48 research outputs found
Study on effects of extracted probiotics from microbial flora of digestive system on growth indices in beluga (Huso huso) and Persian sturgeon (Acipenser persicus)
Isolation of Lactic Acid Bacteria (LAB) was carried out from gastro intestinal
tract of beluga and Persian sturgeon at international sturgeon research institute
and PCR has been used for bacteria Identification.
Two species of LAB including Enterococcus seriolicida and Leuconostoc
mesenteroides were isolated from Gastrointestinal tract (GI) of persian sturgeon
in this study and the counts of Leu. mesenteroides (4.63×102 CFU/gr of GI)
was significantly higher than other species. Lactobacillus curvatus,
Lactococcus raffinolactis, Lactococcus lactis and Streptococcus sp. were also
isolated from GI of beluga and maximum counts was belonged to Lb. curvatus
(4.63×102 CFU/gr of GI) in this species. Dominant species were lyophilized
and adding to the water since start of mix feeding of sturgeon with different
counts including 2×109, 5×109 and 9×109 CFU/gr of live food, 4 times a day.
The results revealed that the maximum and minimum growth rate and protease,
amylase, and lipase activity in beluga was gained by using of Lb. curvatus with
total viable count of 9×10 9 CFU/gr of live food and Leu. mesenteroides with
total viable count of 9×109 CFU/gr of live food.
According to the results of this study, the maximum and minimum growth rate
and protease, amylase, and lipase activity in Persian sturgeon was gained by
using of Leu. mesenteroides with total viable count of 2×10 9 CFU/gr of live
food and Lb. curvatus with total viable count of 9×109 CFU/gr of live food.
Histological study showed that gastrointestinal development was same during
larva rearing in control and other treatments but the size of liver was bigger in
treatments that received nonspecific LAB in both species.
According to the results, positive effects of using dominant specific LAB
bacteria for larviculture of sturgeon has been proved in this study
Factors influencing nurses' acceptance of hospital information systems in Iran: application of the Unified Theory of Acceptance and Use of Technology
Abstract User acceptance is a precondition for successful implementation of hospital information systems (HISs). Increasing investment in information technology by healthcare organisations internationally has made user acceptance an important issue in technology implementation and management. Despite the increased focus on hospital information systems, there continues to be user resistance. The present study aimed to investigate the factors affecting hospital information systems nurse-user acceptance of HISs, based on the Unified Theory of Acceptance and Use of Technology (UTAUT), in the Shiraz University of Medical Sciences teaching hospitals. A descriptive-analytical research design was employed to study nurses' adoption and use of HISs. Data collection was undertaken using a cross-sectional survey of nurses (n=303). The research model was examined using the LISREL path confirmatory modeling. The results demonstrated that the nurses' behavioural intention (BI) to use hospital information systems was predicted by Performance Expectancy (PE) (β= 2.34, p<0.01), Effort Expectancy (EE) (β= 2.21, p<0.01), Social Influence (SI) (β= 2.63, p<0.01) and Facilitating Conditions (FC) (β= 2.84, p<0.01). The effects of these antecedents of BI explained 72.8% of the variance in nurses' intention to use hospital information systems (R 2 = 0.728). Application of the research model suggested that nurses' acceptance of HISs was influenced by performance expectancy, effort expectancy, social influence and facilitating conditions, with performance expectancy having the strongest effect on user intention
Transcriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small RNA-cargo
Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction.
Introductio
Bacterial membrane-derived vesicles attenuate vancomycin activity against methicillin-resistant staphylococcus aureus
Methicillin-resistant Staphylococcus aureus (MRSA) has evolved numerous antimicrobial resistance mechanisms and is identified as a serious public health threat by the World Health Organization and U.S. Centers for Disease Control and Prevention. The glycopeptide vancomycin (VAN) remains a cornerstone of therapy for severe MRSA infections despite increasing reports of therapeutic failure in hospitalized patients with bacteremia or pneumonia. Recently, the role of released bacterial-derived membrane vesicles (MVs) in antibiotic resistance has garnered attention. Here we examined the effect of exogenous MRSA-derived MVs on VAN activity against MRSA in vitro, using minimum inhibitory concentration and checkerboard assays, and ex vivo, incorporating components of host innate immunity such as neutrophils and serum complement present in blood. Additionally, the proteome of MVs from VAN-exposed MRSA was characterized to determine if protein expression was altered. The presence of MVs increased the VAN MIC against MRSA to values where clinical failure is commonly observed. Furthermore, the presence of MVs increased survival of MRSA pre-treated with sub-MIC concentrations of VAN in whole blood and upon exposure to human neutrophils but not human serum. Unbiased proteomic analysis also showed an elevated expression of MV proteins associated with antibiotic resistance (e.g., marR) or proteins that are functionally linked to cell membrane/wall metabolism. Together, our findings indicate MRSA-derived MVs are capable of lowering susceptibility of the pathogen to VAN, whole-blood- and neutrophil-mediated killing, a new pharmacodynamic consideration for a drug increasingly linked to clinical treatment failures
Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis
publishedVersio
Immunization with lytic polysaccharide monooxygenase CbpD induces protective immunity against Pseudomonas aeruginosa pneumonia
publishedVersio