Ontology-based data access with databases: a short course

Ontology-based data access (OBDA) is regarded as a key ingredient of the new generation of information systems. In the OBDA paradigm, an ontology defines a high-level global schema of (already existing) data sources and provides a vocabulary for user queries. An OBDA system rewrites such queries and ontologies into the vocabulary of the data sources and then delegates the actual query evaluation to a suitable query answering system such as a relational database management system or a datalog engine. In this chapter, we mainly focus on OBDA with the ontology language OWL 2QL, one of the three profiles of the W3C standard Web Ontology Language OWL 2, and relational databases, although other possible languages will also be discussed. We consider different types of conjunctive query rewriting and their succinctness, different architectures of OBDA systems, and give an overview of the OBDA system Ontop

The effects of aging on neuropil structure in mouse somatosensory cortex-A 3D electron microscopy analysis of layer 1

This study has used dense reconstructions from serial EM images to compare the neuropil ultrastructure and connectivity of aged and adult mice. The analysis used models of axons, dendrites, and their synaptic connections, reconstructed from volumes of neuropil imaged in layer 1 of the somatosensory cortex. This shows the changes to neuropil structure that accompany a general loss of synapses in a well-defined brain region. The loss of excitatory synapses was balanced by an increase in their size such that the total amount of synaptic surface, per unit length of axon, and per unit volume of neuropil, stayed the same. There was also a greater reduction of inhibitory synapses than excitatory, particularly those found on dendritic spines, resulting in an increase in the excitatory/inhibitory balance. The close correlations, that exist in young and adult neurons, between spine volume, bouton volume, synaptic size, and docked vesicle numbers are all preserved during aging. These comparisons display features that indicate a reduced plasticity of cortical circuits, with fewer, more transient, connections, but nevertheless an enhancement of the remaining connectivity that compensates for a generalized synapse loss

Impact of dynamical charm quarks

We compute and compare the continuum limits of several quantities in QCD with and without a dynamical charm quark. We consider both low energy quantities, like the hadronic scales r0 and t 0 , and high energy quantities, like the charmonium masses

GRP78 Mediates Cell Growth and Invasiveness in Endometrial Cancer.

Abstract Recent studies have indicated that endoplasmic reticulum stress, the unfolded protein response activation and altered GRP78 expression can play an important role in a variety of tumors development and progression. Very recently we reported for the first time that GRP78 is increased in endometrial tumors. However, whether GRP78 could play a role in the growth and/or invasiveness of endometrial cancer cells is still unknown. Here we report that the silencing of GRP78 expression affects both cell growth and invasiveness of Ishikawa and AN3CA cells, analyzed by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and transwell migration assay, respectively. At variance with Ishikawa cells, AN3CA cells showed, besides an endoplasmic reticulum, also a plasma membrane GRP78 localization, evidenced by both immunofluorescence and cell membrane biotinylation experiments. Intriguingly, flow cytometry experiments showed that the treatment with a specific antibody targeting GRP78 C-terminal domain caused apoptosis in AN3CA but not in Ishikawa cells. Induction of apoptosis in AN3CA cells was not mediated by the p53 pathway activation but was rather associated to reduced AKT phosphorylation. Interestingly, immunofluorescence analysis evidenced that endometrioid adenocarcinoma tissues displayed, similarly to AN3CA cells, also a GRP78 plasma membrane localization. These data suggest that GRP78 and its plasma membrane localization, might play a role in endometrial cancer development and progression and might constitute a novel target for the treatment of endometrial cancer

Exsolution of catalytically active iridium nanoparticles from strontium titanate

The search for new functional materials that combine high stability and efficiency with reasonable cost and ease of synthesis is critical for their use in renewable energy applications. Specifically in catalysis, nanoparticles, with their high surface-to-volume ratio, can overcome the cost implications associated with otherwise having to use large amounts of noble metals. However, commercialized materials, that is, catalytic nanoparticles deposited on oxide supports, often suffer from loss of activity because of coarsening and carbon deposition during operation. Exsolution has proven to be an interesting strategy to overcome such issues. Here, the controlled emergence, or exsolution, of faceted iridium nanoparticles from a doped SrTiO3 perovskite is reported and their growth preliminary probed by in situ electron microscopy. Upon reduction of SrIr0.005Ti0.995O3, the generated nanoparticles show embedding into the oxide support, therefore preventing agglomeration and subsequent catalyst degradation. The advantages of this approach are the extremely low noble metal amount employed (∼0.5% weight) and the catalytic activity reported during CO oxidation tests, where the performance of the exsolved SrIr0.005Ti0.995O3 is compared to the activity of a commercial catalyst with 1% loading (1% Ir/Al2O3). The high activity obtained with such low doping shows the possibility of scaling up this new catalyst, reducing the high cost associated with iridium-based materials.PostprintPostprintPeer reviewe

Minimizing material consumption of 3d printing with stress-guided optimization

3D printing has been widely used in daily life, industry, architecture, aerospace, crafts, art, etc. Minimizing 3D printing material consumption can greatly reduce the costs. Therefore, how to design 3D printed objects with less materials while maintain structural soundness is an important problem. The current treatment is to use thin shells. However, thin shells have low strength. In this paper, we use stiffeners to stiffen 3D thin-shell objects for increasing the strength of the objects and propose a stress guided optimization framework to achieve minimum material consumption. First, we carry out finite element calculations to determine stress distribution in 3D objects and use the stress distribution to guide random generation of some points called seeds. Then we map the 3D objects and seeds to a 2D space and create a Voronoi Diagram from the seeds. The stiffeners are taken to be the edges of the Voronoi Diagram whose intersections with the edges of each of the triangles used to represent the polygon models of the 3D objects are used to define stiffeners. The obtained intersections are mapped back to 3D polygon models and the cross-section size of stiffeners is minimized under the constraint of the required strength. Monte-Carlo simulation is finally introduced to repeat the process from random seed generation to cross-section size optimization of stiffeners. Many experiments are presented to demonstrate the proposed framework and its advantages

Retinoic acid receptor activation reprograms senescence response and enhances anti-tumor activity of natural killer cells

Cellular senescence can exert dual effects in tumors, either suppressing or promoting tumor progression. The senescence-associated secretory phenotype (SASP), released by senescent cells, plays a crucial role in this dichotomy. Consequently, the clinical challenge lies in developing therapies that safely enhance senescence in cancer, favoring tumor-suppressive SASP factors over tumor-promoting ones. Here, we identify the retinoic-acid-receptor (RAR) agonist adapalene as an effective pro-senescence compound in prostate cancer (PCa). Reactivation of RARs triggers a robust senescence response and a tumor-suppressive SASP. In preclinical mouse models of PCa, the combination of adapalene and docetaxel promotes a tumor-suppressive SASP that enhances natural killer (NK) cell-mediated tumor clearance more effectively than either agent alone. This approach increases the efficacy of the allogenic infusion of human NK cells in mice injected with human PCa cells, suggesting an alternative therapeutic strategy to stimulate the anti-tumor immune response in "immunologically cold" tumors