Anti-oxidant effect of gold nanoparticles restrains hyperglycemic conditions in diabetic mice

<p>Abstract</p> <p>Background</p> <p>Oxidative stress is imperative for its morbidity towards diabetic complications, where abnormal metabolic milieu as a result of hyperglycemia, leads to the onset of several complications. A biological antioxidant capable of inhibiting oxidative stress mediated diabetic progressions; during hyperglycemia is still the need of the era. The current study was performed to study the effect of biologically synthesized gold nanoparticles (AuNPs) to control the hyperglycemic conditions in streptozotocin induced diabetic mice.</p> <p>Results</p> <p>The profound control of AuNPs over the anti oxidant enzymes such as GSH, SOD, Catalase and GPx in diabetic mice to normal, by inhibition of lipid peroxidation and ROS generation during hyperglycemia evidence their anti-oxidant effect during hyperglycemia. The AuNPs exhibited an insistent control over the blood glucose level, lipids and serum biochemical profiles in diabetic mice near to the control mice provokes their effective role in controlling and increasing the organ functions for better utilization of blood glucose. Histopathological and hematological studies revealed the non-toxic and protective effect of the gold nanoparticles over the vital organs when administered at dosage of 2.5 mg/kilogram.body.weight/day. ICP-MS analysis revealed the biodistribution of gold nanoparticles in the vital organs showing accumulation of AuNPs in the spleen comparatively greater than other organs.</p> <p>Conclusion</p> <p>The results obtained disclose the effectual role of AuNPs as an anti-oxidative agent, by inhibiting the formation of ROS, scavenging free radicals; thus increasing the anti-oxidant defense enzymes and creating a sustained control over hyperglycemic conditions which consequently evoke the potential of AuNPs as an economic therapeutic remedy in diabetic treatments and its complications.</p

Gold nanoparticles supported on nanoparticulate ceria as a powerful agent against intracellular oxidative stress

Ceria-supported gold nanoparticles are prepared exhibiting peroxidase activity and acting as radical traps. Au/CeO2 shows a remarkable biocompatibility as demonstrated by measuring cellular viability, proliferation, and lack of apoptosis for two human cell lines (Hep3B and HeLa). The antioxidant activity of Au/CeO2 against reactive oxygen species (ROS) is demonstrated by studying the cellular behavior of Hep3B and HeLa in a model of cellular oxidative stress. It is determined that Au/CeO2 exhibits higher antioxidant activity than glutathione, the main cytosolic antioxidant compound, and its CeO2 carrier. Overall the result presented here shows the potential of implementing well-established nanoparticulated gold catalysts with remarkable biocompatibility in cellular biology.Financial support by the Spanish ministry of Science is (CTQ2006-06785 and CTQ2007-67805-AR07, PI10/1195, AP192/11) is gratefully acknowledged. RM also thanks Spanish Ministry of Education for a postgraduate scholarship. VMV is recipient of Regional Ministry of Health of the Valencian Community and Carlos III Health Institute contract (ACOMP/2012/045 and CES10/030). NA is a recipient of VALi+D contract (APOSTD/2011/049).Menchón, C.; Martín González, R.; Apostolova, N.; Victor, VM.; Alvaro Rodríguez, MM.; Herance Camacho, JR.; García Gómez, H. (2012). Gold nanoparticles supported on nanoparticulate ceria as a powerful agent against intracellular oxidative stress. Small. 8(12):1895-1903. https://doi.org/10.1002/smll.201102255S1895190381