A EVOLUÇÃO DA CENTRAIS DE MATERIAL E ESTERILIZAÇAO: HISTÓRIA, ATUALIDADES E PERSPECTIVAS PARA A ENFERMAGEM

This study is a theoretical reflection about some aspects of the evolution of the work process at the material sterilization unit that involves the activities of nurses that participate in infection control of the hospital. It was verified that, when making a future projection of the activities in this sector at Brazilian health institutions, the technological and scientific advances for reprocessing medical devices should be considered: The ethical and legal questions, environment preservation and development of protocols adequate to the institutions’ reality, guaranteeing for health systems’ users a riskless and quality assistance.Este estudio es una reflexión teórica que aborda algunos aspectos relacionados con la evolución del proceso de trabajo en centrales de esterilización de material, donde actúan enfermeros que participan del control de las infecciones hospitalarias. Se verificó que al hacer una proyección de las actividades en este sector en el futuro en las instituciones de salud brasileñas, tomando en consideración los avances técnico-científicos en el sector de procesamiento de materiales médico-hospitalarios, deben ser considerados: los aspectos ético-legales, la no agresión al medio ambiente y el desarrollo de protocolos adecuados a la realidad de las instituciones que aseguren a los usuarios de los servicios de salud una asistencia de calidad y libre de riesgos.O estudo trata de uma reflexão teórica que aborda alguns aspectos relacionados à evolução do processo de trabalho em Centrais de Material e Esterilização que perpassa pelas atividades dos enfermeiros no controle de infecções hospitalares. Verificou-se que, ao se fazer uma projeção das atividades neste setor no futuro nas instituições de saúde brasileiras, em vista dos avanços técnico-científicos já alcançados para o reprocessamento de materiais médico-hospitalares, deve-se considerar: as questões éticas e legais, a não-agressão ao meio ambiente e o desenvolvimento de protocolos que sejam adequados à realidade das instituições, mas que assegurem aos usuários dos serviços de saúde uma assistência de qualidade e livre de riscos

Transcriptome profiling of grapevine seedless segregants during berry development reveals candidate genes associated with berry weight

Indexación: Web of Science; PubMedBackground Berry size is considered as one of the main selection criteria in table grape breeding programs. However, this is a quantitative and polygenic trait, and its genetic determination is still poorly understood. Considering its economic importance, it is relevant to determine its genetic architecture and elucidate the mechanisms involved in its expression. To approach this issue, an RNA-Seq experiment based on Illumina platform was performed (14 libraries), including seedless segregants with contrasting phenotypes for berry weight at fruit setting (FST) and 6–8 mm berries (B68) phenological stages. Results A group of 526 differentially expressed (DE) genes were identified, by comparing seedless segregants with contrasting phenotypes for berry weight: 101 genes from the FST stage and 463 from the B68 stage. Also, we integrated differential expression, principal components analysis (PCA), correlations and network co-expression analyses to characterize the transcriptome profiling observed in segregants with contrasting phenotypes for berry weight. After this, 68 DE genes were selected as candidate genes, and seven candidate genes were validated by real time-PCR, confirming their expression profiles. Conclusions We have carried out the first transcriptome analysis focused on table grape seedless segregants with contrasting phenotypes for berry weight. Our findings contributed to the understanding of the mechanisms involved in berry weight determination. Also, this comparative transcriptome profiling revealed candidate genes for berry weight which could be evaluated as selection tools in table grape breeding programs.http://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-016-0789-

The Diffusion of Humans and Cultures in the Course of the Spread of Farming

The most profound change in the relationship between humans and their environment was the introduction of agriculture and pastoralism. [....] For an understanding of the expansion process, it appears appropriate to apply a diffusive model. Broadly, these numerical modeling approaches can be catego- rized in correlative, continuous and discrete. Common to all approaches is the comparison to collections of radiocarbon data that show the apparent wave of advance of the transition to farming. However, these data sets differ in entry density and data quality. Often they disregard local and regional specifics and research gaps, or dating uncertainties. Thus, most of these data bases may only be used on a very general, broad scale. One of the pitfalls of using irregularly spaced or irregularly documented radiocarbon data becomes evident from the map generated by Fort (this volume, Chapter 16): while the general east-west and south-north trends become evident, some areas appear as having undergone anomalously early transitions to farming. This may be due to faulty entries into the data base or regional problems with radiocarbon dating, if not unnoticed or undocumented laboratory mistakes.Comment: 20 pages, 5 figures, submitted to Diffusive Spreading in Nature, Technology and Society, edited by Armin Bunde, J\"urgen Caro, J\"org K\"arger, Gero Vogl, Chapter 1

RETENÇÃO PROLONGADA DOS DENTES DECÍDUOS

A retenção prolongada de um dente decíduo é a sua permanência no arco dentário após o período esperado para sua esfoliação, pode ocorrer por falta de espaço da erupção do permanente, posicionamento inadequado do germe sucessor, ausência do germe permanente, presença de supranumerários e anquilose dos dentes decíduos, segundo Guedes Pinto (1995)

Tin(iv) dopant removal through anti-solvent engineering enabling tin based perovskite solar cells with high charge carrier mobilities

We report the need for careful selection of anti-solvents for Sn-based perovskite solar cells fabricated through the commonly used anti-solvent method, compared to their Pb-based counterparts.</p

Tissue Localization and Extracellular Matrix Degradation by PI, PII and PIII Snake Venom Metalloproteinases: Clues on the Mechanisms of Venom-Induced Hemorrhage

20 páginas, 4 figuras, 3 tablas y 7 tablas en material suplementario.Snake venom hemorrhagic metalloproteinases (SVMPs) of the PI, PII and PIII classes were compared in terms of tissue localization and their ability to hydrolyze basement membrane components in vivo, as well as by a proteomics analysis of exudates collected in tissue injected with these enzymes. Immunohistochemical analyses of co-localization of these SVMPs with type IV collagen revealed that PII and PIII enzymes co-localized with type IV collagen in capillaries, arterioles and post-capillary venules to a higher extent than PI SVMP, which showed a more widespread distribution in the tissue. The patterns of hydrolysis by these three SVMPs of laminin, type VI collagen and nidogen in vivo greatly differ, whereas the three enzymes showed a similar pattern of degradation of type IV collagen, supporting the concept that hydrolysis of this component is critical for the destabilization of microvessel structure leading to hemorrhage. Proteomic analysis of wound exudate revealed similarities and differences between the action of the three SVMPs. Higher extent of proteolysis was observed for the PI enzyme regarding several extracellular matrix components and fibrinogen, whereas exudates from mice injected with PII and PIII SVMPs had higher amounts of some intracellular proteins. Our results provide novel clues for understanding the mechanisms by which SVMPs induce damage to the microvasculature and generate hemorrhage.This work was performed in partial fulfillment of the requirements for the PhD degree for Cristina Herrera at Universidad de Costa Rica.Peer reviewe