31 research outputs found

    It’s GIrls’ Day! What sketch maps show about girls’ spatial knowledge

    Get PDF
    Ponencias, comunicaciones y pósters presentados en el 17th AGILE Conference on Geographic Information Science "Connecting a Digital Europe through Location and Place", celebrado en la Universitat Jaume I del 3 al 6 de junio de 2014.This paper describes the analysis of sketch maps from girls who participated in the Girls’ Day annual event in Germany. The event caters to girls from Grades 7 – 10 as an opportunity to experience various jobs that might interest them in the future, typically within the STEM-disciplines. One of the performed activities was asking the girls who participated to draw a sketch map of an area they are familiar with. We are interested in finding out how girls externalize the environment they were told to draw. The activity also helps us understand how they organize their environmental knowledge through sketch maps. This descriptive work deviates from gender comparison of map-making by focusing only on girls. This paper allows us to understand differences of girls’ cognitive abilities based on what they have drawn on the map. The results showed that girls draw map ranging from egocentric pictorial representation with few details to survey structured map. More than 40% of the girls have included landmarks and streets outside the region of interest showing a more global view of the area. Landmarks frequently drawn showed visual, structural and cognitive characteristics. This study contributes to research related to better understanding of the cognitive abilities of young adults, particularly girls

    Desenvolvimento do Cadastron na Alemanha

    Get PDF
    The first part of the paper will give an overview on the classification of ALKISwithin the German NSDI initiative. The following two parts describe the ALKISModeland the implementation of the system in the case of North Rhine-Westphalia.The penultimate point focuses upon the discussion of an increasing use and value of(official) geographical data. In a final step, future prospects are presented.A primeira parte do artigo traz uma visão geral do sistema ALKIS - AmtlichesLiegenschaftskataster Informationssystem, que é uma iniciativa do GovernoAlemão dentro do NSDI - National Spatial Data Infrastructure. As duas partesseguintes descrevem o modelo ALKIS e a implantação do sistema em uma regiãopiloto, a de Rhine-Westphalia Norte. No penúltimo item é formulada uma discussãosobre a utilização e o crescente valor dos dados espaciais. Na etapa do final é feitauma apresentação sobre o futuro do sistema

    Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency

    Get PDF
    Cytosine DNA bases can be methylated by DNA methyltransferases and subsequently oxidized by TET proteins. The resulting 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) are considered demethylation intermediates as well as stable epigenetic marks. To dissect the contributions of these cytosine modifying enzymes, we generated combinations of Tet knockout (KO) embryonic stem cells (ESCs) and systematically measured protein and DNA modification levels at the transition from naive to primed pluripotency. Whereas the increase of genomic 5-methylcytosine (5mC) levels during exit from pluripotency correlated with an upregulation of the de novo DNA methyltransferases DNMT3A and DNMT3B, the subsequent oxidation steps turned out to be far more complex. The strong increase of oxidized cytosine bases (5hmC, 5fC, and 5caC) was accompanied by a drop in TET2 levels, yet the analysis of KO cells suggested that TET2 is responsible for most 5fC formation. The comparison of modified cytosine and enzyme levels in Tet KO cells revealed distinct and differentiation-dependent contributions of TET1 and TET2 to 5hmC and 5fC formation arguing against a processive mechanism of 5mC oxidation. The apparent independent steps of 5hmC and 5fC formation suggest yet to be identified mechanisms regulating TET activity that may constitute another layer of epigenetic regulation

    Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

    Get PDF
    The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form

    Assessing the Usability of WebGIS for Schools. GI_Forum 2013 – Creating the GISociety|

    No full text
    This work introduces concepts from usability engineering and user centered design into software development in the educational context. As still most digital geomedia on the educational market are based on classical GIS principles made for professionals there is a need for usable tools that fit the needs of students and teachers. A prototype WebGIS application for schools to present various geospatial datasets with an intuitive user interface will be presented. The used technologies and GUI elements are described. Usability tests with secondary school students are conducted and are used to assess the performance of the prototype web application in a realistic environment
    corecore