Tipologías de consumidores de alcohol dentro de la práctica del botellón en tres ciudades españolas.

En este trabajo se entrevistó a 6.009 jóvenes que practican botellón (14-25 años) en tres ciudades de la Comunidad Valenciana, realizando un muestreo estratifi cado de centros de Secundaria, Bachiller,CF y Universidad. Se efectuó un análisis de conglomerados en dos fases con cada muestra con dos objetivos: identifi car tipologías de consumidores de alcohol en el botellón y realizar una validación cruzada de la solución obtenida. Las variables consideradas en los análisis fueron: sexo, grupo de edad (universitarios: U; estudiantes Secundaria: ES), realización o no de episodios intensivos de consumo, años practicando botellón y gramos de alcohol ingeridos. Los resultados muestran evidencia de validez de la estructura obtenida para los jóvenes que realizan episodios intensivos de consumo de alcohol, ya que aparece una estructura de cuatro grupos en las tres muestras (varones U, varones ES, mujeres U,mujeres ES), siendo siempre los varones quienes más consumen. Además, los ES consumen iguales cantidades de hol que los U, aunque llevan menos años consumiendo. Por su parte, entre los jóvenes que no realizan episodios intensivos de consumo de alcohol se observa cierta variabilidad en la estructura, siendo manifi esta la tendencia de las mujeres a igualar su consumo con el de los varones. Different typologies of alcohol consumers in the practice of «botellon» in three Spanish cities. In this work, 6,009 youngsters (14-25 years old) who practice the «botellon» were interviewed in three cities of the Valencian Community. After a stratifi ed sampling among college and noncollege students, a two-step cluster analysis was carried out for each sample with two aims: to identify different types of alcohol consumers in the practice of the «botellon» and to carry out a crossed validation of the results obtained. The variables included in the analysis were the following: gender, age group (university students: U; adolescent students: ES), performance of intensive episodes of consumption (yes/no), number of years practicing the «botellon» and grams of alcohol ingested. The results show the validity of the structure obtained for youngsters, revealing intensive episodes of alcohol consumption, as a fourgroup structure appeared in all three samples (male U, male ES, female U, female ES), in which men were always at the top of alcohol ingestion. Furthermore, ES consumed the same amount of alcohol as U, even though they had been consuming for less time. However, the youngsters who did not report intensive episodes of alcohol consumption showed some structure variability, with a tendency among women to match men"s levels of alcohol consumptio

Momento Económico (38)

Temas de hoy. 2/ De la ganaderización de la agricultura a la desganaderización actual. Retos y perspectivas Rosario Pérez Espejo. 3/ El mejoramiento en el transporte colectivo. una necesidad insoslayable Bernardo Navarro Benitez. 8/ La reconversión industrial también afecta al sector educativo Leticia Campos, 10/ México y el marco internacional de la inflación Mario J. Zepeda M. 12/ El petróleo y el conflicto del Medio Oriente Arturo Bonilla. 13

Late Holocene climate variability in the southwestern Mediterranean region: an integrated marine and terrestrial geochemical approach

A combination of marine (Alboran Sea cores, ODP 976 and TTR 300 G) and terrestrial (Zoñar Lake, Andalucia, Spain) geochemical proxies provides a high-resolution reconstruction of climate variability and human influence in the southwestern Mediterranean region for the last 4000 years at inter-centennial resolution. Proxies respond to changes in precipitation rather than temperature alone. Our combined terrestrial and marine archive documents a succession of dry and wet periods coherent with the North Atlantic climate signal. A dry period occurred prior to 2.7 cal ka BP – synchronously to the global aridity crisis of the third-millennium BC – and during the Medieval Climate Anomaly (1.4–0.7 cal ka BP). Wetter conditions prevailed from 2.7 to 1.4 cal ka BP. Hydrological signatures during the Little Ice Age are highly variable but consistent with more humidity than the Medieval Climate Anomaly. Additionally, Pb anomalies in sediments at the end of the Bronze Age suggest anthropogenic pollution earlier than the Roman Empire development in the Iberian Peninsula. The Late Holocene climate evolution of the in the study area confirms the see-saw pattern between the eastern and western Mediterranean regions and the higher influence of the North Atlantic dynamics in the western Mediterranean

Role of phosphorylation in the regulation of PRMT5

PRMT5 is a member of a group of proteins that mediate arginine methylation. It is involved in diverse cellular processes, including cell differentiation, splicing, transcription elongation and epigenetic silencing, and its expression is dysregulated in many cancers. Due to its pleiotropic functions, PRMT5 is subject to multi-level regulation. Post-translational modification (PTM) of proteins can modulate an array of cellular processes by regulating both protein interactions and protein structural changes. PRMT5 is commonly found associated with other proteins; these interactions seem to control both its catalytic activity and its substrate specificity. Recently, it became clear that PRMT5 is phosphorylated at a number of residues, which prompted us to investigate whether phosphorylation of PRMT5 regulates its subcellular localization and/or substrate choice, by facilitating phospho-dependent protein-protein interactions. To study how phosphorylation affects PRMT5 function, protein microarrays were used to identify novel phosphodependent-interacting proteins. This analysis revealed that phosphorylation mediates the interaction of PRMT5 with several SH2-domain containing proteins, 14-3-3 proteins and the FHA domain of MDC1. These novel phospho-dependent PRMT5 interactions suggest that crosstalk between kinases and arginine methyltransferases may play a pivotal role in modulating the different cellular functions of PRMT5. Additionally, we have found that the C-terminal region of PRMT5 has a recognition motif shared by PDZ domains and 14-3-3 proteins. In order to bind to this motif, 14-3-3 proteins require the C-terminus to be phosphorylated, while PDZ domain recognition is phospho-independent. From these data, a new regulatory mechanism that affects PRMT5 behavior was proposed; the action of kinases and phosphatases on PRMT5 may function as a switch to regulate interactions between 14-3-3 and PDZ domain-containing proteins. We additionally observed this paradigm with a number of proteins, suggesting that this phosphorylation dependent switch, regulating binding to 14-3-3 and PDZ domains, occurs in a wide range of protein-protein interactions. Among the recently discovered PDZ-binding partners, we have found that PRMT5 interacts with NHERF2, a membrane-associated protein that regulates the sodium ion exchanger NHE3. Through this interaction, PRMT5 is placed in close proximity to the membrane and therefore may regulate the influx of ions through selective ion channels. Overall, we hypothesize that the direct interaction of PRMT5 with selected partners mediates the appropriate localization of PRMT5, allowing it to methylate specific substrates. Physiological processes including muscle contraction, cell homeostasis and neurotransmission are controlled by the selective conduction of ions across cell membranes. Ion channels and exchangers are proteins that span cell membranes and form channels or pores, facilitating the movement of ions in and out of cells. Abnormal cellular response to the microenvironment is one of the key factors in the progression of many diseases. To study the role of the C-terminus of PRMT5 in vivo, we used CRISPR/Cas9 technology to create a mouse with the last six amino acids of PRMT5 replaced with an HA tag, as well with an altered PRMT5 C-terminus. Both mouse models lack the critical 14-3-3/PDZ binding motif. This approach revealed that the C-terminus of PRMT5 is essential for viability as no homozygous mutant embryos were recovered. Likewise, no homozygous PRMT5Δ+HA ES cell lines could be created. The results described here represent progress toward understanding PRMT5 function and regulation

Primary skin fibroblasts as a model of Parkinson's disease

Parkinson's disease is the second most frequent neurodegenerative disorder. While most cases occur sporadic mutations in a growing number of genes including Parkin (PARK2) and PINK1 (PARK6) have been associated with the disease. Different animal models and cell models like patient skin fibroblasts and recombinant cell lines can be used as model systems for Parkinson's disease. Skin fibroblasts present a system with defined mutations and the cumulative cellular damage of the patients. PINK1 and Parkin genes show relevant expression levels in human fibroblasts and since both genes participate in stress response pathways, we believe fibroblasts advantageous in order to assess, e.g. the effect of stressors. Furthermore, since a bioenergetic deficit underlies early stage Parkinson's disease, while atrophy underlies later stages, the use of primary cells seems preferable over the use of tumor cell lines. The new option to use fibroblast-derived induced pluripotent stem cells redifferentiated into dopaminergic neurons is an additional benefit. However, the use of fibroblast has also some drawbacks. We have investigated PARK6 fibroblasts and they mirror closely the respiratory alterations, the expression profiles, the mitochondrial dynamics pathology and the vulnerability to proteasomal stress that has been documented in other model systems. Fibroblasts from patients with PARK2, PARK6, idiopathic Parkinson's disease, Alzheimer's disease, and spinocerebellar ataxia type 2 demonstrated a distinct and unique mRNA expression pattern of key genes in neurodegeneration. Thus, primary skin fibroblasts are a useful Parkinson's disease model, able to serve as a complement to animal mutants, transformed cell lines and patient tissues

The karst site of Las Palomas (Guadalteba County, Málaga, Spain): A preliminary study of its MiddleeLate Pleistocene archaeopaleontological record

Cleaning works in the cave of Las Palomas in Teba (Málaga, Spain), developed by the Guadalteba Consortium, have provided a number of lithic tools and knapping products that may be ascribed to the Mode III technotypological tradition as well as remains of a number of large mammal species typical of MiddleeLate Pleistocene times. Topographic measurements help to place this ancient cave within a karst landform. This discovery opens up new perspectives in the research on the Neanderthal groups that inhabited the valleys of Guadalteba and Turón rivers in the middle basin of the Guadalhorce River, and thus in the southern region of the Iberian Peninsula

Unraveling the Complexity of Tourist Experience with NFC Technology and Mobile Wallets

By considering the tourist experience as a complex dynamic system, in this paper we depict the traveler as a kybernetes (\u3ba\u3c5\u3b2\u3b5\u3c1\u3bd\u3ae\u3c4\u3b7\u3c2 is the ancient Greek word for \u2018sea captain\u2019, \u2018steersman\u2019, or \u2018governor\u2019) in search of powerful tools to help him or her to obtain directions in the mare magnum of complexity, overcoming the fear of action and taking decisions. We focus our attention on the key role of Near Field Communication technology and mobile wallet as \u2018attenuators of complexity\u2019 in the travel and tourism industr

Evaluating Speech Enhancement Systems Through Listening Effort

Understanding degraded speech is demanding, requiring increased listening effort (LE). Evaluating processed and unprocessed speech with respect to LE can objectively indicate if speech enhancement systems benefit listeners. However, existing methods for measuring LE are complex and not widely applicable. In this study, we propose a simple method to evaluate speech intelligibility and LE simultaneously without additional strain on subjects or operators. We assess this method using results from two independent studies in Norway and Denmark, testing 76 (50+26) subjects across 9 (6+3) processing conditions. Despite differences in evaluation setups, subject recruitment, and processing systems, trends are strikingly similar, demonstrating the proposed method’s robustness and ease of implementation into existing practices.acceptedVersio

Confirmatory factor analysis of the Valencia scale on attitudes and beliefs toward hypnosis, therapist version

Health professionals' beliefs and attitudes toward hypnosis may make them reluctant to use it or even to foster misapplications and iatrogenic uses of hypnosis. The Valencia Scale on Attitudes and Beliefs toward Hypnosis-Therapist version (VSABH-T) is a specific instrument to evaluate therapists' attitudes and beliefs. The aims of this study are to evaluate the 8-factor structure of the VSABH-T proposed from a confirmatory perspective. The sample comprised 1,661 licensed psychologists who are members of the Spanish Psychological Association for the initial test and 787 for the retest. Results confirmed the 8-factor structure obtained in a previous exploratory study, namely: Fear, Memory, Help, Control, Collaboration, Interest, Magic, and Marginal. The scale also showed adequate psychometric properties, including good internal consistency and test-retest reliability

EVIDENCIAÇÃO DE INFORMAÇÕES AMBIENTAIS: PROPOSTA DE UM INDICADOR A PARTIR DA PERCEPÇÃO DE ESPECIALISTAS

This paper is aimed at listing environmental attributes, according to the degree of importance of disclosure, to compose a truly Brazilian indicator that assesses the quality of environmental information disclosure. “Environmental accounting/sustainability” experts and environmental disclosure attributes were selected, based on Brazilian and international studies. Through Delphi rounds, the experts outlined the list of the most relevant attributes to compose the indicator. The Environmental Disclosure Indicator – EDI consists of ten attributes than combine practicality and representativeness of the quality of environmental information disclosure. The results revealed a high degree of importance and, therefore, a greater weight of qualitative attributes, such as “Environmental Impacts of Products and Processes” and “Information about Residues”. The study contributes by revealing “what” should be disclosed prioritarily, that is, which are the essential environmental attributes. It contributes to verify whether the amount of environmental information companies have disclosed have necessarily meant disclosure quality. As it provides an instrument to quantify the quality of environmental disclosure, the study permits further research about the association between this disclosure and other company aspects, such as performance and corporate governance.O artigo tem por objetivo elencar atributos ambientais, conforme o grau de importância de disclosure, para composição de um indicador genuinamente brasileiro que avalie qualidade de evidenciação de informações ambientais. Selecionaram-se especialistas da área “contabilidade ambiental/sustentabilidade” e atributos de evidenciação ambiental subsidiados por estudos nacionais e internacionais. Por meio de rodadas Delphi, os especialistas delinearam o rol de atributos mais relevantes para a composição do indicador. O Indicador de Disclosure Ambiental – IDA é composto por dez atributos que combinam praticidade e representatividade da qualidade de evidenciação de informações ambientais. Os resultados revelaram alto grau de importância e, portanto, maior peso para atributos qualitativos, como “Impactos Ambientais de Produtos e Processos” e “Informações sobre Resíduos”. A contribuição do estudo consiste em revelar “o que” se deve evidenciar prioritariamente, ou seja, quais são os atributos ambientais essenciais. Contribui para que verifique se a quantidade de informações ambientais evidenciadas pelas companhias tem significado necessariamente qualidade de disclosure. Por fornecer um instrumento para quantificação da qualidade do disclosure ambiental, o estudo possibilita pesquisas posteriores sobre a associação entre esta evidenciação e demais aspectos das companhias, como desempenho e governança corporativ