Beikrautsamenvorrat im Vergleich reduzierter und konventioneller Bodenbearbeitung unter Biobedingungen

Armengot L, Berner A, Blanco-Moreno J, Mäder P & Sans FX (2014) Long-term feasibility of reduced tillage in organic farming. Agron Sustain Dev 35(1): 339-346. Cooper J et al. (2016) Shallow non-inversion tillage in organic farming maintains crop yields and increases soil C stocks: a meta-analysis. Agron Sustain Dev 36: 1-20. Gruber S & Claupein W (2009) Effect of tillage intensity on weed infestation in organic farming. Soil and Tillage Research 105(1): 104-111. Montgomery DR (2007) Dirt: The Erosion of Civilizations. University of California Press, California. Moonen AC & Barberi P (2004) Size and composition of the weed seedbank after 7 years of different cover-crop-maize management systems. Weed Research 44(3): 163-177

Securing the food system together: Innovation development with the farmer, for the farmer by the farmer

In recent years, the limitations of top-down approaches for development-oriented research have become apparent. Therefore, scientists have started to explore alternatives such as participatory research. By involving many stakeholders throughout the different stages of innovation development, participatory research helps tackling the multi-faceted challenges the global food system is facing today. Since almost a decade, the Research Institute of Organic Agriculture (FiBL) has been running a large program called “Farming Systems Comparison in the Tropics” (SysCom) in Bolivia, India and Kenya. Besides maintaining four Long-Term farming systems comparison Experiments (LTE), Participatory On-farm Research (POR) is a strong component of SysCom. In POR, we aim at fostering sustainable agriculture by developing locally adapted solutions for specific challenges of farmers. Researchers, extension agents, private sector and farmers work together from the stage of problem identification to the achievement of results, and finally scaling them. By taking into account local knowledge and available resources, significant achievements have been made in all of the three countries. In India, we developed a new kind of phosphorous fertilizer for use in organic farming with which farmers substantially increased the yields of their main rotation crops across different types of soils and farms. We also standardized the preparation of homemade organic pesticides from different plants. In addition, we developed a practicable guide for pest monitoring in the small scale farmers’ context. In Bolivia, we tested different cocoa varieties. Results showed that some local selections were not only among the most productive, but also presented the earliest maturation and some degree of resistance to the frosty pod rot disease (Moniliophthora roreri). In Kenya, we addressed the lack of organic materials for soil fertility management. By testing different local residues and manures, as well as composting techniques, we brought forward a productivity innovation for local small scale farmers. On a local level, the results were made available in the form of leaflets, brochures and video clips. These were used by farmers, extension workers and other stakeholders. Combining applied science (LTE) with participatory action research (POR) is a powerful approach which has proven successful in SysCom. The beauty of this approach lies in the fact that it allows for multiple perceptions. However, it also comes with its own challenges which mainly lie in the fact that it requires different mindsets than traditional research approaches, as the whole process is relatively less controlled by researchers. If we teach and assist farmers to carry out research on their own farms, it will be easier for them to appreciate the effects of novel technologies which, in turn, will enhance their adoption

Can organic agriculture contribute to sustainable development in the tropics?

Agricultural intensification over last decades has resulted in a great increase of crop yields, but it also had a detrimental impact on biodiversity. The dramatic decline of arable weed diversity is a matter of great concern because weeds have an important ecological function as a key component of the food web of agroecosystems. Weeds are suitable indicators of management effects on wildlife diversity in arable crops because they have high sensitivity to cultivation measures and have a strong relation to other organism groups. Nevertheless, the effect of farming management on weed abundance and diversity will be more reliable on weed seed bank rather than on aboveground weed community because it is the result of processes that have occurred in the past and consequently, it could better reflect the effect of the agricultural practices over the years

Molecular and Clinical Predictors of Quality of Life in Chronic Rhinosinusitis with Nasal Polyps

Factors influencing the quality of life (QoL) of patients with chronic rhinosinusitis with nasal polyposis (CRSwNP) are poorly understood. We set out to determine the predictive factors on patients' QoL using the Sino-Nasal Outcome Test-22 (SNOT-22); (2) Methods: An ambispective analysis of data from patients diagnosed with CRSwNP in our institution. All the patients underwent a nasal polyp biopsy and completed the SNOT-22 questionnaire. Demographic and molecular data as well as the SNOT-22 scores were collected. Patients were classified in six subgroups considering the presence of asthma, non-steroidal drugs (NSAID) intolerance and corticosteroid resistance; (3) Results: The mean SNOT-22 score was 39. Considering the clinical parameters, the SNOT-22 value was significantly associated with NSAID intolerance (p = 0.04) and the endoscopic polyp score (p = 0.04). A high SNOT-22 value was also correlated with high tissue eosinophilia (p = 0.01) and high IL-8 expression; (4) Conclusions: Eosinophilia, IL-8 expression and NSAID intolerance can be used as predictors of worse QoL in patients with CRSwNP

Checklist for the Multidisciplinary Approach to United Airway in Patients with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) and asthma

Dear Editor, The united airways concept calls for a multidisciplinary approach to asthma and/or chronic rhinitis/rhinosinusitis (CRS), aimed at integral airway treatment1, 2 and better coordination among specialists.3 Failure to treat rhinitis/rhinosinusitis is associated with poor asthma control, especially of severe asthma.4, 5 Biological treatments targeting type 2 (T2) inflammatory mediators in severe respiratory diseases offer a new therapeutic option directed against the pathophysiological mechanism of these difficult-to-control united airways diseases (UAD)..

Evaluation of Mucociliary Clearance by Three Dimension Micro-CT-SPECT in Guinea Pig: Role of Bitter Taste Agonists

Different image techniques have been used to analyze mucociliary clearance (MCC) in humans, but current small animal MCC analysis using in vivo imaging has not been well defined. Bitter taste receptor (T2R) agonists increase ciliary beat frequency (CBF) and cause bronchodilation but their effects in vivo are not well understood. This work analyzes in vivo nasal and bronchial MCC in guinea pig animals using three dimension (3D) micro-CT-SPECT images and evaluates the effect of T2R agonists. Intranasal macroaggreggates of albumin-Technetium 99 metastable (MAA-Tc99m) and lung nebulized Tc99m albumin nanocolloids were used to analyze the effect of T2R agonists on nasal and bronchial MCC respectively, using 3D micro-CT-SPECT in guinea pig. MAA-Tc99m showed a nasal mucociliary transport rate of 0.36 mm/min that was increased in presence of T2R agonist to 0.66 mm/min. Tc99m albumin nanocolloids were homogeneously distributed in the lung of guinea pig and cleared with time-dependence through the bronchi and trachea of guinea pig. T2R agonist increased bronchial MCC of Tc99m albumin nanocolloids. T2R agonists increased CBF in human nasal ciliated cells in vitro and induced bronchodilation in human bronchi ex vivo. In summary, T2R agonists increase MCC in vivo as assessed by 3D micro-CT-SPECT analysis

Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism

Plants are able to orient their growth according to gravity, which ultimately controls both shoot and root architecture.1 Gravitropism is a dynamic process whereby gravistimulation induces the asymmetric distribution of the plant hormone auxin, leading to asymmetric growth, organ bending, and subsequent reset of auxin distribution back to the original pre-gravistimulation situation.1, 2, 3 Differential auxin accumulation during the gravitropic response depends on the activity of polarly localized PIN-FORMED (PIN) auxin-efflux carriers.1, 2, 3, 4 In particular, the timing of this dynamic response is regulated by PIN2,5,6 but the underlying molecular mechanisms are poorly understood. Here, we show that MEMBRANE ASSOCIATED KINASE REGULATOR2 (MAKR2) controls the pace of the root gravitropic response. We found that MAKR2 is required for the PIN2 asymmetry during gravitropism by acting as a negative regulator of the cell-surface signaling mediated by the receptor-like kinase TRANSMEMBRANE KINASE1 (TMK1).2,7, 8, 9, 10 Furthermore, we show that the MAKR2 inhibitory effect on TMK1 signaling is antagonized by auxin itself, which triggers rapid MAKR2 membrane dissociation in a TMK1-dependent manner. Our findings suggest that the timing of the root gravitropic response is orchestrated by the reversible inhibition of the TMK1 signaling pathway at the cell surface

Methodology for an effective risk assessment of urban areas: progress and first results of the merisur project

The progress and results the MERISUR, Methodology for an Effective RISk assessment of URban areas, are presented. This project aims at developing an effective methodology for urban seismic risk assessment that provides solutions to some deficiencies detected after recent damaging events worldwide, including risk mitigation actions based on benefit/cost ratios. In a fisrt stage, the hazard and vulnerability models are developed and improved. A procedure to determine the hazard-controlling seismogenic fault, contsistent with different probability levels, is established. Methods to include active faults as individual sources and to consider near filed effects that significantly amplify ground motions are proposed. A more complete description of seismic vulnerability encompassing structural, non-structural components is accomplished. Vulnerability modifiers to incorporate effects or urban parameters on vulnerability classes are also quantified. A distinction is also made between damage to structural and non-structural building elements. For this purpose, a pushover analysis is specifically carried out to model building response and damage trends on non-structural elements. This gives the primary damage. In addition, the area covered by the resulting debris is also estimated both in inner spaces (within the building) and in the outer space (public roads and streets). In this way, a volume of debris will be associated to each area unit of the city, and the potential damage to persons and elements exposed, such as urban furniture and vehicles, will be assessed. This constitutes the secondary damage. A static level of occupation (building, urban furniture, etc.) and a dynamic level of occupation (persons, vehicles) will be assigned to each area unit of the city, hereby defining the exposure in time and space. Earthquake losses related to primary damage of building components and to secondary damage (such as urban furniture and vehicles) will be also assessed. Cost/benefit ratios between ex ante risk mitigation measurements will be developed in order to decide whether risk transfer or risk retention is preferable for different risk scenarios. This analysis will confer effectiveness to the results of a seismic risk study. Overall, the estimate of earthquake losses and cost/benefit ratios are topics with little presence in the scientific literature concerning damaging earthquakes in Spain. Thus, the results of this study will provide effective solutions to the challenge to society tackled in this proposal

Study of USH1 Splicing Variants through Minigenes and Transcript Analysis from Nasal Epithelial Cells

Usher syndrome type I (USH1) is an autosomal recessive disorder characterized by congenital profound deafness, vestibular areflexia and prepubertal retinitis pigmentosa. The first purpose of this study was to determine the pathologic nature of eighteen USH1 putative splicing variants found in our series and their effect in the splicing process by minigene assays. These variants were selected according to bioinformatic analysis. The second aim was to analyze the USH1 transcripts, obtained from nasal epithelial cells samples of our patients, in order to corroborate the observed effect of mutations by minigenes in patient’s tissues. The last objective was to evaluate the nasal ciliary beat frequency in patients with USH1 and compare it with control subjects. In silico analysis were performed using four bioinformatic programs: NNSplice, Human Splicing Finder, NetGene2 and Spliceview. Afterward, minigenes based on the pSPL3 vector were used to investigate the implication of selected changes in the mRNA processing. To observe the effect of mutations in the patient’s tissues, RNA was extracted from nasal epithelial cells and RT-PCR analyses were performed. Four MYO7A (c.470G>A, c.1342_1343delAG, c.5856G>A and c.3652G>A), three CDH23 (c.2289+1G>A, c.6049G>A and c.8722+1delG) and one PCDH15 (c.3717+2dupTT) variants were observed to affect the splicing process by minigene assays and/or transcripts analysis obtained from nasal cells. Based on our results, minigenes are a good approach to determine the implication of identified variants in the mRNA processing, and the analysis of RNA obtained from nasal epithelial cells is an alternative method to discriminate neutral Usher variants from those with a pathogenic effect on the splicing process. In addition, we could observe that the nasal ciliated epithelium of USH1 patients shows a lower ciliary beat frequency than control subjects

Franchisors' disclosure duty: market transparency and franchisee protection

The franchisee is usually the most vulnerable part of the franchise relationship, and should therefore receive greater protection from the legal framework. In this regard, the franchisor's pre-contractual disclosure duty has evolved in its legal status. Whereas its original purpose was to ensure transparency in the market, it now serves to protect the franchisee. In this paper, we compare the franchisor's obligations established by the legal framework in Spain with those set out in the Model Law drawn up by The International Institute for the Unification of Private Law.Lapiedra-Alcami, R.; Reig Fabado, I.; Rueda Armengot, C. (2014). Franchisors' disclosure duty: market transparency and franchisee protection. Service Industries Journal. 34(9-10):788-795. doi:10.1080/02642069.2014.905917S788795349-10Chaudey, M., & Fadairo, M. (2010). Contractual design and networks performance: empirical evidence from franchising. Applied Economics, 42(4), 529-533. doi:10.1080/00036840701704428Collins, H. (2008). Principles, Definitions and Model Rules of European Private Law: Draft Common Frame of Reference (DCFR) Interim Outline Edition, prepared by the Study Group on a European Civil Code and the Research group on EC Private Law (Acquis group) by Christian von Bar, Eric Clive, Hans Schulte-Nöcke (eds). Modern Law Review, 71(5), 840-844. doi:10.1111/j.1468-2230.2008.00718.xPeris-Ortiz, M., Peris Bonet, F. J., & Rueda-Armengot, C. (2011). Vertical integration in production and services: development in transaction cost economics. Service Business, 5(1), 87-97. doi:10.1007/s11628-011-0103-0Perryman, A. A., & Combs, J. G. (2011). Who should own it? An agency-based explanation for multi-outlet ownership and co-location in plural form franchising. Strategic Management Journal, 33(4), 368-386. doi:10.1002/smj.1947Rondan-Cataluña, F. J., Navarro-Garcia, A., Diez-De Castro, E. C., & Rodriguez-Rad, C. J. (2012). Reasons for the expansion in franchising: is it all said? The Service Industries Journal, 32(6), 861-882. doi:10.1080/02642069.2010.550041Shane, S. (2001). Organizational Incentives and Organizational Mortality. Organization Science, 12(2), 136-160. doi:10.1287/orsc.12.2.136.10108Vázquez, L. (2008). Complementarities between franchise contract duration and multi-unit propensity in franchise systems. The Service Industries Journal, 28(8), 1093-1105. doi:10.1080/0264206080218794