Normal Ribosomal Biogenesis but Shortened Protein Synthetic Response to Acute Eccentric Resistance Exercise in Old Skeletal Muscle.

Anabolic resistance to feeding in aged muscle is well-characterized; however, whether old skeletal muscle is intrinsically resistant to acute mechanical loading is less clear. The aim of this study was to determine the impact of aging on muscle protein synthesis (MPS), ribosome biogenesis, and protein breakdown in skeletal muscle following a single bout of resistance exercise. Adult male F344/BN rats aged 10 (Adult) and 30 (Old) months underwent unilateral maximal eccentric contractions of the hindlimb. Precursor rRNA increased early post-exercise (6-18 h), preceding elevations in ribosomal mass at 48 h in Adult and Old; there were no age-related differences in these responses. MPS increased early post-exercise in both Adult and Old; however, at 48 h of recovery, MPS returned to baseline in Old but not Adult. This abbreviated protein synthesis response in Old was associated with decreased levels of IRS1 protein and increased BiP, CHOP and eIF2α levels. Other than these responses, anabolic signaling was similar in Adult and Old muscle in the acute recovery phase. Basal proteasome activity was lower in Old, and resistance exercise did not increase the activity of either the ATP-dependent or independent proteasome, or autophagy (Cathepsin L activity) in either Adult or Old muscle. We conclude that MPS and ribosome biogenesis in response to maximal resistance exercise in old skeletal muscle are initially intact; however, the MPS response is abbreviated in Old, which may be the result of ER stress and/or blunted exercise-induced potentiation of the MPS response to feeding

Model-based electron density profile estimation and control, applied to ITER

In contemporary magnetic confinement devices, the density distribution is sensed with interferometers and actuated with feedback controlled gas injection and open-loop pellet injection. This is at variance with the density control for ITER and DEMO, that will depend mainly on pellet injection as an actuator in feed-back control. This paper presents recent developments in state estimation and control of the electron density profile for ITER using relevant sensors and actuators. As a first step, Thomson scattering is included in an existing dynamic state observer. Second, model predictive control is developed as a strategy to regulate the density profile while avoiding limits associated with the total density (Greenwald limit) or gradients in the density distribution (e.g. neo-classical impurity transport). Simulations show that high quality density profile estimation can be achieved with Thomson Scattering and that the controller is capable of regulating the distribution as desired

Neodymium isotopic composition and concentration in the western North Atlantic Ocean: results from the GEOTRACES GA02 section

The neodymium (Nd) isotopic composition of seawater is commonly used as a proxy to study past changes in the thermohaline circulation. The modern database for such reconstructions is however poor and the understanding of the underlying processes is incomplete. Here we present new observational data for Nd isotopes and concentrations from twelve seawater depth profiles, which follow the flow path of North Atlantic Deep Water (NADW) from its formation region in the North Atlantic to the northern equatorial Atlantic. Samples were collected during two cruises constituting the northern part of the Dutch GEOTRACES transect GA02 in 2010. The results show that the different water masses in the subpolar North Atlantic Ocean, which ultimately constitute NADW, have the following Nd isotope characteristics: Upper Labrador Sea Water (ULSW), εNd = -14.2 ± 0.3; Labrador Sea Water (LSW), εNd = -13.7 ± 0.9; Northeast Atlantic Deep Water (NEADW), εNd = -12.5 ± 0.6; Northwest Atlantic Bottom Water (NWABW), εNd = -11.8 ± 1.4. In the subtropics, where these source water masses have mixed to form NADW, which is exported to the global ocean, upper-NADW is characterised by εNd values of -13.2 ± 1.0 (2sd) and lower-NADW exhibits values of εNd = -12.4 ± 0.4 (2sd). While both signatures overlap within error, the signature for lower-NADW is significantly more radiogenic than the traditionally used value for NADW (εNd = -13.5) due to the dominance of source waters from the Nordic Seas (NWABW and NEADW). Comparison between the concentration profiles and the corresponding Nd isotope profiles with other water mass properties such as salinity, silicate concentrations, neutral densities and chlorofluorocarbon (CFC) concentration provides novel insights into the geochemical cycle of Nd and reveals that different processes are necessary to account for the observed Nd characteristics in the subpolar and subtropical gyres and throughout the vertical water column. While our data set provides additional insights into the contribution of boundary exchange in areas of sediment resuspension, the results for open ocean seawater demonstrate, at an unprecedented level, the suitability of Nd isotopes to trace modern water masses in the strongly advecting western Atlantic Ocean

Inter-relatedness of underlying factors for injury and violence among adolescents in rural coastal Kenya: A qualitative study

We utilized a socio-ecological model to explore views from 85 young people and 10 local stakeholders on forms and underlying factors for unintentional injury, violence, self-harm, and suicidal behavior of adolescents in Kilifi County, Kenya. Young people took part in 11 focus group discussions, whereas 10 in-depth interviews were conducted with the local stakeholders. Road traffic accidents, falls, fights, sexual and gender-based violence, theft, and vandalism were viewed as common. There was an overlap of risk factors, especially at intra- and interpersonal levels (gender, poverty, substance use, parenting behavior, school drop-out). Some broader-level risk factors were insecure neighborhoods and risky sources of livelihood. Research is needed to quantify burden and to pilot feasible injury prevention interventions in this setting

Discovery Prospects for a Supernova Signature of Biogenic Origin

Approximately 2.8 Myr before the present our planet was subjected to the debris of a supernova explosion. The terrestrial proxy for this event was the discovery of live atoms of 60Fe in a deep-sea ferromanganese crust. The signature for this supernova event should also reside in magnetite Fe3O4 microfossils produced by magnetotactic bacteria extant at the time of the Earth-supernova interaction, provided the bacteria preferentially uptake iron from fine-grained iron oxides and ferric hydroxides. Using estimates for the terrestrial supernova 60Fe flux, combined with our empirically derived microfossil concentrations in a deep-sea drill core, we deduce a conservative estimate of the ^{60}{Fe} fraction as 60Fe/Fe ~ 3.6 x 10^{-15}. This value sits comfortably within the sensitivity limit of present accelerator mass spectrometry capabilities. The implication is that a biogenic signature of this cosmic event is detectable in the Earth's fossil record.Comment: As it appears in Icaru

Molecular brakes regulating mTORC1 activation in skeletal muscle following synergist ablation

The goal of the current work was to profile positive (mTORC1 activation, autocrine/paracrine growth factors) and negative [AMPK, unfolded protein response (UPR)] pathways that might regulate overload-induced mTORC1 (mTOR complex 1) activation with the hypothesis that a number of negative regulators of mTORC1 will be engaged during a supraphysiological model of hypertrophy. To achieve this, mTORC1- IRS-1/2 signaling, BiP/CHOP/IRE1, and AMPK activation were determined in rat plantaris muscle following synergist ablation (SA). SA resulted in significant increases in muscle mass of 4% per day throughout the 21 days of the experiment. The expression of the insulin-like growth factors (IGF) were high throughout the 21st day of overload. However, IGF signaling was limited, since IRS-1 and -2 were undetectable in the overloaded muscle from day 3 to day 9. The decreases in IRS-1/2 protein were paralleled by increases in GRB10 Ser501/503 and S6K1 Thr389 phosphorylation, two mTORC1 targets that can destabilize IRS proteins. PKB Ser473 phosphorylation was higher from 3– 6 days, and this was associated with increased TSC2 Thr939 phosphorylation. The phosphorylation of TSC2 Thr1345 (an AMPK site) was also elevated, whereas phosphorylation at the other PKB site, Thr1462, was unchanged at 6 days. In agreement with the phosphorylation of Thr1345, SA led to activation of AMPK1 during the initial growth phase, lasting the first 9 days before returning to baseline by day 12. The UPR markers CHOP and BiP were elevated over the first 12 days following ablation, whereas IRE1 levels decreased. These data suggest that during supraphysiological muscle loading at least three potential molecular brakes engage to downregulate mTORC1. m

Scour holes and ripples occur below the hydraulic smooth to rough transition of movable beds

© 2017 The Authors. Scour holes often form in shallow flows over sand on the beach and in morphodynamic scale experiments of river reaches, deltas and estuarine landscapes. The scour holes are on average 2cm deep and 5cm long, regardless of the flow depth and appear to occur under similar conditions as current ripples: at low boundary Reynolds numbers, in fine sand and under relatively low sediment mobility. In landscape experiments, where the flow is only about 1cm deep, such scours may be unrealistically large and have unnatural effects on channel formation, bar pattern and stratigraphy. This study tests the hypotheses that both scours and ripples occur in the same conditions and that the roughness added by sediment saltation explains the difference between the ripple–dune transition and the clear-water hydraulic smooth to rough transition. About 500 experiments are presented with a range of sediment types, sediment mobility and obstructions to provoke scour holes, or removal thereof to assess scour hole persistence. Most experiments confirm that ripples and scour holes both form in the ripple stability field in two different bedform stability diagrams. The experiments also show that scours can be provoked by perturbations even below generalized sediment motion. Moreover, the hydraulic smooth to rough transition modified with saltation roughness depending on sediment mobility was similar in magnitude and in slope to ripple–dune transitions. Given uncertainties in saltation relations, the smooth to rough transitions modified for movable beds are empirically equivalent to the ripple–dune transitions. These results are in agreement with the hypothesis that scours form by turbulence caused by localized flow separation under low boundary Reynolds numbers, and do not form under generalized flow separation over coarser particles and intense sediment saltation. Furthermore, this suggests that ripples are a superposition of two independent forms: periodic bedforms occurring in smooth and rough conditions plus aperiodic scours occurring only in hydraulic smooth conditions

Dissolved aluminium in the ocean conveyor of theWest Atlantic Ocean: Effects of the biological cycle, scavenging, sediment resuspension and hydrography

The concentrations of dissolved aluminium (dissolved Al) were studied along the West Atlantic GEOTRACESGA02 transect from 64°N to 50°S. Concentrations ranged from~0.5 nmol kg-1 in the high latitude surface watersto ~48 nmol kg-1 in surfacewaters around 25°N. Elevated surfacewater concentrations due to atmospheric dustloading have little influence on the deep water distribution. However, just belowthe thermocline, both Northernand Southern Hemisphere Subtropical Mode Waters are elevated in Al, most likely related to atmospheric dustdeposition in the respective source regions.In the deep ocean, high concentrations of up to 35 nmol kg-1 were observed in North Atlantic DeepWater as aresult of Al input via sediment resuspension. Comparatively lowdeepwater concentrationswere associatedwithwater masses of Antarctic origin. During water mass advection, Al loss by scavenging overrules input viaremineralisation and sediment resuspension at the basin wide scale. Nevertheless, sediment resuspension ismore important than previously realised for the deep ocean Al distribution and even more intensive samplingis needed in bottom waters to constrain the spatial heterogeneity in the global deep ocean.This thus far longest (17,500 km) full depth ocean section shows that the distribution of Al can be explained by itsinput sources and the combination of association with particles and release from those particles at depth, thelattermost likelywhen the particles remineralise. The association of Alwith particles can be due to incorporationof Al into biogenic silica or scavenging of Al onto biogenic particles. The interaction between Al and biogenicparticles can lead to the coupled cycling of Al and silicate that is observed in some ocean regions. However, inother regions this coupling is not observed due to (i) advective processes bringing in older water masses thatare depleted in Al, (ii) unfavourable scavenging conditions in the water column, (iii) low surface concentrationsof Al or (iv) additional Al sources, notably sediment resuspension

Morphological effects of vegetation on the tidal-fluvial transition in Holocene estuaries

Vegetation enhances bank stability and sedimentation to such an extent that it can modify river patterns, but how these processes manifest themselves in full-scale estuarine settings is poorly understood. On the one hand, tidal flats accrete faster in the presence of vegetation, reducing the flood storage and ebb dominance over time. On the other hand flow-focusing effects of a tidal floodplain elevated by mud and vegetation could lead to channel concentration and incision. Here we study isolated and combined effects of mud and tidal marsh vegetation on estuary dimensions. A 2-D hydromorphodynamic estuary model was developed, which was coupled to a vegetation model and used to simulate 100 years of morphological development. Vegetation settlement, growth and mortality were determined by the hydromorphodynamics. Eco-engineering effects of vegetation on the physical system are here limited to hydraulic resistance, which affects erosion and sedimentation pattern through the flow field. We investigated how vegetation, combined with mud, affects the average elevation of tidal flats and controls the system-scale planform. Modelling with vegetation only results in a pattern with the largest vegetation extent in the mixed-energy zone of the estuary, which is generally shallower. Here vegetation can cover more than 50 % of the estuary width while it remains below 10 %–20 % in the outer, tide-dominated zone. This modelled distribution of vegetation along the estuary shows general agreement with trends in natural estuaries observed by aerial image analysis. Without mud, the modelled vegetation has a limited effect on morphology, again peaking in the mixed-energy zone. Numerical modelling with mud only shows that the presence of mud leads to stabilisation and accretion of the intertidal area and a slight infill of the mixed-energy zone. Combined modelling of mud and vegetation leads to mutual enhancement with mud causing new colonisation areas and vegetation stabilising the mud. This occurs in particular in a zone previously described as the bedload convergence zone. While vegetation focusses the flow into the channels such that mud sedimentation in intertidal side channels is prevented on a timescale of decades, the filling of intertidal area and the resulting reduction in tidal prism may cause the infilling of estuaries over centuries