Participação das famílias a través de comunidades de aprendizagem

In current education, it is essential to articulate inclusion in training processes and thus promote the participation of all those involved (school, family, State, and society in general), therefore, the general objective of the research was to strengthen the participation of the family in the educational processes of the students, through learning communities at the El Corozo headquarters of the Patio Bonito Educational Institution in Montería - Córdoba. The methodology was based on a qualitative approach, under an action research design, to establish the role that the family must assume from the learning communities in favor of their participation and inclusion in the processes that children advance in school. The population was constituted by the educational community already mentioned, the sample was the 21 parents of the 26 boys and girls between 8 and 13 years old, who are enrolled and belong to the 3rd, 4th, and 5th grades of the El Corozo campus. The instruments used were two interviews, one with parents and the other with the guidance teacher, participant observation and a group session; the analysis categories constructed were participation, learning communities and communication; Among the findings, the existence of intuitive learning communities without the direct and formal influence of the educational processes advanced by the school was found, highlighting the need to create support networks that open spaces for family participation in all levels of the institution.En la educación actual, es indispensable articular la inclusión a los procesos formativos y, así, promover la participación de todos los involucrados (escuela, familia, Estado y sociedad en general), por tanto, el objetivo general de la investigación fue fortalecer la participación de la familia en los procesos educativos de los estudiantes, a través, de comunidades de aprendizaje en la sede El Corozo de la Institución Educativa Patio Bonito de Montería, Córdoba. La metodología se basó en un enfoque cualitativo, bajo un diseño de investigación acción, para establecer el rol que la familia debe asumir desde las comunidades de aprendizaje en pro de su participación e inclusión a los procesos que adelantan los niños en la escuela. La población, estuvo constituida por la comunidad educativa ya mencionada, la muestra fueron los 21 padres de familia de los 26 niños y niñas entre los 8 y 13 años; que se encuentran matriculados y pertenecen a los grados 3º, 4º y 5º de la sede El Corozo. Los instrumentos utilizados fueron dos entrevistas, una a padres y otra al docente orientador, observación participante y una sesión de grupo; las categorías de análisis construidas fueron participación, comunidades de aprendizaje y comunicación; entre los hallazgos, se encontró, la existencia de comunidades de aprendizaje intuitivas sin la influencia directa y formal de los procesos educativos adelantados por la escuela, destacándose la necesidad de crear redes de apoyo que abran espacios a la participación familiar en todos los estamentos de la institución.Na educação atual é fundamental articular a inclusão nos processos de formação e assim promover a participação de todos os envolvidos (escola, família, Estado e sociedade em geral), portanto, o objetivo geral da pesquisa foi fortalecer a participação da família nos processos educativos dos alunos, através de comunidades de aprendizagem na sede El Corozo da Instituição Educacional Patio Bonito de Montería - Córdoba. A metodologia foi baseada em uma abordagem qualitativa, sob um desenho de pesquisa-ação, para estabelecer o papel que a família deve assumir a partir das comunidades de aprendizagem em favor de sua participação e inclusão nos processos que as crianças avançam na escola. A população foi constituída pela comunidade educativa já referida, a amostra foram os 21 pais dos 26 rapazes e raparigas entre os 8 e os 13 anos; que estejam matriculados e pertençam à 3ª, 4ª e 5ª séries do campus El Corozo. Os instrumentos utilizados foram duas entrevistas, uma com os pais e outra com a orientadora, observação participante e uma sessão de grupo; as categorias de análise construídas foram participação, comunidades de aprendizagem e comunicação; Entre os achados, encontrou-se a existência de comunidades intuitivas de aprendizagem sem a influência direta e formal dos processos educacionais promovidos pela escola, evidenciando a necessidade de criação de redes de apoio que abram espaços para a participação da família em todos os níveis da instituição

Inhibition pattern of sulfamide-related compounds in binding to carbonic anhydrase isoforms I, II, VII, XII and XIV

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Evaluation of a lyophilized CRISPR-Cas12 assay for a sensitive, specific, and rapid detection of SARS-CoV-2

We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the Lyo-CRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the two diagnostic tests. An initial validation test was also performed on 30 nasopharyngeal samples collected in lysis buffer, in which the Lyo-CRISPR SARS-CoV-2 kit detected 20/21 (95.24%; 95% CI: 76.18–99.88) positive samples and 9/9 (100%; 95% CI: 66.37–100) negative samples. The estimated Kappa index was 0.923, indicating a strong concordance between the test procedures. The Lyo-CRISPR SARS-CoV-2 kit was suitable for detecting a wide range of RT-qPCR-positive samples (cycle threshold range: 11.45–36.90) and dilutions of heat-inactivated virus (range: 2.5–100 copies/µL); no cross-reaction was observed with the other respiratory pathogens tested. We demonstrated that the performance of the Lyo-CRISPR SARS-CoV-2 kit was similar to that of commercial RT-qPCR, as the former was highly sensitive and specific, timesaving (1.5 h), inexpensive, and did not require sophisticated equipment. The use of this kit would reduce the time taken for diagnosis and facilitate molecular diagnosis in low-resource laboratories.Instituto de VirologíaFil: Curti, Lucía Ana. CASPR Biotech; Estados UnidosFil: Primost, Ivana. Hospital Municipal de Trauma y Emergencias Dr. Federico Abete. Genetics and Molecular Biology Laboratory; ArgentinaFil: Valla, Sofia. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA). Centro de Investigaciones Básicas y Aplicadas (CIBA); ArgentinaFil: Valla, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ibañez Alegre, Daiana. Universidad Nacional de Misiones. Instituto de Biología Subtropical. Laboratorio Grupo de Investigación en Genética Aplicada (GIGA); ArgentinaFil: Ibañez Alegre, Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olguin Perglione, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Olguin Perglione, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Repizo, Guillermo Daniel. CASPR Biotech; Estados UnidosFil: Lara, Julia. CASPR Biotech; Estados UnidosFil: Parcerisa, Ivana. CASPR Biotech; Estados UnidosFil: Palacios, Antonela. CASPR Biotech; Estados UnidosFil: Llases, María Eugenia. CASPR Biotech; Estados UnidosFil: Rinflerch, Adriana. Universidad Nacional de Misiones. Instituto de Biología Subtropical. Laboratorio Grupo de Investigación en Genética Aplicada (GIGA); ArgentinaFil: Rinflerch, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrios, Melanie. Universidad de Buenos Aires. Instituto de Producción Agropecuaria; ArgentinaFil: Pereyra Bonnet, Federico. CASPR Biotech; Estados UnidosFil: Gimenez, Carla Alejandra. CASPR Biotech; Estados UnidosFil: Marcone, Débora Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología, Biotecnología y Genética. Cátedra de Virología; ArgentinaFil: Marcone, Débora Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model

Background: Cross-reactivity between soybean allergens and bovine caseins has been previously reported. In this study we aimed to map epitopes of the major soybean allergen Gly m 5 that are co-recognized by casein specific antibodies, and to identify a peptide responsible for the cross-reactivity. Methods: Cow's milk protein (CMP)-specific antibodies were used in different immunoassays (immunoblotting, ELISA, ELISA inhibition test) to evaluate the in vitro recognition of soybean proteins (SP). Recombinant Gly m 5 (α), a truncated fragment containing the C-terminal domain (α-T) and peptides of α-T were obtained and epitope mapping was performed with an overlapping peptide assay. Bioinformatics tools were used for epitope prediction by sequence alignment, and for modelling the cross-recognized soy proteins and peptides. The binding of SP to a monoclonal antibody was studied by surface Plasmon resonance (SPR). Finally, the in vivo cross-recognition of SP was assessed in a mouse model of milk allergy. Results: Both α and α-T reacted with the different CMP-specific antibodies. α-T contains IgG and IgE epitopes in several peptides, particularly in the peptide named PA. Besides, we found similar values of association and dissociation constants between the α-casein specific mAb and the different milk and soy components. The food allergy mouse model showed that SP and PA contain the cross-reactive B and T epitopes, which triggered hypersensitivity reactions and a Th2-mediated response on CMP-sensitized mice. Conclusions: Gly m 5 is a cross-reactive soy allergen and the α-T portion of the molecule contains IgG and IgE immunodominant epitopes, confined to PA, a region with enough conformation to be bound by antibodies. These findings contribute to explain the intolerance to SP observed in IgE-mediated CMA patients, primarily not sensitised to SP, as well as it sets the basis to propose a mucosal immunotherapy for milk allergy using this soy peptide.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesCentro de Investigación y Desarrollo en Criotecnología de AlimentosLaboratorio de Investigaciones del Sistema InmuneFacultad de Ciencias Exacta

Inhibition pattern of sulfamide-related compounds in binding to carbonic anhydrase isoforms I, II, VII, XII and XIV

A set of sulfamides and sulfamates were synthesized and tested against several isoforms of carbonic anhydrase: CA I, CA II, CA VII, CA XII and CA XIV. The biological assays showed a broad range of inhibitory activity, and interesting results were found for several compounds in terms of activity (Ki <1μm) and selectivity: some aromatic sulfamides are active against CA I, CA II and/or CA VII; while they are less active in CA XII and CA XIV. On the other hand, bulky sulfamides are selective to CA VII. To understand the origin of the different inhibitory activity against each isozyme we used molecular modeling techniques such as docking and molecular dynamic simulations.Facultad de Ciencias Exacta

Targeting a cross-reactive Gly m 5 soy peptide as responsible for hypersensitivity reactions in a milk allergy mouse model

Background: Cross-reactivity between soybean allergens and bovine caseins has been previously reported. In this study we aimed to map epitopes of the major soybean allergen Gly m 5 that are co-recognized by casein specific antibodies, and to identify a peptide responsible for the cross-reactivity. Methods: Cow's milk protein (CMP)-specific antibodies were used in different immunoassays (immunoblotting, ELISA, ELISA inhibition test) to evaluate the in vitro recognition of soybean proteins (SP). Recombinant Gly m 5 (α), a truncated fragment containing the C-terminal domain (α-T) and peptides of α-T were obtained and epitope mapping was performed with an overlapping peptide assay. Bioinformatics tools were used for epitope prediction by sequence alignment, and for modelling the cross-recognized soy proteins and peptides. The binding of SP to a monoclonal antibody was studied by surface Plasmon resonance (SPR). Finally, the in vivo cross-recognition of SP was assessed in a mouse model of milk allergy. Results: Both α and α-T reacted with the different CMP-specific antibodies. α-T contains IgG and IgE epitopes in several peptides, particularly in the peptide named PA. Besides, we found similar values of association and dissociation constants between the α-casein specific mAb and the different milk and soy components. The food allergy mouse model showed that SP and PA contain the cross-reactive B and T epitopes, which triggered hypersensitivity reactions and a Th2-mediated response on CMP-sensitized mice. Conclusions: Gly m 5 is a cross-reactive soy allergen and the α-T portion of the molecule contains IgG and IgE immunodominant epitopes, confined to PA, a region with enough conformation to be bound by antibodies. These findings contribute to explain the intolerance to SP observed in IgE-mediated CMA patients, primarily not sensitised to SP, as well as it sets the basis to propose a mucosal immunotherapy for milk allergy using this soy peptide.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesCentro de Investigación y Desarrollo en Criotecnología de AlimentosLaboratorio de Investigaciones del Sistema InmuneFacultad de Ciencias Exacta

Using a Taxonomy to Systematically Identify and Describe Self-Management Interventions Components in Randomized Trials for COPD

Self-management interventions (SMIs) may improve outcomes in Chronic Obstructive Pulmonary Disease (COPD). However, accurate comparisons of their relative effectiveness are challenging, partly due to a lack of clarity and detail regarding the intervention content being evaluated. This study systematically describes intervention components and characteristics in randomized controlled trials (RCTs) related to COPD self-management using the COMPAR-EU taxonomy as a framework, identifying components that are insufficiently incorporated into the design of the intervention or insufficiently reported. Overall, 235 RCTs published between 2010 and 2018, from a systematic review were coded using the taxonomy, which includes 132 components across four domains: intervention characteristics, expected patient (or caregiver) self-management behaviours, patient relevant outcomes, and target population characteristics. Risk of bias was also assessed. Interventions mainly focused on physical activity (67.4%), and condition-specific behaviours like breathing exercise (63.5%), self-monitoring (50.8%), and medication use (33.9%). Support techniques like education and skills-training, self-monitoring, and goal setting (over 35% of the RCTs) were mostly used for this. Emotional-based techniques, problem-solving, and shared decision-making were less frequently reported (less than 15% of the studies). Numerous SMIs components were insufficiently incorporated into the design of COPD SMIs or insufficiently reported. Characteristics like mode of delivery, intensity, location, and providers involved were often not described. Only 8% of the interventions were tailored to the target population's characteristics. Outcomes that are considered important by patients were hardly taken into account. There is still a lot to improve in both the design and description of SMIs for COPD. Using a framework such as the COMPAR-EU SMI taxonomy may contribute to better reporting and to better informing of replication efforts. In addition, prospective use of the taxonomy for developing and reporting intervention content would further aid in building a cumulative science of effective SMIs in COPD

Polygenic Risk Scores for Prediction of Breast Cancer and Breast Cancer Subtypes

Stratification of women according to their risk of breast cancer based on polygenic risk scores (PRSs) could improve screening and prevention strategies. Our aim was to develop PRSs, optimized for prediction of estrogen receptor (ER)-specific disease, from the largest available genome-wide association dataset and to empirically validate the PRSs in prospective studies. The development dataset comprised 94,075 case subjects and 75,017 control subjects of European ancestry from 69 studies, divided into training and validation sets. Samples were genotyped using genome-wide arrays, and single-nucleotide polymorphisms (SNPs) were selected by stepwise regression or lasso penalized regression. The best performing PRSs were validated in an independent test set comprising 11,428 case subjects and 18,323 control subjects from 10 prospective studies and 190,040 women from UK Biobank (3,215 incident breast cancers). For the best PRSs (313 SNPs), the odds ratio for overall disease per 1 standard deviation in ten prospective studies was 1.61 (95%CI: 1.57-1.65) with area under receiver-operator curve (AUC) = 0.630 (95%CI: 0.628-0.651). The lifetime risk of overall breast cancer in the top centile of the PRSs was 32.6%. Compared with women in the middle quintile, those in the highest 1% of risk had 4.37- and 2.78-fold risks, and those in the lowest 1% of risk had 0.16- and 0.27-fold risks, of developing ER-positive and ER-negative disease, respectively. Goodness-of-fit tests indicated that this PRS was well calibrated and predicts disease risk accurately in the tails of the distribution. This PRS is a powerful and reliable predictor of breast cancer risk that may improve breast cancer prevention programs.NovartisEli Lilly and CompanyAstraZenecaAbbViePfizer UKCelgeneEisaiGenentechMerck Sharp and DohmeRocheCancer Research UKGovernment of CanadaArray BioPharmaGenome CanadaNational Institutes of HealthEuropean CommissionMinistère de l'Économie, de l’Innovation et des Exportations du QuébecSeventh Framework ProgrammeCanadian Institutes of Health Researc

Sulfamatos y sulfamidas alifáticas: su acción anticonvulsiva : Diseño, síntesis y actividad farmacológica

Facultad de Ciencias Exacta

Site selective 3d heterometallic molecular strings based on bis-β-diketone ligand

Resumen del trabajo presentado a la: "14th International Conference on Molecule-Based Magnets" celebrada en San Petersburgo (Rusia) del 5 al 9 de julio de 2014.One of the major challenges of coordination chemistry today is the design and synthesis of molecules with predetermined functions and physicochemical properties. Our research group is addressing this challenge through the preparation of polydentate ligands from the bis-β-diketone family, capable of gathering transition metals within molecular clusters of well-defined nuclearity and topology. Known structural motifs arising from these platforms are weakly coupled cluster pairs as prototypes of 2-qubit quantum gates, and chain-like arrays as potential prototypes of molecular wires. One of the ligands capable to accommodate metal ions into linear chains is trispyridyl/ bis-β-diketone, H2L, (1,3-bis-(3-oxo-3-(2-pyridyl)propionyl)pyridine) as demonstrated with the synthesis of the supramolecular array [Co4L2(MeOH)8](NO3)4 and its analogues. We are now presenting the potential of this ligand for the preparation of new 3d heterometallic molecular strings with site selective metal composition as a direct consequence of crystal field energy effects. Among them, the most interesting cases are tetranuclear and octanuclear Co-Ni systems where two very similar metals are easily differentiated due to the above described selectivity. From the synthetic point of view, it will be shown how small changes in reaction conditions can vary the nature of the obtained system leading from charged species to neutral molecules. The solid state and solution behaviour of those systems will be discussed following crystallographic, spectroscopic, magnetic and redox studies.We are grateful to the ERC for a financial support under Starting Grant 258060 FuncMolQIP.Peer reviewe