del apego inseguro se relacionan con el desarrollo de un trastorno de la conducta alimentaria. Además, la preocupación familiar y la interferencia de los padres resultaron ser dimensiones claramente diferenciadoras entre ambos grupos

ICT in online and offline retailing

Treball Final de Grau en Administració d'Empreses. Codi: AE1049. Curs acadèmic: 2015-201

The Leishmania infantum PUF proteins are targets of the humoral response during visceral leishmaniasis

<p>Abstract</p> <p>Background</p> <p>RNA-binding proteins of the PUF family share a conserved domain consisting of tandemly repeated 36-40 amino acid motifs (typically eight) known as Puf repeats. Proteins containing tandem repeats are often dominant targets of humoral responses during infectious diseases. Thus, we considered of interest to analyze whether <it>Leishmania </it>PUF proteins result antigenic during visceral leishmaniasis (VL).</p> <p>Findings</p> <p>Here, employing whole-genome databases, we report the composition, and structural features, of the PUF family in <it>Leishmania infantum</it>. Additionally, the 10 genes of the <it>L. infantum </it>PUF family were cloned and used to express the <it>Leishmania </it>PUFs in bacteria as recombinant proteins. Finally, the antigenicity of these PUF proteins was evaluated by determining levels of specific antibodies in sera from experimentally infected hamsters. The <it>Leishmania </it>PUFs were all recognized by the sera, even though with different degree of reactivity and/or frequency of recognition. The reactivity of hamster sera against recombinant LiPUF1 and LiPUF2 was particularly prominent, and these proteins were subsequently assayed against sera from human patients. High antibody responses against rLiPUF1 and rLiPUF2 were found in sera from VL patients, but these proteins resulted also recognized by sera from Chagas' disease patients.</p> <p>Conclusion</p> <p>Our results suggest that <it>Leishmania </it>PUFs are targets of the humoral response during <it>L. infantum </it>infection and may represent candidates for serodiagnosis and/or vaccine reagents; however, it should be kept in mind the cross-reactivity of LiPUFs with antibodies induced against other trypanosomatids such as <it>Trypanosoma cruzi</it>.</p

Per aquí passa el canal!

Projecte presentat a Racons Públics - FAD 2013Sel·leccionatPostprint (published version

La "odología" como instrumento de integración de las carreteras en el paisaje

El objetivo principal de este TFM es la utilización del término "odología" para la denominación de la suma de los distintos procesos de estudios y análisis previos en una carretera para realizar su correcta integración en el paisaje que la rodea, tanto desde el punto de vista del observador que la ve desde fuera, como desde el del viajero que observa el paisaje desde un vehículo que transita por la carretera.Bonet Viana, M. (2014). La "odología" como instrumento de integración de las carreteras en el paisaje. http://hdl.handle.net/10251/47957.Archivo delegad

Nuevas estrategias terapéuticas frente a la infección por el VIH-1

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 15-07-2014The present work is composed of two main goals: “Identification and functional analysis of the HIV-1 Nef PGPG motif, responsible for the interaction with the SH2 domain of Lck, as a new potential therapeutic target” and “Study of the in vitro and ex vivo effects of bryostatin-1 in combination with the antiretrovirals maraviroc or Atripla® on viral reactivation and the inhibition of novel infection issues”. SECTION 1: HIV-1 Nef affects pivotal processes in the infected host cell including T cell receptor signalling. Most of these effects involve the physical association with and alteration of subcellular localization of the host cell kinase Lck. Retargeting of Lck depends on a PxxP motif in Nef that binds to SH3 domains. Moreover, the SH2 domain of Lck was reported to contribute to interactions with Nef, however the identity of the HIV-1 Nef motif responsible for this interaction has remained unknown. To search for the elusive SH2 binding site we performed a functional alanine scanning analysis along a discrete but highly conserved region at the core of HIV-1 Nef. We identified a PGPG motif encompassing residues 121-137 of HIV-1NL4.3 Nef to be required for the interaction with Lck-SH2. The PGPG motif was critical for Lck dependent Nef functions, including CD4 downregulation, optimal infectivity of viruses produced in T cells as well as intracellular recruitment of Lck. Unexpectedly, we found that the PGPG motif also participated in the binding to the Lck-SH3 domain, indicative of the synergy between PxxP-SH3 and PGPG-SH2 interaction. In accordance, the PGPG motif also participated on Nef dependent inhibition of host cell actin dynamics and MHC-I downregulation, which are PxxP dependent activities of Nef that do not involve Lck. In conclusion, the PGPG motif can mediate interactions of Nef with SH2 domains and supports the activity of the Nef PxxP motif. Thus, we propose the conserved PGPG motif in HIV-1 Nef as a potential pharmacological target for interference with Nef function in HIV-1 infection. SECTION 2: Despite antiretroviral therapy successfully controls HIV-1 viremia in most AIDS patients, virus latency establishment early upon infection impedes HIV-1 eradication in HIV-1+ patients. Bryostatin-1 (BRYO) inhibits in vitro HIV-1 infection of CD4 T lymphocytes (as it downregulates CD4 and CXCR4, viral receptor and coreceptor, respectively) and, at the same time, reactivates virus from latency through PKC/NF-kB pathway activation. Prior to design clinical studies with BRYO to assess its real impact on the size of the HIV-1 latent reservoirs, the potential in vitro effect of BRYO in combination with the antiretrovirals (AR) maraviroc (MVC) and Atripla® (ATP) has been determined. Jurkat-LTRGFP-R5 cell line and two latent and reactivatable HIV-1-infected lymphocytic or monocytic clonal cell lines (J89GFP and THP89GFP respectively) were used as latency models. BRYO reactivated latent HIV-1, reaching levels up to 80% in THP89GFP cells, even in combination with MVC or ATP. Moreover, when AR pre-treated reporter TZM-bl cells were co-cultured with BRYO treated THP89GFP, new infections of reactivated HIV-189.6 were inhibited 50% or 80% for MVC or ATP pre-treated cells, respectively. Remarkably, when AR were combined with BRYO, the combinations maintained the antiviral effect of the drugs with the maximum rate of inhibition by its own. In addition, BRYO-mediated downregulation of surface CD4 and CXCR4 in PBMC was not affected when it was used along with other AR and no hiperactivation or high proliferation effects were observed in these cells. Significantly, we found that BRYO strongly stimulated the viral promoter (LTR) transcription by activating the transcription factor NF-κB in human primary astrocytes and in the astrocytoma cell line U87, suggesting that the reactivation effect of BRYO could be especially important in a cellular reservoir such as astrocytes. Moreover, BRYO was also tested ex vivo for HIV-1 induction in CD4 T cells isolated from infected individuals receiving HAART and was found to exhibit potent induction activity. This work is the first to demonstrate that AR combination with BRYO do not interfere with BRYO activity (in terms of reactivation of latently infected cells and partial inhibition of infection) neither with AR antiviral activity. Thus, we propose BRYO as a viral latency reactivator compound appropriate to be combined with actual antiretroviral treatments in order to purge the viral reservoirs

The TALP–UPC Spanish–English WMT biomedical task: bilingual embeddings and char-based neural language model rescoring in a phrase-based system

This paper describes the TALP–UPC system in the Spanish–English WMT 2016 biomedical shared task. Our system is a standard phrase-based system enhanced with vocabulary expansion using bilingual word embeddings and a characterbased neural language model with rescoring. The former focuses on resolving outof- vocabulary words, while the latter enhances the fluency of the system. The two modules progressively improve the final translation as measured by a combination of several lexical metrics.Postprint (published version