Genetic characterization of banana clones grown in Turkey based on nuclear DNA content and SRAP markers

This study was conducted to investigate the genetic relationships among banana clones grown in Turkey based on their nuclear DNA contents and SRAP markers. Four banana clones including 'Dwarf Cavendish', 'Grand Nain', 'Azman' and local 'Erdemli' were used as plant material. Nuclear DNA content of the banana cultivars estimated by flow cytometer and varied between 1.766 pg ('Erdemli') and 2.028 pg ('Grand Nain'). 'Azman' and 'Dwarf Cavendish' was similar nuclear DNA content. Genetic similarities of 4 banana clones were between 0.63-0.91 based on SRAP molecular marker. The local  'Erdemli' banana clone was the most distinct from the others. In conclusion, there is a high level of genetic variation among the banana clones grown in Turkey and the local clone 'Erdemli' is the most distinct one. This study showed that nuclear DNA content analysis together with molecular markers can be useful to assess the relationships among banana clones

Acute dystonia developing due to parenteral administration of metoclopramide: two case report

Metoklopramid kullanımına bağlı distoni olgusu literatürde sık görülmektedir. Biz burada metoklopramide bağlı erişkinde görülen iki distoni olgusu sunduk. Hastalığın erken tanı ve tedavisi hasta konforu açısından önemlidir.Dystonia case developing due to metoclopramide use is common in the literature. We presented here two adult dystonia case after use of metoclopramide. Early diagnosis and treatment of the disease is important for comfort of patient

Acil tıp hizmeti veren hekimlerde tükenme sendromu

Tükenme sendromu, bireylerin işleri gereği karşılaştıkları insanlara karşı duyarsızlaşmaları, duygusal yönden kendilerini tükenmiş hissetmeleri ve kişisel başarı duygularının azalması biçiminde tanımlanmıştır. Sonuçlan ile kurum içi hizmette verimin azalmasına sebep olan bu sendrom hem hizmet alan hem de hizmet verenleri kötü yönde etkilemektedir. İnsanlarla yakın çalışma gerektiren mesleklerde daha sık görülmektedir. Çalışmamızda tükenme ve ilişkili durumlara daha fazla maruz kaldığını düşündüğümüz acil servis hekimlerinin tükenme düzeylerini ve tükenmeye yol açan faktörleri araştırdık. 1 Temmuz ile 10 Eylül 2004 tarihleri arasında, Konya ili, çevre il ve ilçelerde bulunan Devlet, Üniversite, SSK, özel hastanelerin acil servislerinde ve 112 acil yardım istasyonlarında çalışan 246 hekim çalışmaya katılmıştır. Veriler Maslach Tükenmişlik Ölçeği (MTÖ), genel sağlık anketi 12 (GSA12) ve sosyodemografik veri formu kullanılarak elde edilmiştir. Veriler SPSS 10.0 istatistik programı ile değerlendirilmiştir. Acil servis hekimleri Maslach tükenmişlik alt ölçeği puanlan ortalamalarının Türkiye'de diğer şehirlerde yapılan benzer çalışma ortalamalarına yakın olduğu tespit edilmiştir. Çalışmamızda acil servis hekimlerinin cinsiyet, yaş, medeni durum, unvan, il ilçe bazında çalışılan yer, hekimlik yıllan, aylık çalışma süreleri, gelir düzeyleri ve sağlık durumlarındaki farklılıklarının tükenme düzeyleri üzerine etkisi olmadığı saptanmıştır. Bununla beraber 112 hekimlerinin daha az duygusal tükenme yaşadıktan saptanmış olup bu sonuç 112 hekimlerinin hastane kapsamında hizmet vermemeleri, hasta hekim ilişkisi sürelerinin nispeten kısa olmasına bağlanmıştır. Acil serviste verilen hizmet süresinin artması ile duygusal tükenmenin arttığı ayrıca hekimlerin acil serviste çalışmak istediği sürelerin, tükenme düzeyleri ile doğrudan ilişkili olduğu belirlenmiştir. Acil serviste tutulan nöbetle ilişkili faktörlerin tükenmeye yol açtığı saptanmıştır. Bir ayda tutulan nöbet sayısının az veya çok olmasının, nöbette bakılan hasta sayısının fazlalığıma, nöbette istirahat edememenin, nöbet süresinin uzunluğunun ve hekimlerin hasta ve hasta yakınları tarafından saldırıya maruz kalmalarının tükenme düzeylerini artırdığı tespit edilmiştir. Bununla beraber hekimlerin sigara, alkol gibi madde kullanımları sorulmuş olup tükenme üzerinde duygusal tükenme puanlan ile doğru orantılı bulunmuştur. GSA 12 ruhsal sağlık tarama testi ile tükenme düzeyleri üzerinde zayıf ilişkili korelasyon saptanmış olup hasta olduğunu belirten ve günlük sigara kullanım adetinin fazla olduğu hekimlerde olası ruhsal hastalık varlığının yüksek olduğu saptanmıştır..Burnout syndrome can be defined as depersonalization (loss of the sensitization to the individuals due to the nature of work), feeling emotionaly exhausted, and a low sense of personal accomplishment. Related to such consequence as decreased the efficiency of work the both of working stuff and clients suffer from this syndrome. Especially at the jobs closely working with people it is seen. Between 1 June and 10 September, 246 physicians had been taken into the study, working in the emergency rooms of State hospitals, Social Security Institude Hospital, University and private hospitals and the stations of 112. Data were obtained by using Maslach's Burnout Scale, General Health Questionaire 12 and social demografik survey form. The mean scores of our study is similar to the other studies performed to physicians in our country. Our study showed that there is no significant difference on the burnout scores by the variables of age, maritual status, gender, career, working in town or city, the past time on the medicine job, health situation of physicians and the salary. The low emotional exhaustion scores of 112 station physicians explained by the shortness of the doctor patient relation time and the low capacity of occupational management due to the hospitals. Increasing the past time experience and the willingness to work in the emergency room is directly correlated the high levels of emotional exhaution. The factors related with the night shift causes the high levels of burnout. These factors are amount of night shifts in a month, the resting time on shifts, the patient load, the night shift time and the violance in the emergency room. On the other hand substance using as the coping method of physicians was asked and found the high levels of emotional exhaution on the users. It was founded the low correlation between general health questionarrie 12 and the levels of burnout. The high probablity of having mental illness was obtained by the confessions of physicians about health status and smoking habbit

Individual information to patients with type two diabetes : A litterature study about patients experiences about information from the healthcare

Bakgrund: Diabetes typ 2 är en av de stora folksjukdomarna i västvärlden och numer även i utvecklingsländerna. Då patienterna är i behov av korrekt information för att lära sig att hantera och leva med sjukdomen är det viktigt att sjuksköterskor har kunskap om hur patienterna upplever information från vården. Syfte: Syftet var att utforska vad för erfarenheter patienter med diabetes typ två har av information från vården.  Metod: En litteraturstudie som är baserad på tidigare forskning om diabetes typ två och information. Tolv vetenskapliga artiklar med kvalitativ ansats har analyserats med textanalys. Resultat: Resultatet i denna uppsats visar att patienterna efterlyser information som är anpassad till dem som unika individer och som är lätt att förstå och hantera, då de behöver verktyg för sin egenvård. Fyra teman som framkom: Svårigheter att ta emot och anpassa sig efter information, Information anpassad till att klara av dagligt liv, Skillnader på information från läkaren och sjuksköterskan och Anpassad information. Diskussion: Resultatet diskuteras bl a i förhållande till Orems teori kring egenvård. Då patienterna ofta har svårt att anpassa till sin sjukdom behöver de stöd i form av information för att klara av att leva ett så normalt liv som möjligt. Detta blir allt viktigare då diabetes typ två är en sjukdom som kryper allt lägre ner i åldrarna och det därmed är något man ska leva länge med.Background: Diabetes type 2 is one of the largest forms of lifestyle disease in the developed countries, but in later years, also in development countries. Patients are in need of accurate information to learn how to manage and live with the disease. Therefore it is important that nurses have knowledge of how patients perceive information from health care.  Aim: The aim of the present study was to investigate what experiences patients with diabetes type two has regarding information from health care. Method: A literature study based on previous research regarding diabetes type two and information to patients. Twelve scientific articles with qualitative approach was analysed with text analysis. Result: The results show that the patients call for information that is tailored to them as unique individuals and that is easy to understand and adapt to, as they need tools for their self-care. Four themes emerged: Difficulties to accept and adapt to information, Information adapted to cope with daily life, Differences in information from the physician and the nurse and Accessible information. Discussion: The results are discussed in relation to Orem theory of self-care. Since patients often have difficulty adjusting to their disease, they need support in terms of information to be able to live as normal a life as possible. This becomes increasingly important, as diabetes type two is a disease that younger and younger ages, and it is therefore something they have to live a long time with

Individual information to patients with type two diabetes : A litterature study about patients experiences about information from the healthcare

Bakgrund: Diabetes typ 2 är en av de stora folksjukdomarna i västvärlden och numer även i utvecklingsländerna. Då patienterna är i behov av korrekt information för att lära sig att hantera och leva med sjukdomen är det viktigt att sjuksköterskor har kunskap om hur patienterna upplever information från vården. Syfte: Syftet var att utforska vad för erfarenheter patienter med diabetes typ två har av information från vården.  Metod: En litteraturstudie som är baserad på tidigare forskning om diabetes typ två och information. Tolv vetenskapliga artiklar med kvalitativ ansats har analyserats med textanalys. Resultat: Resultatet i denna uppsats visar att patienterna efterlyser information som är anpassad till dem som unika individer och som är lätt att förstå och hantera, då de behöver verktyg för sin egenvård. Fyra teman som framkom: Svårigheter att ta emot och anpassa sig efter information, Information anpassad till att klara av dagligt liv, Skillnader på information från läkaren och sjuksköterskan och Anpassad information. Diskussion: Resultatet diskuteras bl a i förhållande till Orems teori kring egenvård. Då patienterna ofta har svårt att anpassa till sin sjukdom behöver de stöd i form av information för att klara av att leva ett så normalt liv som möjligt. Detta blir allt viktigare då diabetes typ två är en sjukdom som kryper allt lägre ner i åldrarna och det därmed är något man ska leva länge med.Background: Diabetes type 2 is one of the largest forms of lifestyle disease in the developed countries, but in later years, also in development countries. Patients are in need of accurate information to learn how to manage and live with the disease. Therefore it is important that nurses have knowledge of how patients perceive information from health care.  Aim: The aim of the present study was to investigate what experiences patients with diabetes type two has regarding information from health care. Method: A literature study based on previous research regarding diabetes type two and information to patients. Twelve scientific articles with qualitative approach was analysed with text analysis. Result: The results show that the patients call for information that is tailored to them as unique individuals and that is easy to understand and adapt to, as they need tools for their self-care. Four themes emerged: Difficulties to accept and adapt to information, Information adapted to cope with daily life, Differences in information from the physician and the nurse and Accessible information. Discussion: The results are discussed in relation to Orem theory of self-care. Since patients often have difficulty adjusting to their disease, they need support in terms of information to be able to live as normal a life as possible. This becomes increasingly important, as diabetes type two is a disease that younger and younger ages, and it is therefore something they have to live a long time with

Theoretical molecular structure, vibrational frequencies and NMR investigations of 2-[(1E)-2-aza-2-(5-methyl(2-pyridyl)ethenyl)]-4-bromobenzen-1-ol

The normal mode frequencies and corresponding vibrational assignments, ¹H and ¹³C NMR chemical shifts and structural parameters (bond lengths, bond and dihedral angles) of 2-[(1E)-2-aza-2-(5-methyl(2-pyridyl)ethenyl)]-4-bromobenzen-1-ol (2mpe-4bb) Schiff base compound have been theoretically examined by means of Hartree-Fock (HF) and Becke-3-Lee-Yang-Parr (B3LYP) density functional methods with 6-31G(d) and 6-311++G(d,p) basis sets. Furthermore, reliable vibrational assignments have made on the basis of potential energy distribution (PED) calculated and the thermodynamics functions, highest occupied and lowest unoccupied molecular orbitals (HOMO and LUMO) of 2mpe-4bb have been predicted. Theoretical results have been successfully compared with available experimental data in the literature. Regarding the calculations, 2mpe-4bb prefers enol-imine form and DFT method is superior to HF approach except for predicting bond lengths.DOI: http://dx.doi.org/10.4314/bcse.v26i2.1

Effects of confluency, roscovitine and serum starvation on the cell-cycle synchronization and viability of sheep and goat adult fibroblasts

European Biotechnology Congress -- MAY 15-18, 2014 -- Lecce, ITALY[No Abstract Available

Synchronization of fresh and frozen granulosa cells obtained from water buffalo and bovine

European Biotechnology Conference -- MAY 05-07, 2016 -- LATVIA[No Abstract Available