Supramolecular Self-Assembly, DNA interaction, Antibacterial and Cell Viability studies of Cu(II) and Ni(II) Complexes derived from NNN donor Schiff Base ligand

In the present study, synthesis of two complexes, namely [Cu(L)Cl2] (1) and [Ni(L)Cl(H2O)2)]Cl (2), where L = piperidin-2-yl-N-(1-(pyridin-2-yl) ethylidene)methanamine were reported along with their characterization by spectroscopic techniques. The crystal structures were elucidated by single crystal X-ray diffraction technique. The structures of the complexes showed square pyramidal geometry for Cu(II) and octahedral geometry for Ni(II) centers. Different characterization techniques including electronic absorption spectroscopy, viscosity measurements and fluorescence spectroscopy were used to study the binding interaction of complex 1 and 2 with Calf thymus DNA (CT-DNA). The result reflected that both the complexes were able to exhibit DNA binding potential by intercalation pathway. Study of antibacterial activity using the bacterial strain E. coli showed that only complex 1 exhibited antibacterial property. Besides, metal, ligand and its complexes were evaluated individually for cell viability studies through MTT assay of HeLa cells. It is observed that cell viability increases with time for all the systems illustrating biocompatible nature of metals, ligands and their complexes. Among the complexes 1 and 2, complex 1 is suitable for higher cell viability

Synthesis, structure, and biological properties of a Co(II) complex with tridentate Schiff base ligand

<p>A new Co(II) complex of general formula [Co(L)₂] has been synthesized from a NNO tridentate Schiff base ligand, 2-[(piperidin-2-ylmethylimino)-methyl]-phenol (L). The title complex is characterized by elemental, spectroscopic, antibacterial, and single crystal X-ray structural studies. X-Ray crystallography reveals that the complex shows a distorted octahedral geometry around the Co(II) ion. The complex was tested against several bacteria and shows good antibacterial activities against almost all of the bacteria. The interactions of the title complex with calf thymus deoxyribonucleic acid (CT-DNA) have been investigated by electronic absorption and fluorescence spectroscopy, showing that the complex interacts with CT-DNA via partial intercalation. Thermogravimetric analysis (TGA) of the complex has also been reported and the result shows that the complex is thermally stable up to 134 °C.</p

Biophysical insights into the binding capability of Cu(II) schiff base complex with BSA protein and cytotoxicity studies against SiHa

Herein, we have explored the effects of chlorinated mononuclear Cu(II) complex upon binding with BSA protein (bovine serum albumin) and its in vitro anti-proliferative potentiality against SiHa cell. The complex was synthesized involving a Schiff base ligand having N,N,O donor centers and characterized by several spectroscopic studies. Structure, DFT studies and Hirshfeld surface (HS) analyses were identified using crystallographic computational studies. The binding interaction with BSA depicts the efficacy of the complex towards promising binding of it with BSA. Further, the complex shows a moderate cytotoxicity against SiHa cancer cell signifying its potentiality as an anti-proliferative agent for human cervix uteri carcinoma.publishe

A multi-spectroscopic and molecular docking approach for DNA/protein binding study and cell viability assay of first-time reported pendent azide bearing Cu(II)-quercetin and dicyanamide bearing Zn(II)-quercetin complexes

In the current study, one new quercetin-based Zn(II) complex [Zn(Qr)(CNNCN)(H2O)2] (Complex 1) which is developed by condensation of quercetin with ZnCl2 in the presence of NaN(CN)2 and Cu(II) complex [Cu(Qr)N3(CH3OH)(H2O)] (complex 2) which is developed by the condensation reaction of quercetin and CuCl2 in presence of NaN3, are thoroughly examined in relation to their use in biomedicine. The results of several spectroscopic studied confirm the structure of both the complexes and the Density Functional Theory (DFT) study helps to optimize the structure of complex 1 and 2. After completion of the identification process, DNA and Human Serum Albumin (HSA) binding efficacy of both the investigated complexes are performed by implementing a long range of biophysical studies and a thorough analysis of the results unveils that complex 1 has better interaction efficacy with the macromolecules than complex 2. The binding efficacy of complex 1 is comparatively higher towards both macromolecules because of its pure groove binding mode during interaction with DNA and the presence of an extra H-bond during connection with HSA. The experimental host-guest binding results is fully validated by molecular docking study. Interestingly complex 1 shows better antioxidant properties than complex 2, as well as quercetin, and it has strong anticancer property with minimal damage to normal cells, which is proved by the MTT assay study. Better DNA and HSA binding efficacy of 1 may be the reason for the better anticancer property of complex 1

The designed synthesis, recognition, and possible applications of Zn(III) complex

The structural pressure-induced ionization process is implemented to produce Zn(III) complex for the first time and possible potential applications for technological advances are reported in this article. The inversion of the electron population observed at the ground state of the newly synthesized complex corroborates an unexplored material response property towards electrical and magnetic fields. The first-time report of a true transition metal behavior of zinc fetches new thoughts about zinc-based bio-enzymatic and bio-catalytic processes along with its material applications in untouched files like live cell imaging contrast agents, photocatalytic water splitting, etc. The methodology of the ‘ structural pressure-induced ionization process’ may be implemented for the synthesis of more unusual oxidation states of metals