Can shed snakeskin be considered to be a model membrane for human stratum corneum?

Recently there has been some interest in the use of shed snake skin as a "model" membrane for in vitro diffusion studies. Many different species of snake have been utilised as well as different skin sites (dorsal and ventral). The species is usually named and sometimes the skin site is indicated butsometimes neither species nor skin site is reported. Insome countries it is particularly difficult to obtain human skin for in vitro experimentation and it is therefore important to have alternate biological or synthetic membranes which mimic human skin membranes for diffusion experiments. In South Africa. shed snake skin is easily obtainable from the many snake parks present in the country. Since snakes moult periodically, a single animal can provide repeated sheds, thus reducing interindividual variability. Skins can be obtained without injury to the animal and do not have to be subjected to chemical or heat stress prior to use. The epidermis is shed as a large intact sheet, thus a single snake skin can provide multiple samples. Shed snake skin is not a living tissue, can be stored for long periods at room temperature and is easily transported. Stored and fresh snake skins appear to show no differences in permeability. Since snake skin lacks hair follicles,the problems associated with the transfollicular route of penetration, which may be significant in mammalian skins, can be avoided

In vitro permeation of progesterone from a gel through the shed skin of three different snake species

The in vitro diffusion of progesterone from a gel formulation using the European Pharmacopoeia method for transdermal dosage forms is described. The membranes used were the dorsal and ventral portions of the shed skin of three different species of snake. Considerable differences are apparent between the dorsal and ventral sites and between the different species of snake. The dorsal area shows better permeability for progesterone and the permeability order for the different species is python>cobra>viper. These differences may be due to the thickness of the skin and the hinge:scale ratio. The results indicate that shed snake skin is not a model membrane for human skin

Thermal structure and exhumation history of the Lesser Himalaya in central Nepal

The Lesser Himalaya (LH) consists of metasedimentary rocks that have been scrapped off from the underthrusting Indian crust and accreted to the mountain range over the last ~20 Myr. It now forms a significant fraction of the Himalayan collisional orogen. We document the kinematics and thermal metamorphism associated with the deformation and exhumation of the LH, combining thermometric and thermochronological methods with structural geology. Peak metamorphic temperatures estimated from Raman spectroscopy of carbonaceous material decrease gradually from 520°–550°C below the Main Central Thrust zone down to less than 330°C. These temperatures describe structurally a 20°–50°C/km inverted apparent gradient. The Ar muscovite ages from LH samples and from the overlying crystalline thrust sheets all indicate the same regular trend; i.e., an increase from about 3–4 Ma near the front of the high range to about 20 Ma near the leading edge of the thrust sheets, about 80 km to the south. This suggests that the LH has been exhumed jointly with the overlying nappes as a result of overthrusting by about 5 mm/yr. For a convergence rate of about 20 mm/yr, this implies underthrusting of the Indian basement below the Himalaya by about 15 mm/yr. The structure, metamorphic grade and exhumation history of the LH supports the view that, since the mid-Miocene, the Himalayan orogen has essentially grown by underplating, rather than by frontal accretion. This process has resulted from duplexing at a depth close to the brittle-ductile transition zone, by southward migration of a midcrustal ramp along the Main Himalayan Thrust fault, and is estimated to have resulted in a net flux of up to 150 m^2/yr of LH rocks into the Himalayan orogenic wedge. The steep inverse thermal gradient across the LH is interpreted to have resulted from a combination of underplating and post metamorphic shearing of the underplated units

Evolution of the macromolecular structure of sporopollenin during thermal degradation

AbstractReconstructing the original biogeochemistry of organic microfossils requires quantifying the extent of the chemical transformations they experienced during burial and maturation processes. In the present study, fossilization experiments have been performed using modern sporopollenin chosen as an analogue for the resistant biocompounds possibly constituting the wall of many organic microfossils. Sporopollenin powder has been processed thermally under argon atmosphere at different temperatures (up to 1000 °C) for varying durations (up to 900 min). Solid residues of each experiment have been characterized using infrared, Raman and synchrotron-based XANES spectroscopies. Results indicate that significant defunctionalisation and aromatization affect the molecular structure of sporopollenin with increasing temperature. Two distinct stages of evolution with temperature are observed: in a first stage, sporopollenin experiences dehydrogenation and deoxygenation simultaneously (below 500 °C); in a second stage (above 500 °C) an increasing concentration in aromatic groups and a lateral growth of aromatic layers are observed. With increasing heating duration (up to 900 min) at a constant temperature (360 °C), oxygen is progressively lost and conjugated carbon–carbon chains or domains grow progressively, following a log-linear kinetic behavior. Based on the comparison with natural spores fossilized within metasediments which experienced intense metamorphism, we show that the present experimental simulations may not perfectly mimic natural diagenesis and metamorphism. Yet, performing such laboratory experiments provides key insights on the processes transforming biogenic molecules into molecular fossils

Inherited Ocean-Continent Transition zones in deeply subducted terranes: Insights from Alpine Corsica

In the Schistes Lustrés of Alpine Corsica (France) serpentinized mantle rocks are associated with continental basement and meta-volcanic/-sedimentary cover rocks. The relationships among these different lithologies are especially well exposed in the Monte San Petrone unit, where Alpine metamorphism reached lawsonite-eclogite conditions. The contact between serpentinites and slivers of continental basement, relatively flat-lying over several kilometers, is characterized by evidence of cataclastic deformation pre-dating Alpine High-Pressure ductile fabrics. The serpentinite/continental basement pair is stratigraphically overlain by metasediments with a typical Jurassic-Cretaceous supra-ophiolitic lithostratigraphy, with metaradiolarites passing upward to marbles and calcschists. Noticeably, no evidence of cataclastic deformation is found in metasediments. These observations indicate that the lithostratigraphy of the Monte San Petrone unit was established during a pre-Alpine polyphase evolution, which culminated in extensive brittle deformation along a flat-lying detachment fault prior to the deposition of Jurassic sediments. We suggest that the inferred Mesozoic extensional tectonics were related to the opening of the Western Tethys. The Mesozoic architecture of the Monte San Petrone area, which is typical of an Ocean-Continent Transition (OCT) zone, was preserved despite Alpine deformation and metamorphism, when the different lithologies (i.e. meta-ophiolites, continent-derived rocks and metasediments) underwent a common metamorphic evolution, culminating at T=490-550. °C and P=2.2-2.6. GPa. Similar tectono-stratigraphic associations are observed in other high-pressure terranes of Alpine Corsica, suggesting that inherited OCT-type domains may be common in Alpine-type orogens. © 2011 Elsevier B.V

Understanding The Correlation Of Libs And Acoustic Measurements Of Rocks And Soils Found In The Traverse Of The Perseverance Rover Across The Jezero Crater, Mars

The SuperCam instrument of the NASA MARS 2020 Perseverance rover combines a suite of atomic and molecular spectroscopies intended for an extensive description of rocks, soils and minerals in the surroundings of the landing site of the mission – the Jezero crater. The microphone installed on the SuperCam instrument allows the acquisition of acoustic signals resulting from the expansion of laser-induced plasmas towards the atmosphere. Apart from being affected by the propagation characteristics of the Mars atmosphere, the acoustic signal has an additional component related to the properties of the target including surface morphology, hardness, deformation parameters, and elasticity, among others. This information is currently being investigated as a complementary resource for characterization of the ablated material and may well supplement the LIBS data gathered from coincident laser shots. This talk will present SuperCam acoustic data of rocks and minerals found in the traverse of the Perseverance rover and will discuss its correlation with LIBS spectra.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

The influence of sediment cover variability on long‐term river incision rates: An example from the Peikang River, central Taiwan

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/94660/1/jgrf824.pd

The SuperCam Remote Sensing Instrument Suite for Mars 2020

International audienceThe Mars 2020 rover, essentially a structural twin of MSL, is being built to a) characterize the geology and history of a new landing site on Mars, b) find and characterize ancient habitable environments, c) cache samples for eventual return to Earth, and d) demonstrate in-situ production of oxygen needed for human exploration. Remote-sensing instrumentation is needed to support the first three of these goals [1]. The SuperCam instrument meets these needs with a range of instrumentation including the highest-resolution remote imaging on the rover, two different techniques for determining mineralogy , and one technique to provide elemental compositions. All of these techniques are co-boresighted, providing rapid comprehensive characterization. In addition, for targets within 7 meters of the rover the laser shock waves brush away the dust, providing cleaner surfaces for analysis. SuperCam will use an advanced version of the AEGIS robotic target selection software

Synthesis of an ordered mesoporous carbon with graphitic characteristics and its application for dye adsorption

An ordered mesoporous carbon (OMC) was prepared by a chemical vapor deposition technique using liquid petroleum gas (LPG) as the carbon source. During synthesis, LPG was effectively adsorbed in the ordered mesopores of SBA-15 silica and converted to a graphitic carbon at 800 °C. X-ray diffraction and nitrogen adsorption/desorption data and high-resolution transmission electron microscopy (HRTEM) of the OMC confirmed its ordered mesoporous structure. The OMC was utilized as an adsorbent in the removal of dyes from aqueous solution. A commercial powder activated carbon (AC) was also investigated to obtain comparative data. The efficiency of the OMC for dye adsorption was tested using acidic dye acid orange 8 (AO8) and basic dyes methylene blue (MB) and rhodamine B (RB). The results show that adsorption was affected by the molecular size of the dye, the textural properties of carbon adsorbent and surface-dye interactions. The adsorption capacities of the OMC for acid orange 8 (AO8), methylene blue (MB) and rhodamine B (RB) were determined to be 222, 833, and 233 mg/g, respectively. The adsorption capacities of the AC for AO8, MB, and RB were determined to be 141, 313, and 185 mg/ g, respectively. The OMC demonstrated to be an excellent adsorbent for the removal of MB from wastewater.Web of Scienc