444 research outputs found
Diploid and tetraploid African Barbus (Osteichthyes, Cyprinidae) : on the coding of differential gene expression
Trois espĂšces du groupe des "petits" barbeaux d'Afrique de l'Ouest ont Ă©tĂ© analysĂ©s par Ă©lectrophorĂšse des protĂ©ines enzymatiques afin d'estimer leur diffĂ©renciation gĂ©nĂ©tique. La comparaison avec une espĂšce de "grand" barbeau de la mĂȘme rĂ©gion a montrĂ© que les "petits" barbeaux seraient diploĂŻdes et les "grands" tĂ©traploĂŻdes. Ils constituent certainement deux lignĂ©es distinctes. Des arbres phĂ©nĂ©tiques (distances de Nei) et cladistiques (rĂ©seaux de compatibilitĂ©) du genre #Barbus$ sont proposĂ©s comprenant les trois espĂšces diploĂŻdes africaines, deux espĂšces diploĂŻdes d'Arabie et d'Asie du sud-est, une espĂšce tĂ©traploĂŻdes de France. Ces arbres prĂ©sentent deux ensembles : les diploĂŻdes d'un cĂŽtĂ© et les tĂ©traploĂŻdes de l'autre. Plusieurs mĂ©thodes de traitement des donnĂ©es sont proposĂ©es pour rĂ©soudre les difficultĂ©s dues au traitement simultanĂ© d'espĂšces Ă niveau de ploĂŻdie diffĂ©rent. (RĂ©sumĂ© d'auteur
Postsynaptic targets of Purkinje cell terminals in the cerebellar and vestibular nuclei of the rat
Morphologic and genetic characterisation of Corsican and Sardinian trout with comments on Salmo taxonomy
Both morphological and molecular data are presented and discussed for indigenous Salmo sp. from Corsica and Sardinia, here called Tyrrhenian trout. For comparison, morphological data obtained from museum specimens, including the Algerian S. macrostigma, are discussed in the light of recent and new molecular findings. In total, 29 measurements and 20 meristic characters were taken from each specimen. Out of the meristic characters, 12 were obtained by means of X-ray. One important morphometric character in the present study is the size of the head measured from premaxilla to posterior margin of preoperculum. This character was particularly stable in all Tyrrhenian trout, showing relatively large head compared to Atlantic trout and to S. macrostigma. On the contrary, other characters like body punctuations, black and white edges of fins, body depth or number of epurals in the caudal skeleton are quite polymorphic. In certain meristic characters, range of variation of Tyrrhenian trout even exceeds that of the extensive comparative material. Each trout has been genetically characterized. New haplotypes from Tyrrhenian trout were discovered, belonging to three mitochondrial lineages viz. Adriatic, marble and Mediterranean, however, Adriatic haplotypes are dominant. Comparing morphological and genetic data, observed morphology lacks any obvious correlation to mitochondrial lineages and it is concluded that Tyrrhenian trout show no particular affinity to S. macrostigma from Algeria
Neuropeptide AF and FF modulation of adipocyte metabolism. Primary insights from functional genomics and effects on beta-adrenergic responsiveness.
The presence of a neuropeptide AF and FF receptor (NPFF-R2) mRNA in human adipose tissue (Elshourbagy, N. A., Ames, R. S., Fitzgerald, L. R., Foley, J. J., Chambers, J. K., Szekeres, P. G., Evans, N. A., Schmidt, D. B., Buckley, P. T., Dytko, G. M., Murdock, P. R., Tan, K. B., Shabon, U., Nuthulaganti, P., Wang, D. Y., Wilson, S., Bergsma, D. J., and Sarau, H. M. (2000) J. Biol. Chem. 275, 25965-25971) suggested these peptides, principally recognized for their pain modulating effects, may also impact on adipocyte metabolism, an aspect that has not been explored previously. Our aim was thus to obtain more insights into the actions of these peptides on adipocytes, an approach initially undertaken with a functional genomic assay. First we showed that 3T3-L1 adipocytes express both NPFF-R1 and NPFF-R2 transcripts, and that NPAF binds adipocyte membranes with a nanomolar affinity as assessed by surface plasmon resonance technology. Then, and following a 24-h treatment with NPFF or NPAF (1 microm), we have measured using real-time quantitative reverse transcriptase-PCR the mRNA steady state levels of already well characterized genes involved in key pathways of adipose metabolism. Among the 45 genes tested, few were modulated by NPFF ( approximately 10%) and a larger number by NPAF ( approximately 27%). Interestingly, NPAF increased the mRNA levels of beta2- and beta3-adrenergic receptors (AR), and to a lesser extent those of beta1-ARs. These variations in catecholamine receptor mRNAs correlated with a clear induction in the density of beta2- and beta3-AR proteins, and in the potency of beta-AR subtype-selective agonists to stimulate adenylyl cyclase activity. Altogether, these data show that NPFF-R1 and NPFF-R2 are functionally present in adipocytes and suggest that besides their well described pain modulation effects, NPAF and to a lesser extent NPFF, may have a global impact on body energy storage and utilization
Chapter 3 - Mobility on demand (MOD) and mobility as a service (MaaS): early understanding of shared mobility impacts and public transit partnerships
Technology is changing the way we move and reshaping cities and society. Shared and on-demand mobility represent notable transportation shifts in the 21st century. In recent years, mobility on demand (MOD)âwhere consumers access mobility, goods, and services on-demand by dispatching shared modes, courier services, public transport, and other innovative strategiesâhas grown rapidly due to technological advancements; changing consumer preferences; and a range of economic, environmental, and social factors. New attitudes toward sharing, MOD, and mobility as a service (MaaS) are changing traveler behavior and creating new opportunities and challenges for public transportation. This chapter discusses similarities and differences between the evolving concepts of MaaS and MOD. Next, it characterizes the range of existing public transit and MOD service models and enabling partnerships. The chapter also explores emerging trends impacting public transportation. While vehicle automation could result in greater public transit competition in the future, it could also foster new opportunities for transit enhancements (e.g., microtransit services, first- and last-mile connections, reduced operating costs). The chapter concludes with a discussion of how MOD/MaaS partnerships and automation could enable the public transit industry to reinvent itself, making it more attractive and competitive with private vehicle ownership and use
Environmental variables, habitat discontinuity and life history shaping the genetic structure of Pomatoschistus marmoratus
Coastal lagoons are semi-isolated ecosystems
exposed to wide fluctuations of environmental conditions
and showing habitat fragmentation. These features may
play an important role in separating species into different
populations, even at small spatial scales. In this study, we
evaluate the concordance between mitochondrial (previous
published data) and nuclear data analyzing the genetic
variability of Pomatoschistus marmoratus in five localities,
inside and outside the Mar Menor coastal lagoon (SE
Spain) using eight microsatellites. High genetic diversity
and similar levels of allele richness were observed across
all loci and localities, although significant genic and
genotypic differentiation was found between populations
inside and outside the lagoon. In contrast to the FST values
obtained from previous mitochondrial DNA analyses
(control region), the microsatellite data exhibited significant
differentiation among samples inside the Mar Menor
and between lagoonal and marine samples. This pattern
was corroborated using Cavalli-Sforza genetic distances.
The habitat fragmentation inside the coastal lagoon and
among lagoon and marine localities could be acting as a
barrier to gene flow and contributing to the observed
genetic structure. Our results from generalized additive
models point a significant link between extreme lagoonal
environmental conditions (mainly maximum salinity) and
P. marmoratus genetic composition. Thereby, these environmental
features could be also acting on genetic structure
of coastal lagoon populations of P. marmoratus favoring
their genetic divergence. The mating strategy of P. marmoratus
could be also influencing our results obtained from
mitochondrial and nuclear DNA. Therefore, a special
consideration must be done in the selection of the DNA
markers depending on the reproductive strategy of the
species
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Bridging the gap between basic science and clinical practice: The role of organizations in addressing clinician barriers
New National Institutes of Health policies call for expansion of practice-based research to improve the clinical research enterprise and facilitate dissemination of evidence-based medicine. This paper describes organizational strategies that influence clinicians' decisions to participate in clinical research. We reviewed the literature and interviewed over 200 clinicians and stakeholders. The most common barriers to community clinician participation in clinical research relate to beliefs that clinical research is too burdensome and has little benefit for the participating clinician or patient. We identified a number of approaches healthcare organizations can use to encourage clinicians to participate in research, including an outreach campaign to promote the benefits of clinical research; selection of study topics of interest to clinicians; establishment and enforcement of a set of research principles valuing the clinician and patient; development of a transparent schedule of reimbursement for research tasks; provision of technological and technical assistance to practices as needed; and promotion of a sense of community among clinicians involved in practice-based research. Many types of existing healthcare organizations could provide the technical and intellectual assistance community clinicians need to participate in clinical research. Multiple approaches are possible
Prenatal Treatment for Serious Neurological Sequelae of Congenital Toxoplasmosis: An Observational Prospective Cohort Study
Background: The effectiveness of prenatal treatment to prevent serious neurological sequelae (SNSD) of congenital toxoplasmosis is not known.Methods and Findings: Congenital toxoplasmosis was prospectively identified by universal prenatal or neonatal screening in 14 European centres and children were followed for a median of 4 years. We evaluated determinants of postnatal death or SNSD defined by one or more of functional neurological abnormalities, severe bilateral visual impairment, or pregnancy termination for confirmed congenital toxoplasmosis. Two-thirds of the cohort received prenatal treatment (189/293; 65%). 23/293 (8%) fetuses developed SNSD of which nine were pregnancy terminations. Prenatal treatment reduced the risk of SNSD. The odds ratio for prenatal treatment, adjusted for gestational age at maternal seroconversion, was 0.24 (95% Bayesian credible intervals 0.07-0.71). This effect was robust to most sensitivity analyses. The number of infected fetuses needed to be treated to prevent one case of SNSD was three (95% Bayesian credible intervals 2-15) after maternal seroconversion at 10 weeks, and 18 (9-75) at 30 weeks of gestation. Pyrimethamine-sulphonamide treatment did not reduce SNSD compared with spiramycin alone (adjusted odds ratio 0.78, 0.21-2.95). The proportion of live-born infants with intracranial lesions detected postnatally who developed SNSD was 31.0% (17.0%-38.1%).Conclusion: The finding that prenatal treatment reduced the risk of SNSD in infected fetuses should be interpreted with caution because of the low number of SNSD cases and uncertainty about the timing of maternal seroconversion. As these are observational data, policy decisions about screening require further evidence from a randomized trial of prenatal screening and from cost-effectiveness analyses that take into account the incidence and prevalence of maternal infection
Lymphocytic colitis presenting as difficult diarrhoea in an African woman: a case report and review of the literature
<p>Abstract</p> <p>Introduction</p> <p>Lymphocytic colitis is an uncommon intestinal disorder that presents with chronic diarrhoea. It is treatable, but in the developing world, its diagnosis may often prove difficult. Data and reports of this condition in Africa are scarce because most medical centres lack a functional gastrointestinal endoscopy unit that would aid in the diagnosis.</p> <p>Case presentation</p> <p>We present the case of a 53-year-old Nigerian woman with pathogen-negative chronic diarrhoea and a family history of chronic diarrhoea. She responded well to treatment after colonoscopy and colonic biopsy successfully diagnosed her illness.</p> <p>Conclusion</p> <p>Referral of patients with pathogen-negative chronic diarrhoea to medical centres that have facilities for colonoscopy and biopsy is important in the developing world.</p
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