A regional ground motion excitation/attenuation model for the San Francisco region

By using small-to-moderate-sized earthquakes located within ~200 km of San Francisco, we characterize the scaling of the ground motions for frequencies ranging between 0.25 and 20 Hz, obtaining results for geometric spreading, Q(f), and site parameters using the methods of Mayeda et al. (2005) and Malagnini et al. (2004). The results of the analysis show that, throughout the Bay Area, the average regional attenuation of the ground motion can be modeled with a bilinear geometric spreading function with a 30 km crossover distance, coupled to an anelastic function exp(-pi*f*r/V*Q(f)) , where: Q(f)=180f^0.42. A body-wave geometric spreading, g(r)= r^-1.0, is used at short hypocentral distances (r < 30 km), whereas g(r)= r^-0.6 fits the attenuation of the spectral amplitudes at hypocentral distances beyond the crossover. The frequency-dependent site effects at 12 of the Berkeley Digital Seismic Network (BDSN) stations were evaluated in an absolute sense using coda-derived source spectra. Our results show: i) the absolute site response for frequencies ranging between 0.3 Hz and 2.0 Hz correlate with independent estimates of the local magnitude residuals (dML) for each of the stations; ii) moment-magnitudes (MW) derived from our path and site-corrected spectra are in excellent agreement with those independently derived using full-waveform modeling as well as coda-derived source spectra; iii) we use our weak-motion-based relationships to predict motions region wide for the Loma Prieta earthquake, well above the maximum magnitude spanned by our data set, on a completely different set of stations. Results compare well with measurements taken at specific NEHRP site classes; iv) an empirical, magnitude-dependent scaling was necessary for the Brune stress parameter in order to match the large magnitude spectral accelerations and peak ground velocities with our weak-motion-based model

A Regional Ground Motion Excitation attenuation Model for the San Francisco Region

By using small-to-moderate-sized earthquakes located within ~200 km of San Francisco, we characterize the scaling of the ground motions for frequencies ranging between 0.25 and 20 Hz, obtaining results for geometric spreading, Q(f), and site parameters using the methods of Mayeda et al. (2005) and Malagnini et al. (2004). The results of the analysis show that, throughout the Bay Area, the average regional attenuation of the ground motion can be modeled with a bilinear geometric spreading function with a 30 km crossover distance, coupled to an anelastic function ! exp " #fr $Q( f ) % & ' ( ) * , where: Q(f)=180 f 0.42. A body-wave geometric spreading, g(r)= r -1.0, is used at short hypocentral distances (r < 30 km), whereas g(r)= r -0.6 fits the attenuation of the spectral amplitudes at hypocentral distances beyond the crossover. The frequency-dependent site effects at 12 of the Berkeley Digital Seismic Network (BDSN) stations were evaluated in an absolute sense using coda-derived source spectra. Our results show: i) the absolute site response for frequencies ranging between 0.3 Hz and 2.0 Hz correlate with independent estimates of the local magnitude residuals (dML) for each of the stations; ii) moment-magnitudes (MW) derived from our path and sitecorrected spectra are in excellent agreement with those independently derived using fullwaveform modeling as well as coda-derived source spectra; iii) we use our weak-motionbased relationships to predict motions region wide for the Loma Prieta earthquake, well above the maximum magnitude spanned by our data set, on a completely different set of stations. Results compare well with measurements taken at specific NEHRP site classes; iv) an empirical, magnitude-dependent scaling was necessary for the Brune stress parameter in order to match the large magnitude spectral accelerations and peak ground velocities with our weak-motion-based model

Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors

The SR proteins are essential metazoan pre-mRNA splicing factors that can also influence the selection of alternative 5' splice sites in a concentration-dependent manner. Their activity in alternative splicing in vitro is antagonized by members of the hnRNP A/B family of proteins. The opposite effects of members of these two families of antagonistic splicing factors in vitro and upon overexpression in vivo suggest that changes in their relative levels may be a natural mechanism for the regulation of alternative splicing in vivo. One prediction of this model is that the ratios of these antagonists should vary in different cell types and in other situations in which cellular or viral transcripts are differentially spliced. We raised monoclonal antibodies specific for SFS/ASF and used them to measure the abundance of SFS/ASF protein and its isoforms, its phosphorylation state in vivo and during splicing in vitro, and its association with the spliceosome. SF2/ASF exists predominantly or exclusively in a highly phosphorylated state in vivo in all cell types examined, and unphosphorylated protein was not detectable. Unphosphorylated recombinant SFS/ASF becomes rapidly phosphorylated under splicing conditions in HeLa cell extracts and associates stably with one or more exons of beta-globin pre-mRNA. This interaction appears to persist through the splicing reaction and SF2/ASF remains bound to spliced mRNA. We compared the distribution of SFS/ASF to that of its antagonist, hnRNP Al, in different rat tissues and in immortal and transformed cell lines. We found that the protein levels of these antagonistic splicing factors vary naturally over a very wide range, supporting the notion that changes in the ratio of these proteins can affect alternative splicing of a variety of pre-mRNAs in vivo

Identifying the Independent Inertial Parameter Space of Robot Manipulators

This paper presents a new approach to the problem of finding the minimum number of inertial parameters of robot manipulator dynamic equations of motion. Based upon the energy difference equation, it is equally applica ble to serial link manipulators as well as graph structured manipulators. The method is conceptually simple, compu tationally efficient, and easy to implement. In particular, the manipulator kinematics and the joint positions and velocities are the only inputs to the algorithm. Applica tions to a serial link and a graph structured manipulator are illustrated.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67982/2/10.1177_027836499101000606.pd

"It is what it is": masculinity, homosexuality, and inclusive discourse in mixed martial arts

In this paper we make use of inclusive masculinity theory to explore online media representations of male homosexuality and masculinity within the increasingly popular combat sport of mixed martial arts MMA). Adopting a case-study approach, we discuss narratives constructed around one aspirational male MMA fighter, Dakota Cochrane, whose history of having participated in gay pornography became a major talking point on a number of MMA 'fanzine'/'community' websites during early 2012. While these narratives attempted to discursively 'rescue' Cochrane's supposedly threatened masculinity, highlighting both his 'true' heterosexuality and his prodigious fighting abilities, they also simultaneously celebrated the acceptance of homosexual men within the sport which Cochrane's case implied. Thus, we suggest that these media representations of homosexuality and masculinity within MMA are indicative of declining cultural homophobia and homohysteria, and an inclusive vision of masculinity, as previously described by proponents of inclusive masculinity theory

Thermal Links for the Implementation of an Optical Refrigerator

Optical refrigeration has been demonstrated by several groups of researchers, but the cooling elements have not been thermally linked to realistic heat loads in ways that achieve the desired temperatures. The ideal thermal link will have minimal surface area, provide complete optical isolation for the load, and possess high thermal conductivity. We have designed thermal links that minimize the absorption of fluoresced photons by the heat load using multiple mirrors and geometric shapes including a hemisphere, a kinked waveguide, and a tapered waveguide. While total link performance is dependent on additional factors, we have observed net transmission of photons with the tapered link as low as 0.04%. Our optical tests have been performed with a surrogate source that operates at 625 nm and mimics the angular distribution of light emitted from the cooling element of the Los Alamos solid state optical refrigerator. We have confirmed the optical performance of our various link geometries with computer simulations using CODE V optical modeling software. In addition we have used the thermal modeling tool in COMSOL MULTIPHYSICS to investigate other heating factors that affect the thermal performance of the optical refrigerator. Assuming an ideal cooling element and a nonabsorptive dielectric trapping mirror, the three dominant heating factors are (1) absorption of fluoresced photons transmitted through the thermal link, (2) blackbody radiation from the surrounding environment, and (3) conductive heat transfer through mechanical supports. Modeling results show that a 1 cm3 load can be chilled to 107 K with a 100Wpump laser. We have used the simulated steady-state cooling temperatures of the heat load to compare link designs and system configurations

Gender, media, and mixed martial arts in Poland: the case of Joanna Jędrzejczyk

Recent growth in the media visibility of female combat sport athletes has offered a compelling site for research on gender and sport media, as women in deeply masculinized sports have been increasingly placed in the public spotlight. While scholars in the Anglophone West have offered analyses of the media framing of this phenomenon, little work has been done outside these cultural contexts. Thus, in this paper we offer a qualitative exploration of how Joanna Jędrzejczyk, a Polish champion of the Ultimate Fighting Championship, has been represented in Polish media. Our findings reveal a relatively de-gendered, widely celebratory account, primarily framed by nationalistic discourse–findings we ascribe to both the particularities of the sport of mixed martial arts as well as the historic nature of Jędrzejczyk’s success

Alternative splicing: an important mechanism for myometrial gene regulation that can be manipulated to target specific genes associated with preterm labour

Considerable effort has been expended in attempting to distinguish genes that contribute to initiating the onset of term and preterm labour (PTL) from those that change in expression as a consequence of the progression of labour. The ability to define more clearly the genes involved in triggering labour contractions should lead to the development of new effective and safer strategies to prevent preterm birth. There is ample evidence to suggest that specific genes are co-ordinately regulated within the upper and lower regions of the myometrium prior to and during parturition and many of these genes are regulated by alternative pre-mRNA splicing. This mini-review highlights that expression of a range of different splicing factors, with defined roles in pre-mRNA splicing, is both temporally and spatially regulated within the uterine smooth muscle during pregnancy and labour. Moreover, several of these splicing factors play key roles in controlling the differential expression of specific regulatory proteins involved in uterine signalling and uterine quiescence. In addition, antisense morpholino oligonucleotide manipulation of pre-mRNA splicing may have potential in defining and targeting uterine pro-labour genes and thus contribute to the development of new therapeutic approaches to prevent PTL

Structural basis for terminal loop recognition and stimulation of pri-miRNA-18a processing by hnRNP A1

International audiencePost-transcriptional mechanisms play a predominant role in the control of microRNA (miRNA) production. Recognition of the terminal loop of precursor miRNAs by RNA-binding proteins (RBPs) influences their processing; however, the mechanistic basis for how levels of individual or subsets of miRNAs are regulated is mostly unexplored. We previously showed that hnRNP A1, an RBP implicated in many aspects of RNA processing, acts as an auxiliary factor that promotes the Microprocessor-mediated processing of pri-mir-18a. Here, by using an integrative structural biology approach, we show that hnRNP A1 forms a 1:1 complex with pri-mir-18a where both RNA recognition motifs (RRMs) bind to cognate RNA sequence motifs in the terminal loop of pri-mir-18a. Terminal loop binding induces an allosteric destabilization of base-pairing in the pri-mir-18a stem that promotes its downstream processing. Our results highlight terminal loop RNA recognition by RBPs as a potential general principle of miRNA biogenesis and regulation

Primordial Compositions of Refractory Inclusions

Bulk chemical and oxygen, magnesium and silicon isotopic compositions were measured for each of 17 Types A and B refractory inclusions from CV3 chondrites. After bulk chemical compositions were corrected for non-representative sampling in the laboratory, the Mg and Si isotopic compositions of each inclusion were used to calculate its original chemical composition assuming that the heavy-isotope enrichments of these elements are due to Rayleigh fractionation that accompanied their evaporation from CMAS liquids. The resulting pre-evaporation chemical compositions are consistent with those predicted by equilibrium thermodynamic calculations for high-temperature nebular condensates but only if different inclusions condensed from nebular regions that ranged in total pressure from 10{sup -6} to 10{sup -1} bar, regardless of whether they formed in a system of solar composition or in one enriched in OC dust relative to gas by a factor of ten relative to solar composition. This is similar to the range of total pressures predicted by dynamic models of the solar nebula for regions whose temperatures are in the range of silicate condensation temperatures. Alternatively, if departure from equilibrium condensation and/or non-representative sampling of condensates in the nebula occurred, the inferred range of total pressure could be smaller. Simple kinetic modeling of evaporation successfully reproduces observed chemical compositions of most inclusions from their inferred pre-evaporation compositions, suggesting that closed-system isotopic exchange processes did not have a significant effect on their isotopic compositions. Comparison of pre-evaporation compositions with observed ones indicates that 80% of the enrichment in refractory CaO + Al{sub 2}O{sub 3} relative to more volatile MgO + SiO{sub 2} is due to initial condensation and 20% due to subsequent evaporation for both Type A and Type B inclusions