Estudio de la regulación de los genes Six3 en vertebrados

Teis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular Bioquíca. Fecha de lectura:19-05-201

The Accretion Dicsc in Halfa with OmegaCAM (ADHOC) Survey

We present the first results of the Accretion Discs in Hα with OmegaCAM (ADHOC) survey, which aims to perform a deep and homogeneous photometric study of pre-main sequence (PMS) stars in a number of nearby star-forming regions. We took advantage of the exquisite image quality and wide-field capabilities of OmegaCAM at the VLT Survey Telescope (VST) to perform multi-band (ugri and Hα), deep (iSDSS < 22 mag), homogeneous and wide-field (covering tens of parsecs) observations of eight star-forming regions: the Orion Nebula Cluster, Lupus, Sco-Cen, Haffner 18, Vela OB2, Eta Cha, Chamaeleon and Ophiuchus. Using a robust method to identify PMS stars through their photometric excess in the Hα band, we aim to measure physical parameters (including mass accretion rates) for over 10 000 PMS stars. Direct comparison with low-resolution spectroscopy confirms that the objects with Hα excess emission that are detected photometrically are bona-fide PMS stars. The first results from this study clearly demonstrate the validity of the observational approach to unveiling complex stellar populations in young clusters.Fil: Beccari, Giacomo. European Southern Observatory Chile; ChileFil: Petr-Gotzens, Monika G.. European Southern Observatory Chile; ChileFil: Boffin, Henri M. J.. European Southern Observatory Chile; ChileFil: Jerabkova, Tereza. European Southern Observatory Chile; ChileFil: Romaniello, Martino. European Southern Observatory Chile; ChileFil: Areal, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Carraro, Giovanni. Dipartimento Di Fisica E Astronomia Galileo Galile; ItaliaFil: Celis Peña, Mariela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: De Marchi, Guido De. European Space Research And Technology Centre; Países BajosFil: de Wit, Willem-Jan. European Southern Observatory Chile; ChileFil: Drew, Janet E.. Centre For Astrophysics Research; Reino UnidoFil: Fedele, Davide. Osservatorio Astrofisico Di Arcetri; ItaliaFil: Ferrero, Leticia Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Observatorio Astronómico de Córdoba; ArgentinaFil: Kalari, Venu M.. Universidad de Santiago de Chile; ChileFil: Manara, Carlo F.. European Southern Observatory Chile; ChileFil: Mardones, Diego. Universidad de Santiago de Chile; ChileFil: Martin, Eduardo L.. Centro de Astrobiologia Madrid; EspañaFil: Meza, Erick. Université Pierre et Marie Curie; FranciaFil: Mieske, Steffen. Universidad de Santiago de Chile; ChileFil: Panagia, Nino. Space Telescope Science Institute; Estados UnidosFil: Testi, Leonardo. European Southern Observatory Chile; ChileFil: Vink, Jorick S.. Armagh Observatory; Reino UnidoFil: Walsh, Jeremy R.. European Southern Observatory Chile; ChileFil: Wright, Nick J.. Keele University.; Reino Unid

Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids.

The emergence of multiple axes is an essential element in the establishment of the mammalian body plan. This process takes place shortly after implantation of the embryo within the uterus and relies on the activity of gene regulatory networks that coordinate transcription in space and time. Whereas genetic approaches have revealed important aspects of these processes1, a mechanistic understanding is hampered by the poor experimental accessibility of early post-implantation stages. Here we show that small aggregates of mouse embryonic stem cells (ESCs), when stimulated to undergo gastrulation-like events and elongation in vitro, can organize a post-occipital pattern of neural, mesodermal and endodermal derivatives that mimic embryonic spatial and temporal gene expression. The establishment of the three major body axes in these 'gastruloids'2,3 suggests that the mechanisms involved are interdependent. Specifically, gastruloids display the hallmarks of axial gene regulatory systems as exemplified by the implementation of collinear Hox transcriptional patterns along an extending antero-posterior axis. These results reveal an unanticipated self-organizing capacity of aggregated ESCs and suggest that gastruloids could be used as a complementary system to study early developmental events in the mammalian embryo.This work was supported by funds from the BBSRC (No. BB/M023370/1 and BB/P003184/1 to A.M.A.), an NC3Rs David Sainsbury Fellowship (No. NC/P001467/1 to D.A.T.), an Engineering and Physical Sciences Research Council (EPSRC) Studentship (to P.B.-J.), a Company of Biologists Development Travelling Fellowship (DEVTF-151210 to P.B.-J.), a Newnham College Constance Work Junior Research Fellowship (to N.M.), the École Polytechnique Fédérale de Lausanne (D.D. and M.P.L.), the University of Geneva (D.D.), the Swiss National Research Fund (No. 310030B_138662 to D.D.) and the European Research Council grants ERC MOFDH (No. 250316 to A.M.A.), RegulHox (No 588029 to D.D.) and STEMCHIP (No 311422 to M.P.L.)

The Transcription Factor Encyclopedia

Here we present the Transcription Factor Encyclopedia (TFe), a new web-based compendium of mini review articles on transcription factors (TFs) that is founded on the principles of open access and collaboration. Our consortium of over 100 researchers has collectively contributed over 130 mini review articles on pertinent human, mouse and rat TFs. Notable features of the TFe website include a high-quality PDF generator and web API for programmatic data retrieval. TFe aims to rapidly educate scientists about the TFs they encounter through the delivery of succinct summaries written and vetted by experts in the field. TFe is available at http://www.cisreg.ca/tfe .Medical Genetics, Department ofMedicine, Faculty ofMolecular Medicine and Therapeutics, Centre forScience, Faculty ofNon UBCReviewedFacult