Style radzenia sobie ze stresem a satysfakcja z życia wśród menadżerów

Prezentowana praca ma charakter teoretyczny i empiryczny. Badania przeprowadzono w celu poszukiwania zależności występujących pomiędzy stylami radzenia sobie ze stresem, a satysfakcją z życia odczuwaną przez menadżerów. Badaniami objęto 100 osób, w tym 30 kobiet i 70 mężczyzn w wieku od 30 do 50 lat. W badaniu skorzystano z następujących testów psychologicznych: Kwestionariusz Radzenia Sobie w Sytuacjach Stresowych CISS N.S. Endlera i J.D.A. Parkera, Skali Satysfakcji z Życia SWLS E. Diener, R. A. Emmons, R. J. Larson, S. Griffin. oraz Kwestionariusza Ankiety własnej konstrukcji. Dokonanie analiz statystycznych oraz psychologicznych zebranego materiału empirycznego przyczyniło się do powstania następujących wniosków końcowych. Menadżerowie z niższą satysfakcją życiową częściej stosują styl skoncentrowany na emocjach oraz styl skoncentrowany na unikaniu w radzeniu sobie ze stresem, natomiast menadżerowie z wyższą satysfakcją życiową częściej stosują styl skoncentrowany na zadaniu. Natomiast w grupie badanych kobiet i mężczyzn pozycja zawodowa jest w większości przypadków istotnym determinantem poczucia satysfakcji z życia wśród mężczyzn.The presented thesis is both theoretical and empirical. The research was carried out in order to search for relationships between the styles of coping with stress, and the life satisfaction felt by managers. The study comprised of 100 managers, including 30 women and 70 men aged ranging from 30 to 50 years. The following psychological tests were used in the study: CISS N.S. Endler and J.D.A. Parker, SWLS Life Satisfaction Scale E. Diener, R. A. Emmons, R. J. Larson, S. Griffin. and a self-constructed questionnaire. Performing statistical and psychological analyzes of the collected empirical material contributed to the following final conclusions. Managers with lower life satisfaction are more likely to use an emotion-focused style and an avoidance style to deal with stress, while managers with higher life satisfaction are more likely to use a task-focused style. On the other hand, in the group of surveyed women and men, acquired the professional position in the most cases is a significant determinant of the sense of life satisfaction among the men

Does the conditioned medium trigger the adipose-derived mesenchymal stem cells differentiation into Chondrocytes?

Background: Cartilage is an avascular tissue that has limited regenerative capacity. Stem cells, especiallymesenchymal stem cells (MSCs), have been regarded as a promising cell source for cartilage repair dueto their multi-lineage differentiation potential. Induction of mesenchymal stem cells into functional chondrocytesmay resolve the above problems in the cartilage repair. However, the imperative is to developeffective strategies for chondrogenic differentiation. The aim of this study is to evaluate if the induction ofadipose-derived mesenchymal stem cells (ADSCs) differentiation toward chondrocytes is possible withthe use of conditioned medium derived from chondrocytes culture. Material and methods: Conditioned medium (CM) from Normal chondrocyte cell line CRL 2648 was used to culture ADSCs, at the same time ADSCs with standard growth medium were used as a control. During this experiment, the phenotype was assessed using flow cytometry (CD90, CD44, CD45 and CD45), and Immunohistochemicalstaining was used to determine differentiation (anti-aggrecan, anti-CD15, anti-collagen II, anti-TRA-2-49). Results: ADSCs propagated rapidly in vitro and formed a homogenous fibroblast-like morphology. 0% of testedcells showed the expression of CD90 and CD44, while more than 90% did not reveal the expression ofCD34 and CD45. Results obtained in this study showed weak cytoplasmatic expression of aggrecanand alkaline phosphatase (TRA protein) and lack of CD151 expression. However, the majority of ADSCsexpanded with CM expressed type II collagen indicating that the chondrocyte-secreted factors inducedchondrogenic commitment during expansion. Conclusions: In the natural environment of living organisms, the chondrogenic differentiation of stem cells involve multiple signalling pathways. We can, to an extent mimic these signals in vitro however, for the clinicalpurposes it is very important to develop well-defined and efficient in vitro protocols. Our results indicatethat CM may regulate and induce differentiation of ADSCs into the chondrocyte lineage and can servealso as an in vitro model for studying specific lineage commitment

Inflammation and hypoxia in atherosclerosis, coronary artery disease, and heart failure

Inflammation is a process that occurs after tissue damage or counteract infection. It is a complex reaction involving various cells, proteins (chemokines, acute-phase proteins) and other factors. The precise understanding of the mechanisms affecting the distribution of inflammatory cells and their modulators in areas of inflammation may have a crucial role in the development of strategies blocking inflammatory processes. Hypoxia leads to deprivation of oxygen and when it develops, various mechanisms come into action to alleviate any consequences that it might cause. Hypoxia inducible factor (HIF) is one of the most important factors involved in the response to hypoxia. Recent studies provide a better understanding of both, inflammation and hypoxia, in the development of atherosclerosis and various diseases in the spectrum of cardiology

Can bone marrow mesenchymal stem cells regenerate the myocardium?

Background: Cardiovascular diseases are the serious clinical problem, especially the loss of viable myocytes.A new approach, which provides a novel method for the treatment, is a tissue engineering andregenerative medicine. One of the current cell types used as a source to improve cardiac tissue repair,are MSCs. The aim of this study was to check if the 5’azacitidine and growth factors from cardiomyocytecell line initiate the differentiation of MSCs toward cardiomyocytes. Material and methods: Bone marrow MSCs were isolated and their biological features have been characterized.Conditioned media were prepared with the use of 5’azacitidine and growth factors releasedfrom cardiomyocytes. Results: The transdifferentiating process has been confirmed by the expression of specific muscle markers.Conditioned medium from cardiomyocyte cell line, as well as, 5’azacitidine induced muscle differentiationprocess in bone marrow MSCs in comparison to the control. Conclusions: Presented in this study data support the conclusion that this concept may represent a promisingstrategy for the repair of cardiac tissue, however, further experiments are necessary

Implementation of the Polish version of the 11th revision of the International Statistical Classification of Diseases and Related Health Problems (ICD-11): importance for oncology

Member States of the World Health Organization (WHO), after several years of joint review, approved and implemented the update of the International Statistical Classification of Diseases and Related Health Problems (ICD) in May 2019. Usually, the abbreviated name ICD-11 (International Classification of Diseases, 11th Revision) is used. The new version was created in fully digital form with a search easy-to-use search engine available to every user. Many changes were introduced, and the most important is the redesign of the coding system to adapt it for digital use. ICD-11 codes are divided into main and supplementary codes. Main codes are at least 4 characters long, and 2 levels of extensions, up to 7 characters, are possible. In Poland, the entire process of implementing the ICD-11 is carried out as part of a project coordinated by the Medical Center for Postgraduate Education in cooperation with the Department of Healthcare of the Ministry of Health and the e-Health Centre. The implementation of the new version and the official introduction of ICD-11 in Poland must be preceded primarily by the amendment of legal acts (laws and regulations) and orders of the President of the National Health Fund, such as those regarding the reimbursement for refunded services and the keeping of medical records. An important element is the change in the cluster codes in oncology. Selected oncology groups were based on analyses of international reports on morbidity, mortality, cancer registries, and clinical reports. Cluster 02, which deals with cancer, contains 8 subsections detailing disease states associated with abnormal or uncontrolled cell proliferation. This article summarizes and discusses the most important changes in ICD-11, along with providing an introduction to the classification rules in the coding system and individual subsections on cancer

Current management of cervical cancer in Poland : analysis of the questionnaire trial for the years 2002-2014 in relation to ASCO 2016 recommendations

OBJECTIVES:To assess the survival of patients with cervical cancer (CC). Since the recommendations concerning cervical cancer management adopted by Polish medical societies do not differ significantly from the ESGO or non-European guidelines, and the fact that evaluation of the system for CC treatment in Poland, as well as the mortality rate of Polish women with CC, which is 70% higher than the average for European Union (EU) countries, justifies the hypothesis that treatment of CC in Poland deviates from the Polish and international recommendations. This article puts forward the current management of cervical cancer in Poland and discusses it in the context of ASCO guidelines. MATERIAL AND METHODS:A survey retrospective multicenter analysis of the medical records of 1247 patients with cervical cancer who underwent treatment for disease and who had completed at least two years of follow-up. RESULTS:Although concurrent radiotherapy and chemotherapy is a standard treatment of FIGO IB to IVA cervical cancer patients in enhanced- and maximum-resources settings, in our analysis, we found that the percentage of women subjected to chemotherapy was lower than in countries where total survival rates were lower. CONCLUSION:Within the IA to II A cervical cancer patients studied group, the methods of treatment remained in line with ASCO guidelines for countries with the highest standard of care. Although concurrent radiotherapy and chemotherapy is a standard treatment of FIGO IB to IVA cervical cancer patients in enhanced- and maximum-resources settings, in our analysis, we found that the percentage of women subjected to chemotherapy was lower than in countries where total survival rates were lower. Our findings, together with the inconsistencies within the cervical cancer screening program, may be one of the explanations of poorer survival rate of women with cervical cancer in Poland

Loss of the MAF Transcription Factor in Laryngeal Squamous Cell Carcinoma

MAF is a transcription factor that may act either as a tumor suppressor or as an oncogene, depending on cell type. We have shown previously that the overexpressed miR-1290 influences MAF protein levels in LSCC (laryngeal squamous cell carcinoma) cell lines. In this study, we shed further light on the interaction between miR-1290 and MAF, as well as on cellular MAF protein localization in LSCC. We confirmed the direct interaction between miR-1290 and MAF 3'UTR by a dual-luciferase reporter assay. In addition, we used immunohistochemistry staining to analyze MAF protein distribution and observed loss of MAF nuclear expression in 58% LSCC samples, of which 10% showed complete absence of MAF, compared to nuclear and cytoplasmatic expression in 100% normal mucosa. Using TCGA data, bisulfite pyrosequencing and CNV analysis, we excluded the possibility that loss-of-function mutations, promoter region DNA methylation or CNV are responsible for MAF loss in LSCC. Finally, we identified genes involved in the regulation of apoptosis harboring the MAF binding motif in their promoter region by applied FIMO and DAVID GO analysis. Our results highlight the role of miR-1290 in suppressing MAF expression in LSCC. Furthermore, MAF loss or mislocalization in FFPE LSCC tumor samples might suggest that MAF acts as a LSCC tumor suppressor by regulating apoptosis.</p

Dentists, members of the French Resistance movement during the World War II

The Resistance was a reaffirmation of France's independence and individuality, as well as a struggle to regain freedom and, above all, national integrity. In fact, many historians appreciate that the French Resistance could have achieved more if it had been more effectively integrated into Allied plans and strategies. Thus, in this material we tried to present some short biographies of dentists who worked in the French Resistance against the German occupation troops, some of them even paying with their lives for the courage they showed

Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation

Background Myeloid-derived suppressor cells (MDSC) are a functional myeloid cell subset that includes myeloid cells with immune suppressive properties. The presence of MDSC has been reported in the peripheral blood of patients with several malignant and non-malignant diseases. So far, direct comparison of MDSC across different diseases and Centers is hindered by technical pitfalls and a lack of standardized methodology. To overcome this issue, we formed a network through the COST Action Mye-EUNITER (www.mye-euniter.eu) with the goal to standardize and facilitate the comparative analysis of human circulating MDSC in cancer, inflammation and infection. In this manuscript, we present the results of the multicenter study Mye-EUNITER MDSC Monitoring Initiative, that involved 13 laboratories and compared circulating MDSC subsets across multiple diseases, using a common protocol for the isolation, identification and characterization of these cells. Methods We developed, tested, executed and optimized a standard operating procedure for the isolation and immunophenotyping of MDSC using blood from healthy donors. We applied this procedure to the blood of almost 400 patients and controls with different solid tumors and non-malignant diseases. The latter included viral infections such as HIV and hepatitis B virus, but also psoriasis and cardiovascular disorders. Results We observed that the frequency of MDSC in healthy donors varied substantially between centers and was influenced by technical aspects such as the anticoagulant and separation method used. Expansion of polymorphonuclear (PMN)-MDSC exceeded the expansion of monocytic MDSC (M-MDSC) in five out of six solid tumors. PMN-MDSC expansion was more pronounced in cancer compared with infection and inflammation. Programmed death-ligand 1 was primarily expressed in M-MDSC and e-MDSC and was not upregulated as a consequence of disease. LOX-1 expression was confined to PMN-MDSC. Conclusions This study provides improved technical protocols and workflows for the multi-center analysis of circulating human MDSC subsets. Application of these workflows revealed a predominant expansion of PMN-MDSC in solid tumors that exceeds expansion in chronic infection and inflammation

Vitamin D prevents endothelial progenitor cell dysfunction induced by sera from women with preeclampsia or conditioned media from hypoxic placenta

Context: Placenta-derived circulating factors contribute to the maternal endothelial dysfunction underlying preeclampsia. Endothelial colony forming cells (ECFC), a sub-population of endothelial progenitor cells (EPCs), are thought to be involved in vasculogenesis and endothelial repair. Low vitamin D concentrations are associated with an increased risk for preeclampsia. Objective: We hypothesized that the function of human fetal ECFCs in culture would be suppressed by exposure to preeclampsia-related factors-preeclampsia serum or hypoxic placental conditioned medium- in a fashion reversed by vitamin D. Design, Setting, Patients: ECFCs were isolated from cord blood of uncomplicated pregnancies and expanded in culture. Uncomplicated pregnancy villous placenta in explant culture were exposed to either 2% (hypoxic), 8% (normoxic) or 21% (hyperoxic) O2 for 48 h, after which the conditioned media (CM) was collected. Outcome Measures: ECFC tubule formation (Matrigel assay) and migration were examined in the presence of either maternal serum from preeclampsia cases or uncomplicated pregnancy controls, or pooled CM, in the presence or absence of 1,25(OH)2 vitamin D3. Results: 1,25(OH)2 vitamin D3 reversed the adverse effects of preeclampsia serum or CM from hypoxic placenta on ECFCs capillary-tube formation and migration. Silencing of VDR expression by VDR siRNA, VDR blockade, or VEGF pathway blockade reduced ECFC functional abilities. Effects of VDR or VEGF blockade were partially prevented by vitamin D. Conclusion: Vitamin D promotes the capillary-like tubule formation and migration of ECFCs in culture, minimizing the negative effects of exposure to preeclampsia-related factors. Further evaluation of the role of vitamin D in ECFC regulation and preeclampsia is warranted. © 2014 Brodowski et al