High cadence spectropolarimetry of moving magnetic features observed around a pore

Moving magnetic features (MMFs) are small-size magnetic elements that are seen to stream out from sunspots, generally during their decay phase. Several observational results presented in the literature suggest them to be closely related to magnetic filaments that extend from the penumbra of the parent spot. Nevertheless, few observations of MMFs streaming out from spots without penumbra have been reported. The literature still lacks of analyses of the physical properties of these features. We investigate physical properties of monopolar MMFs observed around a small pore that had developed penumbra in the days preceding our observations and compare our results with those reported in the literature for features observed around sunspots. We analyzed NOAA 11005 during its decay phase with data acquired at the Dunn Solar Telescope in the FeI 617.3$nm and the CaII 854.2$ nm spectral lines with IBIS, and in the G-band. The field of view showed monopolar MMFs of both polarities streaming out from the leading negative polarity pore of the observed active region. Combining different analyses of the data, we investigated the temporal evolution of the relevant physical quantities associated with the MMFs as well as the photospheric and chromospheric signatures of these features. We show that the characteristics of the investigated MMFs agree with those reported in the literature for MMFs that stream out from spots with penumbrae. Moreover, observations of at least two of the observed features suggest them to be manifestations of emerging magnetic arches.Comment: Accepted by A&

The S phase checkpoint promotes the Smc5/6 complex dependent SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε

Replication fork stalling and accumulation of single-stranded DNA trigger the S phase checkpoint, a signalling cascade that, in budding yeast, leads to the activation of the Rad53 kinase. Rad53 is essential in maintaining cell viability, but its targets of regulation are still partially unknown. Here we show that Rad53 drives the hyper-SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε, principally following replication forks stalling induced by nucleotide depletion. Pol2 is the main target of SUMOylation within the replisome and its modification requires the SUMO-ligase Mms21, a subunit of the Smc5/6 complex. Moreover, the Smc5/6 complex co-purifies with Pol ε, independently of other replisome components. Finally, we map Pol2 SUMOylation to a single site within the N-terminal catalytic domain and identify a SUMO-interacting motif at the C-terminus of Pol2. These data suggest that the S phase checkpoint regulate Pol ε during replication stress through Pol2 SUMOylation and SUMO-binding abilit

Effects of Environmental Factors on Severity and Mortality of COVID-19

Background: Most respiratory viruses show pronounced seasonality, but for SARS-CoV-2, this still needs to be documented. Methods: We examined the disease progression of COVID-19 in 6,914 patients admitted to hospitals in Europe and China. In addition, we evaluated progress of disease symptoms in 37,187 individuals reporting symptoms into the COVID Symptom Study application. Findings: Meta-analysis of the mortality risk in seven European hospitals estimated odds ratios per 1-day increase in the admission date to be 0.981 (0.973–0.988, p < 0.001) and per increase in ambient temperature of 1°C to be 0.854 (0.773–0.944, p = 0.007). Statistically significant decreases of comparable magnitude in median hospital stay, probability of transfer to the intensive care unit, and need for mechanical ventilation were also observed in most, but not all hospitals. The analysis of individually reported symptoms of 37,187 individuals in the UK also showed the decrease in symptom duration and disease severity with time. Interpretation: Severity of COVID-19 in Europe decreased significantly between March and May and the seasonality of COVID-19 is the most likely explanation

Aortic stiffness is associated with cardiac function and cerebral small vessel disease in patients with type 1 diabetes mellitus: assessment by magnetic resonance imaging

To evaluate, with the use of magnetic resonance imaging (MRI), whether aortic pulse wave velocity (PWV) is associated with cardiac left ventricular (LV) function and mass as well as with cerebral small vessel disease in patients with type 1 diabetes mellitus (DM). We included 86 consecutive type 1 DM patients (49 male, mean age 46.9 +/- 11.7 years) in a prospective, cross-sectional study. Exclusion criteria included aortic/heart disease and general MRI contra-indications. MRI of the aorta, heart and brain was performed for assessment of aortic PWV, as a marker of aortic stiffness, systolic LV function and mass, as well as for the presence of cerebral white matter hyperintensities (WMHs), microbleeds and lacunar infarcts. Multivariate linear or logistic regression was performed to analyse the association between aortic PWV and outcome parameters, with covariates defined as age, gender, mean arterial pressure, heart rate, BMI, smoking, DM duration and hypertension. Mean aortic PWV was 7.1 +/- 2.5 m/s. Aortic PWV was independently associated with LV ejection fraction ( = -0.406, P = 0.006), LV stroke volume ( = -0.407, P = 0.001), LV cardiac output ( = -0.458, P = 0.001), and with cerebral WMHs (P < 0.05). There were no independent associations between aortic stiffness and LV mass, cerebral microbleeds or lacunar infarcts. Aortic stiffness is independently associated with systolic LV function and cerebral WMHs in patients with type 1 DM.Neuro Imaging Researc

Blast-induced liquefaction in silty sands for full-scale testing of ground improvement methods: Insights from a multidisciplinary study

In the engineering geology field increased attention has been posed in recent years to potential liquefaction mitigation interventions in susceptible sand formations. In silty sands this is a major challenge because, as the fines content increases, vibratory methods for densification become progressively less effective. An alternative mitigation technique can be the installation of Rammed Aggregate Pier\uae (RAP) columns that can increase the resistance of the soil, accounting for its lateral stress increase and for the stiffness increase from soil and RAP composite response. To investigate the influence of these factors on liquefaction resistance, full-scale blast tests were performed at a silty sand site in Bondeno (Ferrara, Italy) where liquefaction was observed after the 2012 Emilia-Romagna earthquake. A multidisciplinary team of forty researchers carried out devoted experimental activities aimed at better understanding the liquefaction process at the field scale and the effectiveness of the treatment using inter-related methods. Both natural and improved areas were investigated by in-situ tests and later subjected to controlled blasting. The blast tests were monitored with geotechnical and geophysical instrumentation, topographical surveying and geological analyses on the sand boils. Results showed the RAP effectiveness due to the improvement of soil properties within the liquefiable layer and a consequent reduction of the blast-induced liquefaction settlements, likely due to soil densification and increased lateral stress. The applied multidisciplinary approach adopted for the study allowed better understanding of the mechanism involved in the liquefaction mitigation intervention and provided a better overall evaluation of mitigation effectiveness

Phenylketonuria in Portugal: Genotype-Phenotype Correlations Using Molecular, Biochemical, and Haplotypic Analyses

The impairment of the hepatic enzyme phenylalanine hydroxylase (PAH) causes elevation of phenylalanine levels in blood and other body fluids resulting in the most common inborn error of amino acid metabolism (phenylketonuria). Persistently high levels of phenylalanine lead to irreversible damage to the nervous system. Therefore, early diagnosis of the affected individuals is important, as it can prevent clinical manifestations of the disease.info:eu-repo/semantics/publishedVersio

Optimization of Suture-Free Laser-Assisted Vessel Repair by Solder-Doped Electrospun Poly(ε-caprolactone) Scaffold

Poor welding strength constitutes an obstacle in the clinical employment of laser-assisted vascular repair (LAVR) and anastomosis. We therefore investigated the feasibility of using electrospun poly(ε-caprolactone) (PCL) scaffold as reinforcement material in LAVR of medium-sized vessels. In vitro solder-doped scaffold LAVR (ssLAVR) was performed on porcine carotid arteries or abdominal aortas using a 670-nm diode laser, a solder composed of 50% bovine serum albumin and 0.5% methylene blue, and electrospun PCL scaffolds. The correlation between leaking point pressures (LPPs) and arterial diameter, the extent of thermal damage, structural and mechanical alterations of the scaffold following ssLAVR, and the weak point were investigated. A strong negative correlation existed between LPP and vessel diameter, albeit LPP (484 ± 111 mmHg) remained well above pathophysiological pressures. Histological analysis revealed that thermal damage extended into the medial layer with a well-preserved internal elastic lamina and endothelial cells. Laser irradiation of PCL fibers and coagulation of solder material resulted in a strong and stiff scaffold. The weak point of the ssLAVR modality was predominantly characterized by cohesive failure. In conclusion, ssLAVR produced supraphysiological LPPs and limited tissue damage. Despite heat-induced structural/mechanical alterations of the scaffold, PCL is a suitable polymer for weld reinforcement in medium-sized vessel ssLAVR