Ajayi, Clement

Askarian, Fatemeh

Diep, Dzung Bao

Hanssen, Anne-Merethe

Johannessen, Mona

Pettersen, ingvild

Sollid, Johanna U Ericson

Van Sorge, Nina M.

Scientific Reports

PubMed

HSN Open Archive

The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells

Staphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors 
involved in colonization are adhesins such as the microbial surface components recognizing adhesive 
matrix molecules (MSCRAMMs). Serine aspartate repeat containing protein D (SdrD) is involved in 
adhesion to human squamous cells isolated from the nose. Here, we identify Desmoglein 1 (Dsg1) 
as a novel interaction partner for SdrD. Genetic deletion of 
sdrD in S. aureusNCTC8325-4 through 
allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells 
in vitro. 
Complementary gain-of-function was demonstrated by heterologous expression of SdrD in Lactococcus 
lactis, which increased adherence to HaCaT cells. Also ectopic expression of Dsg1 in HEK293 cells 
resulted in increased adherence of S. aureus
 NCTC8325-4 in vitro. Increased adherence of NCTC8325-4, compared to NCTC8325-4ΔsdrD,
to the recombinant immobilized Dsg1 demonstrated direct interaction between SdrD and Dsg1. Specificity of SdrD interaction with Dsg1 was further verified using flow 
cytometry and confirmed binding of recombinant SdrD to HaCaT cells expressing Dsg1 on their surface. 
These data demonstrate that Dsg1 is a host ligand for SdrD

Pettersen, Ingvild

Sollid, Johanna U. Ericson

NORA - Norwegian Open Research Archives

Source at http://doi.org/10.1038/srep22134Staphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors 
involved in colonization are adhesins such as the microbial surface components recognizing adhesive 
matrix molecules (MSCRAMMs). Serine aspartate repeat containing protein D (SdrD) is involved in 
adhesion to human squamous cells isolated from the nose. Here, we identify Desmoglein 1 (Dsg1) 
as a novel interaction partner for SdrD. Genetic deletion of 
sdrD in S. aureusNCTC8325-4 through 
allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells 
in vitro. 
Complementary gain-of-function was demonstrated by heterologous expression of SdrD in Lactococcus 
lactis, which increased adherence to HaCaT cells. Also ectopic expression of Dsg1 in HEK293 cells 
resulted in increased adherence of S. aureus
 NCTC8325-4 in vitro. Increased adherence of NCTC8325-4, compared to NCTC8325-4ΔsdrD,
to the recombinant immobilized Dsg1 demonstrated direct interaction between SdrD and Dsg1. Specificity of SdrD interaction with Dsg1 was further verified using flow 
cytometry and confirmed binding of recombinant SdrD to HaCaT cells expressing Dsg1 on their surface. 
These data demonstrate that Dsg1 is a host ligand for SdrD

Munin - Open Research Archive

English

Brage NMBU

The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells

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Brage NMBU