Influencing user behavior in office buildings through a co-creation process in order to achieve better energy efficiency and comfort

Providing users with relevant information raises their awareness on the influence of their behavior on the energy consumption and comfort in their workspace. A user-building interface was developed as part of this research through a co-creation process. It provides the occupants of monitored offices with information on their electricity and heating consumption, comfort indicators (temperature and air quality), light control and hints and tips on how to improve their behavior. This contribution describes the methodology used to improve the behavior of users in a pilot building and the results of a 32-week measurement campaign

Improved primordial non-gaussianity constraints from measurements of galaxy clustering and the integrated Sachs-Wolfe effect

We present the strongest robust constraints on primordial non-Gaussianity (PNG) from currently available galaxy surveys, combining large-scale clustering measurements and their cross-correlations with the cosmic microwave background. We update the data sets used by Giannantonio et al. (2012), and broaden that analysis to include the full set of two-point correlation functions between all surveys. In order to obtain the most reliable constraints on PNG, we advocate the use of the cross-correlations between the catalogs as a robust estimator and we perform an extended analysis of the possible systematics to reduce their impact on the results. To minimize the impact of stellar contamination in our luminous red galaxy (LRG) sample, we use the recent Baryon Oscillations Spectroscopic Survey catalog of Ross et al. (2011). We also find evidence for a new systematic in the NVSS radio galaxy survey similar to, but smaller than, the known declination-dependent issue; this is difficult to remove without affecting the inferred PNG signal, and thus we do not include the NVSS auto-correlation function in our analyses. We find no evidence of primordial non-Gaussianity; for the local-type configuration we obtain for the skewness parameter $-36 < f_{\mathrm{NL}} < 45$ at 95 % c.l. ($5 \pm 21$ at $1\sigma$) when using the most conservative part of our data set, improving previous results; we also find no evidence for significant kurtosis, parameterized by $g_{\mathrm{NL}}$. In addition to PNG, we simultaneously constrain dark energy and find that it is required with a form consistent with a cosmological constant.Comment: 24 pages, 16 figures. More conservative treatment of the NVSS data, version accepted by Phys. Rev.

Species richness-environment relationships of European arthropods at two spatial grains : habitats and countries

We study how species richness of arthropods relates to theories concerning net primary productivity, ambient energy, water-energy dynamics and spatial environmental heterogeneity. We use two datasets of arthropod richness with similar spatial extents (Scandinavia to Mediterranean), but contrasting spatial grain (local habitat and country). Samples of ground-dwelling spiders, beetles, bugs and ants were collected from 32 paired habitats at 16 locations across Europe. Species richness of these taxonomic groups was also determined for 25 European countries based on the Fauna Europaea database. We tested effects of net primary productivity (NPP), annual mean temperature (T), annual rainfall (R) and potential evapotranspiration of the coldest month (PETmin) on species richness and turnover. Spatial environmental heterogeneity within countries was considered by including the ranges of NPP, T, R and PETmin. At the local habitat grain, relationships between species richness and environmental variables differed strongly between taxa and trophic groups. However, species turnover across locations was strongly correlated with differences in T. At the country grain, species richness was significantly correlated with environmental variables from all four theories. In particular, species richness within countries increased strongly with spatial heterogeneity in T. The importance of spatial heterogeneity in T for both species turnover across locations and for species richness within countries suggests that the temperature niche is an important determinant of arthropod diversity. We suggest that, unless climatic heterogeneity is constant across sampling units, coarse-grained studies should always account for environmental heterogeneity as a predictor of arthropod species richness, just as studies with variable area of sampling units routinely consider area

Metodo per stabilire una corrispondenza a posteriori tra un pezzo di legno ed un tronco da cui il pezzo di legno era stato ottenuto

Metodo per stabilire una corrispondenza a posteriori tra un pezzo di legno ed un tronco da cui pezzo di legno sia stato ottenuto, comprendente le seguenti fasi operative: eseguire una tomografia del tronco di legno, calcolare o selezionare uno schema di taglio del tronco, determinare una o più caratteristiche individualizzanti virtuali legate alla distribuzione e/o dimensione di caratteristiche fisiche del tronco all'interno e/o sulla superficie del pezzo di legno virtuale stesso a partire dalle informazioni tomografiche disponibili, memorizzarle in una banca dati, in abbinamento ad informazioni circa l'identità del tronco, suddividere il tronco in pezzi di legno reali secondo lo schema di taglio, acquisire informazioni reali circa la distribuzione e/o la dimensione di caratteristiche fisiche del tronco all'interno e/o sulla superficie di un pezzo di legno reale e determinare corrispondenti caratteristiche individualizzanti reali da confrontare con caratteristiche individualizzanti virtuali memorizzate e identificare un'origine del pezzo di legno reale sulla base delle informazioni circa l'identità del tronco memorizzate in abbinamento alle caratteristiche individualizzanti virtuali che corrispondono alle caratteristiche individualizzanti reali

Effect of MAPK activation via mutations in NRAS, KRAS and BRAF on clinical outcome in newly diagnosed multiple myeloma.

Until now, next generation sequencing (NGS) data has not been incorporated into any prognostic stratification of multiple myeloma (MM) and no therapeutic considerations are based upon it. In this work, we correlated NGS data with (1) therapy response and survival parameters in newly diagnosed multiple myeloma, treated by VRd * and (2) MM disease stage: newly diagnosed multiple myeloma (ndMM) versus relapsed and/or refractory (relapsed/refractory multiple myeloma). We analyzed 126 patients, with ndMM and relapsed refractory multiple myeloma (rrMM), treated at the University Hospital of Bern (Inselspital). Next generation sequencing was performed on bone marrow, as part of routine diagnostics. The NGS panel comprised eight genes CCND1, DIS3, EGR1, FAM46C (TENT5C), FGFR3, PRDM1, TP53, TRAF3 and seven hotspots in BRAF, IDH1, IDH2, IRF4, KRAS, NRAS. The primary endpoint was complete remission (CR) after VRd in ndMM, in correlation with mutational profile. Mutational load was generally higher in rrMM, with more frequently mutated TP53: 11/87 (13%) in ndMM versus 9/11 (81%) in rrMM (OR 0.0857, p = 0.0007). In ndMM, treated by VRd, mutations in MAPK-pathway members (NRAS, KRAS or BRAF) were associated with reduced probability of CR (21/38, 55%), as compared with wild type NRAS, KRAS or BRAF (34/40, 85%; OR 0.2225, p = 0.006). NRAS c.181C > A (p.Q61K) as a single mutation event showed a trend to reduced probability of achieving CR (OR 0.0912, p = 0.0247). Activation of MAPK pathway via mutated NRAS, KRAS and BRAF genes seems to have a negative impact on outcome in ndMM patients receiving VRd therapy. VRd* - bortezomib (Velcade®), lenalidomide (Revlimid®) and dexamethasone

Correlation of plasma cell assessment by phenotypic methods and molecular profiles by NGS in patients with plasma cell dyscrasias.

BACKGROUND Next-generation sequencing (NGS) detects somatic mutations in a high proportion of plasma cell dyscrasias (PCD), but is currently not integrated into diagnostic routine. We correlated NGS data with degree of bone marrow (BM) involvement by cytomorphology (BMC), histopathology (BMH), and multiparameter flow cytometry (MFC) in 90 PCD patients. METHODS Of the 90 patients the diagnoses comprised multiple myeloma (n = 77), MGUS (n = 7), AL-amyloidosis (n = 4) or solitary plasmocytoma (n = 2). The NGS panel included eight genes CCND1, DIS3, EGR1, FAM46C (TENT5C), FGFR3, PRDM1, TP53, TRAF3, and seven hotspots in BRAF, IDH1, IDH2, IRF4, KRAS, NRAS. RESULTS Mutations were detected in 64/90 (71%) of cases. KRAS (29%), NRAS (16%) and DIS3 (16%) were most frequently mutated. At least one mutation/sample corresponded to a higher degree of BM involvement with a mean of 11% pathologic PC by MFC (range, 0.002-62%), and ~ 50% (3-100%) as defined by both BMC and BMH. CONCLUSIONS The probability of detecting a mutation by NGS in the BM was highest in samples with > 10% clonal PC by MFC, or > 20% PC by BMC/ BMH. We propose further evaluation of these thresholds as a practical cut-off for processing of samples by NGS at initial PCD diagnosis

Comprehensive Laboratory Diagnostic Workup for Patients with Suspected Intraocular Lymphoma including Flow Cytometry, Molecular Genetics and Cytopathology.

BACKGROUND Intraocular lymphoma (IOL) presents a real challenge in daily diagnostics. Cyto- and/or histopathology of vitreous body represent the diagnostic cornerstones. Yet, false negative results remain common. Therefore, we analyzed the diagnostic significance of flow cytometry (FC) within the workup algorithm of IOL and compared its sensitivity with the results obtained from routine cytopathology and molecular genetics; Methods: Seven patients undergoing vitrectomy due to suspected IOL were investigated by FC and parallel cytopathology and, if available, digital droplet PCR (ddPCR) for MYD88 L265P; Results: Four out of seven patients were finally diagnosed with IOL. Among the IOL patients, cytopathology confirmed the presence of lymphoma cells in only two cases. In contrast, FC was positive for IOL in all four cases, and FC additionally confirmed the lack of IOL in the remaining patients. In IOL patients diagnosed by FC and with available ddPCR, the diagnosis of IOL was confirmed by the presence of the MYD88 L265P mutation in all three patients; Conclusions: The combination with FC was superior to cytopathology alone in the diagnostic work-up of IOL, and it showed an excellent correlation with ddPCR results. A comprehensive diagnostic panel consisting of cytopathology, FC and molecular genetics should be considered for the work-up of suspected IOL

Cuts and flows of cell complexes

We study the vector spaces and integer lattices of cuts and flows associated with an arbitrary finite CW complex, and their relationships to group invariants including the critical group of a complex. Our results extend to higher dimension the theory of cuts and flows in graphs, most notably the work of Bacher, de la Harpe and Nagnibeda. We construct explicit bases for the cut and flow spaces, interpret their coefficients topologically, and give sufficient conditions for them to be integral bases of the cut and flow lattices. Second, we determine the precise relationships between the discriminant groups of the cut and flow lattices and the higher critical and cocritical groups with error terms corresponding to torsion (co)homology. As an application, we generalize a result of Kotani and Sunada to give bounds for the complexity, girth, and connectivity of a complex in terms of Hermite's constant.Comment: 30 pages. Final version, to appear in Journal of Algebraic Combinatoric