Development and pilot evaluation of a novel probiotic mixture for the management of seasonal allergic rhinitis

The effect of heat treatment at different temperatures on two types of inocula, activated sludge and anaerobically digested sludge, was investigated in batch cultures. Heat treatments were conducted at 65, 80 and 95 °C for 30 min. The untreated inocula produced less amount of hydrogen than the pretreated inocula, with lactic acid as the main metabolite. The maximum yields of 2.3 and 1.6 mol H /mol glucose were achieved for the 65 °C pretreated anaerobically digested and activated sludges, respectively. Approximately a 15% decrease in yield was observed with increasing pretreatment temperature from 65 to 95 °C concomitant with an increase in butyrate/acetate ratio from 1.5 to 2.4 for anaerobically digested sludge. The increase of pretreatment temperature of activated sludge to 95 °C suppressed the hydrogen production by lactic acid fermentation. DNA analysis of the microbial community showed that the elevated pretreatment temperatures reduced the species diversity. © 2008 International Association for Hydrogen Energy.

Comparative study of biological hydrogen production by pure strains and consortia of facultative and strict anaerobic bacteria

In this paper, a simple and rapid method was developed in order to assess in comparative tests the production of binary biogas mixtures containing CO2 and another gaseous compound such as hydrogen or methane. This method was validated and experimented for the characterisation of the biochemical hydrogen potential of different pure strains and mixed cultures of hydrogen-producing bacteria (HPB) growing on glucose. The experimental results compared the hydrogen production yield of 19 different pure strains and sludges : facultative and strict anaerobic HPB strains along with anaerobic digester sludges thermally pre-treated or not. Significant yields variations were recorded even between different strains of the same species by i.e. about 20% for three Clostridium butyricum strains. The pure Clostridium butyricum and pasteurianum strains achieved the highest yields i.e. up to 1,36 mol H2/mol glucose compared to the yields achieved by the sludges and the tested Escherichia and Citrobacter strains.Etude de la production d'hydrogène par les bactéries anaérobies chimiotrophes (dark-fermentation

Simple Enrichment System for Hydrogen Producers ▿

This study presents a simple enrichment system where gas pressure produced by microbes performs functions that are normally done by labor. The system was tested with Escherichia coli strains with different hydrogen production and growth capabilities. The results show that the system can enrich the best hydrogen producer