Alas, Poor Ghost!

In the rational modern world, belief in the supernatural seemingly has been consigned to the worlds of entertainment and fantasy. Yet belief in other worldly phenomena, from poltergeists to telepathy, remains strong, as Gillian Bennett\u27s research shows. Especially common is belief in continuing contact with, or the continuing presence of, dead family members. Bennett interviewed women in Manchester, England, asking them questions about ghosts and other aspects of the supernatural. (Her discussion of how her research methods and interview techniques evolved is in itself valuable.) She first published the results of the study in the well-received Traditions of Belief: Women and the Supernatural, which has been widely used in folklore and women\u27s studies courses. Alas, Poor Ghost! extensively revises and expands that work. In addition to a fuller presentation and analysis of the original field research and other added material, the author, assisted by Kate Bennett, a gerontological psychologist, presents and discusses new research with a group of women in Leicester, England. Bennett is interested in more than measuring the extent of belief in other worldly manifestations. Her work explores the relationship between narrative and belief. She anticipated that her questions would elicit from her interviewees not just yes or no replies but stories about their experiences that confirmed or denied notions of the supernatural. The more controversial the subject matter, the more likely individuals were to tell stories, especially if their answers to questions of belief were positive. These were most commonly individualized narratives of personal experience, but they contained many of the traditional motifs and other content, including belief in the supernatural, of legends. Bennett calls them memorates and discusses the cultural processes, including ideas of what is a proper experience of the supernatural and a proper telling of the story, that make them communal as well as individual. These memorates provide direct and vivid examples of what the storytellers actually believe and disbelieve. In a final section, Bennett places her work in historical context through a discussion of case studies in the history of supernatural belief.https://digitalcommons.usu.edu/usupress_pubs/1058/thumbnail.jp

Tissue Inhibitor of Metalloproteinase–3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells

Over expression of Tissue Inhibitor of Metalloproteinases-3 (TIMP-3) in vascular smooth muscle cells (VSMCs) induces apoptosis and reduces neointima formation occurring after saphenous vein interposition grafting or coronary stenting. In studies to address the mechanism of TIMP-3-driven apoptosis in human VSMCs we find that TIMP-3 increased activation of caspase-8 and apoptosis was inhibited by expression of Cytokine response modifier A (CrmA) and dominant negative FAS-Associated protein with Death Domain (FADD). TIMP-3 induced apoptosis did not cause mitochondrial depolarisation, increase activation of caspase-9 and was not inhibited by over-expression of B-cell Lymphoma 2 (Bcl2), indicating a mitochondrial independent/type-I death receptor pathway. TIMP-3 increased levels of the First Apoptosis Signal receptor (FAS) and depletion of FAS with shRNA showed TIMP-3-induced apoptosis was FAS dependent. TIMP-3 induced formation of the Death-Inducing Signalling Complex (DISC), as detected by immunoprecipitation and by immunofluorescence. Cellular-FADD-like IL-1 converting enzyme-Like Inhibitory Protein (c-FLIP) localised with FAS at the cell periphery in the absence of TIMP-3 and this localisation was lost on TIMP-3 expression with c-FLIP adopting a perinuclear localisation. Although TIMP-3 inhibited FAS shedding, this did not increase total surface levels of FAS but instead increased FAS levels within localised regions at the cell surface. A Disintegrin And Metalloproteinase 17 (ADAM17) is inhibited by TIMP-3 and depletion of ADAM17 with shRNA significantly decreased FAS shedding. However ADAM17 depletion did not induce apoptosis or replicate the effects of TIMP-3 by increasing localised clustering of cell surface FAS. ADAM17-depleted cells could activate caspase-3 when expressing levels of TIMP-3 that were otherwise sub-apoptotic, suggesting a partial role for ADAM17 mediated ectodomain shedding in TIMP-3 mediated apoptosis. We conclude that TIMP-3 induced apoptosis in VSMCs is highly dependent on FAS and is associated with changes in FAS and c-FLIP localisation, but is not solely dependent on shedding of the FAS ectodomain

The challenges, uncertainties and opportunities of bioaerosol dispersion modelling from open composting facilities

Bioaerosols are ubiquitous organic particles that comprise viruses, bacteria and coarser fractions of organic matter. Known to adversely affect human health, the impact of bioaerosols on a population often manifests as outbreaks of illnesses such as Legionnaires Disease and Q fever, although the concentrations and environmental conditions in which these impacts occur are not well understood. Bioaerosol concentrations vary from source to source, but specific human activities such as water treatment, intensive agriculture and composting facilitate the generation of bioaerosol concentrations many times higher than natural background levels. Bioaerosols are not considered ‘traditional’ pollutants in the same way as PM10, PM2.5, and gases such as NO2, and consequently dispersion models do not include a bespoke method for their assessment. As identified in previous studies, priority areas for improving the robustness of these dispersion models include: 1) the development of bespoke monitoring studies designed to generate accurate modelling input data; 2) the publication of a robust emissions inventory; 3) a code of practice to provide guidelines for consistent bioaerosol modelling practices; and 4) a greater understanding of background bioaerosol emissions. The aim of this research project, funded by the Natural Environmental Research Council (NERC), is to address these key areas through a better understanding of the generation, concentration and potential dispersion of bioaerosols from intensive agricultural and biowaste facilities, using case studies developed at specific locations within the UK. The objective is to further refine existing bioaerosol monitoring and modelling guidelines to provide a more robust framework for regulating authorities and site operators. This contribution outlines the gaps that hinder robust dispersion modelling, and describes the on-site bioaerosol data collection methods used in the study, explaining how they might be used to close these gaps. Examples of bioaerosol dispersion modelled using ADMS 5 are presented and discussed

Rehabilitation nurses’ knowledge, experiences, and perceptions of the provision of psychological care for patients post stroke in Ireland: A cross-sectional study.

Introduction: Early assessment and management of depression and anxiety for patients after stroke is critical. However, suboptimal provision of psychological care is widely reported. Nurses are the largest professional group to care for patients post stroke and have an important role in addressing their emotional wellbeing. Aim: The aim of this paper is to examine nurse’s knowledge, experiences, and perceptions of the provision of psychological care to patients post stroke in Ireland. Methods: A cross-sectional descriptive survey design involving both quantitative and qualitative items was administered to a convenience sample of seventy-four (n=74) nurses working in six Irish community hospital rehabilitation units. Results: Findings demonstrate suboptimal psychological care provision for patients post stroke with no formal pre-defined care pathways. Despite nurse’s knowledge of psychological problems and symptomology, participants revealed receiving limited training in psychological monitoring and no systemic allocation of psychological care duties. This research suggests that pre-defined/formal care pathways; access to psychology expertise; further training and support for members of the Multi-Disciplinary-Team has the potential to improve the provision of psychological care for patients post stroke. Conclusions: This study has implications for the reform and development of rehabilitation services in relation to practice, education, and research. It identifies opportunities to support nurses to improve the delivery of psychological care for patients post stroke

Towards improved bioaerosol model validation and verification

Bioaerosols, comprised of bacteria, fungi and viruses are ubiquitous in ambient air. Known to adversely affect human health, the impact of bioaerosols on a population often manifests as outbreaks of illnesses such as Legionnaires Disease and Q fever, although the concentrations and environmental conditions in which these impacts occur are not well understood. Bioaerosol concentrations vary from source to source, but specific industrialised human activities such as water treatment, intensive agriculture and open windrow composting facilitate the generation of bioaerosol concentrations many times higher than natural background levels. Bioaerosol sampling is currently undertaken according to the requirements of the Environment Agency’s regulatory framework, in which the collection of bioaerosols and not its long-term measurement is of most importance. As a consequence, sampling devices are often moved around site according to changing wind direction and sampling intervals are invariably short-term. The dispersion modelling of bioaerosols from composting facilities typically relies on proxy pollutant parameters. In addition, the use of short term emission data gathering strategies in which monitors are moved frequently with wind direction, do not provide a robust reliable and repeatable dataset by which to validate any modelling or to verify its performance. New sampling methods such as the Spectral Intensity Bioaerosol Sensor (SIBS) provide an opportunity to address several gaps in bioaerosol model validation and verification. In the context of model validation, this paper sets out the current weaknesses in bioaerosol monitoring from the perspective of robust modelling requirements

Investigating the barriers to the uptake of Diabetic RetinaScreen

Diabetic retinopathy is a significant complication of diabetes, and the most common cause of blindness in people under the age of 65. The National Diabetic Retinal Screening Programme (Diabetic RetinaScreen) was established to detect sight threatening retinopathies. The purpose of this cross-sectional study is to determine the barriers to the uptake of Diabetic RetinaScreen, to investigate discrepancies in attendance, if any, between patients whose diabetes care is delivered in a large tertiary referral hospital out-patient setting or in general practice, and to evaluate general practitioner’s satisfaction with the service. Older age (OR 1.023, 95% CI 1.001 to 1.046) and complications of diabetes, excluding ocular complications, (OR 2.741, 95% CI 1.158 to 6.489) were associated with increased attendance at Diabetic RetinaScreen. Online referral is now available and the preferred method of referral. Efforts to encourage younger patients who do not yet have complications of diabetes may be beneficial

Sources of Airborne Endotoxins in Ambient Air and Exposure of Nearby Communities—A Review

Endotoxin is a bioaerosol component that is known to cause respiratory effects in exposed populations. To date, most research focused on occupational exposure, whilst much less is known about the impact of emissions from industrial operations on downwind endotoxin concentrations. A review of the literature was undertaken, identifying studies that reported endotoxin concentrations in both ambient environments and around sources with high endotoxin emissions. Ambient endotoxin concentrations in both rural and urban areas are generally below 10 endotoxin units (EU) m−3; however, around significant sources such as compost facilities, farms, and wastewater treatment plants, endotoxin concentrations regularly exceeded 100 EU m−3. However, this is affected by a range of factors including sampling approach, equipment, and duration. Reported downwind measurements of endotoxin demonstrate that endotoxin concentrations can remain above upwind concentrations. The evaluation of reported data is complicated due to a wide range of different parameters including sampling approaches, temperature, and site activity, demonstrating the need for a standardised methodology and improved guidance. Thorough characterisation of ambient endotoxin levels and modelling of endotoxin from pollution sources is needed to help inform future policy and support a robust health-based risk assessment process