Inactivation of the Na,K-ATPase by radiation-induced free radicals Evidence for a radical-chain mechanism

AbstractFree radicals produced by water radiolysis were used to study the inactivation of the enzymatic activity of the Na,K-ATPase. A decrease of the activity to virtually zero with a mono-exponential dependence on the radiation dose was observed. The inactivation process is initiated by hydroxyl radicals. This was shown by the effect of appropriate radical scavengers such as t-butanol, formate and vitamin C. In all cases a significant increase in the characteristic D37 dose of inactivation was observed. Inactivation was found to show a so-called inverse dose-rate effect, i.e, the sensitivity of the enzyme to radical attack is increased if the dose rate is reduced. The data were found to agree with the relationship 1/D371̃/D1/2, which is known to be a strong indicator of a radical chain mechanism. This means that the inactivation, after initiation by single radicals, is amplified by a subsequent chain mechanism

Noble gases from the interstellar medium trapped on the MIR space station and analyzed by in vacuo etching

Introduction: The composition of the present interstellar medium (ISM) provides an important benchmark in cosmochemistry. It serves as a reference for galactic chemical evolution (GCE) models, solar mixing predictions and provides information for understanding Big Bang nucleosynthesis. The present-day ISM 3He abundance allows, combined with the protosolar 3He, deduced from the Jovian atmosphere or meteorites [1,2], tracing the GCE over the past 4.56 Ga. 3He/4He = (2.5 0.6) x 10-4 has been determined for the local ISM [3]. However, the uncertainty is too large to better constrain GCE models and - in combination with the present-day solar wind value - the protosolar D/H [4]

A numerical investigation on the vortex formation and flow separation of the oscillatory flow in jet pumps

A two-dimensional computational fluid dynamics model is used to predict the oscillatory flow through a tapered cylindrical tube section (jet pump) placed in a larger outer tube. Due to the shape of the jet pump, there will exist an asymmetry in the hydrodynamic end effects which will cause a time-averaged pressure drop to occur that can be used to cancel Gedeon streaming in a closed-loop thermoacoustic device. The performance of two jet pump geometries with different taper angles is investigated. A specific time-domain impedance boundary condition is implemented in order to simulate traveling acoustic wave conditions. It is shown that by scaling the acoustic displacement amplitude to the jet pump dimensions, similar minor losses are observed independent of the jet pump geometry. Four different flow regimes are distinguished and the observed flow phenomena are related to the jet pump performance. The simulated jet pump performance is compared to an existing quasi-steady approximation which is shown to only be valid for small displacement amplitudes compared to the jet pump length.Comment: The following article has been accepted by the Journal of the Acoustical Society of America. After it is published, it will be found at: http://scitation.aip.org/JAS

Jet pumps for thermoacoustic applications: design guidelines based on a numerical parameter study

The oscillatory flow through tapered cylindrical tube sections (jet pumps) is characterized by a numerical parameter study. The shape of a jet pump results in asymmetric hydrodynamic end effects which cause a time-averaged pressure drop to occur under oscillatory flow conditions. Hence, jet pumps are used as streaming suppressors in closed-loop thermoacoustic devices. A two-dimensional axisymmetric computational fluid dynamics model is used to calculate the performance of a large number of conical jet pump geometries in terms of time-averaged pressure drop and acoustic power dissipation. The investigated geometrical parameters include the jet pump length, taper angle, waist diameter and waist curvature. In correspondence with previous work, four flow regimes are observed which characterize the jet pump performance and dimensionless parameters are introduced to scale the performance of the various jet pump geometries. The simulation results are compared to an existing quasi-steady theory and it is shown that this theory is only applicable in a small operation region. Based on the scaling parameters, an optimum operation region is defined and design guidelines are proposed which can be directly used for future jet pump design.Comment: The following article has been accepted by the Journal of the Acoustical Society of America. After it is published, it will be found at http://scitation.aip.org/JAS

Majorana modes and p-wave superfluids for fermionic atoms in optical lattices.

The quest for realization of non-Abelian phases of matter, driven by their possible use in fault-tolerant topological quantum computing, has been spearheaded by recent developments in p-wave superconductors. The chiral p(x)+ip(y)-wave superconductor in two-dimensions exhibiting Majorana modes provides the simplest phase supporting non-Abelian quasiparticles and can be seen as the blueprint of fractional topological order. Alternatively, Kitaev's Majorana wire has emerged as an ideal toy model to understand Majorana modes. Here we present a way to make the transition from Kitaev's Majorana wires to two-dimensional p-wave superconductors in a system with cold atomic gases in an optical lattice. The main idea is based on an approach to generate p-wave interactions by coupling orbital degrees of freedom with strong s-wave interactions. We demonstrate how this design can induce Majorana modes at edge dislocations in the optical lattice, and we provide an experimentally feasible protocol for the observation of the non-Abelian statistics.We acknowledge support by the Center for Integrated Quantum Science and Technology (IQST) and the Deutsche Forschungsgemeinschaft (DFG) within SFB TRR 21, the Leverhulme Trust (ECF-2011-565), the Newton Trust of the University of Cambridge, the Royal Society (UF120157), SFB FoQus (FWF Project No. F4006-N16), the ERC Synergy Grant UQUAM, SIQS, and Swiss National Science Foundation. GM, SH, CK, and HB thank the Institut d’Etudes Scientifiques Cargèse and CECAM for their hospitality.This is the accepted manuscript of an article published in Nature Communications [A Bühler, N Lang, CV Kraus, G Möller, SD Huber, HP Büchler Nature Communications 5:4504 doi: 10.1038/ncomms5504 (2014)]

Effects of Orthogonal Rotating Electric Fields on Electrospinning Process

Electrospinning is a nanotechnology process whereby an external electric field is used to accelerate and stretch a charged polymer jet, so as to produce fibers with nanoscale diameters. In quest of a further reduction in the cross section of electrified jets hence of a better control on the morphology of the resulting electrospun fibers, we explore the effects of an external rotating electric field orthogonal to the jet direction. Through extensive particle simulations, it is shown that by a proper tuning of the electric field amplitude and frequency, a reduction of up to a $30 \%$ in the aforementioned radius can be obtained, thereby opening new perspectives in the design of future ultra-thin electrospun fibres. Applications can be envisaged in the fields of nanophotonic components as well as for designing new and improved filtration materials.Comment: 22 pages, 8 figure

Thermodynamic Properties of the Dimerised and Frustrated S=1/2 Chain

By high temperature series expansion, exact diagonalisation and temperature density-matrix renormalisation the magnetic susceptibility $\chi(T)$ and the specific heat $C(T)$ of dimerised and frustrated $S=1/2$ chains are computed. All three methods yield reliable results, in particular for not too small temperatures or not too small gaps. The series expansion results are provided in the form of polynomials allowing very fast and convenient fits in data analysis using algebraic programmes. We discuss the difficulty to extract more than two coupling constants from the temperature dependence of $\chi(T)$.Comment: 14 pages, 13 figures, 4 table

Global Grid User Support Building a worldwide distributed user support infrastructure

The organisation and management of the user support in a global escience computing infrastructure such as EGEE (Enabling Grids for E-sciencE), a series of EU projects, is one of the challenges of the Grid. Given the widely distributed nature of the organisation, and the spread of expertise for installing, configuring, managing and troubleshooting the Grid middleware services, a standard centralised model could not be deployed in EGEE. This paper presents the model used in EGEE for building a reliable infrastructure for user, virtual organisation and operations support. A short overview of EGEE is given. The model for supporting a production quality infrastructure for scientific applications will be described in detail. The advantages of the chosen model will be presented and the possible difficulties will be discussed. In this paper we will also describe a scheme of how knowledge management can be used in Grid user support and first steps towards a realisation in the framework of the EGEE user support infrastructure

Vaccination recommendations for adult patients with autoimmune inflammatory rheumatic diseases

BACKGROUND: The number of individuals with autoimmune inflammatory rheumatic diseases (AIIRDs) treated with immunosuppressive drugs is increasing steadily. The variety of immunosuppressive drugs and, in particular, biological therapies is also rising. The immunosuppressants, as well as the AIIRD itself, increase the risk of infection in this population. Thus, preventing infections by means of vaccination is of utmost importance. New Swiss vaccination recommendations for AIIRD patients were initiated by the Swiss Federal Office of Public Health and prepared by a working group of the Federal Commission for Vaccination Issues as well as by consultation of international experts. METHODS: A literature search was performed in electronic databases (Cochrane, Medline, PubMed, Embase). In addition, unpublished literature was identified through a targeted website search of relevant organisations and international conferences dealing with vaccination, infectious diseases and rheumatology. RESULTS: Although data are scarce, the following main points were retrieved from the literature. Inactivated vaccines are safe, but their immunogenicity may be reduced in AIIRD patients, especially if they are under immunosuppressive therapy. Rituximab and abatacept appear to reduce significantly immune responses after vaccination. Live vaccines are generally contraindicated under immunosuppressive therapy owing to safety concerns. Specific exceptions, as well as time intervals for the administration of live vaccines after interruption of an immunosuppressive therapy, have been formulated in this article. CONCLUSION: More evidence regarding the immunogenicity and safety of vaccinations in AIIRD patients under various therapies is needed. Vaccination recommendations should be updated on a regular basis, as more scientific data will become available