Fluxes of Nitrogen, Phosphorus, and Dissolved Organic Carbon in the inflow of the Lehnmühle reservoir (Saxony) as compared to streams draining three main land-use types in the catchment

The aim of this study was to assess the impact of land-use on inputs of nitrogen, phosphorus, and DOC into the inflow of the Lehnmühle reservoir (drinking water supply). Land-use in the study area is dominated by forest, with smaller proportions of grassland and crops. Water quality was analyzed for the hydrological years 2010 and 2011 at the outlets of three small catchments with homogenous land-use (crops, grassland and forest) and at the outlet of the watershed. The highest nitrogen and phosphorus concentrations were observed in the streams draining the agricultural areas, and the lowest concentrations were found in the forest catchment. The DOC concentration was highest at the outlet of the watershed whereas the concentrations in the small homogeneous catchments were lower. The information collected about the land-use dependent matter exports in these study areas will be used for climate change impact modeling with the Soil and Water Assessment Tool

Learning filter functions in regularisers by minimising quotients

Learning approaches have recently become very popular in the field of inverse problems. A large variety of methods has been established in recent years, ranging from bi-level learning to high-dimensional machine learning techniques. Most learning approaches, however, only aim at fitting parametrised models to favourable training data whilst ignoring misfit training data completely. In this paper, we follow up on the idea of learning parametrised regularisation functions by quotient minimisation as established in [3]. We extend the model therein to include higher-dimensional filter functions to be learned and allow for fit- and misfit-training data consisting of multiple functions. We first present results resembling behaviour of well-established derivative-based sparse regularisers like total variation or higher-order total variation in one-dimension. Our second and main contribution is the introduction of novel families of non-derivative-based regularisers. This is accomplished by learning favourable scales and geometric properties while at the same time avoiding unfavourable ones

Identification of black sturgeon caviar pigment as eumelanin

Reported herein is the purification of the pigment of black sturgeon caviar and its unambiguous identification as a typical eumelanin by means of chemical degradation coupled with electron paramagnetic resonance (EPR) evidence. HPLC and LC-MS analysis of oxidative degradation mixtures revealed the formation of pyrrole-2,3,5-tricarboxylic acid (PTCA), a specific marker of eumelanin pigments, in yields compatible with a 6.5% w/w pigment content. EPR spectral features and parameters were in close agreement with those reported for a typical natural eumelanin such as Sepia melanin from squid ink. The identification for the first time of eumelanin in a fish roe is expected to provide a novel molecular basis for the valorization of black caviar and production wastes thereof in food chemistry and diet

A combined first and second order variational approach for image reconstruction

In this paper we study a variational problem in the space of functions of bounded Hessian. Our model constitutes a straightforward higher-order extension of the well known ROF functional (total variation minimisation) to which we add a non-smooth second order regulariser. It combines convex functions of the total variation and the total variation of the first derivatives. In what follows, we prove existence and uniqueness of minimisers of the combined model and present the numerical solution of the corresponding discretised problem by employing the split Bregman method. The paper is furnished with applications of our model to image denoising, deblurring as well as image inpainting. The obtained numerical results are compared with results obtained from total generalised variation (TGV), infimal convolution and Euler's elastica, three other state of the art higher-order models. The numerical discussion confirms that the proposed higher-order model competes with models of its kind in avoiding the creation of undesirable artifacts and blocky-like structures in the reconstructed images -- a known disadvantage of the ROF model -- while being simple and efficiently numerically solvable.Comment: 34 pages, 89 figure

Macrophages target Salmonella by Lc3-associated phagocytosis in a systemic infection model

Innate immune defense against intracellular pathogens, like Salmonella, relies heavily on the autophagy machinery of the host. This response is studied intensively in epithelial cells, the target of Salmonella during gastrointestinal infections. However, little is known of the role that autophagy plays in macrophages, the predominant carriers of this pathogen during systemic disease. Here we utilize a zebrafish embryo model to study the interaction of S. enterica serovar Typhimurium with the macroautophagy/autophagy machinery of macrophages in vivo. We show that phagocytosis of live but not heat-killed Salmonella triggers recruitment of the autophagy marker GFP-Lc3 in a variety of patterns labeling tight or spacious bacteria-containing compartments, also revealed by electron microscopy. Neutrophils display similar GFP-Lc3 associations, but genetic modulation of the neutrophil/macrophage balance and ablation experiments show that macrophages are critical for the defense response. Deficiency of atg5 reduces GFP-Lc3 recruitment and impairs host resistance, in contrast to atg13 deficiency, indicating that Lc3-Salmonella association at this stage is independent of the autophagy preinitiation complex and that macrophages target Salmonella by Lc3-associated phagocytosis (LAP). In agreement, GFP-Lc3 recruitment and host resistance are impaired by deficiency of Rubcn/Rubicon, known as a negative regulator of canonical autophagy and an inducer of LAP. We also found strict dependency on NADPH oxidase, another essential factor for LAP. Both Rubcn and NADPH oxidase are required to activate a Salmonella biosensor for reactive oxygen species inside infected macrophages. These results identify LAP as the major host protective autophagy-related pathway responsible for macrophage defense against Salmonella during systemic infection

Effects of metal cation substitution on hexavalent chromium reduction by green rust

Chromium contamination is a serious environmental issue in areas affected by leather tanning and metal plating, and green rust sulfate has been tested extensively as a potential material for in situ chemical reduction of hexavalent chromium in groundwater. Reported products and mechanisms for the reaction have varied, most likely because of green rust’s layered structure, as reduction at outer and interlayer surfaces might produce different reaction products with variable stabilities. Based on studies of Cr(III) oxidation by biogenic Mn (IV) oxides, Cr mobility in oxic soils is controlled by the solubility of the Cr(III)-bearing phase. Therefore, careful engineering of green rust properties, i.e., crystal/particle size, morphology, structure, and electron availability, is essential for its optimization as a remediation reagent. In the present study, pure green rust sulfate and green rust sulfate with Al, Mg and Zn substitutions were synthesized and reacted with identical chromate (CrO42−) solutions. The reaction products were characterized by X-ray diffraction, pair distribution function analysis, X-ray absorption spectroscopy and transmission electron microscopy and treated with synthetic δ-MnO2 to assess how easily Cr(III) in the products could be oxidized. It was found that Mg substitution had the most beneficial effect on Cr lability in the product. Less than 2.5% of the Cr(III) present in the reacted Mg-GR was reoxidized by δ-MnO2 within 14 days, and the particle structure and Cr speciation observed during X-ray scattering and absorption analyses of this product suggested that Cr(VI) was reduced in its interlayer. Reduction in the interlayer lead to the linkage of newly-formed Cr(III) to hydroxyl groups in the adjacent octahedral layers, which resulted in increased structural coherency between these layers, distinctive rim domains, sequestration of Cr(III) in insoluble Fe oxide bonding environments resistant to reoxidation and partial transformation to Cr(III)-substituted feroxyhyte. Based on the results of this study of hexavalent chromium reduction by green rust sulfate and other studies, further improvements can also be made to this remediation technique by reacting chromate with a large excess of green rust sulfate, which provides excess Fe(II) that can catalyze transformation to more crystalline iron oxides, and synthesis of the reactant under alkaline conditions, which has been shown to favor chromium reduction in the interlayer of Fe(II)-bearing phyllosilicates

Directional Sinogram Inpainting for Limited Angle Tomography

In this paper we propose a new joint model for the reconstruction of tomography data under limited angle sampling regimes. In many applications of Tomography, e.g. Electron Microscopy and Mammography, physical limitations on acquisition lead to regions of data which cannot be sampled. Depending on the severity of the restriction, reconstructions can contain severe, characteristic, artefacts. Our model aims to address these artefacts by inpainting the missing data simultaneously with the reconstruction. Numerically, this problem naturally evolves to require the minimisation of a non-convex and non-smooth functional so we review recent work in this topic and extend results to fit an alternating (block) descent framework. \oldtext{We illustrate the effectiveness of this approach with numerical experiments on two synthetic datasets and one Electron Microscopy dataset.} \newtext{We perform numerical experiments on two synthetic datasets and one Electron Microscopy dataset. Our results show consistently that the joint inpainting and reconstruction framework can recover cleaner and more accurate structural information than the current state of the art methods

Organic synthesis on Mars by electrochemical reduction of CO2

The sources and nature of organic carbon on Mars have been a subject of intense research. Steele et al. (2012) showed that 10 martian meteorites contain macromolecular carbon phases contained within pyroxene- and olivine-hosted melt inclusions. Here, we show that martian meteorites Tissint, Nakhla, and NWA 1950 have an inventory of organic carbon species associated with fluid-mineral reactions that are remarkably consistent with those detected by the Mars Science Laboratory (MSL) mission. We advance the hypothesis that interactions among spinel-group minerals, sulfides, and a brine enable the electrochemical reduction of aqueous CO2 to organic molecules. Although documented here in martian samples, a similar process likely occurs wherever igneous rocks containing spinel-group minerals and/or sulfides encounter brines

Physical weathering by glaciers enhances silicon mobilisation and isotopic fractionation

Glacial meltwaters export substantial quantities of dissolved and dissolvable amorphous silicon (DSi and ASi), providing an essential nutrient for downstream diatoms. Evidence suggests that glacially exported DSi is isotopically light compared to DSi in non-glaciated rivers. However, the isotopic fractionation mechanisms are not well constrained, indicating an important gap in our understanding of processes in the global Si cycle. We use rock crushing experiments to mimic subglacial physical erosion, to provide insight into subglacial isotope fractionation. Isotopically light DSi (δ30SiDSi) released following initial dissolution of freshly ground mineral surfaces (down to −2.12 ± 0.02 ‰) suggests mechanochemical reactions induce isotopic fractionation, explaining the low δ30SiDSi composition of subglacial runoff. ASi with a consistent isotopic composition is present in all mechanically weathered samples, but concentrations are elevated in samples that have undergone more intense physical grinding. These experiments illustrate the critical role of physical processes in driving isotopic fractionation and biogeochemical weathering in subglacial environments. Understanding perturbations in high latitude Si cycling under climatic change will likely depend on the response of mechanochemical weathering to increased glacial melt