82 research outputs found
Nutritional status of HIV-infected patients during the first year HAART in two West African cohorts
Objective: To examine the association between nutritional markers at
initiation and during follow up in two different cohorts of
HIV-infected adults initiating highly active antiretroviral therapy
(HAART) in West Africa. Methods: The ATARAO study was a one year
prospective study carried in Mali. It consisted of a sample of
consecutive patients initiating HAART in one of four participating
centers during that period. Data were collected at time of treatment
initiation (baseline) and every 3 months thereafter. The ANRS 1290
study followed Senegalese patients recruited in similar conditions.
Bivariate analyses were used to identify nutritional and immunological
covariates of malnutrition at baseline. Longitudinal trajectories of
body mass index, hemoglobin and albumin, and their associated factors,
were evaluated using mixed linear models. Results: In ATARAO, 250
participants were retained for analyses; of which, 36% had a BMI <
18.5 kg/m2, nearly 60% were anemic and 47.4% hypoalbuminemic at time of
treatment initiation. At baseline, low hemoglobin, hypoalbuminemia and
low CD4 levels were associated with a BMI < 18.5 kg/m2. Similarly,
low BMI, low albumin and low CD4 counts were linked to anemia; while,
hypoalbuminemia was associated with low hemoglobin levels and CD4
counts. In ANRS, out of the 372 participants retained for analyses, 31%
had a low BMI and almost 70% were anemic. At baseline, low BMI was
associated with low hemoglobin levels and CD4 counts, while anemia was
associated with low CD4 counts and female sex. While treatment
contributed to early gains in BMI, hemoglobin and albumin in the first
6 months of treatment, initial improvements plateaued or subsided
thereafter. Despite HAART, malnutrition persisted in both cohorts after
one year, especially in those who were anemic, hypoalbuminemic or had a
low BMI at baseline. Conclusion: In ATARAO and ANRS, malnutrition was
common across all indicators (BMI, hemoglobin, albumin) and persisted
despite treatment. Low BMI, anemia and hypoalbuminemia were associated
with attrition, and with a deficient nutritional and immunological
status at baseline, as well as during treatment. In spite of therapy,
malnutrition is associated with negative clinical and treatment
outcomes which suggests that HAART may not be sufficient to address
co-existing nutritional deficiencies
Alterations of Blood Brain Barrier Function in Hyperammonemia: An Overview
Ammonia is a neurotoxin involved in the pathogenesis of neurological conditions associated with hyperammonemia, including hepatic encephalopathy, a condition associated with acute—(ALF) or chronic liver failure. This article reviews evidence that apart from directly affecting the metabolism and function of the central nervous system cells, ammonia influences the passage of different molecules across the blood brain barrier (BBB). A brief description is provided of the tight junctions, which couple adjacent cerebral capillary endothelial cells to each other to form the barrier. Ammonia modulates the transcellular passage of low-to medium-size molecules, by affecting their carriers located at the BBB. Ammonia induces interrelated aberrations of the transport of the large neutral amino acids and aromatic amino acids (AAA), whose influx is augmented by exchange with glutamine produced in the course of ammonia detoxification, and maybe also modulated by the extracellularly acting gamma-glutamyl moiety transferring enzyme, gamma-glutamyl-transpeptidase. Impaired AAA transport affects neurotransmission by altering intracerebral synthesis of catecholamines (serotonin and dopamine), and producing “false neurotransmitters” (octopamine and phenylethylamine). Ammonia also modulates BBB transport of the cationic amino acids: the nitric oxide precursor, arginine, and ornithine, which is an ammonia trap, and affects the transport of energy metabolites glucose and creatine. Moreover, ammonia acting either directly or in synergy with liver injury-derived inflammatory cytokines also evokes subtle increases of the transcellular passage of molecules of different size (BBB “leakage”), which appears to be responsible for the vasogenic component of cerebral edema associated with ALF
Molecular targets of alcohol action: translational research for pharmacotherapy development and screening.
Alcohol abuse and dependence are multifaceted disorders with neurobiological, psychological, and environmental components. Research on other complex neuropsychiatric diseases suggests that genetically influenced intermediate characteristics affect the risk for heavy alcohol consumption and its consequences. Diverse therapeutic interventions can be developed through identification of reliable biomarkers for this disorder and new pharmacological targets for its treatment. Advances in the fields of genomics and proteomics offer a number of possible targets for the development of new therapeutic approaches. This brain-focused review highlights studies identifying neurobiological systems associated with these targets and possible pharmacotherapies, summarizing evidence from clinically relevant animal and human studies, as well as sketching improvements and challenges facing the fields of proteomics and genomics. Concluding thoughts on using results from these profiling technologies for medication development are also presented
Perispinal Etanercept for Post-Stroke Neurological and Cognitive Dysfunction: Scientific Rationale and Current Evidence
P: 69 Sarcopenia Pre- and Post-liver Transplantation: Implication for Hepatic Encephalopathy
The nutritional management of hepatic encephalopathy in patients with cirrhosis: Ishen consensus.
Mitochondrial Vulnerability and Increased Susceptibility to Nutrient-Induced Cytotoxicity in Fibroblasts from Leigh Syndrome French Canadian Patients
<div><p>Mutations in LRPPRC are responsible for the French Canadian variant of Leigh Syndrome (LSFC), a severe disorder characterized biochemically by a tissue-specific deficiency of cytochrome c oxidase (COX) and clinically by the occurrence of severe and deadly acidotic crises. Factors that precipitate these crises remain unclear. To better understand the physiopathology and identify potential treatments, we performed a comprehensive analysis of mitochondrial function in LSFC and control fibroblasts. Furthermore, we have used this cell-based model to screen for conditions that promote premature cell death in LSFC cells and test the protective effect of ten interventions targeting well-defined aspects of mitochondrial function. We show that, despite maintaining normal ATP levels, LSFC fibroblasts present several mitochondrial functional abnormalities under normal baseline conditions, which likely impair their capacity to respond to stress. This includes mitochondrial network fragmentation, impaired oxidative phosphorylation capacity, lower membrane potential, increased sensitivity to Ca<sup>2+</sup>-induced permeability transition, but no changes in reactive oxygen species production. We also show that LSFC fibroblasts display enhanced susceptibility to cell death when exposed to palmitate, an effect that is potentiated by high lactate, while high glucose or acidosis alone or in combination were neutral. Furthermore, we demonstrate that compounds that are known to promote flux through the electron transport chain independent of phosphorylation (methylene blue, dinitrophenol), or modulate fatty acid (L-carnitine) or Krebs cycle metabolism (propionate) are protective, while antioxidants (idebenone, N-acetyl cysteine, resveratrol) exacerbate palmitate plus lactate-induced cell death. Collectively, beyond highlighting multiple alterations in mitochondrial function and increased susceptibility to nutrient-induced cytotoxicity in LSFC fibroblasts, these results raise questions about the nature of the diets, particularly excess fat intake, as well as on the use of antioxidants in patients with LSFC and, possibly, other COX defects.</p></div
P: 43 A Sensitive and Convenient Protocol for Determining Brain Water Content in Rats using a Moisture Analyzer
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