The Political Career of Landon Carter Haynes

Summary: During the ante-bellum period, Landon Carter Haynes was closely associated with the history of Tennessee. As a member of the General Assembly from 1845 to 1850, he proposed and supported legislation regarding internal improvements, humanitarianism, education, reduction of state expenditures and indebtedness, and the location of a branch of the Bank of Tennessee in each of the three divisions of the state. One of Haynes\u27 chief objectives both as a public servant and a private citizen was the establishment of adequate railway transportation. As a member of the state legislature, he fought for state aid for railroads. Even during his political retirement, he continued to promote railroads by public addresses. These railroads were important transportation facilities during the War Between the States because of the lack of available water transportation. Politically, Haynes was a pro-southern Democrat who believed that Tennessee should co-operate with the South even if war were inevitable. His stand on states\u27 rights earned him a seat in the Confederate Senate during the war. Besides participating in the activities of that legislative body, he worked to improve the military situation of Tennessee. Regardless of his political achievements, Haynes\u27 fame as an orator was deeply embedded in the next generation. Today, most Tennesseans have forgotten his political attainments but they remember him as an orator

A GIS-Based Approach for Determining Potential Runoff Coefficient and Runoff Depth for the Indian River Lagoon, Florida, USA

The Indian River Lagoon system (IRL), spanning ~40% of Florida’s east coast, is one of the nation’s biggest and most biodiverse estuaries. In 2011, a super algal bloom event occurred in the IRL with total nitrogen and phosphorus levels that exceeded historical levels. Scientists suspect that nonpoint source pollution through surface runoff may have had a significant impact on the recent recurring algal blooms. Digital Elevation Model, land cover/land use, and soil data were used to calculate a runoff coefficient for the IRL drainage basin. Rainfall data were used to calculate runoff depth for the study area between the years of 2006–2016. When the monthly runoff depth data for 2011 were compared to a previous study on the 2011 super algal bloom in the lagoon, areas with high runoff visually matched the areas with higher chlorophyll a concentrations. Land development was a significant variable for determining runoff depth (p < 0.0001), and although used to derive runoff depths, the influence of precipitation was marginally significant (p = 0.06). Significant spatial autocorrelation indicated local trends between land development and runoff depth (p < 0.0001). Outputs will aid with decisions on stormwater management to more sustainable land development planning

Towards a conceptual framework demonstrating the effectiveness of audiovisual patient descriptions (patient video cases): a review of the current literature

Background: Technological advances have enabled the widespread use of video cases via web-streaming and online download as an educational medium. The use of real subjects to demonstrate acute pathology should aid the education of health care professionals. However, the methodology by which this effect may be tested is not clear. Methods: We undertook a literature review of major databases, found relevant articles relevant to using patient video cases as educational interventions, extracted the methodologies used and assessed these methods for internal and construct validity. Results: A review of 2532 abstracts revealed 23 studies meeting the inclusion criteria and a final review of 18 of relevance. Medical students were the most commonly studied group (10 articles) with a spread of learner satisfaction, knowledge and behaviour tested. Only two of the studies fulfilled defined criteria on achieving internal and construct validity. The heterogeneity of articles meant it was not possible to perform any meta-analysis. Conclusions: Previous studies have not well classified which facet of training or educational outcome the study is aiming to explore and had poor internal and construct validity. Future research should aim to validate a particular outcome measure, preferably by reproducing previous work rather than adopting new methods. In particular cognitive processing enhancement, demonstrated in a number of the medical student studies, should be tested at a postgraduate level

Dynamics and consequences of DNA looping by the FokI restriction endonuclease

Genetic events often require proteins to be activated by interacting with two DNA sites, trapping the intervening DNA in a loop. While much is known about looping equilibria, only a few studies have examined DNA-looping dynamics experimentally. The restriction enzymes that cut DNA after interacting with two recognition sites, such as FokI, can be used to exemplify looping reactions. The reaction pathway for FokI on a supercoiled DNA with two sites was dissected by fast kinetics to reveal, in turn: the initial binding of a protein monomer to each site; the protein–protein association to form the dimer, trapping the loop; the subsequent phosphodiester hydrolysis step. The DNA motion that juxtaposes the sites ought on the basis of Brownian dynamics to take ∼2 ms, but loop capture by FokI took 230 ms. Hence, DNA looping by FokI is rate limited by protein association rather than DNA dynamics. The FokI endonuclease also illustrated activation by looping: it cut looped DNA 400 times faster than unlooped DNA

Linking Nutrient Stoichiometry to Zika Virus Transmission in a Mosquito

Food quality and quantity serve as the basis for cycling of key chemical elements in trophic interactions; yet the role of nutrient stoichiometry in shaping host–pathogen interactions is under appreciated. Most of the emergent mosquito-borne viruses affecting human health are transmitted by mosquitoes that inhabit container systems during their immature stages, where allochthonous input of detritus serves as the basal nutrients. Quantity and type of detritus (animal and plant) were manipulated in microcosms containing newly hatched Aedes aegypti mosquito larvae. Adult mosquitoes derived from these microcosms were allowed to ingest Zika virus-infected blood and then tested for disseminated infection, transmission, and total nutrients (percent carbon, percent nitrogen, ratio of carbon to nitrogen). Treatments lacking high-quality animal (insect) detritus significantly delayed development. Survivorship to adulthood was closely associated with the amount of insect detritus present. Insect detritus was positively correlated with percent nitrogen, which affected Zika virus infection. Disseminated infection and transmission decreased with increasing insect detritus and percent nitrogen. We provide the first definitive evidence linking nutrient stoichiometry to arbovirus infection and transmission in a mosquito using a model system of invasive Ae. aegypti and emergent Zika virus

Regulation of Muscle Satellite Cell Activation and Chemotaxis by Angiotensin II

The role of angiotensin II (Ang II) in skeletal muscle is poorly understood. We report that pharmacological inhibition of Ang II signaling or ablation of the AT1a receptor significantly impaired skeletal muscle growth following myotrauma, in vivo, likely due to impaired satellite cell activation and chemotaxis. In vitro experiments demonstrated that Ang II treatment activated quiescent myoblasts as evidenced by the upregulation of myogenic regulatory factors, increased number of β-gal+, Myf5-LacZ myoblasts and the acquisition of cellular motility. Furthermore, exogenous treatment with Ang II significantly increased the chemotactic capacity of C2C12 and primary cells while AT1a−/− myoblasts demonstrated a severe impairment in basal migration and were not responsive to Ang II treatment. Additionally, Ang II interacted with myoblasts in a paracrine-mediated fashion as 4 h of cyclic mechanical stimulation resulted in Ang II-induced migration of cocultured myoblasts. Ang II-induced chemotaxis appeared to be regulated by multiple mechanisms including reorganization of the actin cytoskeleton and augmentation of MMP2 activity. Collectively, these results highlight a novel role for Ang II and ACE inhibitors in the regulation of skeletal muscle growth and satellite cell function

Say, What Shall Be My Song Tonight

[Verse 1] Say what shall my song be to night? And the strain at your bidding shall flow shall the measure be sportive and light, Or its murmurs be mournful and low? Shall the days that are gone, flit before thee? The freshness of childhood come o’er thee? Shall the past, yield it’s smiles and it’s tears? Or, the future, it’s hopes and it’s fears? [Chorus] Say, what shall my song be to night? And the stain at your bidding shall flow; Shall the measure be sportive and light? Or it’s murmurs be mournful and low? Say, say oh! Say, what shall my song be tonight? [Verse 2] There are times, when the heart will refuse, On the past and its pleasure to dwell; There are moments, which mem’ry imbues With a gloom which she cannot dispel: But the charm that enthralls them, is broken, With the first word of song that is spoken; For there is not a feeling, or tone, In the heart, but, to music ‘tis known. [Chorus

The 5.25 & 5.7 μ\mum Astronomical Polycyclic Aromatic Hydrocarbon Emission Features

Astronomical mid-IR spectra show two minor PAH features at 5.25 and 5.7 $\mu$m (1905 and 1754 cm$^{\rm - 1}$) that hitherto have been little studied, but contain information about the astronomical PAH population that complements that of the major emission bands. Here we report a study involving both laboratory and theoretical analysis of the fundamentals of PAH spectroscopy that produce features in this region and use these to analyze the astronomical spectra. The ISO SWS spectra of fifteen objects showing these PAH features were considered for this study, of which four have sufficient S/N between 5 and 6 $\mu$m to allow for an in-depth analysis. All four astronomical spectra show similar peak positions and profiles. The 5.25 $\mu$m feature is peaked and asymmetric, while the 5.7 $\mu$m feature is broader and flatter. Detailed analysis of the laboratory spectra and quantum chemical calculations show that the astronomical 5.25 and 5.7 $\mu$m bands are a blend of combination, difference and overtone bands primarily involving CH stretching and CH in-plane and CH out-of-plane bending fundamental vibrations. The experimental and computational spectra show that, of all the hydrogen adjacency classes possible on PAHs, solo and duo hydrogens consistently produce prominent bands at the observed positions whereas quartet hydrogens do not. In all, this a study supports the picture that astronomical PAHs are large with compact, regular structures. From the coupling with primarily strong CH out-of-plane bending modes one might surmise that the 5.25 and 5.7 $\mu$m bands track the neutral PAH population. However, theory suggests the role of charge in these astronomical bands might also be important.Comment: Accepted ApJ, 40 pages in pre-print, 14 figures, two onlin

Milling plant and soil material in plastic tubes over-estimates carbon and under-estimates nitrogen concentrations

Peer reviewedPostprin